Grape downy mildew in-vitro leaf inoculation culture device

Abstract

The utility model relates to the technical field of leaf culture devices, and discloses a plasmopara viticola in-vitro leaf inoculation culture device, which is characterized in that a slot is formed in the side surface, an end plate is connected in the slot in a sliding manner, a placing plate is arranged on one surface, facing a box body, of the end plate, and the placing plate is connected with the box body in a sliding manner; a lifting plate is slidably connected to the upper end in the box body, light supplementing lamps are evenly arranged on the lower end face of the lifting plate, an electric push rod is arranged on the upper end face of the box body, and a piston rod of the electric push rod is slidably connected with the upper end face of the box body and connected with the lifting plate. A positioning plate is arranged above the open groove in the side face of the box body, a sampling barrel is connected to the positioning plate in a sliding mode, a sampling barrel driving assembly is arranged on the positioning plate, and the sampling barrel driving assembly drives the sampling barrel to move up and down in a reciprocating mode; a material pushing assembly is arranged on the sampling barrel and is used for pushing out a blade sample in the sampling barrel. The device has the advantages of less manual operation, high efficiency and convenience in leaf culture.

Description

Technical Field

[0001] The utility model relates to the technical field of leaf culture devices, in particular to an in vitro leaf inoculation and culture device for grape downy mildew pathogens. Background Art

[0002] Grape downy mildew is the number one disease in grape production, causing huge yield losses every year. Grape downy mildew mainly harms leaves, but can also harm young and tender tissues such as tender shoots, tendrils, petioles, inflorescences, spike axes, fruit stalks and young fruits. In the early stage of the disease, water-soaked yellow spots will appear on the front of the leaves, which will slowly expand into yellow to brown spots. A white mold layer will form on the back of the leaves, which will gradually turn brown in the later stage. Multiple spots can expand and connect into large irregular spots, and the leaves will fall off early in the later stage. The disease can cause leaves to scorch and fall early, new shoots to grow poorly, fruit yield to decrease, quality to deteriorate, and the plant to have poor cold resistance, which seriously affects the yield in the second year. The economic losses caused are huge, and the healthy development of the grape industry is seriously threatened.

[0003] Grape downy mildew is an obligate parasite that can only grow and reproduce on grape leaves. In order to identify grape downy mildew resistance and conduct breeding research, it is necessary to continuously culture the pathogen on grape leaves. The existing in vitro leaf inoculation culture method is to manually cut the leaves, place the cut leaves in a culture dish, and then place the culture dish under appropriate temperature and light conditions for cultivation. It has the following disadvantages:

[0004] 1. The operation requires more manual operation and the efficiency needs to be improved;

[0005] 2. The effect of in vitro leaf inoculation culture is difficult to be effectively guaranteed. Utility Model Content

[0006] 1. Technical Problems Solved

[0007] The technical problem to be solved by the utility model is that the existing in vitro leaf inoculation and culture method requires more manual operations, has low efficiency, and is difficult to ensure the culture effect.

[0008] 2. Technical Solution

[0009] In order to solve the above technical problems, the utility model provides the following technical solutions: a device for inoculating and cultivating grape downy mildew bacteria in vitro on leaves, comprising a box body, wherein support blocks are provided at the four corners of the lower end surface of the box body.

[0010] The side of the box body is provided with a groove, and an end plate is slidably connected in the groove. A placement plate is provided on the side of the end plate facing the box body, and the placement plate is slidably connected to the box body. A lifting plate is slidably connected to the upper end of the box body, and fill lights are evenly arranged on the lower end surface of the lifting plate. An electric push rod is provided on the upper end surface of the box body, and the piston rod of the electric push rod is slidably connected to the upper end surface of the box body and connected to the lifting plate;

[0011] A positioning plate is provided above the slot on the side of the box body, a sampling barrel is slidably connected to the positioning plate, a sampling barrel driving assembly is provided on the positioning plate, and the sampling barrel driving assembly drives the sampling barrel to reciprocate up and down;

[0012] The sampling barrel is provided with a pushing assembly, and the pushing assembly pushes out the leaf sample in the sampling barrel.

[0013] Furthermore, the sampling tube drive assembly includes a connecting plate and an electromagnet. A connecting plate is provided on the upper end of the side of the sampling tube, a guide rod is provided on the upper end surface of the positioning plate, the connecting plate is slidably connected to the guide rod, a spring is provided between the connecting plate and the positioning plate, and an electromagnet is also provided on the positioning plate, and the electromagnet is located below one end of the connecting plate.

[0014] Furthermore, the spring set is arranged on the outside of the guide rod.

[0015] Furthermore, the pushing assembly includes electromagnet 2 and a pushing plate. Electromagnet 2 is provided on the side of the sampling barrel. A pushing plate is slidably connected inside the sampling barrel. A connecting rod is provided on the upper end surface of the pushing plate. The connecting rod is slidably connected to the upper end of the sampling barrel. A limiting ring is provided on the portion of the connecting rod exposed from the sampling barrel. A spring 2 is provided between the limiting ring and the upper end surface of the sampling barrel. The spring 2 is sleeved on the outside of the connecting rod. A transmission plate is provided above the electromagnet 2. The transmission plate is connected to the upper end of the connecting rod through a bent rod.

[0016] Furthermore, a handle is provided on a side of the end plate away from the box body.

[0017] Furthermore, the box body is provided with a controller, and the controller is provided with control buttons.

[0018] 3. Beneficial Effects

[0019] The advantages of the utility model compared with the prior art are:

[0020] The sampling tube driving assembly drives the sampler to reciprocate up and down, and the sampling tube samples the leaves. After the sampling is completed, the pushing assembly pushes the sampled leaves in the sampling tube into the culture dish, places the culture dish on the placement plate, and pushes the placement plate into the box. The fill light on the lifting plate in the box controls the temperature and light in the box, so that the device has less manual operation and high processing efficiency, while improving the cultivation effect of the leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the utility model.

[0022] Figure 2 It is a schematic diagram of the connection between the positioning plate and the sampling tube of the utility model.

[0023] Figure 3 It is a cross-sectional view of the box body of the utility model.

[0024] Figure 4 It is a cross-sectional view of the sampling tube of the utility model.

[0025] Figure numerals: 1, box body, 2, support block, 3, slot, 4, end plate, 5, placement plate, 6, lifting plate, 7, fill light, 8, electric push rod, 9, positioning plate, 10, sampling tube, 11, connecting plate, 12, electromagnet 1, 13, guide rod, 14, spring 1, 15, electromagnet 2, 16, push plate, 17, connecting rod, 18, limiting ring, 19, spring 2, 20, transmission plate, 21, bending rod, 22, handle, 23, controller, 24, control button. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present utility model are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present utility model, not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present utility model.

[0027] It should be noted that all directional indications such as up, down, left, right, front, back... in the embodiments of the utility model are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture as shown in the accompanying drawings. If the specific posture changes, the directional indication will also change accordingly.

[0028] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0029] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0030] The utility model will now be further described in conjunction with the accompanying drawings.

[0031] Embodiment 1

[0032] Combined with Figure 1-2 A positioning plate 9 is provided above the slot 3 on the side of the box body 1, and a sampling cylinder 10 is slidably connected to the positioning plate 9. A sampling cylinder driving assembly is provided on the positioning plate 9, and the sampling cylinder driving assembly drives the sampling cylinder 10 to reciprocate up and down. The sampling cylinder driving assembly includes a connecting plate 11 and an electromagnet 12. A connecting plate 11 is provided on the upper end of the side of the sampling cylinder 10, and a guide rod 13 is provided on the upper end surface of the positioning plate 9. The connecting plate 11 is slidably connected to the guide rod 13, and a spring 14 is provided between the connecting plate 11 and the positioning plate 9. An electromagnet 12 is also provided on the positioning plate 9, and the electromagnet 12 is located below one end of the connecting plate 11.

[0033] When in use, the blade to be sampled is placed under the sampling tube 10, and the electromagnet 12 is energized to magnetically attract the connecting plate 11, which drives the sampling tube 10 to move downward, and the sampling tube 10 samples the blade of a fixed size. After the sampling is completed, the electromagnet 12 is de-energized, and the sampling tube 10 returns to its initial position under the action of the spring 14.

[0034] Combined with Figure 2 and 4 A pushing assembly is provided on the sampling barrel 10, and the pushing assembly pushes out the blade sample in the sampling barrel 10. The pushing assembly includes an electromagnet 15 and a pushing plate 16. An electromagnet 15 is provided on the side of the sampling barrel 10. A pushing plate 16 is slidably connected in the sampling barrel 10. A connecting rod 17 is provided on the upper end surface of the pushing plate 16. The connecting rod 17 is slidably connected to the upper end of the sampling barrel 10. A limiting ring 18 is provided on the portion of the connecting rod 17 exposed from the sampling barrel 10. A spring 19 is provided between the limiting ring 18 and the upper end surface of the sampling barrel 10. The spring 19 is sleeved on the outside of the connecting rod 17. A transmission plate 20 is provided above the electromagnet 15, and the transmission plate 20 is connected to the upper end of the connecting rod 17 through a bent rod 21.

[0035] After the sampling is completed, the electromagnet 15 is energized to magnetically attract the transmission plate 20, and the transmission plate 20 drives the push plate 16 to move downward in the sampling tube 10 through the bent rod 21 and the connecting rod 17, pushing out the leaves in the sampling tube 10 and making them fall into the culture dish, realizing automatic discharge. After the discharge is completed, under the action of the limit ring 18 and the spring 19, the push plate 16 returns to the initial position.

[0036] Combined with Figure 1 and 3 The side surface is provided with a groove 3, and an end plate 4 is slidably connected in the groove 3. A placement plate 5 is provided on the side of the end plate 4 facing the box body 1, and the placement plate 5 is slidably connected to the box body 1. A lifting plate 6 is slidably connected to the upper end of the box body 1, and fill lights 7 are evenly arranged on the lower end surface of the lifting plate 6. A handle 22 is provided on the side of the end plate 4 away from the box body 1.

[0037] The culture dish is placed on the placement plate 5, and the placement plate 5 is pushed into the box body 1 through the handle 22 and the end plate 4. The fill light 7 on the lifting plate 6 in the box body 1 controls the temperature and light to ensure effective culture.

[0038] Embodiment 2

[0039] Combined with Figure 1 and 3 An electric push rod 8 is provided on the upper end surface of the box body 1 , and a piston rod of the electric push rod 8 is slidably connected to the upper end surface of the box body 1 and is connected to the lifting plate 6 .

[0040] On the basis of the first embodiment, the piston rod of the electric push rod 8 is extended and retracted to adjust the height of the lifting plate 6, thereby adjusting the distance between the fill light 7 and the culture dish, and further finely adjusting the temperature and light.

[0041] The utility model and its implementation are described, and such description is not restrictive. The drawings are only one of the implementations of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A device for inoculating and cultivating grape downy mildew bacteria in vitro from leaves, comprising a box (1), wherein support blocks (2) are provided at the four corners of the lower end surface of the box (1), and the device is characterized in that: The side of the box body (1) is provided with a groove (3), an end plate (4) is slidably connected in the groove (3), a placement plate (5) is provided on the side of the end plate (4) facing the box body (1), the placement plate (5) is slidably connected to the box body (1), a lifting plate (6) is slidably connected to the upper end of the box body (1), a fill light (7) is evenly arranged on the lower end surface of the lifting plate (6), an electric push rod (8) is provided on the upper end surface of the box body (1), and the piston rod of the electric push rod (8) is slidably connected to the upper end surface of the box body (1) and connected to the lifting plate (6); A positioning plate (9) is provided above the side slot (3) of the box body (1), a sampling tube (10) is slidably connected to the positioning plate (9), and a sampling tube driving assembly is provided on the positioning plate (9), and the sampling tube driving assembly drives the sampling tube (10) to reciprocate up and down; The sampling cylinder (10) is provided with a pushing assembly, which pushes the leaf sample in the sampling cylinder (10) out.

2. The in vitro leaf inoculation and cultivation device for grape downy mildew according to claim 1, characterized in that: The sampling tube driving assembly comprises a connecting plate (11) and an electromagnet (12). The upper end of the side of the sampling tube (10) is provided with a connecting plate (11). The upper end surface of the positioning plate (9) is provided with a guide rod (13). The connecting plate (11) is slidably connected to the guide rod (13). A spring (14) is provided between the connecting plate (11) and the positioning plate (9). The positioning plate (9) is also provided with an electromagnet (12). The electromagnet (12) is located below one end of the connecting plate (11).

3. The in vitro leaf inoculation and cultivation device for grape downy mildew according to claim 2, characterized in that: The spring 1 (14) is sleeved on the outside of the guide rod (13).

4. The in vitro leaf inoculation and cultivation device for grape downy mildew according to claim 1, characterized in that: The push assembly comprises an electromagnet (15) and a push plate (16). The side of the sampling barrel (10) is provided with an electromagnet (15). The sampling barrel (10) is slidably connected with a push plate (16). The upper end surface of the push plate (16) is provided with a connecting rod (17). The connecting rod (17) is slidably connected to the upper end of the sampling barrel (10). A limiting ring (18) is provided on the portion of the connecting rod (17) exposed from the sampling barrel (10). A spring (19) is provided between the limiting ring (18) and the upper end surface of the sampling barrel (10). The spring (19) is sleeved on the outer side of the connecting rod (17). A transmission plate (20) is provided above the electromagnet (15). The transmission plate (20) is connected to the upper end of the connecting rod (17) through a bent rod (21).

5. The in vitro leaf inoculation and cultivation device for grape downy mildew according to claim 1, characterized in that: A handle (22) is provided on a surface of the end plate (4) away from the box body (1).

6. The in vitro leaf inoculation and cultivation device for grape downy mildew according to claim 1, characterized in that: The box body (1) is provided with a controller (23), and the controller (23) is provided with a control button (24).

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