Incubation instrument

Abstract

The utility model provides an incubation instrument. The incubation instrument comprises a shaking table mechanism, an incubation assembly, a plurality of liquid supply assemblies and a plurality of liquid suction assemblies, the incubation assembly is arranged on the shaking table mechanism and is provided with an incubation groove, and the incubation groove is used for loading a sample; the plurality of liquid supply assemblies are controlled to be communicated with the incubation tank respectively and are used for injecting different liquids into the incubation tank; the plurality of liquid suction assemblies are controlled to be communicated with the incubation tank respectively and are used for sucking out liquid from the incubation tank. After different liquids are added, the incubation assembly can move through the shaking table mechanism, so that the liquids fully react with samples, the multiple liquid suction assemblies are communicated with the incubation tank, the reacted liquids can be recycled or discharged, the liquid supply assembly and the liquid suction assemblies are matched with each other, the operation steps are simplified, manual operation is not needed, and the operation efficiency is improved. And automatic protein development can be realized.

Description

Technical Field

[0001] The present application relates to an instrument for protein development, and in particular to an incubation instrument. Background Art

[0002] Currently, protein development requires manual operation on a horizontal shaker. The PVDF membrane that has been transferred is placed in an incubation box and a membrane washing solution is added for cleaning. After cleaning, the washing solution is manually poured out, and then the primary antibody solution and the secondary antibody solution are added in turn. Finally, a blocking solution is added to the incubation box, and the incubation box is placed on a horizontal shaker for a blocking experiment.

[0003] Currently, this experimental method has many steps, and each step takes a long time, requires a timer, and needs to be manually started after one step is completed. Therefore, manual operation of this experiment is very cumbersome and cannot take into account multiple incubation boxes.

[0004] In view of this, the present utility model is proposed. Utility Model Content

[0005] The present application provides an incubation instrument to solve the technical problems of the existing incubation instruments that are cumbersome to operate manually and cannot take care of multiple incubation boxes at the same time.

[0006] The utility model provides an incubation instrument, comprising a shaking table mechanism, an incubation component, a plurality of liquid supply components and a plurality of liquid suction components; the incubation component is arranged on the shaking table mechanism and is provided with an incubation groove, and the incubation groove is used for loading samples; the plurality of liquid supply components are controlled to be connected with the incubation groove respectively, and are used for injecting different liquids into the incubation groove; the plurality of liquid suction components are controlled to be connected with the incubation groove respectively, and are used for sucking liquid out of the incubation groove.

[0007] In this scheme, an incubation tank is opened on the incubation component for loading samples, and the incubation seat is arranged on the shaking table mechanism to drive the incubation component to move. By making multiple liquid supply components connected with the incubation tanks respectively under control, the multiple liquid supply components can inject different liquids into the incubation tanks at different stages. After adding different liquids, the incubation component can be moved by the shaking table mechanism to make the liquid and the sample fully react. Multiple liquid aspiration components are connected with the incubation tanks respectively, and the liquid after the reaction can be recovered or discharged. The liquid supply component and the liquid aspiration component cooperate with each other, which simplifies the operation steps, eliminates the need for manual operation, and can realize automated protein development.

[0008] In a further scheme of the utility model, the incubation instrument also includes multiple liquid supply tubes and multiple liquid suction tubes. The incubation component is provided with a liquid supply groove, and the liquid supply tube is inserted into the liquid supply groove and connected with the incubation groove; the incubation component is also provided with multiple liquid suction holes, which are connected with the incubation groove, and the liquid suction tubes are arranged in the corresponding liquid suction holes. The multiple liquid supply components are respectively connected with the corresponding liquid supply holes, and the multiple liquid suction components are respectively connected with the liquid suction holes.

[0009] In this scheme, by opening a liquid supply trough on the incubation component, the liquid supply tube can be fixed on the liquid supply trough, and by connecting the liquid supply tube with the incubation trough and the corresponding liquid supply component, each liquid supply tube can supply liquid independently to avoid cross contamination, which facilitates the sequential execution of each step in the inspection; similarly, by opening a liquid suction hole on the incubation component and arranging a liquid suction tube in the liquid suction hole to connect the corresponding liquid suction component and the incubation trough, each liquid suction tube can suck liquid independently to avoid cross contamination and affect the purity of the recovered liquid.

[0010] In a further embodiment of the present invention, the incubation tank includes a first tank section and a second tank section, the second tank section is connected to the first tank section, and the bottom wall of the second tank section is lower than the bottom wall of the first tank section; the suction hole is opened on the bottom surface of the second tank section, and the first tank section is used to load samples.

[0011] In a further solution of the present invention, the bottom wall height of the incubation tank is gradually reduced from the first tank section toward the second tank section.

[0012] In this scheme, the first trough section is loaded with samples, and the suction hole is arranged on the bottom surface of the second trough section. By gradually reducing the height of the first trough section toward the bottom of the second trough section, the liquid in the incubation trough can converge to the second trough section, thereby facilitating the liquid to be sucked out of the incubation trough.

[0013] In a further embodiment of the present invention, the multiple liquid supply components include multiple first liquid supply components and at least one second liquid supply component, the multiple first liquid supply components are used to inject different liquids into the incubation tank, and the second liquid supply components are used to inject cleaning liquid into the incubation tank; and / or the multiple liquid aspiration components include at least one recovery liquid aspiration component and at least one waste liquid aspiration component, the recovery liquid aspiration component is used to aspirate the corresponding reaction liquid from the incubation tank, and the waste liquid aspiration component is used to aspirate the waste liquid after cleaning with the cleaning liquid from the incubation tank.

[0014] In this solution, multiple liquid supply components include different first liquid supply components and second liquid supply components, which can realize the function of injecting different liquids and cleaning liquids into the incubation tank to meet the needs of different inspections or operations. At the same time, multiple liquid aspiration components include recovery liquid aspiration components and waste liquid aspiration components, which can realize the recovery and treatment of the liquid in the incubation tank, improving the efficiency and flexibility of the inspection work. The recovery liquid aspiration component can effectively suck out the reaction liquid for subsequent analysis, treatment or reuse; the waste liquid aspiration component can suck out the waste liquid after cleaning with the cleaning liquid, and safely treat it to avoid pollution. This solution can make liquid management more convenient and efficient, and improve the efficiency and operability of the inspection operation in the laboratory.

[0015] In a further scheme of the utility model, the first liquid supply component includes a liquid supply container and a first liquid supply pump, the inlet end of the first liquid supply pump is connected to the liquid supply container, and the outlet end is connected to the corresponding liquid supply pipe; and / or the second liquid supply component includes a liquid supply interface and a second liquid supply pump, the inlet end of the second liquid supply pump is connected to the liquid supply interface, and the outlet end is connected to the corresponding liquid supply pipe; and / or the recovery liquid suction component includes a recovery pump and a recovery container, the outlet end of the recovery pump is connected to the recovery container, and the inlet end is connected to the corresponding liquid suction hole; and / or the waste liquid suction component includes a waste interface and a waste pump, the outlet end of the waste pump is connected to the waste interface, and the inlet end is connected to the corresponding liquid suction hole.

[0016] In this solution, the separation and flexible application of liquid management components are achieved in a modular way. Each liquid supply component or liquid suction component is composed of a container, a pump, a pipeline or a liquid supply interface, so that different functions can be operated independently. The first liquid supply component and the second liquid supply component respectively realize the injection of different types of liquids through their respective pumps and pipeline systems, while the recovery liquid suction component and the waste liquid suction component can also independently recover and process liquids. This modular design makes liquid management more flexible and efficient, and various components can be selectively connected according to inspection requirements to meet different operating requirements.

[0017] In a further scheme of the present invention, multiple first liquid supply components include a primary antibody liquid supply component, a secondary antibody liquid supply component and a sealing liquid supply component; multiple liquid supply components are arranged in groups, and each group of liquid supply components includes a second liquid supply component, a primary antibody liquid supply component, a secondary antibody liquid supply component and a sealing liquid supply component; multiple liquid pipetting components are arranged in groups, and each group of liquid pipetting components includes a recovery liquid pipetting component and a waste liquid pipetting component; there are multiple incubation tanks, and each incubation tank is correspondingly equipped with at least one group of liquid supply components and at least one group of liquid pipetting components.

[0018] In this solution, such a design can realize parallel liquid processing for multiple incubation tanks, and provide different types of liquid supply and recovery processing for each incubation tank. By arranging different types of liquid supply components in groups and equipping them with corresponding second liquid supply components, primary antibody liquid supply components, secondary antibody liquid supply components and sealing liquid supply components, it can be ensured that each incubation tank can receive the required different liquids as needed, and perform corresponding recovery and waste liquid treatment. Based on the above solution, efficient parallel liquid management of multiple incubation tanks can be achieved, which improves the flexibility and efficiency of laboratory operations.

[0019] In a further scheme of the utility model, the incubation instrument also includes a liquid pipette shell, a liquid supply shell, a liquid supply fixing frame and a liquid pipette fixing frame; the liquid supply shell is arranged on the liquid pipette shell, the liquid supply shell forms a liquid supply cavity, the liquid supply fixing frame is arranged in the liquid supply cavity and is used to install multiple liquid supply components; the liquid pipette shell and the incubation component form a liquid pipette cavity, the liquid pipette fixing frame and the shaking table mechanism are arranged in the liquid pipette cavity, and the liquid pipette fixing frame is used to install multiple liquid pipette components.

[0020] In this scheme, by integrating liquid management components such as a liquid pipetting component and a liquid supply component into an incubation instrument, the convenience and efficiency of the inspection operation are improved. By setting the liquid pipetting shell, the liquid supply shell, the liquid supply fixing frame and the liquid pipetting fixing frame, the fixing and installation of the liquid pipetting component and the liquid supply component are realized. The liquid supply shell and the liquid pipetting shell form a liquid supply cavity and a liquid pipetting cavity respectively, so that the liquid management component can be installed therein in an orderly manner, which can play a certain protective effect on the liquid pipetting component and the liquid supply component. The setting of the liquid supply fixing frame and the liquid pipetting fixing frame provides a stable installation support for multiple liquid supply components and liquid pipetting components, ensuring that they will not shake or fall off during use, and ensuring the stable inspection. The liquid supply shell and the liquid pipetting shell are set separately, and the liquid supply component is set in the liquid supply shell, which is convenient for managing the liquid pipetting component and the liquid supply component separately.

[0021] In a further solution of the present invention, the first liquid supply pump and the second liquid supply pump are both arranged on the liquid suction fixing frame; an opening is opened on the liquid supply shell, and the liquid supply container is arranged on the liquid suction fixing frame after passing through the liquid supply shell from the opening, and the liquid supply container at least partially protrudes from the liquid supply shell.

[0022] In a further scheme of the utility model, the rocking table mechanism includes: a fixed seat connected to the liquid suction shell; a control rod rotatably connected to the fixed seat; a cam eccentrically connected to the control rod; and a support frame, on which the incubation assembly is arranged; the support frame is formed with an activity space, the cam is arranged in the activity space and pushes the support frame to move when it rotates eccentrically.

[0023] In this solution, the fixed seat is connected to the liquid aspiration housing, ensuring a stable connection between the shaking table mechanism and the incubation instrument without shaking or falling off. The control rod is rotatably connected to the fixed seat, and the cam is eccentrically connected to the control rod. Through such a design, the cam can push and move the support frame when rotating, so that the incubation components can be fully mixed and stirred on the shaking table. The design of the support frame forms an activity space, in which the cam is set and pushes the support frame to move when rotating eccentrically, so that the support frame can be shaken according to the set path and speed, ensuring the full mixing and reaction of the liquid during the incubation process.

[0024] In a further solution of the utility model, the incubation assembly includes an incubation seat and a plurality of incubation trays; the incubation seat is connected to the shaking table mechanism, the incubation trays are arranged on the incubation seat, and the incubation trays are provided with a plurality of incubation slots.

[0025] In this solution, by arranging a plurality of incubation trays on the incubation seat and arranging a plurality of incubation slots on the incubation trays, the entire incubation instrument can perform protein development on a plurality of samples, thereby effectively improving the inspection efficiency.

[0026] In summary, the incubation instrument provided in this application has at least the following beneficial effects:

[0027] The present application provides an incubation tank on the incubation component for loading samples, and an incubation seat is arranged on a shaking table mechanism to drive the incubation component to move. By making multiple liquid supply components connected to the incubation tanks respectively under control, the multiple liquid supply components can inject different liquids into the incubation tanks at different stages. After adding different liquids, the incubation component can be moved by the shaking table mechanism to allow the liquid and the sample to fully react. Multiple liquid aspiration components are connected to the incubation tanks respectively, and the liquid after the reaction can be recovered or discharged. The liquid supply components and the liquid aspiration components cooperate with each other, which simplifies the operation steps, eliminates the need for manual operation, and can realize automated protein development. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific implementation of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific implementation or the prior art description. Obviously, the drawings described below are some implementations of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0029] Figure 1 A schematic diagram of the structure of the incubation instrument provided in the embodiment of the present application;

[0030] Figure 2 A schematic diagram of an incubation assembly and a liquid supply tube provided in an embodiment of the present application;

[0031] Figure 3 A schematic diagram of the structure of the incubation tray and the pipette provided in the embodiment of the present application;

[0032] Figure 4 A schematic diagram of the internal structure of the liquid supply housing provided in an embodiment of the present application;

[0033] Figure 5 A schematic diagram of the internal structure of the liquid suction housing provided in an embodiment of the present application;

[0034] Figure 6A schematic diagram of the internal structure of the liquid aspiration component and the incubation component provided in the embodiment of the present application; and

[0035] Figure 7 A schematic diagram of the structure of the rocking table mechanism provided in an embodiment of the present application.

[0036] The reference numerals are as follows:

[0037] 100, incubation assembly; 110, incubation tray; 110A, incubation tank; 110B, first tank section; 110C, second tank section; 110D, liquid supply tank; 110E, liquid suction hole; 120, incubation seat;

[0038] 200, liquid supply assembly; 210, first liquid supply assembly; 211, first liquid supply pump; 212, liquid supply container; 220, second liquid supply assembly; 221, second liquid supply pump; 222, liquid supply interface; 210A, primary antibody liquid supply assembly; 210B, secondary antibody liquid supply assembly; 210C, blocking liquid liquid supply assembly;

[0039] 300, liquid suction component; 310, recycling liquid suction component; 311, recycling liquid suction pump; 312, recycling container; 320, discarding liquid suction component; 321, discarding liquid suction pump; 322, discarding interface;

[0040] 400, liquid supply housing; 400A, opening;

[0041] 500, rocking bed mechanism; 510, fixing seat; 520, control rod; 530, cam; 540, supporting frame;

[0042] 600, liquid aspiration housing; 700, liquid supply fixing frame; 800, liquid aspiration fixing frame; 910, liquid supply pipe; 920, liquid aspiration pipe. DETAILED DESCRIPTION

[0043] In the description of the present application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like appear to indicate the orientation or position relationship, unless otherwise specified, they are understood to be based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present application.

[0044] In addition, if there is a feature defined as "first" or "second", it is only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Features defined as "first" or "second" may explicitly or implicitly include at least one of the defined features. If there is a description of "plurality", the general meaning is to include at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0045] In this application, unless otherwise clearly specified and limited, the terms such as "installed", "connected", "connected", "fixed" and so on should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection, it can be a direct connection, or it can be an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0046] In the description of this specification, if the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0047] Please refer to Figure 1 The utility model provides an incubation instrument, including a shaking table mechanism 500, an incubation component 100, a plurality of liquid supply components 200 and a plurality of liquid suction components 300; the incubation component 100 is arranged on the shaking table mechanism 500 and is provided with an incubation groove 110A, and the incubation groove 110A is used to load samples; the plurality of liquid supply components 200 are controlled to be connected with the incubation groove 110A respectively, and are used to inject different liquids into the incubation groove 110A; the plurality of liquid suction components 300 are controlled to be connected with the incubation groove 110A respectively, and are used to suck out liquid from the incubation groove 110A.

[0048] In this solution, an incubation tank 110A is opened on the incubation component 100 for loading samples, and the incubation component 100 is set on a rocking table mechanism 500 so that the rocking table mechanism 500 can drive the incubation component 100 to move. A plurality of liquid supply components 200 are controlled to be connected to the incubation tank 110A respectively, so that the plurality of liquid supply components 200 can inject different liquids into the incubation tank 110A at different stages. After adding different liquids, the incubation component 100 can be moved by the rocking table mechanism 500, so that the liquid and the sample can fully react. A plurality of liquid aspiration components 300 are connected to the incubation tank 110A respectively, and the liquid after the reaction can be recovered or discharged. The liquid supply component 200 and the liquid aspiration component 300 cooperate with each other, which simplifies the operation steps, eliminates the need for manual operation, and can realize automated protein development.

[0049] It should be noted that in order to better show the relative positions of the various components, the pipes used to connect the various components are omitted in each schematic diagram.

[0050] Please refer to Figure 2 and Figure 3 In a further embodiment of the present invention, the incubation instrument further includes a plurality of liquid supply tubes 910 and a plurality of liquid suction tubes 920. The incubation component 100 is provided with a liquid supply groove 110D. The liquid supply tube 910 is inserted into the liquid supply groove 110D and is connected with the incubation groove 110A. The incubation component 100 is further provided with a plurality of liquid suction holes 110E. The liquid suction holes 110E are connected with the incubation groove 110A. The liquid suction tubes 920 are arranged in the corresponding liquid suction holes 110E. The plurality of liquid supply components 200 respectively inject the corresponding connecting liquid into the incubation groove 110A through the corresponding liquid supply tubes 910, and the plurality of liquid suction components 300 respectively suck out the liquid from the incubation groove 110A through the corresponding liquid suction tubes 920. In this scheme, by opening a liquid supply groove 110D on the incubation component 100, the liquid supply tube 910 can be fixed on the liquid supply groove 110D, and by connecting the liquid supply tube 910 with the incubation groove 110A and the corresponding liquid supply component 200, each liquid supply tube 910 can supply liquid independently to avoid cross contamination, which facilitates the sequential execution of each step in the inspection; similarly, by opening a liquid suction hole 110E on the incubation component 100, and arranging a liquid suction tube 920 in the liquid suction hole 110E to connect the corresponding liquid suction component 300 with the incubation groove 110A, each liquid suction tube 920 can suck liquid independently to avoid cross contamination and affect the purity of the recovered liquid.

[0051] In a further embodiment of the present invention, the incubation tank 110A includes a first tank section 110B and a second tank section 110C, the second tank section 110C is connected to the first tank section 110B, and the bottom wall of the second tank section 110C is lower than the bottom wall of the first tank section 110B; the suction hole 110E is opened on the bottom surface of the second tank section 110C, and the first tank section 110B is used to load samples.

[0052] In a further embodiment of the present invention, the bottom wall height of the incubation tank 110A is gradually reduced from the first tank section 110B toward the second tank section 110C.

[0053] In this scheme, the first trough section 110B is loaded with the sample, and the suction hole 110E is arranged on the bottom surface of the second trough section 110C. By gradually reducing the height of the first trough section 110B toward the bottom of the second trough section 110C, the liquid in the incubation trough 110A can converge to the second trough section 110C, thereby facilitating the liquid to be sucked out of the incubation trough 110A.

[0054] Please refer to Figure 4-Figure 5 In a further embodiment of the present invention, multiple liquid supply components 200 include multiple first liquid supply components 210 and at least one second liquid supply component 220, multiple first liquid supply components 210 are used to inject different liquids into the incubation tank 110A, and the second liquid supply component 220 is used to inject cleaning liquid into the incubation tank 110A; and / or multiple liquid aspiration components 300 include at least one recovery liquid aspiration component 310 and at least one waste liquid aspiration component 320, the recovery liquid aspiration component 310 is used to aspirate the corresponding reaction liquid from the incubation tank 110A, and the waste liquid aspiration component 320 is used to aspirate the waste liquid after cleaning with the cleaning liquid from the incubation tank 110A.

[0055] In this solution, through multiple liquid supply components 200 including different first liquid supply components 210 and second liquid supply components 220, the function of injecting different liquids and cleaning liquids into the incubation tank 110A can be realized to meet the needs of different inspections or operations. At the same time, multiple liquid pipetting components 300 include a recovery liquid pipetting component 310 and a discarded liquid pipetting component 320, which can realize the recovery and treatment of the liquid in the incubation tank 110A, thereby improving the efficiency and flexibility of the inspection work. The recovery liquid pipetting component 310 can effectively suck out the reaction liquid for subsequent analysis, treatment or reuse; the discarded liquid pipetting component 320 can suck out the waste liquid after cleaning with the cleaning liquid, and safely treat it to avoid pollution. This solution can make liquid management more convenient and efficient, and improve the efficiency and operability of the inspection operation in the laboratory.

[0056] In a further embodiment of the present invention, the first liquid supply assembly 210 includes a liquid supply container 212 and a first liquid supply pump 211, the inlet end of the first liquid supply pump 211 is connected to the liquid supply container 212, and the outlet end is connected to the corresponding liquid supply pipe 910; and / or the second liquid supply assembly 220 includes a liquid supply interface 222 and a second liquid supply pump 221, the inlet end of the second liquid supply pump 221 is connected to the liquid supply interface 222, and the outlet end is connected to the corresponding liquid supply pipe 910; and / or the recovery and suction assembly 310 includes a recovery pump 311 and a recovery container 312, the outlet end of the recovery pump 311 is connected to the recovery container 312, and the inlet end is connected to the corresponding suction hole 110E; And / or the waste liquid aspiration component 320 includes a waste interface 322 and a waste pump 321, the outlet end of the waste pump 321 is connected to the waste interface 322, and the inlet end is connected to the corresponding liquid aspiration hole 110E. In actual use, the waste interface 322 needs to be externally connected to a waste liquid collection container, or directly connected to a waste liquid treatment device; the liquid supply interface 222 is connected to a container with a target solution, that is, the first liquid supply component 210 and the recovery liquid aspiration component are suitable for containers with a smaller capacity after liquid supply or liquid aspiration, and can be directly installed on the incubation instrument. The second liquid supply component 220 and the waste liquid aspiration component 320 are the opposite. The container requires a larger capacity and is then installed outside the equipment.

[0057] In this solution, the separation and flexible application of liquid management components are achieved in a modular way. Each liquid supply component 200 or liquid suction component 300 is composed of a container, a pump, a pipeline or a liquid supply interface 222, so that different functions can be operated independently. The first liquid supply component 210 and the second liquid supply component 220 respectively realize the injection of different types of liquids through their respective pumps and pipeline systems, while the recovery and suction components and the waste suction components 320 can also independently recover and process liquids. This modular design makes liquid management more flexible and efficient, and various components can be selectively connected according to inspection requirements to meet different operating requirements.

[0058] In a further embodiment of the present invention, multiple first liquid supply components 210 include a primary antibody liquid supply component 210A, a secondary antibody liquid supply component 210B and a sealing liquid supply component 210C; multiple liquid supply components 200 are arranged in groups, and each group of liquid supply components 200 includes a second liquid supply component 220, a primary antibody liquid supply component 210A, a secondary antibody liquid supply component 210B and a sealing liquid supply component 210C; multiple liquid pipetting components 300 are arranged in groups, and each group of liquid pipetting components 300 includes a recovery liquid pipetting component 310 and a waste liquid pipetting component 320; there are multiple incubation tanks 110A, and each incubation tank 110A is correspondingly equipped with at least one group of liquid supply components and at least one group of liquid pipetting components 300.

[0059] In this solution, such a design can realize parallel liquid processing of multiple incubation tanks 110A, and provide different types of liquid supply and recovery processing for each incubation tank 110A. By arranging different types of liquid supply components 200 in groups, and equipping corresponding second liquid supply components 220, primary antibody liquid supply components 210A, secondary antibody liquid supply components 210B and sealing liquid supply components 210C, it can be ensured that each incubation tank 110A can receive the required different liquids as needed, and perform corresponding recovery and waste liquid treatment. Based on the above solution, efficient parallel liquid management of multiple incubation tanks 110A can be achieved, which improves the flexibility and efficiency of laboratory operations.

[0060] In a further scheme of the present utility model, the incubation instrument also includes a liquid pipetting shell 600, a liquid supply shell 400, a liquid supply fixing frame 700 and a liquid pipetting fixing frame 800; the liquid supply shell 400 is arranged on the liquid pipetting shell 600, the liquid supply shell 400 forms a liquid supply cavity, the liquid supply fixing frame 700 is arranged in the liquid supply cavity and is used to install multiple liquid supply components 200; the liquid pipetting shell 600 and the incubation component 100 form a liquid pipetting cavity, the liquid pipetting fixing frame 800 and the shaking table mechanism 500 are arranged in the liquid pipetting cavity, and the liquid pipetting fixing frame 800 is used to install multiple liquid pipetting components 300.

[0061] In this scheme, by adding liquid management components such as the liquid pipetting assembly 300 and the liquid supply assembly 200 to the incubation instrument, the convenience and efficiency of the inspection operation are improved. By setting the liquid pipetting housing 600, the liquid supply housing 400, the liquid supply fixing frame 700 and the liquid pipetting fixing frame 800, the fixing and installation of the liquid pipetting assembly 300 and the liquid supply assembly 200 are realized. The liquid supply housing 400 and the liquid pipetting housing 600 respectively form a liquid supply cavity and a liquid pipetting cavity, so that the liquid management components can be installed therein in an orderly manner, which can provide a certain protective effect on the liquid pipetting assembly 300 and the liquid supply assembly 200. The setting of the liquid supply fixing frame 700 and the liquid pipetting fixing frame 800 provides a stable installation support for multiple liquid supply assemblies 200 and the liquid pipetting assembly 300, ensuring that they will not shake or fall off during use, and ensuring the stable progress of the inspection. The liquid supply housing 400 and the liquid suction housing 600 are separately arranged, and the liquid supply assembly 200 is arranged in the liquid supply housing 400, so that the liquid suction assembly 300 and the liquid supply assembly 200 can be managed separately.

[0062] Please refer to further Figure 1 A liquid supply support frame 540 (not marked in the figure) is provided on the liquid supply shell 400, and the liquid supply shell 400 is installed in the liquid suction cavity through the liquid supply support frame 540. There is also a power interface on the back of the liquid suction shell 600, and a waste liquid interface is also provided on the back of the liquid suction shell 600.

[0063] In a further solution of the present invention, the first liquid supply pump 211 and the second liquid supply pump 221 are both arranged on the liquid suction fixing frame 800; an opening 400A is opened on the liquid supply shell 400, and the liquid supply container 212 is arranged on the liquid suction fixing frame 800 after passing through the liquid supply shell 400 from the opening 400A, and the liquid supply container 212 at least partially protrudes from the liquid supply shell 400.

[0064] Please refer to Figure 7 In a further embodiment of the present invention, the rocking table mechanism 500 includes: a fixed seat 510, connected to the liquid suction shell 600; a control rod 520, rotatably connected to the fixed seat 510; a cam 530, eccentrically connected to the control rod 520; and a support frame 540, on which the incubation component 100 is arranged; the support frame 540 is formed with an activity space, and the cam 530 is arranged in the activity space and pushes the support frame 540 to move when it rotates eccentrically.

[0065] In this solution, the fixed seat 510 is connected to the liquid aspiration housing 600, ensuring the stable connection between the shaking table mechanism 500 and the incubation instrument, and there will be no shaking or falling off. The control rod 520 is rotatably connected to the fixed seat 510, and the cam 530 is eccentrically connected to the control rod 520. Through such a design, the cam 530 can push and move the support frame 540 when rotating, so that the incubation component 100 can be fully mixed and stirred on the shaking table mechanism 500. The design of the support frame 540 forms an activity space, and the cam 530 is arranged therein and pushes the support frame 540 to move when rotating eccentrically, so that the support frame 540 can be shaken according to the set path and speed, ensuring the full mixing and reaction of the liquid during the incubation process.

[0066] In order to better realize automation, an electric control component (not shown in the figure) can also be set in the liquid suction housing 600. The electric control component controls the solution in the incubation tank 110A by controlling the first liquid supply pump 211, the second liquid supply pump 221, the recovery liquid suction pump 311 or the waste liquid suction pump 321. The electric control component is connected to the power interface. The rocking table mechanism 500 should also include a motor, which is arranged in the liquid suction cavity. The control rod 520 is arranged on the power output end of the motor. The control rod 520 is driven by the motor to rotate, thereby driving the incubation component 100 to move. The motor is also electrically connected to the electric control component to realize automatic control.

[0067] Please continue to refer to Figure 4-Figure 6 In a further embodiment of the present invention, the incubation assembly 100 includes an incubation seat 120 and a plurality of incubation trays 110; the incubation seat 120 is connected to the shaking table mechanism 500, and the incubation trays 110 are arranged on the incubation seat 120, and the incubation trays 110 are provided with a plurality of incubation slots 110A.

[0068] In this solution, by arranging a plurality of incubation trays 110 on the incubation seat 120 and arranging a plurality of incubation slots 110A on the incubation trays 110, the entire incubation instrument can perform protein development on a plurality of samples, thereby effectively improving the inspection efficiency.

[0069] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. An incubation instrument, characterized in that: It comprises a shaking table mechanism (500), an incubation component (100), a plurality of liquid supply components (200) and a plurality of liquid aspiration components (300); The incubation component (100) is arranged on the shaking table mechanism (500) and is provided with an incubation tank (110A), wherein the incubation tank (110A) is used for loading samples; The plurality of liquid supply components (200) are controlled to be in communication with the incubation tank (110A) respectively, and are used to inject different liquids into the incubation tank (110A); The plurality of liquid suction components (300) are controlled to be in communication with the incubation tank (110A) respectively, and are used to suck out liquid from the incubation tank (110A); The incubation instrument further comprises a plurality of liquid supply tubes (910) and a plurality of liquid pipettes (920), wherein the plurality of liquid supply components (200) are respectively connected to the corresponding liquid supply tubes (910), and the plurality of liquid pipette components (300) are respectively connected to the corresponding liquid pipettes (920); The incubation assembly (100) is provided with a liquid supply tank (110D), and the liquid supply pipe (910) is inserted into the liquid supply tank (110D) and communicated with the incubation tank (110A); The incubation component (100) is further provided with a plurality of liquid suction holes (110E), wherein the liquid suction holes (110E) are connected to the incubation tank (110A), and the liquid suction tubes (920) are arranged through the corresponding liquid suction holes (110E).

2. The incubation instrument according to claim 1, characterized in that: The incubation tank (110A) comprises a first tank section (110B) and a second tank section (110C), wherein the second tank section (110C) is connected to the first tank section (110B), and a bottom wall of the second tank section (110C) is lower than a bottom wall of the first tank section (110B); The first groove section (110B) is used for loading samples, and the liquid aspiration hole (110E) is opened on the bottom surface of the second groove section (110C).

3. The incubation instrument according to claim 2, characterized in that: The height of the bottom wall of the incubation tank (110A) is gradually reduced in a direction from the first tank section (110B) toward the second tank section (110C).

4. The incubation instrument according to claim 1, characterized in that: The plurality of liquid supply components (200) include a plurality of first liquid supply components (210) and at least one second liquid supply component (220), wherein the plurality of first liquid supply components (210) are used to inject different liquids into the incubation tank (110A), and the second liquid supply component (220) is used to inject a cleaning liquid into the incubation tank (110A); and / or The multiple liquid aspiration components (300) include at least one recovery liquid aspiration component (310) and at least one waste liquid aspiration component (320), wherein the recovery liquid aspiration component (310) is used to aspirate the corresponding reaction liquid from the incubation tank (110A), and the waste liquid aspiration component (320) is used to aspirate the waste liquid after cleaning with the cleaning liquid from the incubation tank (110A).

5. The incubation instrument according to claim 4, characterized in that: The first liquid supply component (210) comprises a liquid supply container (212) and a first liquid supply pump (211), wherein the inlet end of the first liquid supply pump (211) is connected to the liquid supply container (212), and the outlet end of the first liquid supply pump (211) is connected to the corresponding liquid supply pipe (910); and / or The second liquid supply component (220) comprises a liquid supply interface (222) and a second liquid supply pump (221), the inlet end of the second liquid supply pump (221) is connected to the liquid supply interface (222), and the outlet end is connected to the corresponding liquid supply pipe (910); and / or The recovery and liquid suction component (310) comprises a recovery pump (311) and a recovery container (312), wherein the outlet end of the recovery pump (311) is connected to the recovery container (312), and the inlet end of the recovery pump (311) is connected to the corresponding liquid suction hole (110E); and / or The waste liquid suction component (320) comprises a waste interface (322) and a waste pump (321), wherein the outlet end of the waste pump (321) is connected to the waste interface (322), and the inlet end is connected to the corresponding liquid suction hole (110E).

6. The incubation instrument according to claim 4, characterized in that: The plurality of first liquid supply components (210) include a primary antibody liquid supply component (210A), a secondary antibody liquid supply component (210B) and a blocking liquid supply component (210C); A plurality of the liquid supply components (200) are arranged in groups, and each group of the liquid supply components (200) comprises the second liquid supply component (220), the primary antibody liquid supply component (210A), the secondary antibody liquid supply component (210B), and the blocking liquid supply component (210C); A plurality of the liquid absorbing components (300) are arranged in groups, and each group of the liquid absorbing components (300) includes the liquid recovery component (310) and the liquid waste component (320); There are multiple incubation tanks (110A), and each incubation tank (110A) is correspondingly equipped with at least one group of the liquid supply components (200) and at least one group of the liquid aspiration components (300).

7. The incubation instrument according to any one of claims 1 to 6, characterized in that: The incubation instrument further comprises a liquid aspiration housing (600), a liquid supply housing (400), a liquid supply fixing frame (700) and a liquid aspiration fixing frame (800); The liquid supply housing (400) is arranged on the liquid suction housing (600), the liquid supply housing (400) forms a liquid supply cavity, and the liquid supply fixing frame (700) is arranged in the liquid supply cavity and is used to install a plurality of the liquid supply assemblies (200); The liquid aspiration shell (600) and the incubation component (100) form a liquid aspiration cavity, the liquid aspiration fixing frame (800) and the rocking table mechanism (500) are arranged in the liquid aspiration cavity, and the liquid aspiration fixing frame (800) is used to install a plurality of the liquid aspiration components (300).

8. The incubation instrument according to claim 7, characterized in that: The rocking table mechanism (500) comprises: A fixing seat (510) connected to the liquid suction housing (600); A control rod (520) rotatably connected to the fixing seat (510); a cam (530) eccentrically connected to the control rod (520); and A support frame (540), wherein the incubation assembly (100) is arranged on the support frame (540); The support frame (540) is formed with an activity space, and the cam (530) is arranged in the activity space and pushes the support frame (540) to move when it rotates eccentrically.

9. The incubation instrument according to claim 8, characterized in that: The incubation assembly (100) comprises an incubation seat (120) and a plurality of incubation trays (110); The incubation seat (120) is connected to the shaking table mechanism (500), the incubation tray (110) is arranged on the incubation seat (120), and the incubation tray (110) is provided with a plurality of incubation grooves (110A).

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