Dynamic axial compression chromatographic column

Abstract

The utility model discloses a dynamic axial compression chromatographic column which comprises a column tube, a lower cover arranged at the lower end of the column tube, a base connected with the lower cover, a plugging piece and a driving piece used for driving the plugging piece to stretch out and draw back, a homogenate inlet is formed in the side wall of the base, and a homogenate outlet is formed in the top of the base. The homogenate inlet and the homogenate outlet are communicated through a liquid inlet channel arranged in the base, and the upper end of the plugging piece is arranged in the liquid inlet channel. The homogenate inlet, the liquid inlet channel and the homogenate outlet are all integrated on the base, a feeding pipe does not need to be arranged, and the upper end of the plugging piece is arranged in the liquid inlet channel, so that the number of parts can be reduced, the structure is simplified, and flushing and maintenance are convenient.

Description

Technical Field

[0001] The utility model relates to a chromatographic column, in particular to a dynamic axial compression chromatographic column. Background Art

[0002] The existing high-pressure dynamic axial compression chromatography column is to inject the homogenate into the column tube, and then drive the piston of the chromatography column downward through the hydraulic cylinder to press out the mobile phase in the homogenate, so that the filler can be evenly retained in the column tube and compacted. At present, there are two main ways to inject the homogenate: one is to raise the piston above the column tube, pump the homogenate into the chromatography column through the infusion pump, and then press the piston down; the other is to place the piston body in the chromatography column, open a hole in the side wall of the chromatography column below the piston body, and pump the homogenate into the column through the hole. Both methods have major defects: when the first method is used, impurities are easily introduced into the pumped homogenate, while when the second method is used, the dead volume or residue between the holes is easy to deteriorate and mold, and the potential health hazards are obvious, and neither meets the requirements of relevant regulations; after the filler in the homogenate is pumped into the column, it begins to settle immediately due to the action of gravity, and the continuous injection of the homogenate further accelerates the settling of the filler, which will cause the density of the filler column bed after the final filling to be uneven or have a "sugar core", which directly affects the separation and purification effects of the equipment. The production performance of the equipment is naturally greatly reduced, and as the diameter of the chromatographic column increases, this negative force has a greater impact, which is also one of the pain points of this type of equipment.

[0003] Chinese patent document CN 219251720 U discloses a dynamic axial compression chromatographic column without hydraulic cylinder, wherein a homogenate feed valve is provided on the lower cover of the column tube, and the homogenate feed valve can be used to inject the homogenate into the cavity below the piston body from bottom to top, without raising the piston body above the column tube, and without opening holes on the side wall of the column tube, and the downward gravity of the homogenate in the column tube is opposite to the upward impact force of the continuously injected homogenate, thereby offsetting each other, which is conducive to eliminating the problem of uneven density of the packing column bed after the final filling or the appearance of "sugar core". However, the technical solution is to install the feed pipe in the valve seat, and use the telescopic movement of the valve core in the feed pipe to control the on-off of the flow channel. The structure is relatively complex, the assembly is difficult, and the workload of flushing and maintenance is increased. In addition, the pneumatic motor drives the connecting nut to rotate, thereby realizing the telescopic movement of the valve core. When the rotating shaft of the pneumatic motor is not concentric with the valve core, it is easy to get stuck. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a dynamic axial compression chromatographic column with a small number of parts, a simple structure, and convenient flushing and maintenance.

[0005] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0006] A dynamic axial compression chromatographic column comprises a column tube, a lower cover arranged at the lower end of the column tube and a base connected to the lower cover, and also comprises a plugging member and a driving member for driving the plugging member to extend and retract. A homogenization inlet is arranged on the side wall of the base and a homogenization outlet is arranged on the top. The homogenization inlet and the homogenization outlet are connected through a liquid inlet channel arranged inside the base, and the upper end of the plugging member is arranged in the liquid inlet channel.

[0007] As a further improvement of the above technical solution: a sealing cover is provided on the top of the blocking member.

[0008] As a further improvement of the above technical solution: a first sealing ring is provided between the upper end of the blocking member and the base, and a second sealing ring is provided between the lower end of the blocking member and the base.

[0009] As a further improvement of the above technical solution: the diameter of the upper end of the liquid inlet channel is smaller than the diameter of the lower end.

[0010] As a further improvement of the above technical solution: a sieve plate is provided above the lower cover, and the top of the base is flush with the upper surface of the sieve plate.

[0011] As a further improvement of the above technical solution: the base and the lower cover are fixedly connected by fasteners.

[0012] As a further improvement of the above technical solution: the driving member is a telescopic structure.

[0013] As a further improvement of the above technical solution: the driving member is a cylinder, and the piston rod of the cylinder is connected to the lower end of the blocking member.

[0014] As a further improvement of the above technical solution: the lower end of the piston rod is provided with a scale mark and passes through the cylinder body of the cylinder.

[0015] As a further improvement of the above technical solution: a drainage gap is provided between the outer wall of the base and the lower cover, and a drainage interface communicating with the drainage gap is provided on the base.

[0016] Compared with the prior art, the advantages of the utility model are: the dynamic axial compression chromatographic column disclosed by the utility model, the slurry inlet, the liquid inlet channel and the slurry outlet are all integrated on the base, there is no need to set up a feed pipe, and the upper end of the plugging part is arranged in the liquid inlet channel, which can reduce the number of parts, simplify the structure, and facilitate flushing and maintenance.

[0017] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1It is a structural schematic diagram of the dynamic axial compression chromatographic column of the utility model.

[0019] Figure 2 It is a schematic cross-sectional structure diagram of the utility model in which the chromatographic column is dynamically compressed axially into a homogenous liquid state.

[0020] Figure 3 It is a schematic cross-sectional structure diagram of the dynamic axial compression chromatographic column of the utility model in use state.

[0021] Figure 4 It is a schematic cross-sectional structure diagram of the dynamic axial compression chromatographic column of the utility model in a liquid discharge state.

[0022] Figure 5 yes Figure 3 A partial enlarged view of .

[0023] The symbols in the figure represent:

[0024] 1. Column tube; 2. Lower cover; 3. Base; 31. Slurry inlet; 32. Slurry outlet; 33. Liquid inlet channel; 34. Liquid discharge gap; 35. Liquid discharge interface; 4. Blocking member; 41. Sealing cover; 42. First sealing ring; 43. Second sealing ring; 5. Driving member; 51. Piston rod; 52. Cylinder body; 6. Sieve plate. DETAILED DESCRIPTION

[0025] In the description of the present invention, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0026] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0027] In the present invention, unless otherwise clearly specified and limited, the terms "assemble", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] Figures 1 to 5 An embodiment of the dynamic axial compression chromatographic column of the utility model is shown. The dynamic axial compression chromatographic column of the embodiment includes a column tube 1, a lower cover 2 arranged at the lower end of the column tube 1, and a base 3 connected to the lower cover 2, and also includes a plugging member 4 and a driving member 5 for driving the plugging member 4 to extend and retract. A homogenization inlet 31 is provided on the side wall of the base 3 and a homogenization outlet 32 ​​is provided on the top. The homogenization inlet 31 and the homogenization outlet 32 ​​are connected through a liquid inlet channel 33 arranged inside the base 3, and the upper end of the plugging member 4 is arranged in the liquid inlet channel 33. Preferably, two homogenization inlets 31 are provided and arranged on both sides of the base 3 opposite to each other.

[0030] In the dynamic axial compression chromatography column of this embodiment, the slurry inlet 31, the liquid inlet channel 33 and the slurry outlet 32 ​​are all integrated on the base 3, and there is no need to set a feed pipe. The upper end of the sealing member 4 is arranged in the liquid inlet channel 33, which can reduce the number of parts, simplify the structure, and facilitate flushing and maintenance.

[0031] Furthermore, in this embodiment, a sealing cover 41 is provided on the top of the plugging member 4. When the homogenate outlet 32 ​​needs to be blocked, the plugging member 4 moves upward into place under the action of the driving member 5, and the sealing cover 41 is pressed tightly, which is conducive to ensuring the sealing of the homogenate outlet 32, and the structure is simple and effective.

[0032] Furthermore, in this embodiment, a first sealing ring 42 is provided between the upper end of the plugging member 4 and the base 3, and a second sealing ring 43 is provided between the lower end of the plugging member 4 and the base 3. The plugging member 4 moves upward into position under the action of the driving member 5, and the sealing cover 41 is pressed, thereby sealing the homogenate outlet 32. At the same time, the first sealing ring 42 can form a second-level seal between the upper end of the plugging member 4 and the base 3, which can effectively separate the homogenate inlet 31 and the homogenate outlet 32; the second sealing ring 43 can form a third-level seal between the lower end of the plugging member 4 and the base 3 to prevent the homogenate liquid from leaking from below the liquid inlet channel 33. The structure is reasonable and effective.

[0033] As a preferred embodiment, the diameter of the upper end of the liquid inlet channel 33 is smaller than the diameter of the lower end. When the upper end of the plugging member 4 is located at the lower end of the liquid inlet channel 33, there is a gap between the two, so that the homogenization inlet 31 is connected with the homogenization outlet 32; when the upper end of the plugging member 4 moves to the upper end of the liquid inlet channel 33, a good seal can be formed between the two through the first sealing ring 42, effectively isolating the homogenization inlet 31 and the homogenization outlet 32, and the structure is simple and reasonable.

[0034] As a preferred embodiment, a sieve plate 6 is provided above the lower cover 2, and the top of the base 3 is flush with the upper surface of the sieve plate 6, so that the bottom of the column tube 1 can be kept flat.

[0035] As a preferred embodiment, the base 3 and the lower cover 2 are fixedly connected by fasteners (only threaded holes are shown in the figure, and the fasteners can be bolts, screws, etc.), which can achieve reliable connection between the base 3 and the lower cover 2 and facilitate installation and removal. Correspondingly, the lower cover 2 and the column tube 1, and the cylinder body 52 of the cylinder and the frame can also be fixedly connected by fasteners.

[0036] As a preferred embodiment, the driving member 5 is a telescopic structure. Compared with the existing pneumatic motor combined with the rotation of the nut to drive the valve core to extend and retract, the telescopic driving member 5 is directly used to drive the blocking member 4 to move up and down, which has a simpler structure and is more convenient to install. At the same time, it avoids the problem of jamming when the rotating shaft and the valve core are not concentric.

[0037] Furthermore, in this embodiment, the driving member 5 is a cylinder, and the piston rod 51 of the cylinder is connected to the lower end of the blocking member 4. The cylinder technology is mature and has good reliability. Of course, in other embodiments, it can also be an oil cylinder.

[0038] Furthermore, in this embodiment, a scale mark (not shown) is provided at the lower end of the piston rod 51 and passes through the cylinder body 52 of the cylinder. The operator can visually observe the scale mark exposed at the lower end of the piston rod 51, and then judge whether the blocking member 4 is raised or lowered into place, further improving the reliability and convenience of the equipment.

[0039] Furthermore, in this embodiment, there is a drainage gap 34 between the outer wall of the base 3 and the lower cover 2, and a drainage interface 35 connected to the drainage gap 34 is provided on the base 3. After the drainage interface 35 is opened, the liquid filtered by the sieve plate 6 can be discharged from the drainage gap 34 and the drainage interface 35 in sequence, and the structure is simple and effective.

[0040] The method of using the utility model dynamic axial compression chromatographic column is as follows:

[0041] During the homogenate stage, one of the two homogenate inlets 31 is connected to the liquid, and the other is closed. The cylinder as the driving member 5 works to make the blocking member 4 move downward to the bottom of the liquid inlet channel 33, and the homogenate inlet 31, the liquid inlet channel 33 and the homogenate outlet 32 ​​are connected. At the same time, the second sealing ring 43 seals and blocks the bottom of the liquid inlet channel 33 to prevent leakage. The homogenate enters from the homogenate inlet 31 on one side and enters the column tube 1 through the homogenate outlet 32.

[0042] After the homogenate liquid is injected, the cylinder works to make the plugging member 4 move upward to the top, the sealing cover 41 is pressed to seal the homogenate outlet 32, and the first sealing ring 42 seals the gap between the upper end of the plugging member 4 and the upper end of the liquid inlet channel 33. After the drain port 35 is opened, the liquid filtered by the sieve plate 6 can be discharged from the drain gap 34 and the drain port 35 in sequence.

[0043] Although the present invention has been disclosed as a preferred embodiment, it is not intended to limit the present invention. Any technician familiar with the art can use the above disclosed technical content to make many possible changes and modifications to the technical solution of the present invention without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention should fall within the scope of protection of the technical solution of the present invention.

Claims

1. A dynamic axial compression chromatographic column, comprising a column tube (1), a lower cover (2) arranged at the lower end of the column tube (1), and a base (3) connected to the lower cover (2), characterized in that: The invention also comprises a blocking member (4) and a driving member (5) for driving the blocking member (4) to extend and retract. A homogenization inlet (31) is provided on the side wall of the base (3) and a homogenization outlet (32) is provided on the top. The homogenization inlet (31) and the homogenization outlet (32) are connected via a liquid inlet channel (33) provided inside the base (3). The upper end of the blocking member (4) is provided in the liquid inlet channel (33).

2. The dynamic axial compression chromatographic column according to claim 1, characterized in that: A sealing cover (41) is provided on the top of the blocking member (4).

3. The dynamic axial compression chromatographic column according to claim 2, characterized in that: A first sealing ring (42) is provided between the upper end of the sealing member (4) and the base (3), and a second sealing ring (43) is provided between the lower end of the sealing member (4) and the base (3).

4. The dynamic axial compression chromatographic column according to claim 3, characterized in that: The diameter of the upper end of the liquid inlet channel (33) is smaller than the diameter of the lower end.

5. The dynamic axial compression chromatographic column according to claim 1, characterized in that: A sieve plate (6) is provided above the lower cover (2), and the top of the base (3) is flush with the upper surface of the sieve plate (6).

6. The dynamic axial compression chromatographic column according to claim 1, characterized in that: The base (3) and the lower cover (2) are fixedly connected via fasteners.

7. The dynamic axial compression chromatography column according to any one of claims 1 to 6, characterized in that: The driving member (5) is a telescopic structure.

8. The dynamic axial compression chromatographic column according to claim 7, characterized in that: The driving member (5) is a cylinder, and the piston rod (51) of the cylinder is connected to the lower end of the blocking member (4).

9. The dynamic axial compression chromatographic column according to claim 8, characterized in that: The lower end of the piston rod (51) is provided with a scale mark and passes through the cylinder body (52) of the cylinder.

10. The dynamic axial compression chromatography column according to any one of claims 1 to 6, characterized in that: A liquid discharge gap (34) is provided between the outer wall of the base (3) and the lower cover (2), and a liquid discharge interface (35) communicating with the liquid discharge gap (34) is provided on the base (3).

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