Escherichia coli cracking device
By designing an E. coli lysis device including a base, a scaffold, a adjustment screw and a stirring member, the problem of inconvenience of manually shaking the test tube is solved, and efficient stirring of the E. coli solution is achieved, reducing the operating time and improving efficiency.
Patent Information
- Application Number
- CN202420556272.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-21
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-03-21
AI Technical Summary
The prior art is inconvenient to manually shake when handling large quantities of test tubes, which are inefficient and time-consuming.
An E. coli lysis device is designed, including a base, a scaffold, a adjustment screw, agitator and a carrier plate. By adjusting screw, the stirring member is driven downward to achieve stirring and mixing of the E. coli solution in multiple test tubes.
The device can efficiently stir and mix E. coli solution in multiple test tubes, significantly reduce the operating time of the operator, improve the efficiency of test tube mixing, and fix the test tube through the limiting parts to prevent the solution from leaking.
Smart Images
Figure CN222613345U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of Escherichia coli lysis devices. Background Technology
[0002] The primary method for obtaining plasmids from engineered E. coli bacteria is alkaline lysis. The principle of alkaline lysis is that a high-pH solution causes cell wall residue, thus denaturing DNA and proteins. During lysis, bacterial proteins, ruptured cell walls, and denatured chromosomal DNA intertwine to form large complexes, which are then coated with dodecyl sulfate. When Na+ is replaced with K+, the complexes precipitate effectively from the solution. After centrifugation to remove the denaturing agent, the renatured plasmid DNA can be recovered from the supernatant.
[0003] Escherichia coli is an engineered strain widely used in E. coli lysis devices. When using E. coli as a host cell to induce recombinant protein expression or to extract E. coli DNA, it is necessary to lyse the E. coli to break its outer wall and expose the protein. In the laboratory, E. coli and lysis buffer are usually mixed in test tubes and then shaken or inverted to ensure homogeneity. However, this method is no longer suitable when mixing a large number of test tubes. Therefore, an E. coli lysis device is needed that can simultaneously mix test tubes to improve mixing efficiency and reduce operator time. Utility Model Content
[0004] The purpose of this invention is to provide an Escherichia coli lysis device that overcomes the inconvenience of manually shaking a large number of test tubes.
[0005] The technical solution to achieve the above objective is: an Escherichia coli lysis device, comprising a base, a support connected to the top of the base, an adjusting screw threaded to the upper end of the support, a stirring element rotatably connected to the bottom end of the adjusting screw, a carrier plate connected to the top of the base, a plurality of placement slots opened at the top of the carrier plate, test tubes inserted into the inner wall of the placement slots, and a limiting element provided on the outer wall of the support.
[0006] Preferably, the top end of the adjusting screw is connected to a handle.
[0007] Preferably, the stirring component includes an outer box, a motor, a rotating shaft, a drive gear, a connecting gear, and a stirring roller. The bottom end of the adjusting screw is rotatably connected to the outer box, the bottom end of the outer box is rotatably connected to the rotating shaft, the bottom end of the rotating shaft is connected to the stirring roller, the upper end of the outer wall of the rotating shaft is connected to the drive gear, and the bottom end of the inner wall of the outer box is rotatably connected to multiple connecting gears. The outer walls of the connecting gears mesh with the drive gears, and the outer wall of the outer box is slidably connected to the inner wall of the support. The top end of one of the rotating shafts is connected to the motor, and the outer wall of the motor is connected to the outer box.
[0008] Preferably, a guide rod is connected to the top of the outer box, the outer wall of the guide rod is slidably connected to the top of the bracket, and the placement groove is located directly below the stirring roller.
[0009] Preferably, the limiting component includes a through hole, a connecting rod, a screw ring, a pressure plate, and a support screw. The outer wall of the bracket has two symmetrically distributed through holes. The inner wall of the through holes is slidably connected to two symmetrically distributed connecting rods. The outer wall of the bracket is connected to two symmetrically distributed support screws. The outer wall of each support screw is threaded with two screw rings. The outer wall of every two screw rings is rotatably connected to the connecting rod. The outer wall of the connecting rod is connected to a plurality of arrayed pressure plates.
[0010] Preferably, the outer wall of the compression plate is fitted with a rubber pad.
[0011] Preferably, the bottom end of the outer box is connected to a connecting plate, and the bottom end of the connecting plate is connected to a plurality of arrayed cover rings, and the outer wall of the stirring roller penetrates the bottom end of the connecting plate.
[0012] Preferably, the cover ring is located on the upper outer side of the stirring roller, and a sealing ring is fitted at the contact point between the stirring roller and the cover ring.
[0013] The beneficial effects of this utility model are:
[0014] 1) Place multiple test tubes containing E. coli solution vertically into the placement groove, use the limiting device to clamp and fix the test tubes, rotate the adjusting screw to move the stirring device downwards and extend it into the test tubes, thereby achieving the effect of stirring and mixing the E. coli solution in multiple test tubes.
[0015] 2) Rotate the adjusting screw, which moves the outer box downwards, causing the rotating shaft to move downwards. The rotating shaft then moves the stirring roller downwards, positioning the placement slot directly below the stirring roller, thus moving it into the test tube. Start the motor, which drives one of the rotating shafts to rotate. This rotating shaft drives one of the drive gears, which in turn drives the connecting gear, which in turn drives the remaining drive gears, ultimately driving the remaining rotating shafts to rotate. The rotating shafts then drive all the stirring rollers to rotate, achieving the effect of stirring and mixing the E. coli solutions in multiple test tubes. When the outer box moves downwards, it causes the connecting plate to move downwards, which in turn causes the cover ring to move downwards, covering the opening of the test tube and preventing the E. coli solution from leaking out. Attached Figure Description
[0016] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;
[0017] Figure 2 This is a bottom view structural diagram of this utility model;
[0018] Figure 3 This is a cross-sectional structural schematic diagram of the present invention;
[0019] Figure 4 yes Figure 1 Enlarged structural diagram at point A;
[0020] Figure 5 yes Figure 2 Enlarged structural diagram at point B;
[0021] Figure 6 yes Figure 3 A magnified structural diagram at point C.
[0022] 1. Base; 2. Bracket; 3. Adjusting screw; 4. Outer box; 5. Motor; 6. Rotating shaft; 7. Drive gear; 8. Connecting gear; 9. Stirring roller; 10. Carrier plate; 11. Placement groove; 12. Test tube; 13. Through hole; 14. Connecting rod; 15. Threaded ring; 16. Pressing plate; 17. Guide rod; 18. Connecting plate; 19. Cover ring; 20. Rotating handle; 21. Support screw. Detailed Implementation
[0023] The technical solution of this utility model will now be clearly and completely described in conjunction with the accompanying drawings. In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0024] The present invention will be further described below with reference to the accompanying drawings.
[0025] Reference Appendix Figure 1-6 An Escherichia coli lysis device includes a base 1, a support 2 connected to the top of the base 1, an adjusting screw 3 threadedly connected to the upper end of the support 2, a handle 20 connected to the top of the adjusting screw 3, a stirring element rotatably connected to the bottom end of the adjusting screw 3, a carrier plate 10 connected to the top of the base 1, a plurality of placement slots 11 opened at the top of the carrier plate 10, test tubes 12 inserted into the inner wall of the placement slots 11, and a limiting element provided on the outer wall of the support 2.
[0026] Multiple test tubes 12 containing E. coli solution are vertically placed into the placement groove 11. The test tubes 12 are clamped and fixed using the limiting device. The adjusting screw 3 is rotated to move the stirring device downwards and extend it into the test tubes 12, thereby achieving the effect of stirring and mixing the E. coli solution in the multiple test tubes 12.
[0027] Reference Appendix Figure 2-6 The mixing components include an outer box 4, a motor 5, a rotating shaft 6, a drive gear 7, a connecting gear 8, and a mixing roller 9. The bottom end of the adjusting screw 3 is rotatably connected to the outer box 4, and the bottom end of the outer box 4 is rotatably connected to the rotating shaft 6. The bottom end of the rotating shaft 6 is connected to the mixing roller 9. The upper end of the outer wall of the rotating shaft 6 is connected to the drive gear 7, and the bottom end of the inner wall of the outer box 4 is rotatably connected to multiple connecting gears 8. The outer wall of the connecting gears 8 meshes with the drive gear 7. The outer wall of the outer box 4 is slidably connected to the inner wall of the support 2. One of the rotating shafts 6... The top of the device is connected to a motor 5, the outer wall of the motor 5 is connected to the outer box 4, the top of the outer box 4 is connected to a guide rod 17, the outer wall of the guide rod 17 is slidably connected to the top of the bracket 2, the placement groove 11 is located directly below the stirring roller 9, the bottom of the outer box 4 is connected to a connecting plate 18, the bottom of the connecting plate 18 is connected to multiple arrayed cover rings 19, the outer wall of the stirring roller 9 penetrates the bottom of the connecting plate 18, the cover rings 19 are located on the upper outer side of the stirring roller 9, and a sealing ring is fitted at the contact point between the stirring roller 9 and the cover rings 19.
[0028] Rotating the adjusting screw 3 causes the outer box 4 to move downwards, which in turn moves the rotating shaft 6 downwards. The rotating shaft 6 then moves the stirring roller 9 downwards, allowing it to be placed into the test tube 12 by placing the placement slot 11 directly below the stirring roller 9. The motor 5 is then started, causing one of the rotating shafts 6 to rotate. This rotating shaft 6 then drives one of the drive gears 7, which in turn drives the connecting gear 8, which in turn drives the remaining drive gears 7, ultimately driving the remaining rotating shafts 6 to rotate. The rotating shafts 6 then drive all the stirring rollers 9 to rotate, achieving the effect of mixing the E. coli solution in multiple test tubes 12. When the outer box 4 moves downwards, it causes the connecting plate 18 to move downwards, which in turn causes the cover ring 19 to move downwards, covering the opening of the test tube 12 to prevent the E. coli solution from leaking out.
[0029] Reference Appendix Figure 4-5 The limiting component includes a through hole 13, a connecting rod 14, a screw ring 15, a pressure plate 16, and a support screw 21. The outer wall of the bracket 2 has two symmetrically distributed through holes 13. The inner wall of the through holes 13 is slidably connected to two symmetrically distributed connecting rods 14. The outer wall of the bracket 2 is connected to two symmetrically distributed support screws 21. The outer wall of each support screw 21 is threadedly connected to two screw rings 15. The outer wall of every two screw rings 15 is rotatably connected to the connecting rod 14. The outer wall of the connecting rod 14 is connected to multiple arrayed pressure plates 16. The outer wall of the pressure plate 16 is fitted with a rubber pad.
[0030] Multiple test tubes 12 containing E. coli solution are vertically placed into the placement groove 11. The screw ring 15 is rotated, and the screw ring 15 pushes the two connecting rods 14 to move towards each other, so that the pressure plates 16 on both sides can move towards each other to clamp the test tubes 12, thereby fixing the test tubes 12.
[0031] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model.
Claims
1. An Escherichia coli lysis device, comprising a base (1), characterized in that: The top end of the base (1) is connected to a bracket (2), the upper end of the bracket (2) is threadedly connected to an adjusting screw (3), the bottom end of the adjusting screw (3) is rotatably connected to a stirring member, the top end of the base (1) is connected to a carrier plate (10), the top end of the carrier plate (10) is provided with a plurality of placement grooves (11), the inner walls of the placement grooves (11) are connected with test tubes (12), and the outer wall of the bracket (2) is provided with a limiting member.
2. The E. coli lysis device according to claim 1, characterized in that: The top end of the adjusting screw rod (3) is connected with a rotating handle (20).
3. The E. coli lysis device according to claim 1, characterized in that: The stirring member comprises an outer box (4), a motor (5), a rotating shaft (6), a driving gear (7), a connecting gear (8) and a stirring roller (9); the bottom end of the adjusting screw (3) is rotatably connected to the outer box (4); the bottom end of the outer box (4) is rotatably connected to the rotating shaft (6); the bottom end of the rotating shaft (6) is connected to the stirring roller (9); the upper end of the outer wall of the rotating shaft (6) is connected to the driving gear (7); the bottom end of the inner wall of the outer box (4) is rotatably connected to a plurality of connecting gears (8); the outer wall of the connecting gear (8) is meshed with the driving gear (7); the outer wall of the outer box (4) is slidably connected to the inner wall of the bracket (2); the top end of one of the rotating shafts (6) is connected to the motor (5); and the outer wall of the motor (5) is connected to the outer box (4).
4. The E. coli lysis device according to claim 3, characterized in that: The top end of the outer box (4) is connected to a guide rod (17), the outer wall of the guide rod (17) is slidably connected to the top end of the bracket (2), and the placement groove (11) is located directly below the stirring roller (9).
5. The E. coli lysis device according to claim 1, characterized in that: The limiting member comprises a through hole (13), a connecting rod (14), a screw ring (15), a pressing plate (16) and a supporting screw (21); the outer wall of the bracket (2) is provided with two symmetrically distributed through holes (13); the inner wall of the through hole (13) is slidably connected to two symmetrically distributed connecting rods (14); the outer wall of the bracket (2) is connected to two symmetrically distributed supporting screws (21); the outer wall of each supporting screw (21) is threadedly connected to two screw rings (15); the outer walls of every two screw rings (15) are rotatably connected to the connecting rod (14); and the outer wall of the connecting rod (14) is connected to a plurality of pressing plates (16) distributed in an array.
6. The E. coli lysis device according to claim 5, characterized in that: The outer wall of the pressing sheet (16) is sleeved with a rubber pad.
7. The E. coli lysis device according to claim 3, characterized in that: The bottom end of the outer box (4) is connected to a connecting plate (18), the bottom end of the connecting plate (18) is connected to a plurality of cover rings (19) distributed in an array, and the outer wall of the stirring roller (9) passes through the bottom end of the connecting plate (18).
8. The E. coli lysis device according to claim 7, characterized in that: The cover ring (19) is located on the upper outer side of the stirring roller (9), and a sealing ring is sleeved at the contact point between the stirring roller (9) and the cover ring (19).