Sequencing equipment and sequencing system

Abstract

The utility model discloses sequencing equipment and a sequencing system. The sequencing equipment comprises a base with an opening, wherein the opening is formed in the surface, perpendicular to a first direction, of the base; the sample loading part is at least partially positioned in the opening and is used for loading a sequencing chip; the top cover assembly comprises a top cover body, the top cover body is movably connected with the base, and the top cover body is used for covering or exposing the opening; the liquid injection assembly is at least partially located in the base, the liquid injection assembly comprises a rotary valve and a pipeline system, and the rotary valve is arranged in the pipeline system; the driving assembly comprises a driving control part, a top cover driving part and a liquid injection driving part, the driving control part is arranged in the base and used for controlling the top cover driving part and / or the liquid injection driving part to operate, and the top cover driving part is used for driving the top cover body to move relative to the base so as to cover or expose the opening; the liquid injection driving part is used for driving the liquid injection assembly to inject liquid. According to the sequencing equipment and the sequencing system disclosed by the invention, the automation degree can be improved, and the influence of human factors on the sequencing result is reduced.

Description

Technical Field

[0001] The present application relates to the field of gene sequencing technology, and in particular to a sequencing device and a sequencing system. Background Art

[0002] Gene sequencer, also known as DNA sequencer, is an instrument that determines the base sequence, type and quantity of DNA fragments. It is mainly used in human genome sequencing, genetic diagnosis of human genetic diseases, infectious diseases and cancer, forensic paternity testing and individual identification, bioengineering drug screening, animal and plant hybrid breeding, etc.

[0003] When using a gene sequencer, the user is usually required to place the test object in the gene sequencer and manually close the gene sequencer. Due to the introduction of manual operation, there is a risk that external unstable factors will affect the sequencing results and sequencing accuracy. Summary of the invention

[0004] The embodiments of the present application provide a sequencing device and a sequencing system, which can improve the degree of automation and reduce the impact of human factors on sequencing results.

[0005] In a first aspect, an embodiment of the present application provides a sequencing device, comprising: a base, having an opening, the opening being located on a surface of the base perpendicular to a first direction; a sample loading portion, at least partially located in the opening, for carrying a sequencing chip; a top cover assembly, comprising a top cover body, the top cover body being movably connected to the base, the top cover body being used to cover or expose the opening; an injection assembly, at least partially located in the base, the injection assembly comprising a rotary valve and a pipeline system, the rotary valve being arranged in the pipeline system; a drive assembly, comprising a drive control portion, a top cover drive portion and an injection drive portion, the drive control portion being arranged in the base and for controlling the operation of the top cover drive portion and / or the injection drive portion, the top cover drive portion being used to drive the top cover body to move relative to the base, thereby covering or exposing the opening; and the injection drive portion being used to drive the injection assembly to inject liquid.

[0006] According to an embodiment of the first aspect of the present application, the injection component also includes an injection pump, which is connected to the pipeline system, and the injection drive unit is used to drive the injection pump to inject liquid into the sequencing chip.

[0007] According to an embodiment of the first aspect of the present application, the liquid injection drive unit is used to control the rotation of the rotary valve, thereby opening or closing the pipeline system.

[0008] According to an embodiment of the first aspect of the present application, the top cover assembly also includes a first rotating shaft and a second rotating shaft, which are respectively connected to the two ends of the top cover body along the second direction; the second rotating shaft is rotatably connected to the top cover driving part; the base is provided with a rolling groove extending along the second direction, and the first rotating shaft is movably arranged in the rolling groove.

[0009] According to an embodiment of the first aspect of the present application, the top cover driving unit includes a driving motor, a transmission wheel group, a transmission shaft, and a rocker arm. The driving motor is arranged in the base, the driving motor is power-connected to the transmission wheel group, the transmission wheel group is power-connected to the transmission shaft, the transmission shaft is rotatably connected to the base, one end of the rocker arm is fixedly connected to the transmission shaft, and the other end of the rocker arm is rotatably connected to the second rotating shaft.

[0010] According to an embodiment of the first aspect of the present application, the top cover driving unit also includes a photoelectric switch, and the photoelectric switch is arranged on the transmission shaft.

[0011] According to an embodiment of the first aspect of the present application, the sequencing device further includes a temperature control unit, which is located in the opening and is used to raise and lower the temperature of the sequencing chip.

[0012] According to an embodiment of the first aspect of the present application, the sequencing device further includes an operating unit, which is disposed on the base and connected to the sample loading unit.

[0013] According to an embodiment of the first aspect of the present application, the sequencing device further includes a main control component, which is disposed in the base and is used to control the sequencing operation of the sequencing chip.

[0014] In a second aspect, an embodiment of the present application provides a sequencing system, including: a sequencing device according to an embodiment of the first aspect of the present application; a host, electrically connected to a drive control unit, and configured to send operating instructions to the drive control unit.

[0015] In the sequencing device and sequencing system provided in the embodiments of the present application, the sample loading part partially exposed to the opening can carry the sequencing chip, the top cover body of the top cover assembly can be movably connected to the base, and the top cover driving part can drive the top cover body to move relative to the base, so that the opening of the base is exposed, or the top cover body covers the opening, thereby realizing the automatic opening and closing of the sequencing device in the embodiments of the present application; the injection driving part drives the injection assembly to inject liquid, and draws the sequencing sample into the sequencing chip for sequencing, thereby realizing the automatic start and stop of injection of the sequencing device in the embodiments of the present application; the drive control part can control the top cover driving part and the injection driving part, so that through the drive control part, the sequencing device in the embodiments of the application can improve the degree of automation of the sequencing process, reduce the influence of human factors, and improve the accuracy and reliability of sequencing. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1A schematic diagram of the structure of the sequencing device in the closed state according to an embodiment of the present application.

[0018] Figure 2 A schematic diagram of the structure of the sequencing device in an open state according to an embodiment of the present application.

[0019] Figure 3 A schematic diagram of the structure of the base of the sequencing device of an embodiment of the present application with the rolling groove hidden.

[0020] Figure 4 A schematic diagram of the principle of the liquid injection component of the sequencing device according to an embodiment of the present application.

[0021] Figure 5 A signal conduction schematic diagram of a driving component of a sequencing device according to an embodiment of the present application.

[0022] Figure 6 A top view of the sequencing device according to an embodiment of the present application in an open state.

[0023] Figure 7 for Figure 6 A local enlarged view of region A when the rotary valve of the sequencing device of an embodiment of the present application is in the second state.

[0024] Figure 8 for Figure 6 A partial enlarged view of region A when the rotary valve of the sequencing device of an embodiment of the present application is in the first state.

[0025] Fig. 9 A schematic diagram of the structure of the liquid injection drive unit of the sequencing device according to an embodiment of the present application.

[0026] Fig.10 A schematic diagram of the structure of the top cover assembly, the top cover driving unit and the rolling groove of the sequencing device according to an embodiment of the present application.

[0027] Fig.11 A schematic diagram of the top cover assembly, top cover driving unit and rolling groove of the sequencing device according to an embodiment of the present application.

[0028] Fig.12 Another schematic diagram of the top cover assembly, top cover driving unit and rolling groove of the sequencing device according to an embodiment of the present application.

[0029] Fig.13 for Figure 2 A local enlarged view of region B of the sequencing device of an embodiment of the present application.

[0030] Fig.14 A schematic diagram of the structure of the temperature control unit and the support unit of the sequencing device according to an embodiment of the present application.

[0031] Reference numerals:

[0032] 1. Base; 11. Opening; 12. Rolling groove;

[0033] 2. Top cover assembly; 21. Top cover body; 22. First rotating shaft; 23. Second rotating shaft;

[0034] 3. Liquid injection assembly; 31. Liquid injection pump; 32. Pipeline system; 33. Rotary valve;

[0035] 4. driving assembly; 41. driving control unit; 42. top cover driving unit; 421. driving motor; 422. transmission wheel set; 423. transmission shaft; 424. swing rod; 425. photoelectric switch; 43. liquid injection driving unit; 431. rotating unit;

[0036] 5. Temperature control department;

[0037] 6. Operation department;

[0038] 8. Sample loading unit;

[0039] 100, host; 200, sequencing equipment;

[0040] X, first direction; Y, second direction. DETAILED DESCRIPTION

[0041] The features and exemplary embodiments of various aspects of the application will be described in detail below. In the detailed description below, many specific details are proposed to provide a comprehensive understanding of the application. However, it is obvious to those skilled in the art that the application can be implemented when some details in these specific details are not needed. The following description of the embodiments is only to provide a better understanding of the application by illustrating the example of the application.

[0042] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The embodiments will be described in detail below in conjunction with the accompanying drawings.

[0043] Relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article, or device. In the absence of further restrictions, the elements defined by the statement "comprising ..." do not exclude the presence of additional identical elements in the process, method, article, or device that includes the elements.

[0044] It should be understood that when describing the structure of a component, when a layer or a region is referred to as being "on" or "over" another layer or another region, it may mean that it is directly on the other layer or another region, or that other layers or regions are included between it and the other layer or another region. Moreover, if the component is turned over, the layer or a region will be "below" or "beneath" another layer or another region.

[0045] In addition, the term "and / or" in this article is only a description of the association relationship between the associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0046] It should be understood that in the embodiment of the present application, "B corresponding to A" means that B is associated with A, and B can be determined according to A. However, it should also be understood that determining B according to A does not mean determining B only according to A, and B can also be determined according to A and / or other information.

[0047] The applicant has found that in the process of gene sequencing, the operator is usually required to manually add the sequencing sample to the sequencing chip of the sequencer, and then manually turn off the sequencer, seal the sample and sequencing chip in the sequencer, and perform gene sequencing. During the sequencing process, since the operator needs to manually operate, there is the influence of external human factors, there is a risk of contaminating the sample or sequencing chip, and reducing the accuracy and reliability of gene sequencing.

[0048] In view of the above analysis, the applicant has proposed a sequencing device and a sequencing system. The sequencing device includes a base, a sample loading part, a top cover assembly, a liquid injection assembly and a drive assembly. The sample loading part partially exposed to the opening can carry the sequencing chip, the top cover body of the top cover assembly can be movably connected to the base, and the top cover drive can drive the top cover body to move relative to the base, so that the opening of the base is exposed, or the top cover body covers the opening, so as to realize the automatic opening and closing of the sequencing device of the embodiment of the present application; the liquid injection drive drives the liquid injection assembly to inject liquid, generates negative pressure in the pipeline system and the chip, and sucks the sequencing sample into the sequencing chip, so as to realize the automatic opening and stopping of liquid injection of the sequencing device of the embodiment of the present application; the drive control part can control the top cover drive part and the liquid injection drive part, so through the drive control part, the sequencing device of the embodiment of the application can improve the degree of automation of the sequencing process, reduce the influence of human factors, and improve the accuracy and reliability of sequencing.

[0049] Figure 1 A schematic diagram of the structure of the sequencing device in the closed state according to an embodiment of the present application. Figure 2A schematic diagram of the structure of the sequencing device in an open state according to an embodiment of the present application. Figure 3 A schematic diagram of the structure of the base of the sequencing device of an embodiment of the present application with the rolling groove hidden. Figure 4 A schematic diagram of the principle of the liquid injection component of the sequencing device according to an embodiment of the present application. Figure 5 A signal conduction schematic diagram of a driving component of a sequencing device according to an embodiment of the present application.

[0050] See also Figures 1 to 5 The embodiment of the present application proposes a sequencing device 200, comprising: a base 1, having an opening 11, wherein the opening 11 is located on a surface of the base 1 perpendicular to a first direction X; a sample loading portion 8, at least partially located in the opening, for carrying a sequencing chip; a top cover assembly 2, comprising a top cover body 21, wherein the top cover body 21 is movably connected to the base 1, and the top cover body 21 is used to cover or expose the opening 11; an injection assembly 3, at least partially located in the base 1, wherein the injection assembly 3 comprises a rotary valve 33 and a pipeline system 32, wherein the rotary valve 33 is arranged in the pipeline system; a drive assembly 4, comprising a drive control portion 41, a top cover drive portion 42, and an injection drive portion 43, wherein the drive control portion 41 is arranged in the base and is used to control the operation of the top cover drive portion 42 and the injection drive portion 43, wherein the top cover drive portion 42 is used to drive the top cover body 21 to move relative to the base 1, thereby covering or exposing the opening 11; and the injection drive portion 43 is used to drive the injection assembly 3 to inject liquid.

[0051] In the embodiment of the present application, the first direction X and the second direction Y intersect, and may be at an acute angle or perpendicular to each other. When the sequencing device 200 of the embodiment of the present application is placed on a horizontal plane, the second direction Y may be a horizontal direction, and the first direction X may be a vertical direction or a direction at an acute angle to the vertical direction. For ease of explanation, the embodiment of the present application is described by taking the first direction X as a vertical direction and the second direction Y as a horizontal direction as an example.

[0052] The base 1 serves as the main mounting carrier for other components of the sequencing device 200 of the embodiment of the present application. The base 1 has a surface perpendicular to the first direction X. Considering that the first direction X can be a vertical direction, the surface can be regarded as the top surface of the base 1. An opening 11 is provided on the top surface of the base 1, and the sequencing chip can be at least partially exposed at the opening 11, so as to facilitate the addition of the sequencing sample on the sequencing chip.

[0053] The sample loading part 8 is used to carry the sequencing chip. A part of the sample loading part 8 is located at the opening 11. The sequencing chip can be placed in the sample loading part 8. The sequencing chip and the sample loading part 8 can be electrically connected so that the sequencing chip can transmit signals with the sequencing device of the embodiment of the present application.

[0054] The top cover body 21 of the top cover assembly 2 is movably connected to the base 1. Through the movement of the top cover body 21 relative to the base 1, the top cover body 21 can cover the opening 11 of the base 1, thereby enclosing the sequencing chip inside the sequencing device 200 of the embodiment of the present application, reducing the impact of external factors on the sequencing results. In the embodiment of the present application, the movement of the top cover body 21 relative to the base 1 can include translation, rotation, or a combination of the two. When the top cover body 21 is buckled with the base 1, the interior of the sequencing device 200 of the embodiment of the present application can be in a sealed state or an unsealed state, but the possibility of external impurities entering the interior should be reduced.

[0055] At least a portion of the injection assembly 3 is disposed inside the base 1, and is used to add sequencing samples into the sequencing chip. The rotary valve 33 is disposed in the pipeline system 32, and can be used to control the opening or closing of the pipeline system 32. The rotary valve 33 is exposed at the opening 11. When there is a problem with the automatic control of the rotary valve 33 or other situations requiring manual control occur, the opening or closing of the rotary valve 33 can also be controlled manually.

[0056] The driving assembly 4 is used to realize the automatic control of the sequencing device 200 of the embodiment of the present application. The top cover driving unit 42 can drive the top cover body 21 to move relative to the base 1, so as to realize the automatic opening and closing of the sequencing device 200 of the embodiment of the present application. The injection driving unit 43 can be used to drive the injection assembly 3 to operate or stop the injection, so as to realize the automatic opening and stopping of the injection of the sequencing device 200 of the embodiment of the present application. The driving control unit 41 can be used to control the operation of the top cover driving unit 42 and / or the injection driving unit 43. The sequencing device 200 of the embodiment of the present application can realize the automatic control of the sequencing process through the driving control unit 41, so as to reduce the influence of human factors on the sequencing results and improve the accuracy and reliability of sequencing.

[0057] When the sequencing device 200 of the embodiment of the present application performs gene sequencing, after the sequencing chip is placed in the sequencing device 200 of the embodiment of the present application, the driving control unit 41 drives the rotary valve 33 to open through the liquid injection driving unit 43 to connect the pipeline system 32, and at the same time, the liquid injection component 3 generates negative pressure to inject the sequencing sample into the sequencing chip; the driving control unit 41 drives the rotary valve 33 to close through the liquid injection driving unit 43 to close the pipeline system 32; at the same time, the driving control unit 41 drives the top cover body 21 to move relative to the base 1 through the top cover driving unit 42, so that the top cover body 21 is buckled with the base 1, and the sequencing chip and the sequencing sample are sealed inside the sequencing device 200 of the embodiment of the present application.

[0058] After the sequencing is completed, the driving control unit 41 drives the top cover body 21 to move relative to the base 1 through the top cover driving unit 42, so that the opening 11 is in an exposed state. At this time, the sequencing chip is also in an exposed state, so as to facilitate the removal of the sequencing chip.

[0059] Furthermore, the injection component 3 also includes an injection pump 31, which is connected to the pipeline system 32. The injection pump 31 can be used to generate negative pressure, thereby generating negative pressure in the pipeline system 32 and the chip flow channel to inject the sequencing sample into the sequencing chip.

[0060] The injection pump 31 can be integrated in the base 1 or arranged outside the base 1. The driving component 4 sends an injection signal, and the injection pump 31 receives the injection signal, and the injection pump 31 starts to operate, so that the pipeline system 32 generates negative pressure, and the sequencing sample is sucked into the sequencing chip due to the negative pressure.

[0061] Figure 6 A top view of the sequencing device according to an embodiment of the present application in an open state. Figure 7 for Figure 6 A local enlarged view of region A when the rotary valve of the sequencing device of an embodiment of the present application is in the second state. Figure 8 for Figure 6 A partial enlarged view of region A when the rotary valve of the sequencing device of an embodiment of the present application is in the first state. Fig. 9 A schematic diagram of the structure of the liquid injection drive unit of the sequencing device according to an embodiment of the present application.

[0062] For further information, see Figures 6 to 9 The liquid injection driving unit 43 is used to control the rotation of the rotary valve 33 to open or close the pipeline system 32.

[0063] The injection driving part 43 includes a rotating part 431, which is connected to the rotary valve 33, and at least a part of the rotating part 431 is located at the opening 11; the rotating part 431 can drive the rotary valve 33 to rotate, and finally the rotary valve 33 is in the first state or the second state. When the rotary valve 33 is in the first state, the pipeline system 32 is opened; when the rotary valve 33 is in the second state, the pipeline system 32 is closed.

[0064] The rotating part 431 is connected to the rotary valve 33, and is used to drive the rotary valve 33 to rotate. Exemplarily, the liquid injection driving part 43 of the embodiment of the present application can use a motor to drive the rotating part 431 to rotate, thereby driving the rotary valve 33 to rotate, so that the rotary valve 33 is in the first state or the second state. The rotary valve 33 can switch between the first state and the second state. Considering that a part of the rotary valve is exposed to the opening 11, so as to facilitate manual control of the rotary valve, the part of the rotary valve exposed to the opening 11 can be provided with a protrusion along the first direction X, so as to facilitate manual control of the rotary valve.

[0065] Fig.10 A schematic diagram of the structure of the top cover assembly, the top cover driving unit and the rolling groove of the sequencing device according to an embodiment of the present application. Fig.11A schematic diagram of the top cover assembly, top cover driving unit and rolling groove of the sequencing device according to an embodiment of the present application. Fig.12 Another schematic diagram of the top cover assembly, top cover driving unit and rolling groove of the sequencing device according to an embodiment of the present application.

[0066] For further information, see Figures 10 to 12 The top cover assembly 2 also includes a first rotating shaft 22 and a second rotating shaft 23, which are respectively connected to the two ends of the top cover body 21 along the second direction Y; the second rotating shaft 23 is rotatably connected to the top cover driving part 42; the base 1 is provided with a rolling groove 12 extending along the second direction Y, and the first rotating shaft 22 is movably arranged in the rolling groove 12.

[0067] The first rotating shaft 22 and the second rotating shaft 23 of the top cover assembly 2 are respectively connected to the two ends of the top cover body 21 along the second direction Y. Among them, the end of the first rotating shaft 22 is inserted into the rolling groove 12 with the base 1, so that the first rotating shaft 22 can move along the rolling groove 12. The top cover driving part 42 is rotatably connected with the second rotating shaft 23, so that the top cover driving part 42, the second rotating shaft 23, the first rotating shaft 22 of the top cover body 21 and the rolling groove 12 form a crank slider structure. When the top cover driving part 42 drives the second rotating shaft 23 to move in an arc, the top cover body 21 can realize relative movement to the base 1, and this movement belongs to a composite movement of translation and rotation.

[0068] It is understandable that the number of the first rotating shaft 22 and the second rotating shaft 23 can both be one, passing through the top cover body 21, or at least one of the first rotating shaft 22 and the second rotating shaft 23 is in pairs and coaxially arranged. Exemplarily, in the embodiment of the present application, two first rotating shafts 22 are provided, and the two first rotating shafts 22 are coaxially arranged, and the two first rotating shafts 22 are each movably connected to a rolling groove 12; one second rotating shaft 23 is provided, passing through the top cover body 21.

[0069] Further, see Figures 10 to 12 The top cover driving unit 42 includes a driving motor 421, a transmission wheel group 422, a transmission shaft 423, and a rocker arm 424. The driving motor 421 is arranged in the base 1. The driving motor 421 is power-connected to the transmission wheel group 422, the transmission wheel group 422 is power-connected to the transmission shaft 423, the transmission shaft 423 is rotatably connected to the base 1, one end of the rocker arm 424 is fixedly connected to the transmission shaft 423, and the other end of the rocker arm 424 is rotatably connected to the second rotating shaft 23.

[0070] The top cover driving part 42 drives the top cover body 21 to move in the form of a motor. The driving motor 421 serves as the power source of the top cover driving part 42. The output end of the driving motor 421 drives the transmission shaft 423 to rotate through the transmission wheel group 422. The transmission wheel group 422 can be in the form of a gear group so that the transmission shaft 423 has an appropriate speed and torque through an appropriate transmission ratio. When the transmission shaft 423 rotates, the swing rod 424 connected to the transmission shaft 423 will rotate together. The swing rod 424 is rotationally connected to the second rotating shaft 23. Therefore, the swing rod 424, the second rotating shaft 23, the first rotating shaft 22 of the top cover body 21 and the rolling groove 12 form a crank slider structure, so that the driving motor 421 can drive the top cover body 21 to move relative to the base 1. Exemplarily, two swing rods 424 can be provided, and the two swing rods 424 are respectively connected to the two ends of the rotation 423, and the two swing rods 424 are respectively connected to the two ends of the second rotating shaft 23.

[0071] Further, continue to participate Figures 10 to 12 The top cover driving unit 42 further includes a photoelectric switch 425, which is disposed on the transmission shaft 423. Considering that the range of motion of the top cover body 21 relative to the bottom cover is limited, the swing rod 424 should not swing 360° without causing motion interference. The photoelectric switch 425 can detect the rotation angle of the transmission shaft, obtain the position of the swing rod 424, limit the extreme position of the swing of the swing rod 424, and further reflect the position of the top cover body 21 relative to the base 1, so as to better control the movement of the top cover body 21.

[0072] Fig.13 for Figure 2 A local enlarged view of region B of the sequencing device of an embodiment of the present application.

[0073] Fig.14 A schematic diagram of the structure of the temperature control unit and the support unit of the sequencing device according to an embodiment of the present application.

[0074] For further information, see Fig.13 and Fig.14 The sequencing device 200 further includes a temperature control unit 5, which is located in the opening 11 and is used to increase or decrease the temperature of the sequencing chip, thereby achieving the purpose of controlling the temperature of the sequencing chip. Preferably, the temperature control unit 5 is used to decrease the temperature of the sequencing chip.

[0075] The temperature control unit 5 is used to detect and control the temperature of the sequencing chip. The temperature control unit 5 can heat and cool the sequencing chip to keep it in a good temperature environment, thereby improving the sequencing accuracy of the sequencing device 200 of the embodiment of the present application.

[0076] Further, see Fig.13 and Fig.14The sequencing device 200 further includes an operating unit 6 , which is disposed on the base 1 and connected to the sample loading unit 8 .

[0077] When the sequencing device 200 of the embodiment of the present application performs gene sequencing, the sequencing chip will be placed on the sample loading part 8 and connected to the electrical connection part of the sample loading part 8; after the gene sequencing is completed, the sequencing chip needs to be removed. The sample loading part 8 and the sequencing chip are detachably connected, such as by snapping. The operating part 6 can release the connection between the sample loading part 8 and the sequencing chip. For example, the operating part 6 can be a physical button or a virtual button.

[0078] The drive control unit 41 may be integrated in the base 1, and the sequencing start signal and the sequencing end signal of the sequencing device 200 of the embodiment of the present application are received by the drive control unit 41. Exemplarily, the drive control unit 41 may adopt an integrated printed circuit board (PABC).

[0079] Furthermore, the sequencing device 200 of the embodiment of the present application also includes a main control component, which is disposed in the base 1 and is used to control sequencing of the sequencing chip. Exemplarily, the main control component can adopt an integrated printed circuit board (PABC).

[0080] The present application also provides a sequencing system, including: the sequencing device 200 of the previous embodiment of the present application; a host 100, which is electrically connected to the drive control unit 41, and the host 100 is used to send an operation instruction to the drive control unit. Optionally, the host 100 can also send an operation instruction to the main control component to control the operation of the main control component.

[0081] The operation instruction may include a sequencing start signal and / or a sequencing end signal. The user sends a sequencing start signal and / or a sequencing end signal to the sequencing system through the host 100, and the sequencing device 200 receives the sequencing start signal and / or the sequencing end signal to start gene sequencing and / or end gene sequencing. Exemplarily, the host 100 may be a computer or a workstation.

[0082] In summary, the embodiments of the present application provide a sequencing device and a sequencing system, wherein the top cover body of the top cover assembly is movably connected to the base, and the top cover driving unit can drive the top cover body to move relative to the base, so that the opening of the base is exposed, or the top cover body covers the opening, thereby realizing the automatic opening and closing of the sequencing device of the embodiments of the present application; the injection assembly can suck the sequencing sample into the sequencing chip, and the injection driving unit can control the rotary valve to open or close, thereby realizing the automatic start and stop of injection of the sequencing device of the embodiments of the present application; the drive control unit can control the top cover driving unit and the injection driving unit, so through the drive control unit, the sequencing device of the embodiments of the application can improve the degree of automation of the sequencing process, reduce the influence of human factors, and improve the accuracy and reliability of sequencing.

[0083] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in the present application, and these modifications or replacements should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A sequencing device, characterized in that: include: A base having an opening, wherein the opening is located on a surface of the base perpendicular to a first direction; A sample loading portion, at least partially located in the opening, for carrying a sequencing chip; A top cover assembly, comprising a top cover body, the top cover body being movably connected to the base, and the top cover body being used to cover or expose the opening; A liquid injection assembly, at least partially located in the base, the liquid injection assembly includes a rotary valve and a pipeline system, the rotary valve is arranged in the pipeline system; The driving assembly includes a driving control unit, a top cover driving unit and a liquid injection driving unit. The driving control unit is arranged in the base and is used to control the operation of the top cover driving unit and / or the liquid injection driving unit. The top cover driving unit is used to drive the top cover body to move relative to the base, so as to cover or expose the opening; the liquid injection driving unit is used to drive the liquid injection assembly to inject liquid.

2. The sequencing device according to claim 1, characterized in that The injection assembly also includes an injection pump, which is connected to the pipeline system, and the injection drive unit is used to drive the injection pump to inject liquid into the sequencing chip.

3. The sequencing device according to claim 1 or 2, characterized in that: The liquid injection driving unit is used to control the rotation of the rotary valve, thereby opening or closing the pipeline system.

4. The sequencing device according to claim 1, characterized in that: The top cover assembly also includes a first rotating shaft and a second rotating shaft, the first rotating shaft and the second rotating shaft are respectively connected to the two ends of the top cover body along the second direction; the second rotating shaft is rotatably connected to the top cover driving part; the base is provided with a rolling groove extending along the second direction, and the first rotating shaft is movably arranged in the rolling groove.

5. The sequencing device according to claim 4, characterized in that: The top cover driving part includes a driving motor, a transmission wheel group, a transmission shaft, and a rocker arm. The driving motor is arranged in the base, the driving motor is power-connected to the transmission wheel group, the transmission wheel group is power-connected to the transmission shaft, the transmission shaft is rotatably connected to the base, one end of the rocker arm is fixedly connected to the transmission shaft, and the other end of the rocker arm is rotatably connected to the second rotating shaft.

6. The sequencing device according to claim 5, characterized in that: The top cover driving unit further includes a photoelectric switch, and the photoelectric switch is arranged on the transmission shaft.

7. The sequencing device according to claim 1, characterized in that: The sequencing device further includes a temperature control unit, which is located in the opening and is used to increase or decrease the temperature of the sequencing chip.

8. The sequencing device according to claim 1, characterized in that: The sequencing device further includes an operating unit, which is disposed on the base and connected to the sample loading unit.

9. The sequencing device according to claim 1, characterized in that: The sequencing device also includes a main control component, which is arranged in the base and is used to control the sequencing operation of the sequencing chip.

10. A sequencing system, characterized in that: include: The sequencing device according to any one of claims 1 to 9; The host is electrically connected to the drive control unit, and the host is used to send an operation instruction to the drive control unit.

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