Vacuum filtration and purification device for extracting organic selenium in food

Abstract

The utility model relates to the technical field of extraction of organic selenium in food, in particular to a vacuum filtration and purification device for extraction of organic selenium in food, and adopts the technical scheme that the vacuum filtration and purification device comprises an air pump body, a storage box and a purification bin, the air pump body is fixedly mounted on the front surface of the storage box, the purification bin comprises a bottom bin, and a top bin is movably mounted on the upper surface of the bottom bin; a hole is formed in the top of the inner side of the top bin and is communicated with a communicating pipe, a sampling pipe is movably inserted into the communicating pipe, a placing frame is movably installed in the bottom bin, a material receiving pipe is movably inserted into the placing frame and is located under the sampling pipe, and a hole is formed in the rear end of the bottom bin and is communicated with the sucking pump body through a guide pipe. According to the invention, modernization and high efficiency of an experimental sample preparation process are realized, a reliable and efficient operation platform is provided for the experimental process, meanwhile, the whole preparation process is more active and efficient, and the common problems of slow dripping, filtering blockage and the like in gravity filtration are avoided.

Description

Technical Field

[0001] The invention relates to the technical field of organic selenium extraction in food, in particular to a vacuum filtration and purification device for extracting organic selenium in food. Background Art

[0002] Selenium is an essential trace element with many important physiological functions. Although the amount of selenium in the human body is small, it is essential for maintaining normal physiological functions of the human body. Selenium is the core component of glutathione peroxidase (GPx), which is one of the main antioxidant enzymes that can scavenge free radicals and protect cells from oxidative damage. It is also involved in the formation of thioredoxin reductase and selenoprotein P. These enzymes and proteins have antioxidant functions. Selenium plays an important role in maintaining the normal function of the immune system and promotes the growth and activity of immune cells. Studies have shown that proper selenium intake can enhance the function of T cells and killer cells in the body and improve immune response capabilities. Selenium is an essential trace element in the metabolism of thyroid hormones. It participates in the conversion of T4 (thyroid hormone) into the active form of T3, which helps to maintain... Protect thyroid cells from oxidative damage caused by hydrogen peroxide and maintain thyroid health. Some studies have shown that moderate selenium intake is associated with a reduced risk of certain types of cancer (such as prostate cancer, lung cancer and colon cancer). Selenium inhibits the growth and metastasis of cancer cells through its antioxidant and immune-enhancing effects. The antioxidant effect of selenium helps prevent the oxidation of low-density lipoprotein (LDL) cholesterol, thereby reducing the risk of atherosclerosis. Selenium can also regulate inflammatory responses and reduce the incidence of cardiovascular disease. Appropriate selenium intake has a positive effect on brain function and mental health. Low selenium levels may be associated with mental health problems such as anxiety and depression.

[0003] The steps for organic selenium extraction are as follows:

[0004] 1. Sample Preparation

[0005] After freeze-drying, the sample is crushed or ground into powder under liquid nitrogen environment and passed through a 100-mesh sieve. After sample preparation, it is bagged with the date, sample information, fineness, etc. written on it and stored in a dryer for future use.

[0006] 2. Sample enzymatic digestion

[0007] Weigh 1 g (accurate to 0.0001 g) of the prepared sample into a 40 mL glass bottle, add enzymes: add 0.05 g of protease XIV and 0.05 g of cellulase, add 15 mL of deionized water (18.2 MQ·cm) and 10 mL of methanol, cover with a polytetrafluoroethylene lid, shake on an oscillator, perform ultrasonic enzymolysis in a 37°C water bath (30 min, power 80 W, frequency 40 kHz), shake at 200 / min after ultrasonication, and oscillate in a constant temperature oscillator at 37°C for 12 h.

[0008] 3. Sample extraction, concentration and impurity removal

[0009] Add 10 mL of dichloromethane to the fully enzymatically hydrolyzed sample, shake for 1 minute, and place in a centrifuge. Centrifuge at 1500 r / min for 10 minutes. Transfer all the upper methanol-water phase (25 mL) to a 50 mL conical polypropylene tube. Accurately pipette 5.0 mL of sample enzymatic supernatant from the conical polypropylene tube and transfer it to a flat-bottomed conical flask for total selenium testing. The remaining 20 mL of supernatant is placed in a vacuum refrigerated centrifugal concentrator with the cold trap temperature set to -105°C until completely dry. Take out the dried sample, reconstitute it with 2 mL of deionized water, and purify it with a Sep-PakC18 solid phase extraction column. Elute with 3 mL of methanol, and then remove excess methanol using a refrigerated centrifugal concentrator. Concentrate to less than 2 mL, transfer to an injection bottle, and dilute to 2 mL with deionized water before testing the selenium form.

[0010] In the above steps, especially when performing filtering and purification operations, excessive reliance is placed on gravity filtration, which results in a time-consuming problem. At the same time, during the operation, different devices are far apart, and operators need to run back and forth between different devices, resulting in a waste of time and the risk of sample spillage. Therefore, a vacuum filtration and purification device for extracting organic selenium from food is urgently needed to improve work efficiency. Summary of the invention

[0011] The purpose of the present invention is to provide a vacuum filtration and purification device for extracting organic selenium in food, which can effectively solve the problems in the background technology.

[0012] To achieve the above object, the present invention provides the following technical solution: a vacuum filtration purification device for extracting organic selenium from food, comprising a vacuum pump body, a storage box, a storage tank and a purification chamber,

[0013] The storage box comprises a storage bin, a cover body is movably mounted on the top of the storage bin, a refrigeration mechanism body is fixedly mounted on the back of the storage bin, a base frame is fixedly mounted on the bottom of the storage bin, and a placement plate is fixedly mounted on one side of the base frame;

[0014] The air pump body is fixedly mounted on the front of the storage box;

[0015] The placement tank is fixedly mounted on the rear end of the upper surface of the placement plate, a crushing bin is movably inserted in the placement tank, a material taking tank is threadedly mounted on the bottom end of the crushing bin, a knife holder is movably mounted in the crushing bin, and a filter screen is embedded and mounted on the inner bottom of the crushing bin;

[0016] The purification chamber is fixedly installed on the front end of the placement plate, and the purification chamber includes a bottom chamber, a top chamber is movably installed on the upper surface of the bottom chamber, the top inner side of the top chamber has a hole and is connected to a connecting pipe, a sampling tube is movably inserted in the connecting pipe, a placement rack is movably installed in the bottom chamber, a material receiving pipe is movably inserted in the placement rack, and the material receiving pipe is located directly below the sampling tube, and a hole is opened at the rear end of the bottom chamber and is connected to the vacuum pump body through a conduit.

[0017] Preferably, a reserved hole is opened at the bottom of the storage bin, a connecting groove is installed in communication with the bottom of the storage bin below the reserved hole, and an end of the connecting groove away from the storage bin is connected and installed with the refrigeration mechanism body.

[0018] Preferably, a connecting belt is fixedly installed on one side of the upper surface of the cover body, one end of the connecting belt facing away from the cover body is movably engaged with the storage bin, and the other side of the upper surface of the cover body is rotatably connected to the storage bin via a hinge.

[0019] Preferably, universal wheels are fixedly installed at the four corners of the bottom of the chassis. The setting of universal wheels improves the mobility of the equipment, improves experimental efficiency, protects the equipment and the ground, and provides a safe and stable operating environment. By selecting high-quality, wear-resistant and moderately load-bearing universal wheels, and equipping them with necessary locking devices, it can be ensured that the storage box has both flexible mobility and stability during use, thereby significantly optimizing the laboratory's operating procedures and safety.

[0020] Preferably, a sealing cover is threadedly installed on the top of the crushing bin, a driving motor is fixedly installed at the center of the upper surface of the sealing cover, the driving motor is transmission-mounted with the tool holder, and the driving motor drives the tool holder to perform a crushing operation on the sample in the crushing bin.

[0021] Preferably, the outer diameter of the material taking tank matches the outer diameter of the crushing bin, and the outer diameter of the material taking tank matches the inner diameter of the top opening of the placement tank.

[0022] Preferably, a sealing strip is fixedly installed on the lower end surface of the top bin, and the top bin is movably inserted on the top of the bottom bin through the sealing strip. The setting of the sealing strip improves the sealing performance of the opposite side of the top bin and the bottom bin, thereby avoiding air leakage after negative pressure is formed.

[0023] Preferably, a sealing ring is fixedly installed on the outer wall of the sampling tube, the outer diameter of the sealing ring is larger than the inner diameter of the connecting tube, and a solid phase extraction column is movably installed in the sampling tube. The setting of the sealing ring allows the lower part of the sealing ring to enter the connecting tube through the negative pressure environment after the sampling tube is inserted into the top bin, thereby improving the air tightness.

[0024] Preferably, a limit rod is fixedly installed at the center of the bottom circle of the placement rack, and the cross-section of the limit rod adopts a hexagonal structure design. A limit tube is fixedly installed at the center of the inner side of the bottom bin below the limit rod, and the limit rod is inserted in the limit tube. The hexagonal cross-section design can avoid the situation where the material receiving tube is difficult to align with the sampling tube after the placement rack is installed.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The present invention provides a purification chamber, and a vacuum pump body can be used to generate a negative pressure environment in the purification chamber, so that the sample solution in the purification chamber can quickly pass through the solid phase extraction column in the sampling tube under the action of the vacuum pump body and drip into the receiving tube, thereby improving the filtration speed and significantly reducing the filtration time. At the same time, the operator does not need to frequently monitor and intervene in the filtration process. Compared with gravity filtration, the degree of automation is higher, and the workload of the operator is reduced. The purification chamber creates a negative pressure environment on the side opposite to the bottom chamber and the top chamber through the vacuum pump body, so that the liquid is subjected to additional driving force when passing through the filter medium solid phase extraction column, thereby accelerating the speed of the liquid passing through the filter medium. Compared with slow filtration relying on gravity, negative pressure filtration can significantly reduce the time required for filtering the liquid, and the sample to be processed can flow out of the filter column faster. Under the action of negative pressure, the liquid is actively filtered instead of relying on gravity to fall, which makes the whole process more active and efficient, avoiding the common problems of slow dripping and filter blockage in gravity filtration.

[0027] The present invention integrates the storage bin, refrigeration mechanism, vacuum pump body, storage tank and purification bin into one system, thereby reducing the need for experimenters to run back and forth between different devices and switch operations, simplifying the experimental process and improving operating efficiency. The storage box provides a low-temperature and stable environment to ensure the effective storage of experimental samples and prevent sample deterioration or contamination. At the same time, liquid nitrogen can be injected into the storage tank for low-temperature crushing, thereby low-temperature crushing is carried out through the crushing bin, thereby ensuring that the sample is quickly crushed at a low temperature and reducing thermal denaturation of the sample and loss of active ingredients. The filter screen at the bottom of the crushing bin helps to quickly screen the sample, thereby improving the efficiency and accuracy of sample preparation, and integrating freezing, crushing, screening, filtration, etc. The functions are integrated in one device to form an integrated operating system, which simplifies the operating steps and reduces the complexity of operation. The operator only needs to perform all experimental operations on the same device, which reduces the time for arranging and transferring samples, and makes the operation simpler and more intuitive. The integration and reasonable layout of the overall device significantly improve the efficiency of the experimental process, reduce the time waste caused by equipment dispersion and operation separation, and make the entire experimental process more coherent and efficient. Through low-temperature crushing and rapid filtration, the original composition and activity of the sample are retained to the maximum extent, and the preparation quality of the experimental sample is improved. Through the optimization and integration of the above equipment, the modernization and efficiency of the experimental sample preparation process are realized, providing a reliable and efficient operating platform for the experimental process. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the main structure of the present invention;

[0029] Figure 2 It is a schematic diagram of the top view structure of the present invention;

[0030] Figure 3 It is a schematic diagram of the cross-sectional structure of the purification chamber of the present invention;

[0031] Figure 4 For the present invention Figure 3 The structural diagram of the enlarged part in the middle;

[0032] Figure 5 It is a schematic diagram of the cross-sectional structure of the present invention;

[0033] Figure 6 It is a schematic diagram of the main structure of the refrigeration mechanism of the present invention;

[0034] Figure 7 It is a schematic diagram of the cross-sectional structure of the placement tank of the present invention;

[0035] Figure 8 For the present invention Figure 7 Schematic diagram of the structure at the enlarged point.

[0036] In the figure: 1. vacuum pump body; 2. storage box; 3. storage tank; 4. purification chamber; 5. refrigeration mechanism body; 6. bottom chamber; 7. sealing strip; 8. top chamber; 9. sampling tube; 10. storage rack; 11. limiting rod; 12. limiting tube; 13. material receiving tube; 14. connecting tube; 15. solid phase extraction column; 16. sealing ring; 17. connecting belt; 18. cover body; 19. hinge; 20. storage plate; 21. universal wheel; 22. bottom frame; 23. connecting groove; 24. reserved hole; 25. storage chamber; 26. sealing cover; 27. driving motor; 28. knife holder; 29. ​​material tank; 30. crushing chamber; 31. filter screen. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0038] like Figures 1 to 6 As shown, an embodiment of the present invention is a vacuum filtration purification device for extracting organic selenium in food, comprising a vacuum pump body 1, a storage box 2 and a purification chamber 4.

[0039] The storage box 2 includes a storage bin 25, a cover 18 is movably mounted on the top of the storage bin 25, a connecting belt 17 is fixedly mounted on one side of the upper surface of the cover 18, one end of the connecting belt 17 away from the cover 18 is movably engaged with the storage bin 25, and the other side of the upper surface of the cover 18 is rotatably connected to the storage bin 25 through a hinge 19. One end of the connecting belt 17 is fixedly mounted on the upper surface of the cover 18, and the other end is movably engaged with the storage bin 25, so as to fix the cover 18 and prevent it from accidentally falling off or shifting. The cover 18 can be smoothly rotated by the hinge 19, which is convenient for opening and closing operations.

[0040] The refrigeration mechanism body 5 is fixedly installed on the back of the storage bin 25, and a reserved hole 24 is opened at the bottom of the storage bin 25. A connecting groove 23 is connected and installed at the bottom of the storage bin 25 below the reserved hole 24. The end of the connecting groove 23 away from the storage bin 25 is connected and installed with the refrigeration mechanism body 5. A bottom frame 22 is fixedly installed at the bottom of the storage bin 25, and universal wheels 21 are fixedly installed at the four corners of the bottom of the bottom frame 22. A placement plate 20 is fixedly installed on one side of the bottom frame 22.

[0041] The vacuum pump body 1 is fixedly mounted on the front of the storage box 2 and is used with a rotary vane vacuum pump or a diaphragm pump. Both types can provide a stable negative pressure environment and are suitable for filtering and purifying laboratory samples.

[0042] The purification chamber 4 is fixedly mounted on the front end of the upper end face of the placement plate 20. The purification chamber 4 includes a bottom chamber 6. A top chamber 8 is movably mounted on the upper surface of the bottom chamber 6. A sealing strip 7 is fixedly mounted on the lower end face of the top chamber 8. The top chamber 8 is movably inserted on the top of the bottom chamber 6 through the sealing strip 7. A connecting pipe 14 is installed in the top inner side of the top chamber 8 and connected thereto. A sampling tube 9 is movably inserted in the connecting tube 14. A sealing ring 16 is fixedly mounted on the upper outer wall of the sampling tube 9. The outer diameter of the sealing ring 16 is larger than the inner diameter of the connecting tube 14. A solid phase extraction column 15 is movably mounted in the sampling tube 9.

[0043] A placing rack 10 is movably installed in the bottom bin 6, a limiting rod 11 is fixedly installed at the center of the bottom of the placing rack 10, and the cross section of the limiting rod 11 adopts a hexagonal structure design, and a limiting tube 12 is fixedly installed at the center of the inner side of the bottom bin 6 below the limiting rod 11, and the limiting rod 11 is inserted in the limiting tube 12. A material receiving pipe 13 is movably inserted in the placing rack 10, and the material receiving pipe 13 is located directly below the sampling tube 9. The rear end of the bottom bin 6 is opened and connected to the vacuum pump body 1 through a conduit. Example

[0044] like Figure 7 and Figure 8 As shown, an embodiment of the present invention is a vacuum filtration purification device for extracting organic selenium in food, comprising a vacuum pump body 1, a storage box 2, a storage tank 3 and a purification chamber 4.

[0045] The storage box 2 includes a storage compartment 25 , a cover 18 is movably mounted on the top of the storage compartment 25 , a base frame 22 is fixedly mounted on the bottom of the storage compartment 25 , and a placement plate 20 is fixedly mounted on one side of the base frame 22 .

[0046] The placement tank 3 is fixedly installed at the rear end of the upper end surface of the placement plate 20, and a crushing bin 30 is movably inserted in the placement tank 3. A sealing cover 26 is threadedly installed on the top of the crushing bin 30. A driving motor 27 is fixedly installed at the center of the upper surface of the sealing cover 26, and the driving motor 27 is transmission-installed with the tool holder 28.

[0047] The bottom end of the crushing bin 30 is threadedly installed with a material taking tank 29, and the outer diameter of the material taking tank 29 matches the outer diameter of the crushing bin 30, and the outer diameter of the material taking tank 29 matches the inner diameter of the top opening of the placement tank 3. A knife holder 28 is movably installed in the crushing bin 30, and a filter screen 31 is embedded and installed at the bottom of the inner side of the crushing bin 30. The filter screen 31 is 100 mesh. Compared with the coarser filter screen 30, the 100 mesh filter screen 31 provides higher precision screening to ensure the consistency and quality of the sample.

[0048] When the present invention is used, it is ensured that the refrigeration mechanism body 5 is connected and started, and can provide a low-temperature environment, the cover 18 of the storage bin 25 is opened, and the sample is placed in the storage bin 25 for temporary storage, and the cover 18 is closed to ensure that the sample is effectively preserved at a low temperature; the placement tank 3 is opened, the crushing bin 30 is taken out, the sample is taken out and injected into the crushing bin 30, and the top of the crushing bin 30 is closed by the sealing cover 26, so that the knife holder 28 extends into the crushing bin 30; liquid nitrogen is injected into the placement tank 3, and the material collection tank 29 is installed at the bottom of the crushing bin 30, and the crushing bin 30 and the material collection tank 29 are placed in the placement tank 3 together, so as to ensure that the sample is crushed in a low-temperature environment, and the start The drive motor 27 is driven to perform low-temperature crushing through the tool holder 28; after the crushing is completed, the liquid nitrogen evaporates and the drive motor is turned off; at this time, the crushed sample is preliminarily screened through the filter screen 31 at the bottom of the crushing bin 30 and falls into the material tank 29; the top bin 8 of the purification bin 4 is opened, and the placement rack 10 is placed in the bottom bin 6, and after closing the top bin 8, it is ensured that the material receiving pipe 13 is located directly below the sampling tube 9; the sample solution in the material tank 29 is poured into the sampling tube 9; the vacuum pump body 1 is started, and through the negative pressure environment of the bottom bin 6 and the top bin 8, the sample solution quickly passes through the solid phase extraction column 15 and finally drips into the material receiving pipe 13; the vacuum pump is turned off to take out the purified sample in the material receiving pipe 13.

Claims

1. A vacuum filtration purification device for extracting organic selenium from food, comprising a vacuum pump body (1), a storage box (2), a storage tank (3) and a purification chamber (4), characterized in that: The storage box (2) comprises a storage bin (25), a cover body (18) is movably mounted on the top of the storage bin (25), a refrigeration mechanism body (5) is fixedly mounted on the back of the storage bin (25), a base frame (22) is fixedly mounted on the bottom of the storage bin (25), and a placement plate (20) is fixedly mounted on one side of the base frame (22); The air pump body (1) is fixedly mounted on the front of the storage box (2); The placement tank (3) is fixedly mounted on the rear end of the upper end surface of the placement plate (20); a crushing bin (30) is movably inserted in the placement tank (3); a material taking tank (29) is threadedly mounted on the bottom end of the crushing bin (30); a knife holder (28) is movably mounted in the crushing bin (30); and a filter screen (31) is embedded and mounted on the inner bottom of the crushing bin (30); The purification chamber (4) is fixedly mounted on the front end of the upper end of the placement plate (20), and the purification chamber (4) comprises a bottom chamber (6), a top chamber (8) is movably mounted on the upper surface of the bottom chamber (6), a hole is opened at the top of the inner side of the top chamber (8) and a connecting pipe (14) is installed in communication therewith, a sampling tube (9) is movably inserted in the connecting pipe (14), a placement rack (10) is movably installed in the bottom chamber (6), a material receiving pipe (13) is movably inserted in the placement rack (10), and the material receiving pipe (13) is located directly below the sampling tube (9), and a hole is opened at the rear end of the bottom chamber (6) and is connected and mounted to the vacuum pump body (1) through a conduit.

2. The vacuum filtration purification device for extracting organic selenium from food according to claim 1, characterized in that: A reserved hole (24) is provided at the bottom of the storage bin (25), a connecting groove (23) is installed in communication with the bottom of the storage bin (25) below the reserved hole (24), and an end of the connecting groove (23) facing away from the storage bin (25) is installed in communication with the refrigeration mechanism body (5).

3. The vacuum filtration purification device for extracting organic selenium from food according to claim 1, characterized in that: A connecting belt (17) is fixedly mounted on one side of the upper surface of the cover body (18); one end of the connecting belt (17) facing away from the cover body (18) is movably engaged with the storage bin (25); and the other side of the upper surface of the cover body (18) is rotatably connected to the storage bin (25) via a hinge (19).

4. The vacuum filtration and purification device for extracting organic selenium from food according to claim 1, characterized in that: Universal wheels (21) are fixedly mounted at the four corners of the bottom of the base frame (22).

5. The vacuum filtration and purification device for extracting organic selenium from food according to claim 1, characterized in that: A sealing cover (26) is threadedly mounted on the top of the crushing bin (30), a driving motor (27) is fixedly mounted at the center of the upper surface of the sealing cover (26), and the driving motor (27) is transmission-mounted with the knife holder (28).

6. The vacuum filtration purification device for extracting organic selenium from food according to claim 1, characterized in that: The outer diameter of the material taking tank (29) matches the outer diameter of the crushing bin (30), and the outer diameter of the material taking tank (29) matches the inner diameter of the top opening of the placement tank (3).

7. The vacuum filtration purification device for extracting organic selenium from food according to claim 1, characterized in that: A sealing strip (7) is fixedly mounted on the lower end surface of the top bin (8), and the top bin (8) is movably inserted into the top of the bottom bin (6) via the sealing strip (7).

8. The vacuum filtration and purification device for extracting organic selenium from food according to claim 1, characterized in that: A sealing ring (16) is fixedly installed on the outer wall of the sampling tube (9); the outer diameter of the sealing ring (16) is larger than the inner diameter of the connecting tube (14); and a solid phase extraction column (15) is movably installed in the sampling tube (9).

9. The vacuum filtration purification device for extracting organic selenium from food according to claim 1, characterized in that: A limit rod (11) is fixedly installed at the center of the bottom circle of the placement rack (10), and the cross section of the limit rod (11) adopts a hexagonal structure design. A limit tube (12) is fixedly installed at the center of the inner circle of the bottom bin (6) below the limit rod (11), and the limit rod (11) is inserted into the limit tube (12).

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