Culture storage tank for nucleic acid

Abstract

The utility model discloses a culture storage tank for nucleic acid, relates to the technical field of nucleic acid culture, and solves the technical problems that a stable culture environment cannot be provided and a nucleic acid sample cannot be conveniently taken and placed in an existing technical scheme. A fixed column is fixedly installed in the culture tank, a culture frame is rotatably installed on the fixed column, and a tank door is rotatably installed on the side wall face of the culture tank. An accurate and stable temperature control environment is provided for culture and storage of nucleic acid samples; a clean and sterile experimental environment can be created in the culture tank, so that the influence of external pollution on a nucleic acid sample is avoided; the culture frame can be driven by the adjusting motor on the stand column, rotation and adjustment of the culture frame are achieved, and the samples are more convenient to place and take.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid culture storage, in particular to a culture storage tank for nucleic acid. Background Art

[0002] The technical field involved in the utility model is that the nucleic acid culture storage tank is a special equipment for the culture, amplification and storage of nucleic acid (such as DNA and RNA) samples. Its functions include providing ideal temperature, humidity and sterile environment for nucleic acids to ensure the efficiency and stability of samples during the culture process. Through the internal precise control system, it can adjust the conditions to meet the needs of nucleic acid-related experiments such as PCR amplification and enzyme cleavage reaction, and avoid contamination and degradation. In addition, the nucleic acid storage tank can realize sample storage in ultra-low temperature or liquid nitrogen environment, effectively delaying degradation and maintaining the activity and quality of the sample. The advanced equipment also integrates automated processing and data traceability systems, batch sample processing and real-time monitoring to ensure the efficiency and safety of the experiment.

[0003] Nucleic acid culture storage tanks have far-reaching significance in nucleic acid culture and storage technology. They provide stable nucleic acid sample support for basic scientific research such as genomics and molecular biology, and promote the precise development of diagnosis and treatment, such as genetic testing and personalized medicine. Its protection of unstable nucleic acid molecules also lays a technical foundation for the research and development of nucleic acid vaccines and nucleic acid drugs, while reducing the impact of sample degradation on experimental results. In ecological sample protection and environmental genomics research, storage tanks support the long-term preservation of precious biological information and provide complete sample protection for scientific research. By improving nucleic acid sample processing and storage technology, culture storage tanks have become one of the indispensable infrastructures for biotechnology and modern medicine.

[0004] The existing technical solutions have the technical problems of being unable to provide a stable culture environment and being unable to facilitate the taking and placing of nucleic acid samples. Utility Model Content

[0005] In view of the deficiencies of the prior art, the utility model provides a culture storage tank for nucleic acid, which solves the technical problems that the prior art solutions cannot provide a stable culture environment and cannot facilitate the taking and placing of nucleic acid samples.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a culture storage tank for nucleic acid, comprising a main base, a culture tank is fixedly installed on the upper wall of the main base, a fixed column is fixedly installed in the culture tank, a culture rack is rotatably installed on the fixed column, a tank door is rotatably installed on the side wall of the culture tank, a column is fixedly installed on the side wall of the main base, a clean air supply structure is installed on the column, an active temperature control structure is installed on the upper wall of the column, and a culture rack adjustment structure is fixedly installed at the lower part of the column.

[0007] Preferably, a ring light is fixedly installed in the culture tank, and a drainage pipe is fixedly installed at the lower end of the side wall of the culture tank.

[0008] Preferably, the clean air supply structure includes an air supply box, an output pipe is installed at the output end of the air supply box, and the output pipe is connected to the upper side wall of the culture tank; an input pipe is fixedly installed at the input end of the air supply box, and the input end is fixedly installed at the lower end of the side wall of the culture tank; the air supply box is provided with an air intake cavity and an exhaust cavity, the output pipe is connected to the air intake cavity, the input pipe is connected to the exhaust cavity, an air intake fan is fixedly installed in the air intake cavity, and an exhaust fan is fixedly installed in the exhaust cavity.

[0009] Preferably, an air filter plate is fixedly installed in the air inlet cavity, and an adsorbent is fixedly installed in the exhaust cavity.

[0010] Preferably, the active temperature control structure includes a refrigerator and a heat sink, the hot end of the refrigerator is connected to the heat sink, the refrigerator is fixedly installed on the upper wall of the culture tank, the heat sink is fixedly installed on the upper wall of the column, the refrigerator is provided with a refrigeration pipe, the refrigeration pipe is fixedly installed on the upper wall of the culture tank, an auxiliary heating electric pipe is fixedly installed outside the refrigeration pipe, and the culture rack is provided with air holes.

[0011] Preferably, the culture rack adjustment structure includes an adjustment motor, the adjustment motor is fixedly installed between the columns, a driving pulley is fixedly installed on the driving end of the adjustment motor, a main shaft is fixedly installed on the lower wall of the culture rack, a driven pulley is fixedly installed on the main shaft, and a transmission belt is connected to the driving pulley and the driven pulley.

[0012] Beneficial Effects

[0013] The utility model provides a culture and storage tank for nucleic acid. The utility model can realize active regulation of the internal temperature of the culture tank through the combined design of a refrigerator, a heat sink, a refrigeration tube and an auxiliary heating electric heating tube, including rapid cooling and auxiliary heating functions, so as to provide a precise and stable temperature control environment for the culture and storage of nucleic acid samples; the clean air supply structure realizes the input of clean air and the discharge of waste gas, so as to create a clean and sterile experimental environment in the culture tank, avoid the influence of external pollution on the nucleic acid samples, and is particularly suitable for easily degradable RNA molecules; the air holes on the culture rack further optimize the air flow circulation, ensure the circulation of air inside the culture tank, and thus improve the stability of the culture environment; the culture rack can be driven by the adjustment motor on the column to realize the rotation and adjustment of the culture rack, and the placement and use of samples are more convenient; the side wall of the culture tank is equipped with a rotating tank hatch, which is very convenient for taking and placing samples, and also reduces the operation of frequently opening the entire tank body, reducing the risk of external pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the main structure of a culture and storage tank for nucleic acid described in the utility model.

[0015] In the figure: 1. Main base; 2. Culture tank; 3. Fixed column; 4. Culture rack; 5. Tank door; 6. Column; 7. Ring light; 8. Drain pipe; 9. Air box; 10. Output pipe; 11. Input pipe; 12. Intake fan; 13. Exhaust fan; 14. Air filter plate; 15. Adsorbent; 16. Refrigerator; 17. Radiator; 18. Refrigeration pipe; 19. Auxiliary heating pipe; 20. Air vent; 21. Adjustment motor; 22. Driving pulley; 23. Main shaft; 24. Driven pulley; 25. Transmission belt; DETAILED DESCRIPTION

[0016] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the utility model, the technical scheme in the embodiment of the utility model will be clearly and completely described below in combination with the drawings in the embodiment of the utility model. Obviously, the described embodiment is only a part of the embodiment of the utility model, rather than all the embodiments. The detailed description is as follows.

[0017] See also Figure 1 The utility model provides a technical solution: a culture storage tank for nucleic acid, comprising a main base 1, a culture tank 2 is fixedly installed on the upper wall of the main base 1, a fixed column 3 is fixedly installed in the culture tank 2, a culture rack 4 is rotatably installed on the fixed column 3, a tank door 5 is rotatably installed on the side wall of the culture tank 2, a column 6 is fixedly installed on the side wall of the main base 1, a clean air supply structure is installed on the column 6, an active temperature adjustment structure is installed on the upper wall of the column 6, and a culture rack 4 adjustment structure is fixedly installed below the column 6.

[0018] This embodiment is further configured such that a ring light 7 is fixedly installed in the culture tank 2 , and a drainage pipe 8 is fixedly installed at the lower end of the side wall of the culture tank 2 .

[0019] The present embodiment is further configured as follows: the clean air supply structure includes an air supply box 9, an output end of the air supply box 9 is equipped with an output pipe 10, the output pipe 10 is connected to the upper side wall of the culture tank 2, an input end of the air supply box 9 is fixedly equipped with an input pipe 11, the input end is fixedly equipped at the lower end of the side wall of the culture tank 2, the air supply box 9 is provided with an air intake cavity and an exhaust cavity, the output pipe 10 is connected to the air intake cavity, the input pipe 11 is connected to the exhaust cavity, an air intake fan 12 is fixedly installed in the air intake cavity, and an exhaust fan 13 is fixedly installed in the exhaust cavity.

[0020] This embodiment is further configured such that an air filter plate 14 is fixedly installed in the air inlet cavity, and an adsorbent 15 is fixedly installed in the air exhaust cavity.

[0021] The present embodiment is further configured as follows: the active temperature control structure includes a refrigerator 16 and a heat sink 17, the hot end of the refrigerator 16 is connected to the heat sink 17, the refrigerator 16 is fixedly installed on the upper wall of the culture tank 2, the heat sink 17 is fixedly installed on the upper wall of the column 6, a refrigeration pipe 18 is provided on the refrigerator 16, the refrigeration pipe 18 is fixedly installed on the inner upper wall of the culture tank 2, an auxiliary heating electric heating pipe 19 is fixedly installed outside the refrigeration pipe 18, and an air vent 20 is opened on the culture rack 4.

[0022] The present embodiment is further configured such that the adjustment structure of the culture rack 4 includes an adjustment motor 21, the adjustment motor 21 is fixedly installed between the columns 6, a driving pulley 22 is fixedly installed on the driving end of the adjustment motor 21, a main shaft 23 is fixedly installed on the lower wall of the culture rack 4, a driven pulley 24 is fixedly installed on the main shaft 23, and a transmission belt 25 is connected to the driving pulley 22 and the driven pulley 24.

[0023] The detailed connection means are well known in the art; Figure 1 As shown, the first step is to confirm that the culture storage tank, the clean air supply structure (air supply box 9, air intake fan 12, exhaust fan 13), the active temperature control structure (refrigerator 16, heat sink 17, etc.), and the culture rack 4 adjustment system are all in a normal connection state.

[0024] Check whether the air filter plate 14 and the exhaust cavity adsorbent 15 in the clean air supply structure are intact. If there is any damage, replace them.

[0025] Ensure that the drain pipe 8 is unobstructed and free of blockage.

[0026] Turn on the power of the equipment and perform a system self-check to ensure that the temperature control, air supply device, culture rack 4 adjustment and other structures can operate normally.

[0027] Prepare nucleic acid samples that need to be cultured or stored, and ensure that the sample devices (such as test tubes, culture dishes, etc.) meet the equipment requirements to avoid incompatibility.

[0028] The tank hatch 5 on the side wall of the culture tank 2 is rotated to open the tank body.

[0029] The rotation position of the culture rack 4 is adjusted using the adjustment motor 21 according to the sample size or quantity to ensure that the sample rack is aligned to a position that is convenient for placement.

[0030] If necessary, the air holes 20 on the culture rack 4 can be used to adjust the arrangement of the samples to ensure smooth air flow.

[0031] Place the nucleic acid samples (test tubes or microplates, etc.) in order on the culture rack 4 to ensure that the samples are stable and neatly arranged.

[0032] After ensuring that the sample is fixed, close the tank door 5 to keep the interior sealed.

[0033] The culture environment is set through the control panel, including: temperature (adjusting the working status of the refrigerator 16 and the auxiliary heating electric heating tube 19). Airflow circulation intensity (adjusting the speed of the air intake fan 12 and the exhaust fan 13 of the air supply box 9 according to the experimental requirements). If the experiment requires, the ring light 7 function can be turned on to set the light intensity or the opening time. Start the clean air supply system to ensure that the air in the tank is clean. The air intake chamber filters the input air through the air filter plate 14, and the exhaust chamber treats the exhaust gas in the tank through the adsorbent 15. Monitor the culture conditions such as temperature, humidity, and airflow through the system display to ensure that the parameters are stable. Dynamically adjust the environmental conditions according to the experimental requirements.

[0034] After the cultivation or storage is completed, stop the equipment operation and turn off the temperature control system and the air supply system. Confirm whether the waste liquid needs to be discharged from the drain pipe 8. If there is waste liquid, open the drain pipe 8 to drain the waste liquid. Turn the door to open the culture tank 2 and ensure that the air circulation inside stops. Use the adjustment motor 21 to rotate the culture rack 4 and move the sample to an angle that is convenient for removal. Take out the sample carefully to avoid vibration or contamination. Empty the culture rack 4 and perform preliminary cleaning, especially when handling sensitive samples, wipe off the residue. Use a clean cloth and sterile disinfectant to wipe the inside of the culture tank 2, especially the culture rack 4 and the air vents 20, which are prone to accumulate residues. Clean the filter plate 14 and adsorbent 15 in the air supply system, and replace consumables regularly. Empty the waste liquid in the drain pipe 8 and disinfect the pipe. Keep the appearance of the equipment clean to avoid dust accumulation that affects heat dissipation and airflow.

[0035] Turn off the power supply to ensure that the culture storage tank is dry and safe. Avoid opening the door frequently to prevent external impurities from contaminating the internal environment of the storage tank. According to the nature of the experiment, regularly check the performance of the ring light 7 and the airflow system in the culture tank 2. If the nucleic acid sample is sensitive to temperature, be sure to check the cooling and heating effects of the active temperature control system in advance to ensure that it operates within the standard range. In sensitive experiments, a higher frequency of airflow circulation can be selected to improve the cleanliness of the experiment.

[0036] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

[0037] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A culture storage tank for nucleic acid, comprising a main base (1), characterized in that: A culture tank (2) is fixedly mounted on the upper wall of the main base (1), a fixed column (3) is fixedly mounted inside the culture tank (2), a culture rack (4) is rotatably mounted on the fixed column (3), a tank hatch (5) is rotatably mounted on the side wall of the culture tank (2), a column (6) is fixedly mounted on the side wall of the main base (1), a clean air supply structure is mounted on the column (6), an active temperature adjustment structure is mounted on the upper wall of the column (6), and an adjustment structure of the culture rack (4) is fixedly mounted at the lower part of the column (6).

2. A culture storage tank for nucleic acid according to claim 1, characterized in that A ring light (7) is fixedly installed inside the culture tank (2), and a drainage pipe (8) is fixedly installed at the lower end of the side wall of the culture tank (2).

3. A culture storage tank for nucleic acid according to claim 1, characterized in that The clean air supply structure comprises an air supply box (9), an output end of the air supply box (9) is provided with an output pipe (10), the output pipe (10) is connected to the upper side wall of the culture tank (2), an input end of the air supply box (9) is fixedly provided with an input pipe (11), the input end is fixedly provided at the lower end of the side wall of the culture tank (2), the air supply box (9) is provided with an air intake cavity and an exhaust cavity, the output pipe (10) is connected to the air intake cavity, the input pipe (11) is connected to the exhaust cavity, an air intake fan (12) is fixedly provided in the air intake cavity, and an exhaust fan (13) is fixedly provided in the exhaust cavity.

4. A culture storage tank for nucleic acid according to claim 3, characterized in that The air inlet cavity is fixedly installed with an air filter plate (14), and the exhaust cavity is fixedly installed with an adsorbent (15).

5. A culture storage tank for nucleic acid according to claim 1, characterized in that: The active temperature control structure comprises a refrigerator (16) and a heat sink (17), the hot end of the refrigerator (16) is connected to the heat sink (17), the refrigerator (16) is fixedly mounted on the upper wall of the culture tank (2), the heat sink (17) is fixedly mounted on the upper wall of the column (6), a refrigeration pipe (18) is arranged on the refrigerator (16), the refrigeration pipe (18) is fixedly mounted on the inner upper wall of the culture tank (2), an auxiliary heating electric pipe (19) is fixedly mounted outside the refrigeration pipe (18), and an air vent (20) is provided on the culture rack (4).

6. A culture storage tank for nucleic acid according to claim 1, characterized in that The culture rack (4) adjustment structure comprises an adjustment motor (21), the adjustment motor (21) is fixedly mounted between the columns (6), a driving pulley (22) is fixedly mounted on the driving end of the adjustment motor (21), a main shaft (23) is fixedly mounted on the lower wall of the culture rack (4), a driven pulley (24) is fixedly mounted on the main shaft (23), and a transmission belt (25) is connected to the driving pulley (22) and the driven pulley (24).

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