Filtering device convenient to clean and used for stem cell separation and extraction

Abstract

The utility model discloses a filtering device convenient to clean for stem cell separation and extraction, and relates to the technical field of stem cells, the device comprises a bottom plate, a filtering barrel is arranged on the bottom plate, the filtering barrel is fixedly connected with the bottom plate through a plurality of buffer mechanisms, a stirring mechanism is arranged in the filtering barrel, and the filtering barrel is fixedly connected with the bottom plate through the buffer mechanisms. A cleaning mechanism is arranged on the barrel wall in the filtering barrel, a collecting box is arranged on one side of the filtering barrel, a filtering mechanism and a buffering mechanism are arranged in the collecting box, so that the shaking amplitude of the filtering barrel in the working process can be reduced, a stirring mechanism works, a diluent and a sample can be uniformly and effectively mixed, and the dilution quality is ensured; the cleaning mechanism can be used for cleaning dirt adhered to the side wall of the filtering barrel, so that the manpower is saved, the working efficiency is improved, and the filtering mechanism is used for filtering and separating the mixed liquid to finish filtering and extracting.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cells, in particular to a filtering device for stem cell separation and extraction which is easy to clean. Background Art

[0002] Stem cells are a type of cell with the potential for self-renewal and differentiation. Different separation methods are required for stem cell extraction depending on the cell type and tissue material. For example, neural stem cells are usually isolated by natural screening using serum-free culture medium, flow cytometry sorting, or immunomagnetic bead sorting. Filtration operations are often required when isolating and extracting stem cells.

[0003] When performing stem cell filtration extraction, the sample must first be diluted evenly and effectively to facilitate subsequent filtration processing. The existing filtration extraction device has a poor dilution effect and is often uneven, which affects the subsequent filtration extraction effect. After long-term use, not only is it easy for excessive dirt to accumulate on the filter net, but dirt will also adhere to the inner wall of the device, which is difficult for staff to clean. The dirt can easily affect the subsequent filtration effect.

[0004] Based on this, a filtration device for stem cell separation and extraction that is easy to clean is now provided, which can eliminate the disadvantages of existing devices. Utility Model Content

[0005] The utility model aims to provide a filtration device for stem cell separation and extraction that is easy to clean, so as to solve the problems that the existing filtration and extraction devices have poor dilution effect and often have uneven dilution effects. Moreover, after long-term use, not only is it easy for excessive dirt to accumulate on the filter net, but dirt will also adhere to the inner wall of the device, which is difficult for staff to clean and the dirt will easily affect the subsequent filtration effect.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A filtering device for stem cell separation and extraction that is easy to clean comprises a bottom plate, a filtering barrel is arranged on the bottom plate, the filtering barrel is fixedly connected to the bottom plate via a plurality of buffer mechanisms, a stirring mechanism is arranged inside the filtering barrel, a cleaning mechanism is arranged on the barrel wall inside the filtering barrel, a collecting box is arranged on one side of the filtering barrel, and a filtering mechanism is arranged inside the collecting box.

[0008] On the basis of the above technical solutions, the present invention also provides the following optional technical solutions:

[0009] In an optional solution: the buffer mechanism includes a fixed block, which is evenly distributed on the outer wall of the filter barrel, a fixed column is arranged through the fixed block, a limiting block is arranged on the top of the fixed column, a base is arranged at the bottom of the fixed column and is fixedly connected to the bottom plate, and a spring is arranged on the outside of the fixed column between the fixed block and the base.

[0010] In an optional scheme: the stirring mechanism includes a rotating rod, a connecting block is provided at the bottom end of the rotating rod, and the connecting block is rotatably connected to the rotating rod, the connecting block is fixedly connected to the inner wall of the filter barrel through a plurality of connecting rods, a plurality of stirring blades are evenly distributed on the rotating rod, a first gear is provided at the top end of the rotating rod penetrating the top end of the filter barrel, a first motor is provided at the top end of the filter barrel on one side of the first gear, a first connecting shaft is provided at the output end of the first motor, a second gear is provided at the top end of the first connecting shaft, and the second gear is meshed with the first gear.

[0011] In an optional scheme: the cleaning mechanism includes a sleeve, which is located on the outside of the rotating rod and is rotatably connected. A fourth gear is provided at the top of the sleeve and passes through the filter barrel. A second motor is provided on one side of the fourth gear. A second connecting shaft is provided at the output end of the second motor. A third gear is provided at the top of the second connecting shaft, and the third gear and the fourth gear are meshed with each other.

[0012] In an optional solution: a feed port is provided at the top end of the filter barrel, a valve is provided at the bottom end of the filter barrel, and a first handle is provided on the valve.

[0013] In an optional solution: a feed pipe is provided on one side of the bottom of the filter barrel, the other end of the feed pipe is connected to the top of the collection box, a water pump is provided on the feed pipe, and a discharge port is provided at the bottom of the collection box.

[0014] In an optional scheme: a fixed plate is provided in the collection box, a limiting plate is provided on one side of the fixed plate and located on the inner wall of the collection box, the limiting plate and the top of the fixed plate are at the same height, a filter plate is provided on the top of the fixed plate, and one end of the filter plate is rotatably connected to the inner wall of the collection box, the other end of the filter plate is fixedly connected to the output end of the third motor on the outer wall of the collection box, the other end of the filter plate is in contact with the limiting plate, and a buffer pad is provided on the inner wall of the collection box away from the limiting plate.

[0015] In an optional solution: a box door is provided on the bottom side of the collection box away from the limiting plate, and a second handle is provided on the box door.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0017] The utility model has a stirring mechanism arranged in the filter barrel, a cleaning mechanism arranged on the barrel wall in the filter barrel, a collecting box arranged on one side of the filter barrel, and a filtering mechanism arranged in the collecting box. The stirring mechanism can make the diluent and the sample be mixed evenly and effectively, thereby ensuring the quality of the dilution and facilitating the subsequent filtering and extraction. The cleaning mechanism can clean the dirt adhered to the side wall of the filter barrel, thereby saving manpower and improving work efficiency. The filtering mechanism can filter and separate the mixed liquid to complete the filtering and extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0019] Figure 2 It is a schematic diagram of the internal overall structure of the utility model.

[0020] Figure 3 This is a schematic diagram of the internal structure of the filter barrel of the utility model

[0021] Figure 4 It is a schematic diagram of the cleaning mechanism structure of the utility model.

[0022] Notes on the figure numbers: 1. bottom plate; 2. filter barrel; 3. fixed block; 4. fixed column; 5. limit block; 6. spring; 7. base; 8. rotating rod; 9. stirring blade; 10. sleeve; 11. fixed rod; 12. brush; 13. feed port; 14. connecting block; 15. connecting rod; 16. valve; 17. first handle; 18. first gear; 19. first motor; 20. first connecting shaft; 21. second gear; 22. second motor; 23. second connecting shaft; 24. third gear; 25. fourth gear; 26. collecting box; 27. feed pipe; 28. water pump; 29. ​​fixed plate; 30. filter plate; 31. limit plate; 32. buffer pad; 33. third motor; 34. discharge port; 35. box door; 36. second handle. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] Example 1

[0025] In one embodiment, Figure 1-Figure 4As shown, a filtering device for stem cell separation and extraction that is easy to clean includes a bottom plate 1, a filtering barrel 2 is arranged on the bottom plate 1, the filtering barrel 2 is fixedly connected to the bottom plate 1 through a plurality of buffer mechanisms, a stirring mechanism is arranged inside the filtering barrel 2, a cleaning mechanism is arranged on the barrel wall inside the filtering barrel 2, a collecting box 26 is arranged on one side of the filtering barrel 2, and a filtering mechanism is arranged inside the collecting box 26. The buffer mechanism can reduce the shaking amplitude of the filtering barrel 2 during the working process, the stirring mechanism can stir and mix the diluent in the filtering barrel 2 with the sample, the cleaning mechanism can clean the dirt adhered to the inner wall of the filtering barrel 2 to prevent the dirt from affecting the subsequent filtering effect, and the filtering mechanism can filter and separate the mixed liquid.

[0026] In one embodiment, Figure 1 As shown, the buffer mechanism includes a fixed block 3, which is evenly distributed on the outer wall of the filter barrel 2. A fixed column 4 is arranged through the fixed block 3, a limiting block 5 is arranged on the top of the fixed column 4, a base 7 is arranged at the bottom of the fixed column 4 and is fixedly connected to the bottom plate 1, and a spring 6 is arranged on the outside of the fixed column 4 between the fixed block 3 and the base 7. The filter barrel 2 reduces the vibration amplitude by compressing the spring 6 up and down, so that the stirring process is more stable.

[0027] In one embodiment, Figure 2 As shown, the stirring mechanism includes a rotating rod 8, a connecting block 14 is provided at the bottom end of the rotating rod 8, and the connecting block 14 is rotatably connected to the rotating rod 8, the connecting block 14 is fixedly connected to the inner wall of the filter barrel 2 through a plurality of connecting rods 15, and a plurality of stirring blades 9 are evenly distributed on the rotating rod 8. A first gear 18 is provided at the top end of the rotating rod 8 penetrating through the top end of the filter barrel 2, and a first motor 19 is provided at the top end of the filter barrel 2 on one side of the first gear 18. A first connecting shaft 20 is provided at the output end of the first motor 19, and a second gear 21 is provided at the top end of the first connecting shaft 20, and the second gear 21 is meshed with the first gear 18. By starting the first motor 19, the rotating rod 8 is driven to rotate, so that the stirring blades 9 on the rotating rod 8 can evenly and effectively mix the diluent and the sample to ensure the quality of the dilution.

[0028] In one embodiment, Figure 2 As shown, the cleaning mechanism includes a sleeve 10, which is located on the outside of the rotating rod 8 and is rotatably connected. A fourth gear 25 is provided at the top of the sleeve 10 that passes through the filter barrel 2. A second motor 22 is provided on one side of the fourth gear 25. A second connecting shaft 23 is provided at the output end of the second motor 22. A third gear 24 is provided at the top of the second connecting shaft 23, and the third gear 24 is meshed with the fourth gear 25. By starting the second motor 22, the sleeve 10 is driven to rotate, so that the brushes 12 on both sides of the sleeve 10 clean the inner wall of the filter barrel 2.

[0029] In one embodiment, Figure 3As shown, a feed port 13 is provided at the top of the filter barrel 2, a valve 16 is provided at the bottom of the filter barrel 2, and a first handle 17 is provided on the valve 16. The diluent and the sample are poured into the filter barrel 2 through the feed port 13, and the valve 16 can be opened to pour out the cleaned impurities inside the filter barrel 2.

[0030] In one embodiment, Figure 2 As shown, a feed pipe 27 is provided on one side of the bottom of the filter barrel 2, and the other end of the feed pipe 27 is connected to the top of the collecting box 26. A water pump 28 is provided on the feed pipe 27. A discharge port 34 is provided at the bottom of the collecting box 26. The diluent and the sample are mixed by the water pump 28 to obtain a mixed liquid, which is injected into the collecting box 26. The filtered and separated liquid falls to the bottom through the filter plate 30 and is discharged through the discharge port 34.

[0031] In one embodiment, Figure 2 As shown, a fixed plate 29 is provided in the collecting box 26, and a limiting plate 31 is provided on one side of the fixed plate 29 on the inner wall of the collecting box 26, and the limiting plate 31 is at the same height as the top of the fixed plate 29, and a filter plate 30 is provided on the top of the fixed plate 29, and one end of the filter plate 30 is rotatably connected to the inner wall of the collecting box 26, and the other end of the filter plate 30 is fixedly connected to the output end of the third motor 33 on the outer wall of the collecting box 26, and the other end of the filter plate 30 is in contact with the limiting plate 31, and a buffer pad 32 is provided on the inner wall of the collecting box 26 away from the limiting plate 31, and the third motor 33 can drive the filter plate 30 to flip, flip the filter plate 30 to one side and hit and vibrate it with the buffer pad 32, so that the residual liquid on the filter plate 30 falls into the cavity on one side of the fixed plate 29 for centralized treatment.

[0032] Example 2

[0033] In one embodiment, as shown in the figure, a box door 35 is provided on the bottom side of the collection box 26 away from the limiting plate 31, and a second handle 36 is provided on the box door 35. The waste liquid can be cleaned by opening the box door 35.

[0034] The above embodiment discloses a filtration device for stem cell separation and extraction that is easy to clean. When in use, the diluent and the sample are poured into the filter barrel 2 through the feed port 13, and the first motor 19 is started to drive the rotating rod 8 to rotate, so that the stirring blades 9 on the rotating rod 8 evenly and effectively mix the diluent and the sample to ensure the quality of the dilution. After the stirring is completed, the diluent and the sample are mixed by the water pump 28 to obtain a mixed liquid that is injected into the collection box 26. The filtered and separated liquid falls to the bottom through the filter plate 30, and the second motor 22 is started to drive the sleeve 10 to rotate, so that the brushes 12 on both sides of the sleeve 10 clean the inner wall of the filter barrel 2. The valve 16 is opened to pour out the cleaned impurities inside the filter barrel 2 and discharge them through the discharge port 34. The third motor 33 is started to drive the filter plate 30 to flip, and the filter plate 30 is flipped to one side to hit and vibrate with the buffer pad 32, so that the residual liquid on the filter plate 30 falls into the cavity on one side of the fixed plate 29 for centralized treatment.

[0035] The above is only a specific implementation of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any person skilled in the art who is familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, which should be included in the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be based on the protection scope of the claims.

Claims

1. A filtration device for stem cell separation and extraction that is easy to clean, comprising a bottom plate (1), on which a filter barrel (2) is arranged, characterized in that: The filter barrel (2) is fixedly connected to the bottom plate (1) via a plurality of buffer mechanisms; a stirring mechanism is provided in the filter barrel (2); a cleaning mechanism is provided on the barrel wall in the filter barrel (2); a collecting box (26) is provided on one side of the filter barrel (2); and a filtering mechanism is provided in the collecting box (26).

2. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: The buffer mechanism comprises a fixed block (3), the fixed block (3) being evenly distributed on the outer wall of the filter barrel (2), a fixed column (4) penetrating the fixed block (3), a limit block (5) being arranged at the top of the fixed column (4), a base (7) being arranged at the bottom of the fixed column (4) and fixedly connected to the bottom plate (1), and a spring (6) being arranged on the outside of the fixed column (4) and between the fixed block (3) and the base (7).

3. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: The stirring mechanism comprises a rotating rod (8), a connecting block (14) is arranged at the bottom end of the rotating rod (8), and the connecting block (14) is rotatably connected to the rotating rod (8), the connecting block (14) is fixedly connected to the inner wall of the filter barrel (2) through a plurality of connecting rods (15), a plurality of stirring blades (9) are evenly distributed on the rotating rod (8), a first gear (18) is arranged at the top end of the rotating rod (8) penetrating the top end of the filter barrel (2), a first motor (19) is arranged at the top end of the filter barrel (2) on one side of the first gear (18), a first connecting shaft (20) is arranged at the output end of the first motor (19), a second gear (21) is arranged at the top end of the first connecting shaft (20), and the second gear (21) and the first gear (18) are meshed with each other.

4. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: The cleaning mechanism comprises a sleeve (10), the sleeve (10) being rotatably connected to the outer side of the rotating rod (8), the top end of the sleeve (10) penetrating the filter barrel (2) and provided with a fourth gear (25), a second motor (22) being provided on one side of the fourth gear (25), a second connecting shaft (23) being provided at the output end of the second motor (22), a third gear (24) being provided at the top end of the second connecting shaft (23), and the third gear (24) and the fourth gear (25) being meshed with each other.

5. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: The top end of the filter barrel (2) is provided with a feed port (13), the bottom end of the filter barrel (2) is provided with a valve (16), and the valve (16) is provided with a first handle (17).

6. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: A feed pipe (27) is provided at one side of the bottom of the filter barrel (2), the other end of the feed pipe (27) is connected to the top of the collection box (26), a water pump (28) is provided on the feed pipe (27), and a discharge port (34) is provided at the bottom of the collection box (26).

7. The easy-to-clean filter device for stem cell separation and extraction according to claim 1, characterized in that: A fixing plate (29) is arranged in the collection box (26); a limiting plate (31) is arranged on one side of the fixing plate (29) and is located on the inner wall of the collection box (26); the limiting plate (31) and the top of the fixing plate (29) are at the same height; a filter plate (30) is arranged on the top of the fixing plate (29); one end of the filter plate (30) is rotatably connected to the inner wall of the collection box (26); the other end of the filter plate (30) is fixedly connected to the output end of a third motor (33) on the outer wall of the collection box (26); the other end of the filter plate (30) is in contact with the limiting plate (31); and a buffer pad (32) is arranged on the inner wall of the collection box (26) away from the limiting plate (31).

8. The easy-to-clean filter device for stem cell separation and extraction according to claim 7, characterized in that: A box door (35) is provided on the bottom of the collection box (26) at a side away from the limiting plate (31), and a second handle (36) is provided on the box door (35).

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