Pipetting device and phage display device with same

Abstract

The utility model provides a pipetting device and a phage display device with the pipetting device, and the pipetting device comprises a first pipetting needle, a second pipetting needle, a first delivery pump, a second delivery pump and a flow path switching valve, the flow path switching valve is provided with a small-flow pipetting flow path and a large-flow pipetting flow path, the small-flow pipetting flow path is communicated with the first delivery pump and the first pipetting needle, the large-flow pipetting flow path is communicated with the second delivery pump, the first pipetting needle and the second pipetting needle, and the flow path switching valve can be controlled to be switched between the small-flow pipetting flow path and the large-flow pipetting flow path; the pumping flow of the second conveying pump is larger than that of the first conveying pump. According to the utility model, a small-flow pipetting flow path can be selected according to corresponding process requirements to ensure accurate pipetting of liquids such as reagents and sample liquids, and a large-flow pipetting flow path can be selected to ensure high-efficiency transfer of the liquids such as the reagents and the sample liquids, so that the operation efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of phage display equipment design, and in particular relates to a liquid transfer device and a phage display device having the same. Background Art

[0002] Phage is a type of tiny life form that infects and replicates inside bacteria. Using synthetic biology tools, the genetic information of phage can be reprogrammed, making phage or infected bacteria an important tool for drug screening, protein optimization, and biomarker screening.

[0003] Phage display technology is one of the two major development platforms for all approved antibody drugs in the world. Due to its mature research system, low cost, and simple operation, phage display technology has attracted widespread attention from researchers and has extremely important commercial value.

[0004] In the field of antibody preparation, phage display technology provides a simple and fast genetic engineering antibody preparation route for fully human recombinant antibodies: a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes are amplified from human immune cells using PCR technology and expressed on the phage surface in the form of fusion proteins.

[0005] Various types of antibodies can be screened through phage display technology. Compared with monoclonal antibodies produced by immunizing animals, monoclonal antibodies produced using this technology have the advantages of higher efficiency and faster production.

[0006] The current phage display technology is implemented by a combination of manual operations using equipment such as full-temperature vibration incubators, centrifuges, and cloning panning equipment. The aforementioned equipment is a single-function, independent device that requires operator participation, which leads to the risk of cross-contamination during the culture and panning process. Manual participation also leads to manual selection bias, reducing the stability of the process and results. In addition, the independent application of single-function modules and the low degree of equipment integration result in time-consuming and costly phage screening and display. It should be noted that the pipetting devices in the prior art are mostly used in application conditions with a relatively narrow flow range (single small flow or single larger flow). For application conditions where both small-volume pipetting and large-volume pipetting are required, the current pipetting devices cannot meet the dual needs of small-volume pipetting and large-volume rapid pipetting. Utility Model Content

[0007] Therefore, the technical problem to be solved by the present invention is to provide a pipetting device and a phage display device having the same, so as to solve the technical problem that the pipetting device in the prior art cannot meet the dual requirements of small-volume pipetting and large-volume rapid pipetting at the same time.

[0008] In order to solve the above problems, the utility model provides a pipetting device, including a first pipetting needle, a second pipetting needle, a first delivery pump, a second delivery pump and a flow path switching valve, the flow path switching valve having a small-flow pipetting flow path connecting the first delivery pump and the first pipetting needle, and a large-flow pipetting flow path connecting the second delivery pump, the first pipetting needle and the second pipetting needle, and the flow path switching valve can be controlled to switch between the small-flow pipetting flow path and the large-flow pipetting flow path, and the pumping flow of the second delivery pump is greater than the pumping flow of the first delivery pump.

[0009] In some embodiments, the flow path switching valve has a first port, a second port, and a third port, the first pipette needle is connected to the first port, the first delivery pump is controllably connected to the second port, and the second pipette needle is controllably connected to the third port via the second delivery pump.

[0010] In some embodiments, the first delivery pump is a plunger pump, and the second delivery pump is a peristaltic pump.

[0011] In some embodiments, the pipetting device also includes a pipetting bracket, the second pipetting needle is assembled on the first side of the pipetting bracket and can be driven to rise and fall, the first pipetting needle is assembled on the first side via a first linear displacement component and can be driven to rise and fall, and the linear displacement direction of the first linear displacement component is parallel to the horizontal length direction of the pipetting bracket.

[0012] In some embodiments, the first linear displacement assembly includes a first linear displacement drive assembly, which includes a transverse drive motor, an idler wheel and a synchronous belt, the synchronous belt is tensioned between the output driving wheel of the transverse drive motor and the idler wheel, and the first pipette needle is fixedly connected to the synchronous belt.

[0013] In some embodiments, the first linear displacement assembly further includes a linear guide assembly, which includes a transverse linear guide rail fixedly connected to the first side surface and a transverse slider fixedly connected to the first pipette needle, wherein the transverse slider is slidably connected to the transverse linear guide rail.

[0014] In some embodiments, the linear guide assembly has two groups spaced apart one above the other.

[0015] In some embodiments, a pipette gun assembly is further assembled on the first side, and the aspiration and discharge capacity of the pipette gun assembly is smaller than the aspiration and discharge capacity of the first pipette needle and the second pipette needle.

[0016] In some embodiments, the pipette gun assembly is connected to the first side assembly via a second linear displacement drive assembly, and the driving direction of the second linear displacement drive assembly is parallel to the driving direction of the first linear displacement drive assembly; and / or, the pipette gun assembly and the first pipette needle share the linear guide assembly.

[0017] The utility model also provides a phage display device, comprising the above-mentioned liquid transfer device.

[0018] The utility model provides a pipetting device and a phage display device having the same. The flow path switching valve and the first pipetting needle, the second pipetting needle, the first delivery pump and the second delivery pump can respectively form a small-flow pipetting flow path and a large-flow pipetting flow path. In this way, according to the corresponding process requirements, a small-flow pipetting flow path can be selected to ensure the precise pipetting of liquids such as reagents and sample liquids, and a large-flow pipetting flow path can be selected to ensure the efficient transfer of liquids such as reagents and sample liquids, thereby improving the operating efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the principle of the liquid transfer device according to an embodiment of the utility model;

[0020] Figure 2 It is a schematic diagram of the three-dimensional structure of the liquid transfer device according to an embodiment of the utility model.

[0021] The reference numerals are as follows:

[0022] 71. First pipetting needle; 72. Second pipetting needle; 73. First delivery pump; 74. Second delivery pump; 75. Flow path switching valve; 751. First port; 752. Second port; 753. Third port; 761. Pipetting bracket; 7621. Transverse drive motor; 7622. Idle wheel; 7623. Synchronous belt; 763. Linear guide assembly; 77. Pipette gun assembly; 771. Second linear displacement drive assembly. DETAILED DESCRIPTION

[0023] See also Figure 1 to Figure 2 As shown, according to an embodiment of the utility model, a pipetting device is provided, including a first pipetting needle 71, a second pipetting needle 72, a first delivery pump 73, a second delivery pump 74 and a flow path switching valve 75, the flow path switching valve 75 having a small flow rate pipetting flow path connecting the first delivery pump 73 and the first pipetting needle 71, and a large flow rate pipetting flow path connecting the second delivery pump 74, the first pipetting needle 71 and the second pipetting needle 72, and the flow path switching valve 75 can be controlled to switch between the small flow rate pipetting flow path and the large flow rate pipetting flow path, the pumping flow rate of the second delivery pump 74 is greater than the pumping flow rate of the first delivery pump 73, and the aforementioned first pipetting needle 71 and the second pipetting needle 72 can be implemented, for example, by hollow steel needles commonly used in the industry.

[0024] In this technical solution, the flow path switching valve 75 and the aforementioned first pipetting needle 71, second pipetting needle 72, first delivery pump 73 and second delivery pump 74 can respectively form a small-flow pipetting flow path and a large-flow pipetting flow path. In this way, according to the corresponding process requirements, a small-flow pipetting flow path can be selected to ensure the precise pipetting of liquids such as reagents and sample liquids, and a large-flow pipetting flow path can be selected to ensure the efficient transfer of liquids such as reagents and sample liquids, thereby improving operational efficiency.

[0025] In a specific embodiment, the flow path switching valve 75 has a first port 751, a second port 752, and a third port 753. The first pipette needle 71 is connected to the first port 751, the first delivery pump 73 is controllably connected to the second port 752, and the second pipette needle 72 is controllably connected to the third port 753 via the second delivery pump 74. The aforementioned flow path switching valve 75 objectively forms a controllable on-off three-way valve, which can specifically adopt an electromagnetic three-way valve to achieve smooth and automatic switching between the two flow paths.

[0026] As a preferred embodiment, the first delivery pump 73 is a plunger pump, which has a small pumping flow rate and is suitable for the precise transfer of smaller volumes of reagents or sample liquids. The second delivery pump 74 is a peristaltic pump, which has a simple structure and a large pumping flow rate range and can meet the requirements of efficient transfer of large volumes of reagents or sample liquids. At the same time, it can also be controlled to rotate forward and reversely to achieve pumping from the first pipette needle 71 to the second pipette needle 72 or from the second pipette needle 72 to the first pipette needle 71.

[0027] In some embodiments, the pipetting device also includes a pipetting bracket 761, which serves as an assembly carrier for various pipetting components (such as the aforementioned first pipetting needle 71, the second pipetting needle 72, etc.). Specifically, the second pipetting needle 72 is assembled on the first side surface of the pipetting bracket 761 and can be driven to rise and fall. The first pipetting needle 71 is assembled on the first side surface via a first linear displacement component (not marked in the figure) and can be driven to rise and fall. The linear displacement direction of the first linear displacement component is parallel to the horizontal length direction of the pipetting bracket 761. It should be noted that the second pipetting needle 72 will not produce lateral displacement in the aforementioned linear displacement direction, that is, the lateral position of the second pipetting needle 72 is fixed, and it only has the freedom of lifting and lowering.

[0028] In this technical solution, the lateral position of the second pipette needle 72 is fixed and only has the freedom to move up and down, while the first pipette needle 71 can not only move up and down but also move laterally along the aforementioned linear displacement direction, thereby achieving the purpose of adjusting and transporting the positions of different reagents or sample liquids in a large area by cooperating with the first pipette needle 71 and the second pipette needle 72.

[0029] See Figure 2 As shown, in some embodiments, the first linear displacement assembly includes a first linear displacement drive assembly (not labeled in the figure), the first linear displacement drive assembly includes a transverse drive motor 7621, an idler wheel 7622 and a synchronous belt 7623, the synchronous belt 7623 is tightened between the output driving wheel of the transverse drive motor 7621 and the idler wheel 7622, ​​and the first pipetting needle 71 is fixedly connected to the synchronous belt 7623. In this technical solution, the lateral position of the first pipetting needle 71 is precisely adjusted by the synchronous belt 7623, the transverse drive motor 7621 and the idler wheel 7622, ​​and the structure is simple.

[0030] As a preferred technical solution, the first linear displacement assembly further includes a linear guide assembly 763, which includes a transverse linear guide rail (not shown in the figure) fixedly connected to the first side surface and a transverse slider (not shown in the figure) fixedly connected to the first pipetting needle 71, and the transverse slider is slidably connected to the transverse linear guide rail, thereby ensuring smooth transverse guidance of the first pipetting needle 71. In another preferred embodiment, the linear guide assembly has two groups spaced apart in an upper and lower manner, further improving the stability and smoothness of the transverse movement of the first pipetting needle 71.

[0031] In some embodiments, a pipette assembly 77 is further assembled on the first side surface, and the suction and discharge capacity of the pipette assembly 77 is smaller than the suction and discharge capacity of the first pipette needle 71 and the second pipette needle 72. The aforementioned pipette assembly 77 can adopt a piston-type pipette structure in the prior art. In this technical solution, the pipette assembly 77 with a smaller suction and discharge capacity can achieve accurate transfer of a smaller volume of reagent.

[0032] In some embodiments, the pipette gun assembly 77 is connected to the first side assembly via a second linear displacement drive assembly 771, the driving direction of the second linear displacement drive assembly 771 is parallel to the driving direction of the first linear displacement drive assembly, and in terms of specific structure, the second linear displacement drive assembly 771 can be the same as the first linear displacement drive assembly.

[0033] In a preferred embodiment, the pipetting gun assembly 77 and the first pipetting needle 71 share the linear guide assembly, so that the structure of the pipetting device is compact while reducing the manufacturing cost of the device.

[0034] According to an embodiment of the utility model, a phage display device is also provided, comprising the above-mentioned liquid transfer device.

[0035] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0036] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred implementations of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A liquid transfer device, characterized in that: The invention comprises a first pipetting needle (71), a second pipetting needle (72), a first delivery pump (73), a second delivery pump (74) and a flow path switching valve (75); the flow path switching valve (75) has a small flow rate pipetting flow path connecting the first delivery pump (73) and the first pipetting needle (71), and a large flow rate pipetting flow path connecting the second delivery pump (74), the first pipetting needle (71) and the second pipetting needle (72); and the flow path switching valve (75) can be controlled to switch between the small flow rate pipetting flow path and the large flow rate pipetting flow path; the pumping flow rate of the second delivery pump (74) is greater than the pumping flow rate of the first delivery pump (73).

2. The liquid transfer device according to claim 1, characterized in that: The flow path switching valve (75) has a first port (751), a second port (752), and a third port (753); the first pipetting needle (71) is connected to the first port (751); the first delivery pump (73) is controllably connected to the second port (752); and the second pipetting needle (72) is controllably connected to the third port (753) via the second delivery pump (74).

3. The liquid transfer device according to claim 1, characterized in that: The first delivery pump (73) is a plunger pump, and the second delivery pump (74) is a peristaltic pump.

4. The liquid transfer device according to claim 1, characterized in that: It also includes a pipetting support (761), the second pipetting needle (72) is assembled on the first side of the pipetting support (761) and can be driven to rise and fall, the first pipetting needle (71) is assembled on the first side via a first linear displacement component and can be driven to rise and fall, and the linear displacement direction of the first linear displacement component is parallel to the horizontal length direction of the pipetting support (761).

5. The liquid transfer device according to claim 4, characterized in that: The first linear displacement component includes a first linear displacement drive component, which includes a transverse drive motor (7621), an idler wheel (7622) and a synchronous belt (7623). The synchronous belt (7623) is tensioned between an output driving wheel of the transverse drive motor (7621) and the idler wheel (7622), and the first pipetting needle (71) is fixedly connected to the synchronous belt (7623).

6. The liquid transfer device according to claim 5, characterized in that: The first linear displacement component also includes a linear guide component (763), which includes a transverse linear guide rail fixedly connected to the first side surface and a transverse sliding block fixedly connected to the first pipetting needle (71), and the transverse sliding block is slidably connected to the transverse linear guide rail.

7. The liquid transfer device according to claim 6, characterized in that: The linear guide assembly comprises two groups which are spaced apart from each other.

8. The liquid transfer device according to claim 6, characterized in that: A pipette gun assembly (77) is also assembled on the first side surface, and the suction and discharge capacity of the pipette gun assembly (77) is smaller than the suction and discharge capacity of the first pipette needle (71) and the second pipette needle (72).

9. The liquid transfer device according to claim 8, characterized in that: The pipette gun assembly (77) is connected to the first side assembly via a second linear displacement drive assembly (771), and the driving direction of the second linear displacement drive assembly (771) is parallel to the driving direction of the first linear displacement drive assembly; and / or the pipette gun assembly (77) and the first pipette needle (71) share the linear guide assembly.

10. A phage display device, characterized in that: A pipetting device comprising any one of claims 1 to 9.

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