Gene sequencing library preparation instrument

Abstract

The utility model relates to a gene sequencing library preparation instrument, which comprises a first shell, a second shell and a third shell, the automatic door is rotationally connected to the first shell and can rotate by at least 90 degrees so as to open or close the opening; a plurality of functional devices; the material pushing device is arranged in the first shell, and the material pushing device can convey the borne consumable box out of the first shell through the opening, push the consumable box away from the material pushing device, bear the consumable box located outside the first shell and convey the consumable box located outside the first shell into the first shell; and the transfer device can transfer the consumable boxes and the solution between the multiple functional devices and the material pushing device. The transferring device can be matched with a plurality of functional devices to achieve gene sample library preparation, waste consumables and a consumable box needing to be transferred to next equipment are transferred to the pushing device, manual operation is not needed, and library building efficiency is improved. Meanwhile, the opening is opened, so that the materials enter and exit from the first shell.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a gene sequencing library preparation instrument. Background Art

[0002] The value of genomic data in clinical diagnosis and treatment is constantly being explored. Among them, gene sequencing is an important technology for analyzing genomic data, and its clinical application has been increasingly expanded and deepened.

[0003] The experimental system of gene sequencing is complex, involving multiple steps including extraction, quantification, library construction, sequencing, and analysis, and each step is basically carried out between different devices.

[0004] In the prior art, the conversion of materials between the gene sequencing library preparation instrument and the experimental links associated with the front and back ends, such as sequencing equipment, is achieved through manual operation, which leads to low detection efficiency.

[0005] In addition, in the prior art, gene sequencing library preparation instruments have a low degree of automation, low throughput, and single function. Utility Model Content

[0006] The purpose of the utility model is to provide a gene sequencing library preparation instrument to at least solve one of the above-mentioned technical problems.

[0007] To achieve the above-mentioned purpose, the utility model provides a gene sequencing library preparation instrument, comprising:

[0008] A first shell, wherein an opening is provided on a wall of the first shell;

[0009] an automatic door rotatably connected to the first housing, the automatic door being capable of rotating at least 90° to open or close the opening;

[0010] A plurality of functional devices are arranged in the first housing and can hold consumable boxes, amplification reaction samples and / or fluorescence detection;

[0011] A material pushing device is disposed in the first shell, the material pushing device can transport the carried consumable box to the outside of the first shell through the opening, and push the consumable box away from the material pushing device, and can carry the consumable box outside the first shell, and transport the consumable box outside the first shell into the first shell;

[0012] The transfer device is arranged in the first shell and can transfer the consumable box and the solution between the plurality of the functional devices and the pushing device.

[0013] Optionally, the automatic door comprises a door frame, a door body, a door connector, a fixed adjustment component and a door drive mechanism;

[0014] The door frame is connected to the inner side of the first shell, the door frame is an annular structure, and the inner edge of the door frame is opposite to the opening;

[0015] The door connecting member comprises a first connecting portion, a second connecting portion and a third connecting portion which are connected in sequence in a U shape, one end of the first connecting portion is connected to the inner side of the door body through the fixed adjustment component, the fixed adjustment component can fine-tune its relative position with the door body, and is detachably connected to the inner side of the door body;

[0016] The door driving mechanism is connected to one end of the third connecting portion, so that the door body is located in a closed position abutting against the inner edge and an open position opening the opening, and when the door body is located in the closed position, the door body is located in the opening;

[0017] The lower side frame of the door frame is provided with an avoidance groove, and when the door body is located in the open position, the third connection part is at least partially located in the avoidance groove; the first connection part, the second connection part and the third connection part form an avoidance space for avoiding the lower side frame.

[0018] Since one end of the first connecting part is connected to the inner side of the door body through a fixed adjusting component, the fixed adjusting component can fine-tune its relative position with the door body. Therefore, when the door body is in a closed position, or due to processing errors or installation errors, the door body cannot fit the inner edge of the door frame, the position of the door body can be fine-tuned by adjusting the connection position between the door body and the fixed adjusting component, so that the door body can completely fit the inner edge of the door frame, thereby avoiding the situation where the door body is not closed tightly.

[0019] Since the door frame is connected to the inner side of the first shell, the door frame is annular in structure, and the inner edge of the door frame is opposite to the opening, when the door body closes the opening, the door body fits to the inner edge of the door frame, and the door frame and the door body cooperate to completely close the opening. The door body is located in the opening, that is, the door body is embedded in the box wall, and the door body does not protrude from the box wall, making the integrity of the first shell stronger.

[0020] The door driving mechanism is connected to one end of the third connecting part, and the door body and the door connecting member rotate around one end of the third connecting part to close and open the opening. Therefore, the automatic door is connected to the first housing by rotation, and only one set of door driving mechanisms is needed to drive the door body to close and open the opening, thereby simplifying the structure of the gene sequencing library preparation instrument, reducing the space occupied by the automatic door, and reducing the cost of the automatic door.

[0021] The first connection part, the second connection part and the third connection part form an escape space for escaping the lower frame, so that the door connection member will not interfere with the box wall and the door frame when the door body rotates between the open position and the closed position, and will not interfere with the conveyed materials when the door body is in the open position. In addition, since the door body and the door connection member rotate around one end of the third connection part, the door body is at a distance from one end of the third connection part. Therefore, in the process of switching the door body from the closed position to the open position, the door body will gradually move away from the box wall. Therefore, the door body will not interfere with the box wall, so that the door body can be closed smoothly.

[0022] The lower frame of the door frame is provided with an avoidance groove, and when the door body is in the open position, the third connection part is at least partially located in the avoidance groove. Therefore, when the automatic door is installed, the automatic door as a whole can be closer to the box wall, saving space inside the detection box, making the automatic door occupy less space and the structure of the detection box more compact. At the same time, it can also ensure that the strength of the door connection piece is not affected.

[0023] The automatic door can prevent dust and the like from entering the first shell from the opening. The structure of the automatic door reduces the space occupied by the automatic door, reduces costs, ensures that the opening of the door body and the opening of the door body when closed do not protrude from the first shell, making the first shell have a better overall feel.

[0024] Optionally, the fixed adjustment component includes:

[0025] A fixing bar, wherein at least one end of the fixing bar is provided with a U-shaped fixing groove;

[0026] A fixing screw, the fixing screw being fixedly connected to the inner side of the door body, the fixing screw being passed through the U-shaped fixing groove and being movable relative to the U-shaped fixing groove;

[0027] A fixing nut, the fixing nut being threadably connected to the fixing screw rod so that the fixing strip is sandwiched between the door body and the fixing nut;

[0028] The adjusting gasket is multiple in number and can be selectively arranged between the fixing nut and the fixing strip, and the adjusting gasket is sleeved on the fixing screw.

[0029] Optionally, when the door body is in the closed position, the door body is arranged vertically; and / or

[0030] When the door body is located at the open position, the door body is arranged horizontally.

[0031] Optionally, a side of the door frame close to the door body is an abutment surface, and an abutment groove is provided on the outer edge of the abutment surface;

[0032] The box wall includes a metal frame and a transparent plate, the metal frame is provided with a perspective window, and the transparent plate is connected to the inner side of the metal frame and covers the perspective window;

[0033] The door body and the metal frame abut on the abutting surface, the transparent plate is arranged in the abutting groove and abuts against the bottom of the abutting groove; and / or

[0034] The inner edge is connected with an elastic sealing portion, and the door body can be closely attached to the elastic sealing portion.

[0035] Optionally, the pushing device comprises:

[0036] A material pushing base, connected to the first shell, on which a material pushing slide rail and a material pushing drive assembly are provided;

[0037] A supporting platform, slidably connected to the push rail, for supporting the consumables box;

[0038] The push drive assembly drives the supporting platform to move along the push slide rail through the push transmission assembly, so that the supporting platform enters and exits the first housing through the opening;

[0039] The material pushing assembly is connected to the supporting platform and is used to push the consumable box away from the supporting platform.

[0040] The consumable box on the supporting platform is pushed away from the supporting platform, so that the pushing device can complete the docking of the consumable box more quickly, thereby improving the docking efficiency of the consumable box of the gene sequencing library preparation instrument.

[0041] Optionally, the pushing assembly includes an electric push rod and a pushing head, and the pushing head is connected to the output end of the electric push rod.

[0042] Optionally, the supporting platform is provided with a strip hole, and the strip hole is parallel to the electric push rod; the electric push rod is connected below the supporting platform, and the push head passes through the strip hole from below and extends out to the upper surface of the supporting platform.

[0043] Optionally, the plurality of functional devices include:

[0044] a cooling device capable of carrying the consumable box and cooling the consumable box; and / or

[0045] a heating device capable of carrying the consumable box and heating the consumable box; and / or

[0046] A normal temperature board module capable of carrying the consumable box at normal temperature; and / or

[0047] A recycling module capable of recycling discarded consumables; and / or

[0048] a calibration device on which the position of the consumables box can be calibrated; and / or

[0049] A shaking device to mix the solution; and / or

[0050] A magnetic rack device capable of enriching magnetic microspheres by magnetic force; and / or

[0051] PCR amplification device, used to amplify reaction samples; and / or

[0052] The fluorescence detection device is used to detect the reaction samples after amplification.

[0053] Optionally, the cooling device comprises:

[0054] A cooling module, comprising a cooling base connected to the first housing and a cooling water row detachably arranged above the cooling base, wherein the cooling water row is used to cool the consumable box;

[0055] The condensation water barrier module includes a cover support frame, a first cover plate, a second cover plate and a cover drive unit, wherein the cover support frame is provided with a cooling shaft connected to the first cover plate; the first cover plate is fixedly connected to the second cover plate through a cover elastic member, and the cover drive unit is connected to the cover support frame, and is used to drive the cooling shaft to rotate so that the second cover plate seals and covers the consumable box or opens the consumable box; the cooling process is accompanied by the condensation water barrier module to isolate the consumable box and the consumable box from the air, thereby preventing the formation of condensation water. At the same time, since the second cover plate can open and cover the consumable box, the condensation process and the consumable box handling process can be carried out continuously, thereby ensuring the accuracy and continuity of the test.

[0056] and / or

[0057] The heating device comprises:

[0058] A heating base is connected to the first shell and is provided with a sink;

[0059] A heating plate is arranged in the sink of the heating base;

[0060] A heating jig is arranged on the heating base, a heating groove is provided on the upper surface of the heating jig, the consumable box is placed on the heating jig, a containing unit of the consumable box can be inserted into the heating groove and contact with the groove wall of the heating groove, and a breathable structure is provided on the groove wall of the heating groove; the heating jig increases the contact area with the heated consumable box and provides a breathable structure, thereby solving the problem of inconvenience in taking and placing the consumable box due to the adhesion between the heating jig and the lower surface of the consumable box, and achieving the effect of conveniently taking and placing the consumable box on the heating jig.

[0061] and / or

[0062] The calibration device comprises:

[0063] A placement surface, wherein an upper portion of the placement surface defines a placement area;

[0064] A first calibration fixing block, located at a first calibration side of the placement area in a first direction;

[0065] A first calibration movable block is located at a second calibration side of the placement area in a first direction, the first calibration movable block is configured to be movable along the first direction, wherein the second calibration side in the first direction is arranged opposite to the first calibration side in the first direction;

[0066] a second calibration fixing block, the second calibration fixing block being located at a first calibration side of a second direction of the placement area, wherein the first direction and the second direction are perpendicular to each other; and

[0067] a second calibration movable block, the second calibration movable block being located at a second calibration side of the placement area in a second direction, the second calibration movable block being configured to be movable along the second direction, wherein the second calibration side in the second direction is arranged opposite to the first calibration side in the second direction; and the first direction is perpendicular to the second direction;

[0068] and / or

[0069] The oscillating device comprises:

[0070] An oscillating working support, connected to the first housing;

[0071] An oscillating motor assembly and an oscillating eccentric wheel assembly, wherein the oscillating motor assembly comprises an oscillating stepping motor and a coupling assembly, and the oscillating stepping motor is installed on the oscillating working bracket;

[0072] The output end of the oscillating stepper motor is eccentrically arranged through the oscillating working bracket and the oscillating eccentric wheel assembly, and both are connected to the coupling assembly;

[0073] An oscillating tray, connected to the oscillating eccentric wheel assembly, and the oscillating eccentric wheel assembly can rotate relative to the oscillating tray, and a detachable consumable box is placed on the oscillating tray;

[0074] An oscillation guide mechanism is disposed between the oscillation working support and the oscillation tray, the oscillation guide mechanism is located on one side of the oscillation eccentric wheel assembly, and the oscillation guide mechanism limits the oscillation tray to move in a first direction and a second direction in a horizontal plane;

[0075] A reset assembly, comprising a first fixing member, an oscillation reset spring and a crank connecting rod member, wherein the first fixing member is arranged on the oscillation working support or at the bottom of the oscillation tray;

[0076] A crank connecting rod member connected to the coupling assembly and capable of rotating relative to the coupling assembly, wherein the rotation center of the crank connecting rod member is not coaxial with the oscillating stepping motor and / or the coupling assembly;

[0077] One end of the oscillation return spring is connected to the crank connecting rod, and the other end is connected to the first fixing member. The oscillation stepping motor rotates to drive the oscillation eccentric wheel assembly to move through the coupling assembly, thereby driving the oscillation tray connected to the oscillation eccentric wheel assembly to move in the horizontal plane along the first direction and the second direction to shake the consumable box, so that the solution therein is oscillated evenly.

[0078] In addition, the above-mentioned consumable box can be quickly disassembled and placed on the shaking tray, and the consumable box containing the mixed reagent is placed on the consumable box, and the consumable box is removed synchronously after the shaking is completed. When the shaking device is working, the consumable box can be placed on the consumable box for shaking, and the consumable box and the consumable box are moved to the shaking tray at the same time, thereby improving work efficiency.

[0079] At the same time, when the oscillation stepper motor drives the oscillation tray to move in the horizontal plane, the oscillation reset spring is stretched. When the oscillation stepper motor stops working, the oscillation reset spring contracts, pulling the oscillation stepper motor to reset, so that the consumable box on the oscillation tray returns to the origin, making it easier for the automation system to identify the position of the consumable box, so that the oscillation device can be applied to the automation system.

[0080] Optionally, the heating fixture includes a plurality of vertical bodies, at least two of the vertical bodies enclose the heating groove, and a gap exists between two adjacent vertical bodies without the accommodating monomer inserted therein to form a breathable structure.

[0081] Optionally, the calibration device further includes:

[0082] A calibration drive mechanism, wherein the calibration drive mechanism is configured to drive the first calibration movable block and the second calibration movable block to approach or move away from the placement area at the same time, and the calibration drive mechanism comprises:

[0083] A lifting platform, the lifting platform comprising:

[0084] a first calibration slope, the first calibration slope facing the first direction, the first calibration slope connected to the first calibration movable block; and

[0085] a second calibration bevel, the second calibration bevel facing the second direction, and the second calibration bevel connected to the second calibration movable block;

[0086] Wherein, the lifting platform has a high position and a low position;

[0087] Wherein, when the lifting platform is located at the low position, the first calibration movable block is connected to the upper part of the first calibration inclined surface, the second calibration movable block is connected to the upper part of the second calibration inclined surface, and the first calibration movable block and the second calibration movable block are both located at a calibration position relatively close to the placement area;

[0088] Wherein, when the lifting platform is in the high position, the first calibration movable block is connected to the lower part of the first calibration inclined plane, and the second calibration movable block is connected to the lower part of the second calibration inclined plane, and the first calibration movable block and the second calibration movable block are both located in a non-calibration position relatively away from the placement area.

[0089] Optionally, the gene sequencing library preparation instrument further includes an anti-lifting device, the cooling device, the heating device and / or the normal temperature plate module are connected with the anti-lifting device, and the anti-lifting device is used to fix the consumable box located on the cooling device, the heating device or the normal temperature plate module;

[0090] The anti-lifting device comprises:

[0091] A clamping and fixing component, which is fixedly arranged relative to the cooling device, the heating device or the normal temperature plate module;

[0092] A clamping and lifting assembly, comprising a clamping and lifting seat, wherein the clamping and lifting seat can be lifted and lowered relative to the clamping and fixing assembly;

[0093] A clamping linkage assembly, comprising a clamping abutment, a clamping linkage and a clamping elastic member, wherein the clamping abutment abuts against the clamping lifting seat, the clamping abutment is connected to the clamping lifting seat via the clamping elastic member, and one end of the clamping linkage is rotatably connected to the clamping abutment;

[0094] A clamping assembly, which includes a clamping jaw, wherein the clamping jaws are symmetrically arranged on two opposite sides of the consumable box along the second direction, the clamping jaws are arranged in a one-to-one correspondence with the clamping linkage assembly, and the other end of the clamping linkage is coaxially fixedly connected with the clamping jaw, and each of the clamping jaws can be rotatably arranged on the cooling device, the heating device or the constant temperature plate position module to be able to clamp the consumable box so that the consumable box is pressed tightly against the cooling device, the heating device or the constant temperature plate position module. The clamping lifting seat rises, and because the clamping abutment abuts against the clamping lifting seat, the clamping lifting seat drives the clamping abutment to move up, and the clamping abutment makes the clamping linkage rotate around the fixed axis, thereby driving the clamping claws to rotate. Since the clamping claws are symmetrically arranged on the opposite sides of the consumable box, the rotation directions of the two clamping claws are opposite, and the two clamping claws are in an open state to facilitate the taking and placing of the consumable box. During the fixed axis rotation of the clamping linkage, the clamping abutment will be driven to roll to the right along the second direction on the clamping lifting seat, and the clamping abutment will The clamping elastic member is driven to extend; the clamping lifting seat descends, and the elastic deformation of the clamping elastic member forces the clamping abutment to follow the clamping lifting seat to descend, and the clamping abutment rolls leftward along the second direction on the clamping lifting seat, thereby causing the clamping linkage to rotate in the opposite direction around the fixed axis, and the clamping linkage drives the clamping claws to rotate in the opposite direction around the fixed axis, so that the two clamping claws are in a clamping state, that is, the two clamping claws clamp the consumable box, which can prevent the consumable box from being lifted up by the pipette gun, prevent damage to the consumable box, ensure normal pipetting, and improve library construction efficiency.

[0095] Optionally, the normal temperature panel module includes a first normal temperature panel module, a plurality of second normal temperature panel modules and a third normal temperature panel module;

[0096] The cooling device, the first constant temperature panel module, the heating device and at least one of the second constant temperature panel modules are arranged in sequence along the second direction to form a first column of functional areas;

[0097] The PCR amplification device, the magnetic frame device and the recovery module are sequentially arranged along a second direction to form a second column of functional areas;

[0098] The fluorescence detection device and at least one of the second constant temperature panel modules are arranged in sequence along the second direction to form a third column of functional areas;

[0099] At least one of the second normal temperature plate position modules, the oscillation device and the calibration device are sequentially arranged along the second direction to form a fourth column of functional areas;

[0100] The pushing device and the third normal temperature plate position module are arranged in sequence along the second direction to form a fifth column of functional areas;

[0101] The first column of functional areas, the second column of functional areas, the third column of functional areas, the fourth column of functional areas and the fifth column of functional areas are sequentially arranged along a first direction.

[0102] Optionally, the transfer device comprises:

[0103] A clamping and placing mechanism, capable of clamping and placing the consumables box;

[0104] A pipetting mechanism capable of transferring the solution in the consumable box;

[0105] A three-way driving mechanism is connected to the top of the first housing, and is used to drive the clamping and placing mechanism and the liquid transfer mechanism to move in the horizontal direction and the vertical direction. BRIEF DESCRIPTION OF THE DRAWINGS

[0106] Figure 1 It is a structural schematic diagram of an opening of a gene sequencing library preparation instrument closed by an automatic door provided in an embodiment of the utility model;

[0107] Figure 2 It is a structural schematic diagram of a gene sequencing library preparation instrument provided by an embodiment of the utility model with a portion of the first housing removed;

[0108] Figure 3 It is a schematic diagram of the structure of the transfer device provided by the embodiment of the utility model;

[0109] Figure 4 yes Figure 2 AA section view;

[0110] Figure 5 It is a schematic diagram of the structure of the cooling device provided by the embodiment of the utility model;

[0111] Figure 6 It is a schematic diagram of the structure of a cooling module in a cooling device provided in an embodiment of the utility model;

[0112] Figure 7 It is a structural schematic diagram of a fixing unit in a cooling device provided by an embodiment of the utility model;

[0113] Figure 8 It is a schematic diagram of the structure of the cooling water row in the cooling device provided by the embodiment of the utility model;

[0114] Fig. 9 It is a structural schematic diagram of a condensation water barrier module in a cooling device provided by an embodiment of the utility model;

[0115] Fig.10 It is a structural schematic diagram of a heating device provided in an embodiment of the utility model;

[0116] Fig.11 yes Fig.10A partial enlarged view of the middle A;

[0117] Fig.12 This is a schematic diagram of the structure of the heating device provided by the embodiment of the utility model without a heating fixture placed therein;

[0118] Fig.13 It is a schematic structural diagram of the heating device provided by the embodiment of the utility model without the heating sheet and the heat insulation sheet;

[0119] Fig.14 It is a structural schematic diagram of the connection between the cooling device, the heating device or the normal temperature panel module and the anti-lifting device provided in an embodiment of the utility model;

[0120] Fig.15 This is a front view of the anti-belt lifting device provided by the embodiment of the utility model when it is opened;

[0121] Fig.16 This is a front view of the anti-belt lifting device provided by the embodiment of the utility model when it is clamped;

[0122] Fig.17 The present invention provides a schematic diagram of the cooling device, the heating device or the partial structure of the normal temperature plate module and the anti-lifting device. Figure 1 ;

[0123] Fig.18 yes Fig.14 A partial enlarged view of point B in the middle;

[0124] Fig.19 yes Fig.14 A partial enlarged view of point C in the middle;

[0125] Fig. 20 The present invention provides a schematic diagram of the cooling device, the heating device or the partial structure of the normal temperature plate module and the anti-lifting device. Figure 2 ;

[0126] Fig.21a It is a structural schematic diagram of a first angle of the oscillation device provided by an embodiment of the utility model;

[0127] Figure 21b It is a structural schematic diagram of a second angle of the oscillation device provided by an embodiment of the utility model;

[0128] Fig. 22 It is a structural schematic diagram of the oscillation device provided in the embodiment of the utility model (excluding the oscillation tray);

[0129] Fig.23 It is a structural schematic diagram of a coupling assembly provided by an embodiment of the utility model;

[0130] Fig.24aIt is a front view of the coupling assembly provided by the embodiment of the utility model;

[0131] Figure 24b The utility model embodiment provides Fig.24a BB section view;

[0132] Fig.25a It is a structural schematic diagram of a first angle of the oscillation guide mechanism provided by an embodiment of the utility model;

[0133] Fig.25b It is a structural schematic diagram of a second angle of the oscillation guide mechanism provided by an embodiment of the utility model;

[0134] Fig.26a It is a structural schematic diagram of a first angle of a first guide connecting member provided by an embodiment of the utility model;

[0135] Figure 26b It is a structural schematic diagram of a second angle of the first guide connecting member provided in an embodiment of the utility model;

[0136] Fig.27a It is a structural schematic diagram of a first angle of the oscillation tray provided in an embodiment of the utility model;

[0137] Figure 27b It is a structural schematic diagram of a second angle of the oscillation tray provided in an embodiment of the utility model;

[0138] Fig.28 is a schematic diagram of a calibration device provided by an embodiment of the utility model;

[0139] Fig.29 It is a schematic diagram of a calibration device provided by an embodiment of the utility model after the second housing is removed from one viewing angle;

[0140] Fig.30 is a schematic diagram of another viewing angle of the calibration device after the second housing is removed provided by an embodiment of the utility model;

[0141] Fig.31 is a schematic diagram of a calibration drive mechanism provided in an embodiment of the utility model;

[0142] Fig.32 It is a schematic diagram of a lifting platform, a first calibration cam follower and a first calibration movable block provided in an embodiment of the utility model;

[0143] Fig.33 It is a schematic diagram of a lifting platform, a second calibration cam follower and a second calibration movable block provided in an embodiment of the utility model;

[0144] Fig.34 It is a structural schematic diagram of the extended state of the pusher device provided in the embodiment of the utility model;

[0145] Fig.35 It is a structural schematic diagram of the retracted state of the pusher device provided by the embodiment of the utility model;

[0146] Fig.36 It is a structural schematic diagram of an opening of a gene sequencing library preparation instrument provided by an embodiment of the utility model through an automatic door;

[0147] Fig.37 yes Fig.36 A partial enlarged view of point D in the middle;

[0148] Fig.38 It is a structural schematic diagram of the connection between the box wall and the automatic door (the door body is in the open position) provided by an embodiment of the utility model;

[0149] Fig.39 yes Fig.38 A partial enlarged view of point E in the middle;

[0150] Fig.40 It is a structural schematic diagram of the connection between the fixing strip and the door connecting piece provided by the embodiment of the utility model;

[0151] Fig.41 It is a structural schematic diagram of an automatic door (the door body is in a closed position) provided in an embodiment of the utility model;

[0152] Fig.42 It is a partial structural schematic diagram of an automatic door (the door body is in a closed position) provided by an embodiment of the utility model;

[0153] Fig.43 It is a partial structural schematic diagram of an automatic door (door body is in the open position) provided by an embodiment of the utility model;

[0154] Fig.44 It is a structural schematic diagram of a door frame provided by an embodiment of the utility model;

[0155] Fig.45 It is a structural schematic diagram of a door frame provided by an embodiment of the utility model from another perspective;

[0156] Fig.46 It is a cross-sectional view of the box wall and the door body provided by the embodiment of the utility model;

[0157] Fig.47 yes Fig.46 A partial enlarged view of point F in the middle.

[0158] The beneficial effects of the utility model are:

[0159] The transfer device can transfer consumable boxes and solutions between multiple functional devices and the pushing device, so that the preparation of the gene sample library can be realized in conjunction with multiple functional devices. After the preparation of the gene sample library is completed, the transfer device can transfer the discarded consumables and the consumable boxes that need to be transferred to the next device to the pushing device, and the automatic door will open the opening. The pushing device transfers the discarded consumable boxes and the consumable boxes that need to be transferred to the outside of the first shell through the opening, so that the consumable boxes and other materials are transferred through the gene sequencing library preparation instrument, and no manual operation is required, thereby improving the efficiency of library construction. The automatic door can rotate at least 90°, so that the automatic door will not interfere with the pushing device after opening the first shell. Through the setting of the automatic door, multiple functional devices, the pushing device and the transfer device, no manual operation is required during the preparation of the gene sequencing library, and the degree of automation is high.

[0160] In the figure:

[0161] 1. Heating device; 2. Anti-lifting device; 3. Automatic door; 4. Pushing device; 5. Cooling device; 6. Transfer device; 7. Calibration device; 8. Oscillating device; 100. First shell; 101. Box wall; 1011. Metal frame; 1012. Transparent plate; 1013. Perspective window; 1014. Opening; 300. Recovery module; 400. Magnetic rack device; 500. PCR amplification device; 600. Fluorescence detection device; 200. Consumables box; 201. Box body; 202. Consumables flange;

[0162] 11. Heating base; 111. Insulation positioning column; 12. Heating plate; 13. Heating fixture; 131. Vertical body; 1311. Heating arc surface; 132. Heating groove; 133. Breathable structure; 14. Insulation plate; 141. Insulation positioning hole; 16. Heating bracket; 17. Heating fixing seat;

[0163] 21. Clamping and fixing assembly; 22. Clamping assembly; 23. Clamping and lifting assembly; 24. Clamping linkage assembly; 25. Clamping guide assembly; 26. Clamping and detecting assembly; 211. Clamping and fixing base; 212. Clamping and fixing stand; 221. Clamping claw; 222. Clamping shaft; 231. Clamping and lifting seat; 232. Clamping drive source; 233. Clamping drive seat; 234. Clamping mounting seat; 241. Clamping holding abutment; 242, clamping linkage; 243, clamping elastic member; 251, clamping guide block; 252, clamping guide rail; 261, clamping sensor; 262, clamping baffle; 2211, clamping rotating part; 2212, clamping part; 2213, clamping connecting part; 2311, clamping abutment part; 2312, clamping mounting part; 2321, clamping output shaft; 2322, motor body; 31, door frame; 311, lower frame; 3111, avoidance groove; 312, inner edge; 313, abutment surface; 314, abutment groove; 315, upper frame; 316, first side frame; 317, second side frame; 32, door body; 33, door connecting member; 331, first connecting part; 332, second connecting part; 333, third connecting part; 334, avoidance space; 34, fixed adjustment component; 341, fixing strip; 3411, U-shaped fixing groove; 342, fixing screw; 343, fixing nut; 35, door drive mechanism; 351, door drive member; 352, door shaft; 353, first fixing part of door drive; 354, second fixing part of door drive; 355, door drive driving pulley; 356, door drive driven pulley; 357, door drive belt; 36, door in place detection member; 361, door in place photoelectric sensor; 362, door stopper;

[0164] 41. Pushing base; 411. Pushing slide rail; 43. Supporting platform; 431. Strip hole; 432. Pushing slide block; 434. Limiting protrusion; 44. Pushing drive assembly; 45. Pushing transmission assembly; 46. Pushing assembly; 461. Electric push rod; 462. Pushing head; 471. Position sensor; 472. Positioning piece;

[0165] 51. Cooling module; 52. Cooling base; 53. Cooling water row; 54. Condensation water blocking module; 55. Cover support frame; 56. First cover; 57. Cooling shaft; 58. Cover elastic member; 59. Second cover; 511. Water tank; 512. Cooling water row; 513. Cover synchronous belt; 514. Condensation water hole; 515. Semiconductor refrigeration sheet; 516. Cover driving pulley; 517. Cover driven pulley; 518. Cover driving unit; 519. Cooling bracket;

[0166] 61. Three-way driving mechanism; 611. First-way driving assembly; 612. Second-way driving assembly; 613. Third-way driving assembly; 62. Clamping and placing mechanism; 63. Liquid transfer mechanism;

[0167] 71, second housing; 711, placement surface; 712, first calibration fixed block; 713, second calibration fixed block; 714, first calibration slot; 715, second calibration slot; 72, first calibration movable block; 73, second calibration movable block; 74, first calibration mounting seat; 741, first calibration slider; 742, first calibration guide rail; 743, first calibration cam follower; 75, second calibration mounting seat; 751, second calibration slider; 752, second calibration guide rail; 753, second calibration cam follower; 76, calibration Driving mechanism; 761, fixed seat; 762, third calibration driving assembly; 763, lifting platform; 7631, first calibration inclined plane; 7632, second calibration inclined plane; 764, third calibration guide rail; 77, first calibration elastic element; 78, second calibration elastic element; 81, oscillating working bracket; 811, oscillating supporting frame; 812, oscillating mounting plate; 8122, weight reduction groove; 82, oscillating motor assembly; 823, oscillating stepping motor; 824, coupling assembly; 8241, first coupling member; 8242, second coupling 8243, oscillation connecting shaft; 83, oscillation eccentric wheel assembly; 831, oscillation cam follower; 84, oscillation guide mechanism; 841, first oscillation slide rail; 842, first oscillation slider; 843, second oscillation slide rail; 844, second oscillation slider; 845, guide connection assembly; 8451, first guide connection piece; 84511, first oscillation slider installation groove; 84512, first slider fastening hole; 84513, fastening installation groove; 8452, second guide connection piece; 8453, fastening connection piece; 85, Oscillation tray; 851, tray bottom plate; 852, tray limiter; 853, plunger spring; 854, tray support plate; 86, reset assembly; 863, first fixing member; 864, oscillation reset spring; 865, crank-connecting rod member; 8651, first connecting member; 8652, second connecting member; 8653, crank; 866, second fixing member; 88, buffer member; 91, first normal temperature plate position module; 911, normal temperature bracket; 912, normal temperature base; 92, second normal temperature plate position module; 93, third normal temperature plate position module. DETAILED DESCRIPTION

[0168] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0169] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0170] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0171] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0172] like Figure 1-Figure 47As shown, the gene sequencing library preparation instrument provided in this embodiment includes a first housing 100, an automatic door 3, multiple functional devices, a material pushing device 4 and a transfer device 6, wherein the multiple functional devices, the material pushing device 4 and the transfer device 6 are all arranged in the first housing 100, an opening 1014 is arranged on the box wall 101 of the first housing 100, the automatic door 3 is rotatably connected to the first housing 100, and the automatic door 3 can be rotated at least 90° to open or close the opening 1014. The automatic door 3 can be rotated at least 90°, so that the automatic door 3 will not interfere with the material pushing device 4 after opening the first housing 100. Multiple functional devices can place consumable boxes 200, amplification reaction samples and / or fluorescence detection. The pushing device 4 can transport the carried consumable box 200 to the outside of the first housing 100 through the opening 1014, and push the consumable box 200 away from the pushing device 4, and can carry the consumable box 200 located outside the first housing 100, and transport the consumable box 200 outside the first housing 100 to the inside of the first housing 100. The transfer device 6 can transfer the consumable box 200 and the solution between multiple functional devices and the pushing device 4, so that the preparation of the gene sample library can be achieved in conjunction with multiple functional devices. After the preparation of the gene sample library is completed, the transfer device 6 can transfer the discarded consumables and the consumable box 200 that needs to be transferred to the next device to the pushing device 4, the automatic door 3 opens the opening 1014, and the pushing device 4 transfers the discarded consumable box 200 and the consumable box 200 that needs to be transferred to the outside of the first housing 100 through the opening 1014, so that the consumable box 200 and other materials are transferred through the gene sequencing library preparation instrument, without the need for manual operation, thereby improving the efficiency of library construction. The automatic door 3 closing the opening 1014 can effectively prevent dust and prevent the aerosol in the first housing 100 from escaping and polluting the environment.

[0173] In this embodiment, by providing the automatic door 3, multiple functional devices, the pushing device 4 and the transfer device, no manual operation is required during the preparation of the gene sequencing library, and the degree of automation is high.

[0174] In the present embodiment, the consumable box 200 can be used to contain solutions, and can also contain consumables such as gun tips. The upper surface of the consumable box 200 usually has a plurality of regularly arranged (e.g., matrix arranged) containing monomers, and the solution or consumables are contained in the containing monomers. The shape of the consumable box 200 can be rectangular. The consumable box 200 can have a variety of different specifications. For example, the consumable box 200 can have different numbers of containing monomers, such as 10 containing monomers, 20 containing monomers, and the like. The consumable box 200 is, for example, a deep well plate, a test tube rack, a gun tip box, a PCR plate, a fragment analyzer plate, an ELISA plate, and the like. The solution can be a reagent, a sample, and a reaction sample formed by a mixture of a reagent and a sample.

[0175] like Figure 3As shown, optionally, the transfer device 6 includes a clamping mechanism 62, a liquid transfer mechanism 63, and a three-way driving mechanism 61. The clamping mechanism 62 can clamp the consumable box 200, and the liquid transfer mechanism 63 can transfer the solution of the consumable box 200, so that the solution is transferred in each consumable box 200. The three-way driving mechanism 61 is connected to the top of the first shell 100, and the three-way driving mechanism 61 is used to drive the clamping mechanism 62 and the liquid transfer mechanism 63 to move in the horizontal direction and the vertical direction, so that the clamping mechanism 62 clamps the consumable box 200 at various locations in the first shell 100, and the liquid transfer mechanism 63 transfers the solution at various locations in the first shell 100.

[0176] Specifically, the horizontal direction includes a first direction X and a second direction Y. The first direction X, the second direction Y and the vertical direction (Z direction as shown in the figure) are perpendicular to each other, so that the clamping mechanism 62 and the pipetting mechanism 63 move in a three-dimensional space.

[0177] The three-way driving mechanism 61 may include a first-way driving component 611, a second-way driving component 612, and a third-way driving component 613, wherein the output end of the first-way driving component 611 is connected to the second-way driving component 612 to drive the second-way driving component 612 to move in the first direction X. The output end of the second-way driving component 612 is connected to the third-way driving component 613 to drive the third-way driving component 613 to reciprocate along the second direction Y, and the output end of the third-way driving component 613 is connected to the clamping and placing mechanism 62 and the liquid transfer mechanism 63 to drive the clamping and placing mechanism 62 and the liquid transfer mechanism 63 to move along the second direction Y. Optionally, the third-direction driving component 613 is connected to the clamping mechanism 62 through a third-direction sub-driving member, and the third-direction sub-driving member drives the clamping mechanism 62 to move in the vertical direction. When the clamping mechanism 62 is required to clamp the consumable box 200, the third-direction sub-driving member drives the clamping mechanism 62 to move downward. When the clamping mechanism 62 is not required to clamp the consumable box 200, or the liquid transfer mechanism 63 is required to transfer liquid, the third-direction sub-driving member drives the clamping mechanism 62 to move upward, thereby avoiding the liquid transfer mechanism 63. The third-direction sub-driving member can be any structure that can realize the vertical movement of the clamping mechanism 62, such as a cylinder or an oil cylinder. The first-direction driving component 611, the second-direction driving component 612 and the third-direction driving component 613 can all be pulleys and belt mechanisms driven by motors. Of course, the specific structures of the first-direction driving component 611, the second-direction driving component 612 and the third-direction driving component 613 are not limited thereto, as long as they can realize linear movement.

[0178] The clamping mechanism 62 may include a cylinder clamp to clamp the consumable box 200 .

[0179] The liquid transfer mechanism 63 can be connected to a liquid transfer gun or a similar component with a liquid transfer function to complete the liquid transfer operation. The structure and control method of the liquid transfer mechanism 63 belong to the prior art. As an example, the liquid transfer mechanism 63 can be an automatic liquid transfer workstation in the prior art.

[0180] like Figure 4 As shown, optionally, the multiple functional devices may include at least one of a cooling device 5, a heating device 1, a constant temperature plate module, a recovery module 300, a calibration device 7, a magnetic rack device 400, a PCR amplification device 500 and a fluorescence detection device 600.

[0181] The cooling device 5 can carry the consumable box 200 and can cool the consumable box 200 . For example, the consumable box 200 is at a low temperature, such as 4° C., in the cooling device 5 in order to store certain solutions that must be stored at a low temperature.

[0182] The heating device 1 can carry the consumable box 200 and can heat the consumable box 200 , such as the consumable box 200 of the heating device 1 is at a relatively high temperature, such as 70° C., to heat the solution, or store the solution at a higher temperature.

[0183] The normal temperature plate module can carry the consumable box 200 at normal temperature, so that the solution in the consumable box 200 can be transferred by the pipetting mechanism 63, or the consumable box 200 can be taken and placed by the clamping mechanism 62, or the pipetting mechanism 63 uses a pipette gun located in the consumable box 200, etc.

[0184] The recycling module 300 can recycle the discarded consumables, so that the pipetting mechanism 63 or the pick-and-place mechanism transfers the discarded consumables to the recycling module 300. Optionally, the recycling module 300 can be a container with a containing space, etc.

[0185] The calibration device 7 can calibrate the position of the consumable box 200 thereon. For example, when the consumable box 200 on the normal temperature plate position module needs to be transferred to the cooling device 5, the heating device 1, the oscillating device 8, the magnetic rack device 400, the PCR amplification device 500 or the fluorescence detection device 600, the consumable box 200 needs to be placed accurately. At this time, after the clamping mechanism 62 clamps the consumable box 200 in the normal temperature plate position module, the position of the consumable box 200 is calibrated on the calibration device 7 to place the consumable box 200 at the preset position of the cooling device 5, the heating device 1, the oscillating device 8, the magnetic rack device 400, the PCR amplification device 500 or the fluorescence detection device 600.

[0186] The oscillating device 8 can mix the solution so that the solution can fully react.

[0187] The magnetic rack device 400 can enrich magnetic microspheres by magnetic force, and the magnetic rack device 400 can be a magnetic rack device 400 in the prior art, as long as it can achieve the purpose of separating and purifying cells, proteins or nucleic acids and other substances by enriching magnetic microspheres by magnetic field. For example, the consumable box 200 located on the magnetic rack device 400, when the object to be detected is adsorbed on the magnetic microspheres, after the magnetic rack device 400 adsorbs the magnetic microspheres, the transfer device 6 can suck out the solution in the consumable box 200 (the magnetic microspheres are adsorbed to the inner wall of the consumable box 200 containing the monomer by the magnetic field of the magnetic rack device 400, so the transfer device 6 can suck out the solution except the magnetic microspheres in the consumable box 200), and then the transfer device 6 injects washing solution into the consumable box 200. Repeating the above process multiple times can achieve the purification of the object to be detected. When the purification is completed, a solvent is injected into the consumable box 200, and the object to be detected adsorbed on the magnetic microspheres is dissolved in the solvent. The consumable box 200 is placed on the magnetic rack device 400 again, and the magnetic microspheres are adsorbed to the inner wall of the consumable box 200 that contains the monomer by the magnetic field of the magnetic rack device 400. The transfer device 6 can suck out the solvent containing the object to be detected, thereby achieving separation of the object to be detected and the magnetic microspheres to obtain a purified product.

[0188] The PCR amplification device 500 is used to amplify reaction samples, which is a prior art and can be used as long as it can achieve amplification of reaction samples.

[0189] The fluorescence detection device 600 is used to detect the reaction sample after amplification. For example, the fluorescence quantitative detection device is configured to detect the reaction sample in the consumable box 200, such as the concentration of a specific target molecule. The structure and control method of the fluorescence quantitative detection device belong to the prior art. As an example, the fluorescence quantitative detection device can be a fluorescence quantitative instrument.

[0190] Alternatively, if Figure 4 As shown, the constant temperature plate position module includes a first constant temperature plate position module 91, a plurality of second constant temperature plate position modules 92 and a third constant temperature plate position module 93; illustratively, the third constant temperature plate position module 93 can be used to stack gun tip boxes, and at least part of the second constant temperature plate position modules 92 can be used to stack other types of consumable boxes 200. There are many plate position modules in the prior art, and the consumable boxes 200 are stored in a flat manner, and the consumable storage capacity is poor, resulting in low throughput of the gene sequencing library preparation instrument. In this embodiment, the second constant temperature plate position module 92 and the third constant temperature plate position module 93 can stack the consumable boxes 200, and the consumable storage capacity is strong, and the throughput of the gene sequencing library preparation instrument is high.

[0191] The cooling device 5, the first constant temperature panel module 91, the heating device 1 and at least one second constant temperature panel module 92 are sequentially arranged along the second direction Y to form a first column of functional areas; illustratively, in this embodiment, the first column of functional areas is provided with a second constant temperature panel module 92. Fig.17 As shown, illustratively, the first normal temperature plate module 91 includes a normal temperature bracket 911 and a normal temperature base 912 disposed on the normal temperature bracket 911 , the normal temperature bracket 911 is connected to the first housing 100 , and the normal temperature base 912 is used to carry the reagent kit.

[0192] The PCR amplification device 500, the magnetic rack device 400 and the recovery module 300 are sequentially arranged along the second direction Y to form a second column of functional areas;

[0193] The fluorescence detection device 600 and at least one second constant temperature panel module 92 are sequentially arranged along the second direction Y to form a third column of functional areas; illustratively, in this embodiment, three second constant temperature panel modules 92 are arranged in the third column of functional areas.

[0194] At least one second constant temperature panel module 92, the oscillation device 8 and the calibration device 7 are sequentially arranged along the second direction Y to form a fourth column of functional areas; illustratively, in this embodiment, three second constant temperature panel modules 92 are arranged in the fourth column of functional areas.

[0195] The pushing device 4 and the third normal temperature plate position module 93 are arranged in sequence along the second direction Y to form a fifth column of functional areas.

[0196] The first column of functional areas, the second column of functional areas, the third column of functional areas, the fourth column of functional areas and the fifth column of functional areas are arranged in sequence along the first direction X.

[0197] In this embodiment, the placement of each module can shorten the movement path of the transfer device 6 and improve work efficiency.

[0198] In this embodiment, by providing a fluorescence detection device 600, a calibration device 7 and an oscillation device 8, the gene sequencing library preparation instrument has the functions of detecting sample concentration, calibrating the consumable box 200 and mixing the reaction sample, thereby diversifying the functions of the gene sequencing library preparation instrument.

[0199] The specific structure of the cooling device 5 is described in detail below.

[0200] like Figure 5-Figure 9 As shown, the cooling device 5 includes a cooling module 51, such as Figure 6 and Figure 7 As shown, the cooling module 51 includes a cooling base 52 connected to the first housing 100 (the cooling base 52 may be a heat insulating board made of a heat insulating material) and a cooling water row 53 detachably disposed above the cooling base 52, and the cooling water row 53 is used to cool the consumable box 200. The cooling module 51 may also include a cooling bracket 519, and the cooling base 52 may be connected to the first housing 100 through the cooling bracket 519.

[0201] like Figure 8As shown, the cooling module 51 may further include a semiconductor cooling sheet 515, which is arranged on the top of the cooling water row 53. The cooling water row 53 cools the semiconductor cooling sheet 515, and then cools the solution in the consumable box 200 through the semiconductor cooling sheet 515. The main function of the semiconductor cooling sheet 515 is to increase the cooling speed of the consumable box 200.

[0202] In order to monitor the temperature drop of the consumable box 200, temperature sensors can be respectively set on the upper and lower surfaces of the semiconductor refrigeration plate 515, wherein the temperature sensor on the upper surface is used to detect whether the consumable box 200 has been cooled to 4°C, and the temperature sensor on the lower surface is used to detect whether the temperature is too high.

[0203] The temperature of the consumable box 200 and the solution during the cooling process ranges from 60°C to 70°C, so the cooling water discharge 53 preferably adopts liquid cooling, such as Figure 6 As shown, the cooling module 51 may also include a heat dissipation water row 512 and a water tank 511, and the cooling water row 53, the water tank 511 and the heat dissipation water row 512 are sequentially connected to form a loop. Specifically, the heat dissipation water row 512 cools the coolant, and then transports the coolant to the heat dissipation water row 512. After the coolant cools the heat dissipation water row 512, it flows back to the water tank 511, and the coolant in the water tank 511 flows back to the heat dissipation water row 512 again for heat dissipation. The water tank 511 may be connected to a water pump to serve as a power supply for water circulation, and the heat dissipation water row 512 may include a heat dissipation pipeline. When the water pump transports the liquid to the heat dissipation water row 512, the heat in the coolant will be released through the aforementioned heat dissipation pipeline.

[0204] like Figure 7 and Figure 8 As shown, during the cooling process of the consumable box 200, condensation water is easily generated on the surface of the cooling base 52 and the consumable box 200. Therefore, in order to facilitate the discharge of condensation water, a condensation water hole 514 running vertically is provided in the cooling water drain 53. When condensation water is generated, it will be discharged downward through the condensation water hole 514.

[0205] like Fig. 9 As shown, the condensation water blocking module 54 includes a cover support frame 55, a first cover 56, a second cover 59 and a cover drive unit 518. The cover support frame 55 is provided with a cooling shaft 57 connected to the first cover 56. The first cover 56 is fixedly connected to the second cover 59 through a cover elastic member 58. The cover drive unit 518 is connected to the cover support frame 55 and is used to drive the cooling shaft 57 to rotate so that the second cover 59 can seal and cover the consumable box 200 or open the consumable box 200, so that the consumable box 200 can be taken and placed.

[0206] In a preferred embodiment, the second cover plate 59 adopts a shape matching the consumable box 200. At the same time, the cooling shaft 57 and the first cover plate 56 are fixedly connected by means of screws and holes. The cover plate elastic member 58 used between the first cover plate 56 and the second cover plate 59 can, on the one hand, avoid hard contact between the second cover plate 59 and the consumable box 200, thereby avoiding damage to the second cover plate 59 and the consumable box 200; on the other hand, it can make up for processing and installation errors. When the flatness of the second cover plate 59 and the consumable box 200 is poor, the second cover plate 59 can be covered on the consumable box 200 at all places by means of different compression amounts of the cover plate elastic member 58 at different positions. Optionally, the cover plate elastic member 58 is a telescopic pin.

[0207] The cover plate driving unit 518 can be a motor, such as a servo motor or a stepper motor, so that the rotation distance of the second cover plate 59 is more accurate. The motor is fixed on the cover plate support frame 55, and the output shaft of the motor extends to the outer side of the cover plate support frame 55 and is connected to the cover plate driving pulley 516. The cover plate driving pulley 516 is connected to the cover plate driven pulley 517 at one end of the cooling shaft 57 through the cover plate synchronous belt 513, driving the first cover plate 56 and the second cover plate 59 to rotate at least 90° around the cooling shaft 57, preferably between 90°-135°.

[0208] When the consumable box 200 carrying the consumable box 200 is disposed behind the cooling water drain 53 , the cooling process is accompanied by the condensation water blocking module 54 isolating the air of the consumable box 200 to prevent the formation of condensation water.

[0209] The working process of the cooling device 5 is as follows:

[0210] First, the consumables box 200 is placed on the cooling device 5;

[0211] The cover driving unit 518 drives the first cover 56 to rotate, so that the second cover 59 is sealed and covers the consumable box 200, and in order to prevent the second cover 59 from exerting too much force on the consumable box 200, a telescopic pin is used between the first cover 56 and the second cover 59 as a force release device;

[0212] When condensed water appears outside the consumable box 200, the condensed water will flow out through the condensed water hole 514. At the same time, due to the sealed connection between the consumable box 200 and the second cover plate 59, the condensed water in the consumable box 200 is prevented from condensing, and the condensed water in the air is prevented from condensing and flowing into the consumable box 200.

[0213] Finally, the driving unit drives the first cover plate 56 to open the second cover plate 59 to move the consumable box 200 to another position.

[0214] The specific structure of the heating device 1 is described in detail below.

[0215] like Figure 10-13 As shown, the heating device 1 comprises:

[0216] A heating base 11, wherein the heating base 11 is provided with a sink;

[0217] The heating plate 12 is arranged in the sink of the heating base 11;

[0218] The heating jig 13 is arranged on the heating base 11, and a heating groove 132 is provided on the upper surface of the heating jig 13. The consumable box 200 is placed on the heating jig 13, and the containing unit of the consumable box 200 can be inserted into the heating groove 132 and contact with the groove wall of the heating groove 132. A breathable structure 133 is provided on the groove wall of the heating groove 132.

[0219] The overall shape of the consumable box 200 is rectangular, including multiple containing monomers. For example, in a PCR plate with multiple wells, the reagent tubes are arranged in an × pattern. The shape of the heating base 11 can also be rectangular, similar to the shape of the consumable box 200, and the area can be slightly larger to support the consumable box 200. The heating base 11 is provided with a sink to accommodate the heating plate 12. Fig.12 In the embodiment, the heating plate 12 is also generally rectangular in shape, and a temperature sensor can be integrated in the heating plate 12 to detect the temperature of the heating plate 12 and facilitate the control of the heating temperature of the reagent. The heating fixture 13 is arranged on the heating base 11, and the containing unit of the consumable box 200 can be inserted into the heating groove 132 of the heating fixture 13, thereby increasing the contact area with the consumable box 200 and improving the efficiency of heat conduction. In addition, a breathable structure 133 is provided on the groove wall of the heating groove 132. Because the upper surface of the heating fixture 13 is in contact with the containing unit and the groove wall of the heating groove 132, the breathable structure 133 is provided to facilitate the discharge of the air between the two, and the consumable box 200 can be smoothly placed on the heating fixture 13; and when the consumable box 200 is removed, the breathable structure 133 can also allow air to enter between the consumable box 200 and the heating fixture 13, so that the consumable box 200 will not be difficult to remove from the heating fixture 13, thereby improving the working efficiency of the gene library construction equipment.

[0220] like Fig.10 and Fig.11As shown, optionally, the heating fixture 13 includes a plurality of vertical bodies 131, at least two vertical bodies 131 enclose a heating groove 132, and there is a gap between two adjacent vertical bodies 131 without a receiving unit to form a breathable structure 133. Optionally, in this embodiment, four vertical bodies 131 enclose a heating groove 132, and the space enclosed by the four vertical bodies 131 is the heating groove 132. One side of the heating groove 132 enclosed by the four vertical bodies 131 can be a heating arc surface 1311 to better fit with the reagent receiving unit. Along the arrangement direction of the four vertical bodies 131, there is a gap between two adjacent vertical bodies 131 to form a breathable structure 133.

[0221] Optionally, the gap between adjacent vertical bodies 131 is 20% to 25% of the diameter of the monomer contained in the consumable box 200 .

[0222] Taking the hole PCR plate as an example, the outer diameter of a single reagent tube is 9 mm, and the gap can be preferably set to 2 mm.

[0223] Optionally, the heating fixture 13 is made of metal material, wherein the material of the heating fixture 13 can be selected from aluminum alloy.

[0224] Optionally, the heating device 1 further includes a heat insulating sheet 14 , which is disposed under the heating sheet 12 and is in contact with the lower surface of the heating sheet 12 .

[0225] The shape of the heat insulating sheet 14 can match the shape of the inner wall of the sink, and it is arranged under the heating sheet 12 to play a heat insulating role, and a plastic material can be selected.

[0226] Optionally, the heat insulation sheet 14 is provided with a heat insulation positioning hole 141, and the sink is provided with a heat insulation positioning column 111 that matches the heat insulation positioning hole 141. The heat insulation positioning hole 141 can be provided at each of the four corners of the heat insulation sheet 14.

[0227] Fig.10 Optionally, the side walls and the bottom plate of the heating base 11 are provided with gaps.

[0228] in, Fig.13 The notches shown in the figure facilitate the placement and removal of the heating sheet 12 and the heat insulating sheet 14.

[0229] Optionally, the heating device 1 further includes a heating bracket 16 , which is disposed below the heating base 11 and fixedly connected to the heating base 11 , and the heating base 11 is connected to the first shell 100 via the heating bracket 16 .

[0230] Optionally, the heating device 1 further comprises a heating fixing seat 17, which is disposed below the heating bracket 16 and fixedly connected to the heating bracket 16, and the heating fixing seat 17 comprises a heating fixing pin extending downward, which can be fixed in the first housing 100 through the heating fixing pin.

[0231] Optionally, the gene sequencing library preparation instrument may further include an anti-lifting device 2, and the specific structure of the anti-lifting device 2 is described in detail below.

[0232] Some consumable boxes 200 are provided with a sealing film to seal the solution in the consumable box 200 to prevent the solution from evaporating, leaking, being contaminated by condensed water in the air, or other types of cross-contamination, such as preventing the solution in the consumable box 200 on the heating device 1 from evaporating, preventing condensed water in the air from entering the solution in the consumable box 200 of the cooling device 5, or preventing the solution in the constant temperature plate position module (such as the first constant temperature plate position module 91) from being contaminated.

[0233] In this way, when taking the solution, it is necessary to use a pipette to puncture the sealing film and then absorb the solution; after absorbing the solution, the pipette may bring up the consumable box 200 when withdrawing from the sealing film, breaking the consumable box 200, or even spilling or cross-contaminating the solution, which will also affect the pipetting process of the pipette, and further affect the efficiency and accuracy of library construction.

[0234] Therefore, if Figure 14-Figure 20 As shown, in this embodiment, the cooling device 5, the heating device 1 and / or the normal temperature plate position module are connected to the anti-lifting device 2, and the anti-lifting device 2 is used to fix the consumable box 200 located on the cooling device 5, the heating device 1 or the normal temperature plate position module.

[0235] Specifically, the cooling device 5, the heating device 1 and the constant temperature plate module (such as the first constant temperature plate module 91, for the sake of simplicity, the cooling device 5, the heating device 1 and the constant temperature plate module (such as the first constant temperature plate module 91) are all referred to as the solution storage module below) are connected with the anti-lifting device 2. Of course, the anti-lifting device 2 can also be connected to one or two of the cooling device 5, the heating device 1 and the constant temperature plate module as needed. When the consumable box 200 is placed on the solution storage module, the anti-lifting device 2 clamps the consumable box 200, and when the consumable box 200 needs to be removed, the anti-lifting device 2 releases the consumable box 200. The sealing film is punctured with a pipette gun and then the solution is absorbed. After absorbing the solution, when the pipette gun withdraws from the sealing film, the consumable box 200 can be prevented from being lifted by the pipette gun because the anti-lifting device 2 clamps the consumable box 200, thereby preventing the consumable box 200 from being damaged, ensuring the normal transfer of the solution, and thus improving the efficiency and accuracy of library construction.

[0236] Among them, the anti-lifting device 2 includes a clamping and fixing component 21, a clamping component 22, a clamping and lifting component 23 and a clamping and linking component 24. The clamping and fixing component 21 is fixedly arranged relative to the storage seat in the solution storage module (for the sake of simplicity of description, the normal temperature base 912, the heating fixed seat 17 and the cooling base 52 are all referred to as storage seats). The clamping and lifting component 23 includes a clamping and lifting seat 231, and the clamping and lifting seat 231 can be lifted relative to the clamping and fixing component 21. The clamping and linking component 24 includes a clamping abutment 241, a clamping linking component 242 and a clamping elastic component 243. The clamping abutment 241 abuts on the clamping and lifting seat 231, and the clamping abutment 241 is connected to the clamping and lifting seat 231 through the clamping elastic component 243. One end of the clamping linking component 242 is rotatably connected to the clamping abutment 241. The clamping component 22 includes a clamping claw 221, which is movable along the second direction Y (such as Fig.15 and the left and right directions shown), the consumable box 200 is symmetrically provided with clamping claws 221 on the opposite sides, the clamping claws 221 are arranged one by one corresponding to the clamping linkage assembly 24, and the other end of the clamping linkage 242 is coaxially fixedly connected with the clamping claws 221, and each clamping claw 221 can be rotatably arranged on the storage seat to be able to clamp the consumable box 200 so that the consumable box 200 is pressed tightly against the storage seat. The clamping lifting seat 231 rises, and because the clamping abutment 241 abuts against the clamping lifting seat 231, the clamping lifting seat 231 drives the clamping abutment 241 to move upward, and the clamping abutment 241 causes the clamping linkage 242 to rotate around the fixed axis (hereinafter referred to as the clamping shaft 222), thereby driving the clamping jaws 221 to rotate. Since the clamping jaws 221 are symmetrically arranged on the opposite sides of the consumable box 200, the rotation directions of the two clamping jaws 221 are opposite, and the two clamping jaws 221 are in an open state (such as Fig.15 As shown in FIG. 2 , in order to take and place the consumable box 200, during the fixed axis rotation of the clamping linking member 242, the clamping abutment member 241 will be driven to roll rightward along the second direction Y on the clamping lifting seat 231 (as shown in FIG. 2 ). Fig.15 The clamping abutment 241 drives the clamping elastic member 243 to extend; the clamping lifting seat 231 descends, and the elastic deformation of the clamping elastic member 243 forces the clamping abutment 241 to follow the clamping lifting seat 231 to descend, and the clamping abutment 241 rolls leftward along the second direction Y on the clamping lifting seat 231 (as shown in the right side of the figure). Fig.16 The left side shown in the figure) causes the clamping link 242 to rotate in the opposite direction around the fixed axis (hereinafter referred to as the clamping shaft 222), and the clamping link 242 drives the clamping jaws 221 to rotate in the opposite direction around the fixed axis (hereinafter referred to as the clamping shaft 222), so that the two clamping jaws 221 are in a clamping state, that is, the two clamping jaws 221 clamp the consumable box 200 (such as Fig.16 As shown), the consumable box 200 can be prevented from being lifted up by the pipette, thereby preventing damage to the consumable box 200, ensuring normal solution pipetting, and thus improving the efficiency of library construction.

[0237] Furthermore, the consumable box 200 includes a box body 201 and a consumable flange 202, the consumable flange 202 is protruded on the box body 201, the solution is stored on the box body 201, and the clamping jaws 221 are clamped on the consumable flange 202 or the box body 201 to prevent the consumable box 200 from being lifted by the pipette.

[0238] When at least two of the cooling device 5, the first constant temperature plate position module 91 and the heating device 1 are connected with the anti-lifting device 2, each of the above devices corresponds to at least one consumable box 200, and each consumable box 200 corresponds to at least one group of clamping jaws 221 modules, and each group of clamping jaws 221 modules includes two clamping jaws 221 arranged oppositely along the second direction Y, so that each consumable box 200 can be clamped to play the role of preventing lifting. As for whether each group of clamping jaws 221 modules is driven by the same clamping lifting seat 231, it can be set according to actual conditions.

[0239] In this embodiment, the cooling device 5, the first constant temperature plate position module 91 and the heating device 1 are all connected to the anti-lifting device 2, each of the above devices corresponds to a consumable box 200, each consumable box 200 corresponds to two groups of clamping jaws 221 modules, and the two groups of clamping jaws 221 modules are arranged at intervals along the first direction X, so as to clamp the four corners of the consumable box 200, thereby increasing stability. That is to say, in this embodiment, the number of the clamping jaw 221 modules is six groups, and each group of the clamping jaw 221 modules includes two clamping jaws 221 arranged opposite to each other along the second direction Y.

[0240] Along the first direction X, two clamping jaws 221 arranged opposite to each other in the two groups of clamping jaw 221 modules are coaxially connected.

[0241] In this embodiment, the six groups of clamping jaws 221 modules are driven by two clamping lifting seats 231, and the six groups of clamping jaws 221 modules can simultaneously open or clamp the corresponding consumable boxes 200. Of course, the number of clamping lifting seats 231 is not limited to the above two, and can be set after comprehensive consideration of the number and distribution positions of the actual clamping jaws 221 modules.

[0242] like Fig.15 and Fig.16 As shown, since different specifications of consumable boxes 200 may be placed on the cooling device 5, the first constant temperature plate module 91 and the heating device 1, the shape and size of each clamping jaw 221 in the six groups of clamping jaws 221 modules may be different. This embodiment does not limit this and can be set according to actual conditions.

[0243] like Fig.17As shown, the setting of the clamping elastic member 243 can enable the clamping abutment 241 to adapt to the fixed-axis rotation of the clamping linkage 242, so that the clamping abutment 241 and the clamping linkage 242 will not interfere with each other in movement, and the elastic deformation of the clamping elastic member 243 will also drive the clamping abutment 241 to reset when the clamping lifting seat 231 descends, so as to realize the clamping of the consumable box 200 by the clamping claw 221.

[0244] Furthermore, the clamping assembly 22 also includes a clamping shaft 222, which can be rotatably arranged on the storage seat. The clamping jaw 221 and the clamping linkage 242 are both installed on the clamping shaft 222. The other end of the clamping linkage 242 rotates, which can drive the clamping shaft 222 to rotate, and then the clamping shaft 222 drives the clamping jaw 221 to rotate.

[0245] In this embodiment, the number of the clamping shafts 222 is six, and each clamping shaft 222 is mounted with two clamping jaws 221 that are oppositely arranged along the first direction X and a clamping linkage 242 .

[0246] like Fig.14 and Fig.18 As shown, the clamping jaw 221 includes an integrally formed clamping rotating portion 2211, a clamping portion 2212 and a clamping connecting portion 2213. The clamping rotating portion 2211 is connected to the clamping portion 2212 via the clamping connecting portion 2213. The clamping rotating portion 2211 is rotatably arranged on the storage seat. Specifically, the clamping rotating portion 2211 is fixedly connected to the clamping rotating shaft 222 and rotates around the central axis of the clamping rotating shaft 222. The clamping portion 2212 and the clamping connecting portion 2213 form a clamping area, and the consumable flange 202 of the consumable box 200 is clamped in the clamping area. The shape of the clamping jaw 221 is more convenient for clamping the consumable flange 202.

[0247] like Fig.14 , Fig.19 and Fig. 20 As shown, the anti-belt lifting device 2 further includes a clamping detection component 25 , and the clamping drive seat 233 is guided by the clamping detection component 25 .

[0248] Furthermore, the clamping detection assembly 25 includes a clamping guide block 251 and a clamping guide rail 252. The clamping guide rail 252 is fixedly mounted on the clamping fixing assembly 21. The clamping guide rail 252 is extended in the vertical direction. The clamping guide block 251 is mounted on the clamping drive seat 233. The clamping guide block 251 can be slidably disposed on the clamping guide rail 252.

[0249] Specifically, the clamping detection assembly 25 and the clamping lifting seat 231 are arranged in a one-to-one correspondence.

[0250] like Fig.14 , Fig.15, Fig.16 , Fig.19 and Fig. 20 As shown, the clamping and lifting assembly 23 further includes a clamping driving seat 233 , on which the clamping and lifting seat 231 is fixedly mounted, and the clamping driving seat 233 can be lifted and lowered relative to the clamping and fixing assembly 21 .

[0251] Furthermore, the clamping and lifting assembly 23 also includes a clamping driving source 232, which can drive the clamping and driving seat 233 to rise and fall. Specifically, each clamping and lifting seat 231 is installed on the same clamping and driving seat 233. In other words, each clamping and lifting seat 231 is driven by the same clamping and driving source 232, and each clamping and lifting seat 231 rises and falls synchronously.

[0252] In this embodiment, each clamping lifting seat 231 is driven by the same clamping driving source 232. Of course, a clamping driving source 232 may be separately provided for each clamping lifting seat 231. Therefore, there is no limitation to this, and it may be set according to actual conditions.

[0253] Furthermore, the clamping drive source 232 may be a through-type stepping motor, including a clamping output shaft 2321 and a motor body 2322. The clamping output shaft 2321 is fixedly mounted on the clamping fixed assembly 21. The clamping output shaft 2321 can be lifted relative to the motor body 2322. The motor body 2322 is connected to the clamping drive seat 233. The clamping output shaft 2321 is stationary, and the motor body 2322 is lifted and lowered, driving the clamping drive seat 233 to lift and lower, thereby driving each clamping lifting seat 231 to lift and lower.

[0254] Further, the anti-lifting device 2 also includes a clamping detection component 26, which includes a clamping sensor 261 and a clamping baffle 262. The clamping baffle 262 is installed on the clamping drive seat 233, and the clamping sensor 261 is installed on the clamping fixing component 21. The clamping sensor 261 can detect whether the clamping baffle 262 is in place. When the clamping baffle 262 is in place, the clamping jaw 221 clamps the consumable box 200 with an appropriate force. The setting of the clamping detection component 26 can prevent the clamping jaw 221 from damaging the consumable box 200 due to excessive force, thereby extending the service life of the consumable box 200.

[0255] In this embodiment, since each clamping lifting seat 231 is lifted and lowered synchronously, a clamping detection assembly 26 is arranged near one of the clamping lifting seats 231 to ensure that the clamping force of each clamping jaw 221 is appropriate. Of course, the clamping detection assembly 26 can also be arranged near both clamping lifting seats 231, which is not limited in this embodiment.

[0256] Furthermore, the clamping lifting seat 231 includes a clamping abutment portion 2311 and a clamping mounting portion 2312, the clamping abutment portion 2311 and the clamping mounting portion 2312 are arranged vertically, the clamping abutment member 241 abuts against the clamping abutment portion 2311, the clamping mounting portion 2312 is installed on the clamping driving seat 233, and the size of the clamping abutment portion 2311 along the second direction Y is greater than the size of the clamping mounting portion 2312 along the second direction Y. Such an arrangement ensures that each clamping abutment member 241 can abut against the clamping abutment portion 2311 while reducing the volume of the clamping driving seat 233, and ensures that each clamping abutment member 241 has space to roll along the second direction Y.

[0257] Further, the clamping and fixing assembly 21 includes a clamping and fixing base 211 and a clamping and fixing stand 212, the clamping and fixing base 211 and the clamping and fixing stand 212 are fixedly connected to form an L-shape, the clamping and fixing base 211 and the clamping and fixing stand 212 are both fixedly arranged relative to the bracket, one end of the clamping output shaft 2321 of the clamping driving source 232 is fixed to the clamping and fixing base 211, and the other end is fixedly connected to the clamping and fixing stand 212. The L-shaped clamping and fixing base 211 and the clamping and fixing stand 212 facilitate the installation of the clamping driving source 232, the clamping driving seat 233, the clamping lifting seat 231 and the clamping detection assembly 25, so as to guide the clamping driving seat 233 and the clamping lifting seat 231.

[0258] Furthermore, the clamping lifting assembly 23 also includes a clamping mounting seat 234 , the other end of the clamping output shaft 2321 is fixed on the clamping mounting seat 234 , and the clamping mounting seat 234 is fixedly mounted on the clamping fixed stand 212 .

[0259] Specifically, the clamping guide rail 252 and the clamping sensor 261 are both fixedly mounted on the clamping fixed stand 212 .

[0260] like Figure 21a-Figure 27b , the specific structure of the oscillation device 8 is described in detail below.

[0261] like Fig.21a , Figure 21b , Fig. 22As shown, in the present embodiment, an oscillation device 8 is provided, comprising an oscillation working support 81, an oscillation motor assembly 82, an oscillation eccentric wheel assembly 83, an oscillation guide mechanism 84, an oscillation tray 85 and a reset assembly 86 connected to the first housing 100, wherein the oscillation motor assembly 82 comprises an oscillation stepper motor 823 and a coupling assembly 824, and the oscillation stepper motor 823 is installed on the oscillation working support 81. The output end of the oscillation stepper motor 823 is eccentrically arranged through the oscillation working support 81 and the oscillation eccentric wheel assembly 83, and both are connected to the coupling assembly 824. That is, the coupling assembly 824 is provided with two groups of mounting holes, and the two groups of mounting holes are eccentrically arranged up and down, and the output end of the oscillation stepper motor 823 is connected to one group of mounting holes after passing through the oscillation working support 81, and the oscillation eccentric wheel assembly 83 is connected to the other group of mounting holes. The oscillation stepper motor 823 drives the eccentric wheel of the oscillation eccentric wheel assembly 83 to move in a plane through the coupling assembly 824.

[0262] The oscillating tray 85 is connected to the oscillating eccentric wheel assembly 83 , and the oscillating eccentric wheel assembly 83 can rotate relative to the oscillating tray 85 . A detachable consumable box 200 is placed on the oscillating tray 85 .

[0263] The oscillation guide mechanism 84 is arranged between the oscillation working support 81 and the oscillation tray 85. The oscillation guide mechanism 84 is located on one side of the oscillation eccentric wheel assembly 83. The oscillation guide mechanism 84 limits the oscillation tray 85 to move along the first direction X and the second direction Y in the horizontal plane.

[0264] The reset assembly 86 includes a first fixing member 863 , an oscillation reset spring 864 , and a crank 8653 and a connecting rod member 865 . The first fixing member 863 is arranged on the oscillation working bracket 81 or at the bottom of the oscillation tray 85 .

[0265] The crank 8653 connecting rod 865 is installed on the coupling assembly 824 and can rotate relative to the coupling assembly 824. The rotation center of the crank 8653 connecting rod 865 is not coaxial with the oscillation stepping motor 823 and / or the coupling assembly 824. When the coupling assembly 824 rotates, the crank 8653 connecting rod 865 can rotate relative to the coupling assembly 824. Due to the eccentric movement of the oscillation eccentric wheel assembly 83, the oscillation reset spring 864 is stretched and deformed, preventing the oscillation reset spring 864 from being wrapped around the coupling assembly 824.

[0266] One end of the oscillation return spring 864 is connected to the crank 8653 connecting rod 865, and the other end is connected to the first fixing member 863. When the oscillation stepping motor 823 works, the oscillation return spring 864 is stretched, but the oscillation return spring 864 will not be wound around the coupling assembly 824.

[0267] Fig. 22In this embodiment, the oscillating stepper motor 823 rotates to drive the oscillating eccentric wheel assembly 83 to move through the coupling assembly 824, thereby driving the oscillating tray 85 connected to the oscillating eccentric wheel assembly 83 to move in the horizontal plane along the first direction X and the second direction Y to shake the test tube so that the liquid in each test tube can be vortex mixed around the central axis of the test tube.

[0268] The oscillation guide mechanism 84 provides precise guidance for the movement of the oscillation tray 85 in the horizontal plane, and limits the movement amplitude of the oscillation tray 85 in the first direction X and the second direction Y in the horizontal plane, preventing the oscillation tray 85 from shaking with excessive displacement during the oscillation process, thereby preventing the solution in the consumable box 200 from being shaken out.

[0269] In addition, the consumable box 200 can be quickly disassembled and placed on the shaking tray 85, and the consumable box 200 is removed synchronously after the shaking is completed.

[0270] At the same time, when the oscillating stepper motor 823 drives the oscillating tray 85 to move in the horizontal plane, the oscillating reset spring 864 is stretched, and when the oscillating stepper motor 823 stops working, the oscillating reset spring 864 contracts, pulling the oscillating stepper motor 823 to reset. This allows the consumable box 200 on the oscillating tray 85 to return to the origin, making it easier for the automation system to identify the position of the consumable box 200, so that the oscillating device 8 can be applied to the automation system.

[0271] In the prior art, the reaction samples are often mixed by a pipette. In this embodiment, by providing an oscillating device 8, the reaction samples are mixed by vibration, which greatly improves the mixing efficiency and the detection throughput.

[0272] In this embodiment, the consumable box 200 is detachably placed on the vibration tray 85. The specific manner of "detachable" includes that when the consumable box 200 is placed on the vibration tray 85 through the clamping mechanism 62, the consumable box 200 can be clamped by the vibration tray 85. When the clamping mechanism 62 clamps, it can quickly detach from the vibration tray 85. When the consumable box 200 detaches from the vibration tray 85, the clamping mechanism 62 uses less force, and the solution in the consumable box 200 will not be shaken and splashed due to the detachment from the vibration tray 85.

[0273] In this embodiment, the specific structure of the above-mentioned consumable box 200 being detachable on the oscillating tray 85 may include: a plunger spring 853 is provided on the upper side wall of the oscillating tray 85, and the side wall of the consumable box 200 is clamped by the plunger spring 853. Or, a first magnetic attraction member is provided on the oscillating tray 85, and a second magnetic attraction member is provided on the consumable box 200, the first magnetic attraction member and the second magnetic attraction member are mutually attracted, and the first magnetic attraction member is powered on and off to control the first magnetic attraction member and the second magnetic attraction member, so as to realize the rapid disassembly of the consumable box 200 on the oscillating tray 85.

[0274] The oscillation frequency range of the oscillating stepper motor 823 in this embodiment is 500rpm-2000rpm. Specifically, the oscillation frequency of the oscillating stepper motor 823 is 500rpm, 1000rpm, 1500rpm, and 2000rpm. If the oscillation frequency of the oscillating stepper motor 823 is lower than 500rpm, the oscillation effect is poor. If the oscillation frequency of the oscillating stepper motor 823 is higher than 2000rpm, the oscillation is violent, which may easily cause the solution in the test kit to spill out.

[0275] like Fig. 22 As shown, the oscillation working bracket 81 includes an oscillation support frame 811 and an oscillation mounting plate 812. The oscillation mounting plate 812 is installed above the oscillation support frame 811. The oscillation motor assembly 82 is arranged in the oscillation support frame 811 and connected to the oscillation mounting plate 812. The oscillation motor assembly 82 is located below the oscillation mounting plate 812. The output end of the oscillation motor assembly 82 passes through the oscillation mounting plate 812 and is connected to the oscillation eccentric wheel assembly 83. The oscillation guide mechanism 84 is connected to the oscillation mounting plate 812 and is located on the upper surface of the oscillation mounting plate 812.

[0276] By setting an oscillation support frame 811 and an oscillation mounting plate 812, the oscillation support frame 811 is a hollow structure, and the oscillation stepper motor 823 is set in the space of the oscillation support frame 811, and its output end extends out of the oscillation mounting plate 812. The structure is compact and integral, and the oscillation support frame 811 is set as a hollow structure. The overall structure is light in weight and easy to install.

[0277] Preferably, an eccentric wheel mounting groove is provided at the bottom of the vibration tray 85, and the gap between the vibration eccentric wheel assembly 83 and the eccentric wheel mounting groove ranges from 0.1 mm to 0.2 mm. The clearance between the eccentric wheel mounting groove and the vibration eccentric wheel assembly 83 ensures that the vibration eccentric wheel assembly 83 rotates, but does not drive the vibration tray 85 to rotate. During the eccentric rotation of the vibration eccentric wheel assembly 83, it can only push the vibration tray 85 to move along the first direction X and the second direction Y in the horizontal plane to generate vibration, and the vibration tray 85 and the vibration eccentric wheel assembly 83 will not be disengaged. Preferably, the gap between the vibration eccentric wheel assembly 83 and the eccentric wheel mounting groove is 0.1 mm.

[0278] Preferably, a motor mounting hole is provided on the side of the oscillation mounting plate 812 facing the eccentric wheel, and the output end of the oscillation motor assembly 82 extends out of the motor mounting hole and is connected to the oscillation eccentric wheel assembly 83. A weight-reducing groove 8122 is provided in the circumference of the motor mounting hole, and the oscillation reset spring 864 and the first fixing member 863 are provided in the weight-reducing groove 8122. Further preferably, the weight-reducing grooves 8122 are evenly distributed along the circumference of the motor mounting hole, and the weight-reducing grooves 8122 are U-shaped. There are four groups of weight-reducing grooves 8122 in this embodiment.

[0279] The oscillation device 8 further includes a tray support plate 854 disposed between the oscillation tray 85 and the oscillation mounting plate 812, so as to support the oscillation tray 85. Due to the structural dimensions of the coupling assembly 824 in the oscillation device 8 in the vertical direction, the tray support plate 854 is provided to ensure the structural dimensions of the coupling assembly 824, and also to facilitate the connection between the oscillation tray 85 and the oscillation guide mechanism 84 and the oscillation eccentric wheel assembly 83. Specifically, the tray support plate 854 is a square ring member.

[0280] Preferably, if Fig. 22 , Fig.23 , Fig.24a , Figure 24b As shown, the coupling assembly 824 includes: a first coupling member 8241 and a second coupling member 8242 are arranged in parallel up and down, and an oscillating connecting shaft 8243, one end of the oscillating connecting shaft 8243 is connected to the first coupling member 8241, and the other end is connected to the second coupling member 8242. Preferably, the first coupling member 8241 and the oscillating connecting shaft 8243 are interference fitted, and the second coupling member 8242 and the oscillating connecting shaft 8243 are interference fitted. In other embodiments, the first coupling member 8241, the second coupling member 8242, and the oscillating connecting shaft 8243 are an integrally processed structure.

[0281] The crank 8653 connecting rod 865 is arranged between the first coupling 8241 and the second coupling 8242, and is sleeved on the oscillating connecting shaft 8243. The first coupling 8241 is connected to the output shaft of the oscillating stepper motor 823, and the second coupling 8242 is connected to the oscillating eccentric wheel assembly 83. The first coupling 8241 and the second coupling 8242 rotate synchronously, driving the oscillating tray 85 to move along the first direction X and the second direction Y in the horizontal plane, thereby generating oscillations and mixing the solution evenly. At the same time, the structure of the first coupling 8241, the second coupling 8242 and the oscillating connecting shaft 8243 is arranged in an "I" shape, with a simple structure, convenient processing and installation, and low cost.

[0282] Preferably, the crank 8653 connecting rod 865 includes a sleeve and a crank 8653 connected to the sleeve, the sleeve is sleeved on the oscillation connection shaft 8243 and can rotate relative to the oscillation connection shaft 8243, and one end of the oscillation reset spring 864 is connected to the crank 8653. The crank 8653 fixes the oscillation reset spring 864, and the sleeve rotates relative to the oscillation connection shaft 8243, thereby preventing the oscillation reset spring 864 from being wound around the sleeve. When the first coupling 8241 and the second coupling 8242 rotate synchronously, the oscillation reset spring 864 is only stretched and deformed.

[0283] Preferably, the shaft sleeve includes a first connecting member 8651 and a second connecting member 8652, which are coaxial and relatively arranged on the outer periphery of the oscillating connecting shaft 8243, and the first connecting member 8651 and the second connecting member 8652 are both provided with semicircular holes, and the two are relatively connected and sleeved on the oscillating connecting shaft 8243. The crank 8653 is connected to the second connecting member 8652. Preferably, the second connecting member 8652 and the crank 8653 are integrally formed. The two are integrally formed, which is convenient for installation and processing and saves installation space.

[0284] Preferably, a second fixing member 866 is provided on the crank 8653, one end of the oscillation reset spring 864 is connected to the second fixing member 866, and the other end is connected to the first fixing member 863. In other embodiments, a connecting hole is provided on the crank 8653, one end of the oscillation reset spring 864 is connected to the connecting hole, and the other end is connected to the first fixing member 863.

[0285] One end of the oscillation connecting shaft 8243 is interference fitted with the first coupling member 8241, and the other end is interference fitted with the second oscillation connecting shaft 8243. To facilitate the assembly of the crank 8653 and the connecting rod member 865, the crank 8653 and the connecting rod member 865 are divided into two parts, a first connecting member 8651 and a second connecting member 8652. Connecting holes are provided on the first connecting member 8651 and the second connecting member 8652, and screws are inserted through the connecting holes to connect the first connecting member 8651 and the second connecting member 8652.

[0286] Preferably, the distance between the center of the oscillating eccentric wheel assembly 83 and the center of the oscillating stepper motor 823 ranges from 1mm to 1.5mm. Specifically, the distance between the center of the oscillating eccentric wheel assembly 83 and the center of the oscillating stepper motor 823 (i.e., the center of the second coupling 8242) ranges from 1mm to 1.5mm. By the eccentric rotation of the oscillating eccentric wheel assembly 83, the oscillating tray 85 is displaced in the horizontal plane along the first direction X and the second direction Y, thereby producing an oscillating effect. Preferably, the distance between the center of the oscillating eccentric wheel assembly 83 and the center of the oscillating motor assembly 82 ranges from 1mm or 1.5mm. When the distance between the above two is less than 1mm, the displacement produced by the eccentric motion of the oscillating eccentric wheel assembly 83 is small, resulting in insufficient oscillation effect. When the distance between the above two is greater than 1.5mm, the displacement produced by the eccentric motion of the oscillating eccentric wheel assembly 83 is large, which easily causes the solution to shake violently and affects the mixing effect.

[0287] Preferably, the oscillating eccentric wheel assembly 83 includes an oscillating cam follower 831, and the shaft of the oscillating cam follower 831 is connected to the coupling. The bearing end of the oscillating cam follower 831 is connected to the bottom of the oscillating tray 85. The distance between the center of the oscillating cam follower 831 and the center of the oscillating stepping motor 823 ranges from 1mm to 1.5mm. When the distance between the above two is less than 1mm, the displacement generated by the eccentric motion of the oscillating cam follower 831 is small, resulting in insufficient oscillation effect. When the distance between the above two is greater than 1.5mm, the displacement generated by the eccentric motion of the oscillating cam follower 831 is large, which easily causes the solution to shake violently, affecting the mixing effect. The oscillating cam follower 831 is selected according to actual needs and is a conventional structure on the market.

[0288] In other embodiments, the oscillating eccentric wheel assembly 83 includes an eccentric wheel, an eccentric oscillation connecting shaft 8243 and an intermediate oscillation connecting shaft 8243 bearing, one end of the eccentric oscillation connecting shaft 8243 is connected to a coupling, and the other end of the eccentric oscillation connecting shaft 8243 is connected to the inner ring of the intermediate oscillation connecting shaft 8243 bearing. The eccentric wheel is sleeved on the outer ring of the intermediate oscillation connecting shaft 8243 bearing, and at the same time, the eccentric wheel is connected to the oscillation tray 85 and can rotate relative to the oscillation tray 85. Specifically, an eccentric wheel mounting groove is provided at the bottom of the above-mentioned oscillation tray 85, and the eccentric wheel and the eccentric wheel mounting groove are clearance-matched. Preferably, the gap range between the eccentric wheel and the eccentric wheel mounting groove is 0.1mm-0.2mm. Further preferably, the gap between the eccentric wheel and the eccentric wheel mounting groove is 0.1mm, 0.15mm and 0.2mm.

[0289] Preferably, the distance between the center of the eccentric wheel and the center of the oscillating stepper motor 823 (i.e., the center of the second coupling 8242) is in the range of 1 mm to 1.5 mm. Preferably, the distance between the center of the eccentric wheel and the center of the oscillating stepper motor 823 (i.e., the center of the second coupling 8242) is in the range of 1 mm or 1.5 mm.

[0290] Preferably, in this embodiment, the oscillating eccentric wheel assembly 83 is an oscillating cam follower 831, and two groups of oscillating guide mechanisms 84 are provided, and the two groups of oscillating guide mechanisms 84 are symmetrically arranged on both sides of the oscillating motor assembly 82, and specifically, the two groups of oscillating guide mechanisms 84 are symmetrically arranged on both sides of the oscillating stepping motor 823. When the oscillating tray 85 moves in the horizontal plane, the oscillating guide mechanism 84 guides the movement of the oscillating tray 85 along the first direction X and the second direction Y, and the two groups of oscillating guide mechanisms 84 can ensure that the oscillating tray 85 is uniformly stressed, thereby ensuring that the oscillating tray 85 moves smoothly in the horizontal plane and shakes evenly.

[0291] In this embodiment, an eccentric wheel mounting groove is provided at the bottom of the oscillation tray 85, and the oscillation cam follower 831 is clearance-matched with the eccentric wheel mounting groove. Preferably, the clearance between the oscillation cam follower 831 and the eccentric wheel mounting groove is in the range of 0.1 mm to 0.2 mm. Further preferably, the clearance between the oscillation cam follower 831 and the eccentric wheel mounting groove is 0.1 mm, 0.15 mm and 0.2 mm.

[0292] Preferably, if Fig. 22 , Fig.25a , Fig.25b , Fig.26a and Figure 26b As shown, the oscillation guide mechanism 84 includes a first oscillation guide component, a guide connection component 845 and a second oscillation guide component. The first oscillation guide component is used to guide the oscillation tray 85 in the first direction X, and the second oscillation guide component is used to guide the oscillation tray 85 in the second direction Y. The first oscillation guide component of the oscillation tray 85 is connected to the oscillation working bracket 81. Specifically, the first oscillation guide component is connected to the oscillation mounting plate 812, and the guide connection component 845 is arranged between the first oscillation guide component and the second oscillation guide component, and the first oscillation guide component and the second oscillation guide component are both connected to the guide connection component 845. The oscillation tray 85 is connected to the second oscillation guide component. The first oscillation guide component and the second oscillation guide component are stacked and fixed, and the oscillation tray 85 is limited to a small amplitude shaking in the horizontal plane during the movement, and the shaking amplitude radius range is 1mm-1.5mm, so that the solution oscillates smoothly during the oscillation process.

[0293] Preferably, if Fig.25a , Fig.25b As shown, the first oscillation guide assembly includes a first oscillation slide rail 841 and a first oscillation slider 842 that slide in cooperation with each other. The first oscillation slide rail 841 is arranged along the first direction X and is connected to the oscillation working bracket 81. Specifically, the first oscillation slide rail 841 is connected to the oscillation mounting plate 812, and the first oscillation slider 842 is connected to the guide connection assembly 845. More specifically, the first oscillation slider 842 and the guide connection assembly 845 are fastened and connected by four groups of bolts. Through the mutual cooperation and sliding of the first oscillation slide rail 841 and the first oscillation slider 842, the precise guidance of the movement of the oscillation tray 85 in the first direction X is achieved, and the movement amplitude is guaranteed.

[0294] Preferably, the second oscillation guide assembly includes a second oscillation rail 843 and a second oscillation slider 844 that slide in cooperation with each other, and the second oscillation rail 843 is arranged along the second direction Y and connected to the oscillation tray 85. The second oscillation slider 844 is connected to the guide connection assembly 845, and specifically, the second oscillation slider 844 is fastened to the guide connection assembly 845 by four groups of bolts. Through the cooperation and sliding of the second oscillation rail 843 and the second oscillation slider 844, the second direction Y movement of the oscillation tray 85 is accurately guided to ensure the movement amplitude.

[0295] Preferably, a guide rail mounting piece is provided at the bottom of the oscillating tray 85, on which a guide rail mounting groove is provided, and the second oscillating slide rail 843 is installed in the guide rail mounting groove. Specifically, the guide rail mounting piece is fastened to the bottom of the tray bottom plate 851 by screws.

[0296] Preferably, if Fig.25a , Fig.25b , Fig.26a , Figure 26b As shown, the guide connection assembly 845 includes a first guide connection member 8451, a second guide connection member 8452 and a fastening connection member 8453, wherein a first oscillating slider 842 mounting groove is provided on the bottom surface of the first guide connection member 8451, the first oscillating slider 842 is provided in the first oscillating slider 842 mounting groove, and the first oscillating slider 842 is fastened and connected to the first guide connection member 8451 by four groups of bolts. Specifically, a first slider fastening hole 84512 is provided at the bottom of the first oscillating slider 842 mounting groove, and a screw is inserted through the first slider fastening hole 84512 to connect the first guide connection member 8451 and the first oscillating slider 842.

[0297] The second guide connector 8452 is provided with a second oscillating slider 844 mounting groove on the top surface thereof. The second oscillating slider 844 is disposed in the second oscillating slider 844 mounting groove. The second oscillating slider 844 is fastened to the second guide connector 8452 by four sets of bolts. The second oscillating slider 844 is supported by the second guide connector 8452 to facilitate fixing the second oscillating slider 844. Specifically, a second slider fastening hole is provided at the bottom of the second oscillating slider 844 mounting groove. Screws are inserted through the second slider fastening hole to connect the second guide connector 8452 and the second oscillating slider 844.

[0298] A fastening installation groove 84513 is provided on the side of the first guide connecting member 8451 and the second guide connecting member 8452. The second guide connecting member 8452 is stacked on the top of the first guide connecting member 8451. The fastening connecting member 8453 is arranged in the fastening installation groove 84513 to connect the first guide connecting member 8451 and the second guide connecting member 8452.

[0299] The outer dimensions of the first guide connecting member 8451 and the second guide connecting member 8452 are the same as the structures arranged thereon. After the connecting member 8453 is installed and tightened, the outer dimensions of the two are not affected. The overall structure is small in size and does not occupy too much space. The overall structure of the oscillation device 8 is compact.

[0300] Regarding the specific structure of the above-mentioned vibration tray 85, as Fig.27a and Figure 27b As shown, the oscillating tray 85 includes a tray bottom plate 851, a tray stopper 852 and a plunger spring 853, wherein the tray bottom plate 851 is connected to the oscillating eccentric wheel assembly 83 and is also connected to the oscillating guide mechanism 84. Specifically, an eccentric wheel mounting groove is provided at the bottom of the tray bottom plate 851, and the oscillating cam follower 831 is installed in the eccentric wheel mounting groove.

[0301] The tray stopper 852 is disposed at the boundary of the tray bottom plate 851. Specifically, the tray stopper 852 is disposed at the four sides of the tray bottom plate 851, or the tray stopper 852 is disposed at the corners of the tray bottom plate 851, or the tray stopper 852 is disposed at the corners and sides of the tray bottom plate 851 at the same time.

[0302] The plunger spring 853 is installed on the inner side of the tray limiter 852 , and the consumable box 200 is placed on the tray bottom plate 851 . The plunger spring 853 can quickly clamp the consumable box 200 , and can quickly release the consumable box 200 when the consumable box 200 is moved away.

[0303] In this embodiment, the plunger spring 853 is used to clamp the side wall of the consumable box 200 located on the tray bottom plate 851. After the shaking is completed, the consumable box 200 is taken away by the clamping mechanism 62, thereby realizing fast taking and placing.

[0304] Preferably, the tray limiting members 852 are disposed at the four corners of the tray bottom plate 851 , and the tray limiting members 852 are in a right-angle shape.

[0305] Preferably, a buffer 88 is provided on the tray bottom plate 851 , and the consumable box 200 is placed on the buffer 88 , which can prevent slipping on the one hand, and avoid direct contact between the tray bottom plate 851 and the consumable box 200 on the other hand, thereby avoiding noise.

[0306] like Figure 28-Figure 33 As shown, the specific structure of the calibration device 7 is described in detail below.

[0307] Fig.28 FIG. 8 is a schematic diagram of a calibration device 7. Fig.28As shown, the calibration device 7 includes a second shell 71, which is roughly rectangular. The top surface of the second shell 71 is a placement surface 711. The placement area for placing the consumable box 200 is defined above the placement surface 711. A first calibration fixed block 712 is provided on the first calibration side of the placement area in the first direction X, and a first calibration movable block 72 is provided on the second calibration side of the placement area in the first direction X. The first calibration movable block 72 can move along the first direction X. When the consumable box 200 is placed in the placement area, the first calibration movable block 72 can push the consumable box 200 to the position of the first calibration fixed block 712 and abut against the first calibration fixed block 712 to realize the calibration of the consumable box 200 in the first direction X. In addition, a second calibration fixed block 713 is provided on the first calibration side of the placement area in the second direction Y, and a second calibration movable block 73 is provided on the second calibration side of the placement area in the first direction X. The second calibration movable block 73 can be movable along the second direction Y. When the consumable box 200 is placed in the placement area, the second calibration movable block 73 can push the consumable box 200 to the position of the second calibration fixed block 713 and abut against the second calibration fixed block 713 to realize the calibration of the consumable box 200 in the second direction Y.

[0308] It should be noted that, in this embodiment, the second calibration side in the first direction X is arranged opposite to the first calibration side in the first direction X, and the second calibration side in the second direction Y is arranged opposite to the first calibration side in the second direction Y.

[0309] By moving the first calibration movable block 72 and the second calibration movable block 73, the consumable box 200 can be positioned in the space defined by the first calibration fixed block 712, the second calibration fixed block 713, the first calibration movable block 72 and the second calibration movable block 73, so that the position of the consumable box 200 is calibrated so that the clamping mechanism 62 can accurately clamp the calibrated consumable box 200.

[0310] like Fig.28 As shown, in this embodiment, the first calibration fixing block 712 and the second calibration fixing block 713 are fixedly arranged on the placement surface 711, especially at the edge of the placement surface 711. The number of the first calibration fixing block 712 and the second calibration fixing block 713 can be multiple, for example Fig.28 There are two first calibration fixing blocks 712 and two second calibration fixing blocks 713. The first calibration fixing block 712 and the second calibration fixing block 713 have guiding slopes on the side facing the placement area, so that when the consumable box 200 is placed in the placement area, the guiding slopes can guide the consumable box 200 to be placed on the placement surface 711.

[0311] like Fig.28 As shown, the shapes of the first calibration movable block 72 and the second calibration movable block 73 can be cylindrical or other shapes.

[0312] like Fig.28 As shown, a first calibration groove 714 and a second calibration groove 715 are provided on the placement surface 711, the first calibration movable block 72 is inserted into the first calibration groove 714, and the second calibration movable block 73 is inserted into the second calibration groove 715. The shapes of the first calibration groove 714 and the second calibration groove 715 can be long strips, wherein the first calibration groove 714 extends along the first direction X, and the second calibration groove 715 extends along the second direction Y, so that the first calibration movable block 72 extends along the first direction X, and the second calibration movable block 73 extends along the second direction Y.

[0313] like Fig.28 As shown, in this embodiment, there are two first calibration movable blocks 72 and two second calibration movable blocks 73, and correspondingly, there are two first calibration slots 714 and two second calibration slots 715. In other embodiments, the number of first calibration movable blocks 72 and second calibration movable blocks 73 can be adjusted as needed.

[0314] In this embodiment, the two first calibration movable blocks 72 move simultaneously, and the two second calibration movable blocks 73 move simultaneously.

[0315] The first calibration movable block 72 and the second calibration movable block 73 can be driven independently or in linkage with each other by a calibration driving mechanism 76 installed inside the second housing 71 .

[0316] In this embodiment, the calibration driving mechanism 76 mentioned later drives the first calibration movable block 72 and the second calibration movable block 73 to approach or move away from the placement area at the same time, that is, the first calibration movable block 72 and the second calibration movable block 73 are linked to each other.

[0317] Fig.29 and Fig.30 Schematic diagrams of the calibration device 7 after the second housing 71 is removed are shown respectively. Fig.29 and Fig.30 As shown, the calibration device 7 also includes a first calibration mounting seat 74, two first calibration movable blocks 72 are fixedly mounted on the first calibration mounting seat 74, the first calibration mounting seat 74 is fixedly connected to the first calibration slider 741, the first calibration slider 741 is slidably connected to the first calibration guide rail 742, the first calibration guide rail 742 is fixedly mounted on the second shell 71, and the first calibration guide rail 742 extends along the first direction X, whereby, when the first calibration mounting seat 74 slides on the first calibration guide rail 742, the two first calibration movable blocks 72 move along the first direction X.

[0318] Similar, such as Fig.29 and Fig.30As shown, the calibration device 7 also includes a second calibration mounting seat 75, two second calibration movable blocks 73 are fixedly mounted on the second calibration mounting seat 75, the second calibration mounting seat 75 is fixedly connected to the second calibration slider 751, the second calibration slider 751 is slidably connected to the second calibration guide rail 752, the second calibration guide rail 752 is fixedly mounted on the second housing 71, and the second calibration guide rail 752 extends along the second direction Y, so that when the second calibration mounting seat 75 slides on the second calibration guide rail 752, the two second calibration movable blocks 73 move along the second direction Y.

[0319] like Fig.30 As shown, the first calibration mounting seat 74 is also connected to a first calibration cam follower 743, and the second calibration mounting seat 75 is also connected to a second calibration cam follower 753. The first calibration cam follower 743 and the second calibration cam follower 753 are used to connect with the calibration driving mechanism 76. The calibration driving mechanism 76 drives the first calibration cam follower 743 and the second calibration cam follower 753 to move, thereby driving the first calibration mounting seat 74 and the second calibration mounting seat 75 to move.

[0320] Fig.31 FIG. 8 is a schematic diagram of a calibration drive mechanism 76. Fig.30 and Fig.31 As shown, the calibration drive mechanism 76 includes a fixed seat 761, a third calibration drive assembly 762 and a lifting platform 763, and the fixed seat 761 is L-shaped ( Fig.31 Only the bottom of the fixing base 761 is shown in FIG. 1 ), the fixing base 761 is fixedly mounted on the second housing 71, and the lifting platform 763 is connected to the third calibration rail 764 (see FIG. 1 ). Fig.32 ) can be slidably mounted on the fixed seat 761, and the third calibration drive assembly 762 is fixedly mounted on the lifting platform 763. The third calibration drive assembly 762 has a power output end ( Fig.31 The power output end is connected to the fixed seat 761. When the third calibration drive assembly 762 moves, the lifting platform 763 is driven to rise and fall relative to the fixed seat 761.

[0321] like Fig.30 and Fig.31 As shown, the lifting platform 763 includes a first calibration slope 7631 and a second calibration slope 7632 . The first calibration slope 7631 faces the first direction X and is connected to the first calibration movable block 72 , and the second calibration slope 7632 faces the second direction Y and is connected to the second calibration movable block 73 .

[0322] Fig.32 FIG. 7 shows a schematic diagram of the first calibration slope 7631 and the first calibration cam follower 743. Fig.32As shown, at this time, the lifting platform 763 is in a low position, and the first calibration cam follower 743 is located on the upper part of the first calibration inclined surface 7631, so that the first calibration movable block 72 is located relatively close to the placement area, and this position is defined as the calibration position of the first calibration movable block 72. As the lifting platform 763 gradually moves upward, the first calibration inclined surface 7631 will push the first calibration cam follower 743 to gradually move to the right, so that the first calibration movable block 72 is away from the placement area, and the first calibration movable block 72 moves from the calibration position to the non-calibration position.

[0323] Fig.33 FIG. 7 shows a schematic diagram of the second calibration slope 7632 and the second calibration cam follower 753. Fig.33 As shown, at this time, the lifting platform 763 is in a low position, and the second calibration cam follower 753 is located on the upper part of the second calibration inclined surface 7632, so that the second calibration movable block 73 is located relatively close to the placement area, and this position is defined as the calibration position of the second calibration movable block 73. As the lifting platform 763 gradually moves upward, the second calibration inclined surface 7632 will push the second calibration cam follower 753 to gradually move to the left, so that the second calibration movable block 73 is away from the placement area, and the second calibration movable block 73 moves from the calibration position to the non-calibration position.

[0324] That is to say, when the lifting platform 763 is in a low position, the first calibration movable block 72 is connected to the upper part of the first calibration inclined surface 7631, and the second calibration movable block 73 is connected to the upper part of the second calibration inclined surface 7632, and the first calibration movable block 72 and the second calibration movable block 73 are both located in a calibration position relatively close to the placement area; when the lifting platform 763 is in a high position, the first calibration movable block 72 is connected to the lower part of the first calibration inclined surface 7631, and the second calibration movable block 73 is connected to the lower part of the second calibration inclined surface 7632, and the first calibration movable block 72 and the second calibration movable block 73 are both located in a non-calibration position relatively far away from the placement area.

[0325] like Fig.32 As shown, in order to ensure that the first calibration cam follower 743 can always abut against the first calibration inclined surface 7631, a first calibration elastic element 77 is connected to the first calibration mounting seat 74. The first calibration elastic element 77 applies a force toward the first calibration inclined surface 7631 to the first calibration mounting seat 74, thereby making the first calibration cam follower 743 abut against the first calibration inclined surface 7631.

[0326] Similar, such as Fig.33As shown, in order to ensure that the second calibration cam follower 753 can always abut against the second calibration inclined surface 7632, a second calibration elastic element 78 is connected to the second calibration mounting seat 75. The second calibration elastic element 78 applies a force toward the second calibration inclined surface 7632 to the second calibration mounting seat 75, thereby making the second calibration cam follower 753 abut against the second calibration inclined surface 7632.

[0327] The use process of the calibration device 7 is as follows:

[0328] When the position of the consumable box 200 needs to be calibrated, the third calibration drive assembly 762 drives the lifting platform 763 to move upward, so that the first calibration inclined surface 7631 and the second calibration inclined surface 7632 both move upward. Under the action of the first calibration inclined surface 7631, the first calibration movable block 72 is away from the placement area, and under the action of the second calibration inclined surface 7632, the second calibration movable block 73 is away from the placement area. At this time, the clamping mechanism 62 can easily place the consumable box 200 in the placement area. After the consumable box 200 is placed on the placement surface 711 (the transmission can be used), The sensor is used to detect to confirm whether the consumable box 200 is placed on the placement surface 711), and the third calibration drive component 762 drives the lifting platform 763 to move downward. At this time, the first calibration movable block 72 moves toward the placement area under the action of the first calibration elastic element 77, and the second calibration movable block 73 moves toward the placement area under the action of the second calibration elastic element 78. Then, the consumable box 200 is pushed toward the first calibration fixed block 712 by the first calibration movable block 72, and is pushed toward the second calibration fixed block 713 by the second calibration movable block 73, thereby realizing the calibration of the position of the consumable box 200.

[0329] The specific structure of the pushing device 4 is described in detail below.

[0330] like Fig.34 and Fig.35 As shown, the pushing device 4 includes:

[0331] The material pushing base 41 is connected to the first housing 100 , and a material pushing slide rail 411 and a material pushing driving assembly 44 are provided on the material pushing base 41 ;

[0332] The supporting platform 43 is slidably connected to the material pushing slide rail 411 and is used to support the consumable box 200;

[0333] The material pushing driving assembly 44 drives the supporting platform 43 to move along the material pushing slide rail 411 through the material pushing transmission assembly 45, so that the supporting platform 43 enters and exits the first housing 100 through the opening 1014;

[0334] The pushing assembly 46 is connected to the supporting platform 43 and is used to push the consumable box 200 away from the supporting platform 43 .

[0335] Among them, Fig.34The state of the pusher device 4 shown is the extended state. In this state, the support platform 43 moves along the pusher slide rail 411 to one end of the pusher slide rail 411 under the drive of the pusher drive assembly 44. The support platform 43 extends out of the gene sequencing library preparation instrument from the opening 1014, and the pusher assembly 46 is in the state of pushing out the consumable box 200. At this time, the consumable box 200 has been pushed away from the support platform 43. Fig.35 The pushing device 4 is in a retracted state. In this state, the supporting platform 43 is driven by the pushing drive assembly 44 to move along the pushing slide 411 to the other end of the pushing slide 411. The supporting platform 43 is retracted into the gene sequencing library preparation instrument, and the pushing assembly 46 is in a retracted state. At this time, the consumable box 200 that needs to be output from the outlet can be placed on the supporting platform 43.

[0336] Optionally, the supporting platform 43 includes a material pushing slider 432, and the material pushing slider 432 is slidably connected to the material pushing slide rail 411. In this way, the supporting platform 43 can stably reciprocate along the material pushing slide rail 411 under the drive of the material pushing drive assembly 44.

[0337] Optionally, the pusher transmission assembly 45 includes a screw and a nut threadedly connected to the screw, the screw is rotatably connected to the pusher base 41, the output end of the pusher drive assembly 44 is connected to the screw, and the support platform 43 is connected to the nut. The screw rotates, the nut slides along the screw, and then drives the support platform 43 to reciprocate. Optionally, the pusher drive assembly 44 can be a motor, and the shaft of the motor is connected to the screw to drive the rotation.

[0338] The shaft of the motor is connected to one end of the screw rod, so that the rotation of the motor drives the screw rod to rotate, and then converts the rotary motion of the motor into the linear motion of the nut. Therefore, the push drive assembly 44 can drive the support platform 43 to move along the push slide rail 411, that is, the support platform 43 moves to the end of the push slide rail 411 away from the opening 1014 of the gene sequencing library preparation instrument ( Fig.35 ) or the supporting platform 43 moves to one end of the pushing slide rail 411 close to the opening 1014 of the gene sequencing library preparation instrument ( Fig.34 status shown in ).

[0339] Optionally, the pushing assembly 46 includes an electric push rod 461 and a pushing head 462 , and the pushing head 462 is connected to the output end of the electric push rod 461 .

[0340] Optionally, the supporting platform 43 is provided with a strip hole 431 , which is parallel to the electric push rod 461 ; the electric push rod 461 is connected below the supporting platform 43 , and the push head 462 passes through the strip hole 431 from below and extends out of the upper surface of the supporting platform 43 .

[0341] The electric push rod 461 is arranged below the supporting platform 43, so that the pushing device 4 has a compact structure. By opening a strip hole 431 on the supporting platform 43, the push head 462 can extend out of the upper surface of the supporting platform 43, thereby pushing the consumable box 200 away from the supporting platform 43.

[0342] Optionally, the supporting platform 43 includes limiting protrusions 434 disposed on both sides of the upper surface of the supporting platform 43 , and the limiting protrusions 434 are in the shape of long strips and parallel to the electric push rod 461 .

[0343] There may be two limit protrusions 434, which are respectively arranged on both sides of the supporting platform 43. In this way, when the electric push rod 461 is retracted, the limit protrusion 434 can limit the displacement of the consumable box 200 together with the push head 462, and the limit protrusion 434 is in a long strip shape and parallel to the electric push rod 461, and can control the push direction of the consumable box 200 when the electric push rod 461 is pushed out.

[0344] Optionally, the pushing device 4 also includes a position sensor 471 and a positioning member 472. The position sensor 471 is arranged on the side of the pushing base 41, and the positioning member 472 is correspondingly arranged on the side of the supporting platform 43. When the supporting platform 43 moves to the end of the pushing base 41 away from the pushing direction, the position sensor 471 can detect the positioning member 472.

[0345] Among them, the pushing device 4 is in a retracted state when the gene sequencing library preparation instrument is turned on, or after the pushing device 4 completes docking with the external device, it will return from the extended state to the retracted state. The position sensor 471 is arranged on the side of the pushing base 41, and the positioning member 472 is correspondingly arranged on the side of the supporting platform 43. The position of the position sensor 471 and the position of the positioning member 472 are arranged so that when the pushing device 4 is in a retracted state, that is, when the supporting platform 43 moves along the pushing slide rail 411 to the end of the pushing base 41 away from the pushing direction, the positioning member 472 can be detected by the position sensor 471. Therefore, if the positioning member 472 is detected by the position sensor 471, it means that the pushing device 4 is in a retracted state. The position sensor 471 can be an infrared proximity sensor or a micro switch, and the positioning member 472 can be as follows. Fig.34 and Fig.35 The structure is arranged in a sheet shape.

[0346] like Figure 1 and Figure 36-Figure 47 As shown, the specific structure of the automatic door 3 is introduced below.

[0347] like Figure 1 and Fig.36As shown, the automatic door 3 provided in this embodiment is rotatably connected to the first shell 100, and is used to open or close the opening 1014, so as to prevent dust and the like from entering the first shell 100 from the opening 1014. The automatic door 3 is connected to the first shell 100 by rotation, so only one set of door driving mechanism 35 is needed to drive the door body 32 to close and open the opening 1014, thereby simplifying the structure of the gene sequencing library preparation instrument, reducing the space occupied by the automatic door 3 and reducing the cost of the automatic door 3.

[0348] like Figure 1 As shown, optionally, the box wall 101 includes a metal frame 1011 and a transparent plate 1012, and the metal frame 1011 is provided with a perspective window 1013 (such as Fig.38 As shown), the transparent plate 1012 is connected to the inner side of the metal frame 1011 and covers the perspective window 1013, so that the situation inside the first housing 100 can be easily observed through the transparent plate 1012.

[0349] like Figure 1 , Fig.36 , Fig.38 As shown, the automatic door 3 is approximately located at the lower part of the first housing 100. Of course, in other optional embodiments, the position of the opening 1014 and the automatic door closing can be set as needed.

[0350] like Figure 37-Figure 41 As shown, the automatic door 3 provided in this embodiment includes a door frame 31, a door body 32, a door connector 33, a fixed adjustment component 34 and a door driving mechanism 35.

[0351] like Fig.37 and Fig.38 As shown, the door frame 31 is connected to the inner side of the first housing 100, the door frame 31 is annular, and the inner edge 312 of the door frame 31 is opposite to the opening 1014. In this embodiment, the opening 1014 is rectangular, and the door frame 31 and the door body 32 are also rectangular, that is, the annular structure is a rectangular ring. Of course, in other embodiments, the opening 1014 can also be other shapes.

[0352] like Fig.37 and Fig.40 As shown, the door connector 33 includes a first connector 331, a second connector 332 and a third connector 333 which are connected in sequence in a U shape. The door connector 33 has a simple structure, is easy to process and controls the processing accuracy. One end of the first connector 331 is connected to the inner side of the door body 32 through a fixed adjustment component 34. The fixed adjustment component 34 can fine-tune its relative position with the door body 32 and is detachably connected to the inner side of the door body 32.

[0353] like Fig.39 and Fig.41As shown, the door driving mechanism 35 is connected to one end of the third connecting portion 333, so that the door body 32 is located at a closed position that fits the inner edge 312 and an open position that opens the opening 1014. When the door body 32 is located at the closed position, the door body 32 is located in the opening 1014. Optionally, the door driving mechanism 35 drives the door connecting member 33 to rotate at least 90°.

[0354] like Fig.44 As shown, the lower frame 311 of the door frame 31 is provided with an avoidance groove 3111, and the door body 32 is located at Fig.39 In the open position shown, the third connection portion 333 is at least partially located in the avoidance groove 3111 ; the first connection portion 331 , the second connection portion 332 and the third connection portion 333 form an avoidance space 334 for avoiding the lower frame 311 .

[0355] In this embodiment, the upper end of the door body 32 rotates from top to bottom to open the opening 1014, and the door frame 31 may also include an upper frame 315, a first side frame 316 and a second side frame 317. The upper frame 315 is located on the upper side of the lower frame 311, and the upper frame 315, the first side frame 316, the lower frame 311 and the second side frame 317 are connected in sequence to form an annular structure. Specifically, the annular structure is a rectangular ring to cooperate with the rectangular opening 1014.

[0356] In other optional embodiments, the lower end of the door body 32 rotates from bottom to top to open the opening 1014. At this time, the upper frame 315 is located at the lower side of the lower frame 311. Therefore, the upper and lower here are not limitations on specific positions.

[0357] Since one end of the first connecting portion 331 is connected to the inner side of the door body 32 through the fixed adjusting component 34, the fixed adjusting component 34 can fine-tune its relative position with the door body 32. Therefore, when the door body 32 is in a closed position, or due to processing errors or installation errors, the door body 32 cannot fit the inner edge 312 of the door frame 31, the position of the door body 32 can be fine-tuned by adjusting the connection position between the door body 32 and the fixed adjusting component 34, so that the door body 32 can completely fit the inner edge 312 of the door frame 31, thereby avoiding the situation where the door body 32 is not closed tightly.

[0358] Since the door frame 31 is connected to the inner side of the first shell 100, the door frame 31 is annular in structure, and the inner edge 312 of the door frame 31 is opposite to the opening 1014, when the door body 32 closes the opening 1014, the door body 32 fits to the inner edge 312 of the door frame 31, and the door frame 31 and the door body 32 cooperate to completely close the opening 1014, and the door body 32 is located in the opening 1014, that is, the door body 32 is embedded in the box wall 101, and the door body 32 does not protrude from the box wall 101, so that the integrity of the first shell 100 is stronger.

[0359] The door driving mechanism 35 is connected to one end of the third connecting portion 333, and the door body 32 and the door connecting member 33 rotate around one end of the third connecting portion 333 to close and open the opening 1014. Therefore, the automatic door 3 is connected to the first housing 100 by rotation, and only one set of door driving mechanism 35 is needed to drive the door body 32 to close and open the opening 1014, thereby simplifying the structure of the gene sequencing library preparation instrument, reducing the space occupied by the automatic door 3, and reducing the cost of the automatic door 3.

[0360] The first connection part 331, the second connection part 332 and the third connection part 333 form an escape space 334 for escaping the lower frame 311, so that the door connecting member 33 will not interfere with the box wall 101 and the door frame 31 during the rotation of the door body 32 between the open position and the closed position, so that the door body 32 and the door connecting member 33 can rotate at least 90°, so when the door body 32 is in the open position, it will not interfere with the pushing device 4. In addition, since the door body 32 and the door connecting member 33 rotate around one end of the third connection part 333, the door body 32 is at a distance from one end of the third connection part 333, so in the process of the door body 32 switching from the closed position to the open position, the door body 32 will gradually move away from the box wall 101, so the door body 32 will not interfere with the box wall 101, so the door body 32 can be closed smoothly.

[0361] The lower frame 311 of the door frame 31 is provided with an avoidance groove 3111. When the door body 32 is in the open position, the third connection portion 333 is at least partially located in the avoidance groove 3111. Therefore, when the automatic door 3 is installed, the automatic door 3 as a whole can be closer to the box wall 101, saving the space inside the first shell 100, making the automatic door 3 occupy less space, and the structure of the first shell 100 more compact. At the same time, it can also ensure that the strength of the door connection member 33 is not affected.

[0362] The automatic door 3 can prevent dust and the like from entering the first shell 100 from the opening 1014. The structure of the automatic door 3 reduces the space occupied by the automatic door 3, reduces costs, ensures that the opening of the door body 32 is not less than 90°, and that the door body 32 closes the opening 1014 and does not protrude from the first shell 100, thereby giving the first shell 100 a better overall feel.

[0363] In a specific embodiment, when the door body 32 is switched from the open position to the closed position, the door driving mechanism 35 drives the door connecting member 33 to rotate 90°, and the door body 32 also rotates 90° accordingly. Figure 1 and Fig.42 As shown, when the door body 32 is in the closed position, the door body 32 is vertically arranged, and the door body 32 and the box wall 101 are both vertically arranged, closing the opening 1014. Fig.36 and Fig.43As shown, when the door body 32 is in the open position, the door body 32 is horizontally arranged. On the one hand, the door body 32 will not interfere with the pushing device 4. On the other hand, relative to the obtuse angle between the door body 32 and the vertical direction, when the door body 32 is stationary in a horizontal arrangement, the force on the door connecting member 33, especially the connection between the third connecting part 333 and the door driving mechanism 35 is relatively small, thereby avoiding deformation of the door connecting member 33 and further ensuring the door body 32.

[0364] like Fig.37 and Fig.40 As shown, the fixing and adjusting assembly 34 includes a fixing bar 341, a fixing screw 342, a fixing nut 343 and an adjusting gasket. At least one end of the fixing bar 341 is provided with a U-shaped fixing groove 3411. The fixing bar 341 is connected to one end of the first connecting portion 331. Optionally, the fixing bar 341 and the door connecting member 33 are an integral structure. The fixing screw 342 is fixedly connected to the inner side of the door body 32. The fixing screw 342 is inserted into the U-shaped fixing groove 3411 and can move relative to the U-shaped fixing groove 3411, that is, the size of the U-shaped fixing groove 3411 is larger than the size of the fixing screw 342, and thus when the fixing screw 342 is inserted into the U-shaped fixing groove 3411, the relative position between the door body 32 and the fixing bar 341 can be finely adjusted. The fixing nut 343 can be threadedly connected to the fixing screw 342, and the fixing bar 341 is sandwiched between the door body 32 and the fixing nut 343, so that the door body 32 and the fixing bar 341 are fixed. The adjusting gasket is sleeved on the fixing screw 342, and the number of the adjusting gaskets is multiple, and can be selectively arranged between the fixing nut 343 and the fixing bar 341. Optionally, no adjusting gasket may be placed between the fixing nut 343 and the fixing bar 341, or one or more adjusting gaskets may be placed. The adjusting gasket may be a metal gasket or an elastic gasket such as rubber.

[0365] For example, when the door body 32 needs to be adjusted up, down, left, or right relative to the opening 1014, the relative positions of the fixing screw 342 and the U-shaped fixing groove 3411 can be adjusted accordingly. When the door body 32 is closed and the door body 32 is not close to the door frame 31, the adjustment gasket between the fixing nut 343 and the fixing strip 341 can be removed.

[0366] Optionally, both ends of the fixing strip 341 are provided with U-shaped fixing grooves 3411, the number of the door connectors 33 is two, and the direction in which the two door connectors 33 are sequentially arranged is perpendicular to the direction in which the two U-shaped fixing grooves 3411 on the same fixing strip 341 are sequentially arranged, that is, a total of four U-shaped fixing grooves 3411 and four fixing screws 342 are provided, the four U-shaped fixing grooves 3411 are located at the four corners of the rectangle, the four fixing screws 342 are located at the four corners of the rectangle, and each fixing screw 342 can be inserted into a U-shaped fixing groove 3411. When the door body 32 is tilted relative to the door frame 31, the number of adjustment gaskets sleeved on one or more fixing screws 342 can be adjusted as needed, so that the door body 32 is completely fitted with the inner edge 312 of the door frame 31. It can be understood that, only one fixing screw 342 is shown in the figure as being connected to a fixing nut 343, and in fact, each fixing screw 342 is connected to a fixing nut 343.

[0367] like Fig.45 As shown, the side of the door frame 31 close to the door body 32 is an abutting surface 313, and an abutting groove 314 is provided on the outer edge of the abutting surface 313. It can be understood that the inner edge 312 is a part of the abutting surface 313. The outer edge of the abutting surface 313 is relative to the inner edge 312 of the door frame 31, and the inner and outer are relative to the inner and outer rings of the annular structure. The position close to the inner ring of the annular structure is the inner edge 312, and the position close to the outer ring of the annular structure is the outer edge.

[0368] In an optional embodiment, the inner edge 312 is connected with an elastic sealing part, and the door body 32 can be closely attached to the elastic sealing part. The elastic sealing part can be a silicone pad or foam, etc., which is connected to the inner edge 312 by bonding or other means to enhance the sealing and fit between the door frame 31 and the door body 32, thereby improving the sealing effect.

[0369] like Fig.46 and Fig.47 As shown, the transparent plate 1012 is connected to the inner side of the metal frame 1011, the door body 32 and the metal frame 1011 abut on the abutting surface 313, and the transparent plate 1012 is arranged in the abutting groove 314 and abuts against the groove bottom of the abutting groove 314, thereby avoiding the appearance of a gap between the box wall 101 and the door frame 31, and avoiding the appearance of a gap between the door frame 31 and the door body 32. In this embodiment, since the transparent plate 1012 is connected to the inner side of the metal frame 1011, the door body 32 does not protrude from the metal frame 1011, but slightly protrudes from the transparent plate 1012. As a whole, the door body 32 does not protrude from the box wall 101.

[0370] like Fig.39 and Fig.41As shown, the door driving mechanism 35 includes a door driving member 351 and a door shaft 352. The door driving member 351 is used to drive the door shaft 352 to rotate. One end of the third connecting portion 333 is connected to the door shaft 352, that is, the door body 32 and the door connecting member 33 realize fixed-axis rotation through the door shaft 352. The door driving member 351 can be a stepping motor or the like.

[0371] The door drive mechanism 35 also includes a door drive transmission assembly, which includes a door drive driving pulley 355, a door drive driven pulley 356 and a door drive belt 357. The door drive driving pulley 355 is connected to the output end of the door drive member 351; the door drive driven pulley 356 is connected to one end of the door shaft 352; and the door drive belt 357 is sleeved outside the door drive driving pulley 355 and the door drive driven pulley 356. The door drive transmission assembly can make the output end of the door drive member 351 not coaxial with the door shaft 352, so as to facilitate the installation of the door drive member 351.

[0372] To facilitate fixing of the door driving member 351, optionally, the door driving mechanism 35 may further include a door driving first fixing portion 353, which is connected to the door driving member 351 and can be connected to the first housing 100 via connecting members such as bolts.

[0373] To facilitate fixing the door shaft 352, the door drive mechanism 35 may optionally further include a door drive second fixing portion 354, which is rotatably connected to the door shaft 352 and can be connected to the first housing 100 via a bolt or other connecting member. In this embodiment, four door drive second fixing portions 354 are arranged at intervals along the axial direction of the door shaft 352 to improve the stability of the door shaft 352.

[0374] In order to facilitate determination of the position of the door body 32 and the number of steps or angles of rotation of the door driving member 351, the automatic door 3 further comprises a door in-position detection member 36 for detecting whether the door body 32 is in a closed position or in an open position.

[0375] The door in position detection member 36 includes a door in position photoelectric sensor 361 and a door stopper 362. The door in position photoelectric sensor 361 is connected to the first housing 100. The door stopper 362 is connected to the door shaft 352. When the door body 32 is in the closed position or in the open position, the door stopper 362 is located between the receiving end and the transmitting end of the door in position photoelectric sensor 361. Fig.39 As shown, when the door body 32 is in the open position, the door stopper 362 is located between the receiving end and the transmitting end of the door in-position photoelectric sensor 361, and the door in-position detection member 36 can detect that the door body 32 is in position, and the number of steps or angle of rotation of the door driving member 351 is reset to zero. Fig.41As shown, when the door body 32 leaves the open position (including being in the closed position), the door stopper 362 is located outside the receiving end and the transmitting end of the door in place photoelectric sensor 361, and when the number of steps or angles rotated by the door driver 351 is 90°, the door driver 351 stops working and the door body 32 rotates into place (i.e., rotates to the closed position).

[0376] The working process of the gene sequencing library preparation instrument in this embodiment is roughly as follows:

[0377] The liquid transfer mechanism 63 of the transfer device 6 adsorbs the solutions such as reagents and samples on the cooling device 5, the heating device 1 and / or the constant temperature plate module into one of the consumable boxes 200 to form a reaction sample (at this time, the consumable box 200 carrying the reaction sample can be placed in one of the second constant temperature plate modules 92, but is not limited to the second constant temperature plate module 92);

[0378] The clamping and placing mechanism 62 of the transfer device 6 transfers the consumable box 200 carrying the reaction sample to the calibration device 7, and the calibration device 7 calibrates the position of the consumable box 200;

[0379] The clamping and placing mechanism 62 of the transfer device 6 transfers the consumable box 200 on the calibration device 7 to the shaking device 8, and shakes to mix the reaction sample;

[0380] The clamping mechanism 62 transfers the consumable box 200 on the shaking device 8 to the magnetic rack device 400, thereby purifying the reaction sample in the consumable box 200;

[0381] The liquid transfer mechanism 63 of the transfer device 6 absorbs the reaction sample in the consumable box 200 on the magnetic frame device 400 and transfers it to the consumable box 200 of the PCR amplification device 500, and the PCR amplification device 500 performs PCR amplification on the reaction sample;

[0382] The pipetting mechanism 63 of the transfer device 6 absorbs part of the reaction sample in the consumable box 200 on the PCR amplification device 500 (e.g., 5%-20% of the reaction sample is absorbed from each containing monomer) to another consumable box 200 (the other consumable box 200 is located in one of the second constant temperature plate modules 92, but not limited to the second constant temperature plate module 92, and may also be on other modules that can carry the consumable box 200), and the clamping mechanism 62 transfers the consumable box 200 containing the remaining reaction sample to one of the second constant temperature plate modules 92 (not limited to the second constant temperature plate module 92, and may also be on other modules that can carry the consumable box 200);

[0383] The clamping mechanism 62 transfers the other consumable box 200 to the fluorescence detection device 600, so as to detect the concentration of the object to be detected at the fluorescence detection device 600;

[0384] The clamping and placing mechanism 62 transfers another consumable box 200 at the fluorescence detection device 600 to the recycling module 300;

[0385] The pipetting mechanism 63 transfers the object to be detected to another consumable box 200 (the other consumable box 200 is located in one of the second constant temperature plate modules 92, but is not limited to the second constant temperature plate module 92, and may also be on other modules that can carry the consumable box 200), wherein the pipetting mechanism 63 can calculate the volume of the object to be detected that needs to be transferred to the other consumable box 200 according to the concentration value of the object to be detected detected by the fluorescence detection device 600;

[0386] The clamping mechanism 62 transfers the consumable box 200 on the magnetic frame device 400 to the recovery module 300 or the pushing device 4, and the pushing device 4 can transfer the consumable box 200 to the outside of the first shell 100; transfer the above-mentioned another consumable box 200 (containing the object to be detected) to the pushing device 4, and the pushing device 4 transfers the consumable box 200 to the outside of the first shell 100 to facilitate another device to receive the consumable box 200.

[0387] During the operation of the gene sequencing library preparation instrument, if it is necessary to transfer the consumable box 200 to other equipment or staff outside the gene sequencing library preparation instrument, the clamping mechanism 62 can place the consumables on the pushing device 4, and the pushing device 4 transports the consumable box 200 to the outside of the gene sequencing library preparation instrument.

[0388] When the solutions are not contaminated with each other, the pipetting mechanism 63 can reuse the pipette tip, thereby further increasing the detection throughput.

[0389] Although the above general description and specific implementation methods have been used, it is obvious to those skilled in the art that some modifications or improvements can be made based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A gene sequencing library preparation instrument, characterized in that: include: A first shell (100), wherein an opening (1014) is provided on a box wall (101) of the first shell (100); An automatic door (3) rotatably connected to the first housing (100), the automatic door (3) being capable of rotating at least 90° to open or close the opening (1014); A plurality of functional devices are arranged in the first housing (100) and are capable of placing consumable boxes (200), amplification reaction samples and / or fluorescence detection; A pushing device (4) is arranged in the first shell (100), and the pushing device (4) is capable of conveying the carried consumable box (200) to the outside of the first shell (100) through the opening (1014), and pushing the consumable box (200) away from the pushing device (4), and is capable of carrying the consumable box (200) outside the first shell (100), and conveying the consumable box (200) outside the first shell (100) into the first shell (100); The transfer device (6) is arranged in the first housing (100) and is capable of transferring the consumable box (200) and the solution between the plurality of functional devices and the pushing device (4).

2. The gene sequencing library preparation instrument according to claim 1, characterized in that: The automatic door (3) comprises a door frame (31), a door body (32), a door connecting member (33), a fixing and adjusting component (34) and a door driving mechanism (35); The door frame (31) is connected to the inner side of the first shell (100), the door frame (31) is an annular structure, and the inner edge (312) of the door frame (31) is directly opposite to the opening (1014); The door connecting member (33) comprises a first connecting portion (331), a second connecting portion (332) and a third connecting portion (333) which are connected in sequence in a U shape, one end of the first connecting portion (331) is connected to the inner side of the door body (32) through the fixed adjustment component (34), and the fixed adjustment component (34) can finely adjust its relative position with the door body (32) and is detachably connected to the inner side of the door body (32); The door driving mechanism (35) is connected to one end of the third connecting portion (333), so that the door body (32) is located at a closed position abutting against the inner edge (312) and an open position opening the opening (1014); when the door body (32) is located at the closed position, the door body (32) is located in the opening (1014); The lower frame (311) of the door frame (31) is provided with an avoidance groove (3111); when the door body (32) is located in the open position, the third connecting portion (333) is at least partially located in the avoidance groove (3111); the first connecting portion (331), the second connecting portion (332) and the third connecting portion (333) form an avoidance space (334) for avoiding the lower frame (311).

3. The gene sequencing library preparation instrument according to claim 2, characterized in that: The fixed adjustment component (34) comprises: A fixing bar (341), wherein at least one end of the fixing bar (341) is provided with a U-shaped fixing groove (3411); A fixing screw (342), wherein the fixing screw (342) is fixedly connected to the inner side of the door body (32), and the fixing screw (342) is inserted into the U-shaped fixing groove (3411) and is movable relative to the U-shaped fixing groove (3411); A fixing nut (343), wherein the fixing nut (343) can be threadedly connected to the fixing screw (342), and the fixing strip (341) is sandwiched between the door body (32) and the fixing nut (343); An adjusting gasket, wherein the adjusting gasket is in multiple numbers and can be selectively arranged between the fixing nut (343) and the fixing strip (341), and the adjusting gasket is sleeved on the fixing screw (342).

4. The gene sequencing library preparation instrument according to claim 1, characterized in that: The pushing device (4) comprises: A material pushing base (41) is connected to the first housing (100), and a material pushing slide rail (411) and a material pushing driving assembly (44) are provided on the material pushing base (41); A supporting platform (43) is slidably connected to the material pushing slide rail (411) and is used to support the consumables box (200); The push drive assembly (44) drives the supporting platform (43) to move along the push slide rail (411) through the push transmission assembly (45), so that the supporting platform (43) enters and exits the first housing (100) through the opening (1014); A material pushing assembly (46) is connected to the supporting platform (43) and is used to push the consumable box (200) away from the supporting platform (43).

5. The gene sequencing library preparation instrument according to claim 1, characterized in that: A plurality of said functional devices include: a cooling device (5), capable of carrying the consumable box (200) and cooling the consumable box (200); and / or A heating device (1) capable of carrying the consumable box (200) and heating the consumable box (200); and / or A normal temperature board module capable of carrying the consumables box (200) at normal temperature; and / or A recycling module (300) capable of recycling discarded consumables; and / or a calibration device (7) on which the position of the consumables box (200) can be calibrated; and / or an oscillating device (8) capable of mixing the solution; and / or A magnetic rack device (400) capable of enriching magnetic microspheres and / or A PCR amplification device (500), used for amplifying a reaction sample; and / or The fluorescence detection device (600) is used to detect the reaction sample after amplification.

6. The gene sequencing library preparation instrument according to claim 5, characterized in that: The cooling device (5) comprises: A cooling module (51), comprising a cooling base (52) connected to the first housing (100) and a cooling water drain (53) detachably arranged above the cooling base (52), wherein the cooling water drain (53) is used to cool the consumable box (200); A condensation water blocking module (54) comprises a cover plate support frame (55), a first cover plate (56), a second cover plate (59) and a cover plate driving unit (518); the cover plate support frame (55) is provided with a cooling shaft (57) connected to the first cover plate (56); the first cover plate (56) is fixedly connected to the second cover plate (59) via a cover plate elastic member (58); the cover plate driving unit (518) is connected to the cover plate support frame (55) and is used to drive the cooling shaft (57) to rotate so that the second cover plate (59) seals and covers the consumable box (200) or opens the consumable box (200); and / or The heating device (1) comprises: A heating base (11) is connected to the first housing (100) and is provided with a sink; A heating plate (12) is arranged in the sink of the heating base (11); A heating jig (13) is arranged on the heating base (11); a heating groove (132) is provided on the upper surface of the heating jig (13); the consumable box (200) is placed on the heating jig (13); a receiving unit of the consumable box (200) can be inserted into the heating groove (132) and contact the groove wall of the heating groove (132); and a ventilation structure (133) is provided on the groove wall of the heating groove (132); and / or The calibration device (7) comprises: A placement surface (711), wherein an upper portion of the placement surface (711) defines a placement area; A first calibration fixing block (712) is located at a first calibration side of the placement area in a first direction (X); a first calibration movable block (72) located at a second calibration side of the placement area in a first direction (X), the first calibration movable block (72) being configured to be movable along the first direction (X), wherein the second calibration side in the first direction (X) is arranged opposite to the first calibration side in the first direction (X); a second calibration fixing block (713), the second calibration fixing block (713) being located at a first calibration side of a second direction (Y) of the placement area, wherein the first direction (X) and the second direction (Y) are perpendicular to each other; and a second calibration movable block (73), the second calibration movable block (73) being located at a second calibration side of the placement area in a second direction (Y), the second calibration movable block (73) being configured to be movable along the second direction (Y), wherein the second calibration side in the second direction (Y) is arranged opposite to the first calibration side in the second direction (Y); and the first direction (X) is perpendicular to the second direction (Y); and / or The oscillating device (8) comprises: An oscillating working support (81) connected to the first housing (100); An oscillating motor assembly (82) and an oscillating eccentric wheel assembly (83), wherein the oscillating motor assembly (82) comprises an oscillating stepping motor (823) and a coupling assembly (824), and the oscillating stepping motor (823) is mounted on the oscillating working bracket (81); The output end of the oscillating stepping motor (823) is eccentrically arranged through the oscillating working bracket (81) and the oscillating eccentric wheel assembly (83), and both are connected to the coupling assembly (824); An oscillating tray (85) is connected to the oscillating eccentric wheel assembly (83), and the oscillating eccentric wheel assembly (83) is rotatable relative to the oscillating tray (85), and a detachable consumable box (200) is placed on the oscillating tray (85); An oscillation guide mechanism (84) is arranged between the oscillation working support (81) and the oscillation tray (85), the oscillation guide mechanism (84) is located on one side of the oscillation eccentric wheel assembly (83), and the oscillation guide mechanism (84) limits the oscillation tray (85) to move in a first direction (X) and a second direction (Y) in a horizontal plane; A reset assembly (86), comprising a first fixing member (863), an oscillation reset spring (864), and a crank (8653) connecting rod member (865), wherein the first fixing member (863) is arranged on the oscillation working support (81) or on the bottom of the oscillation tray (85); A crank (8653) connecting rod (865) connected to the coupling assembly (824) and capable of rotating relative to the coupling assembly (824), wherein the rotation center of the crank (8653) connecting rod (865) is not coaxial with the oscillating stepping motor (823) and / or the coupling assembly (824); One end of the oscillation return spring (864) is connected to the crank (8653) connecting rod (865), and the other end is connected to the first fixing member (863).

7. The gene sequencing library preparation instrument according to claim 6, characterized in that: The calibration device (7) further comprises: A calibration drive mechanism (76), the calibration drive mechanism (76) being configured to drive the first calibration movable block (72) and the second calibration movable block (73) to simultaneously approach or move away from the placement area, the calibration drive mechanism (76) comprising: A lifting platform (763), wherein the lifting platform (763) comprises: a first calibration inclined plane (7631), the first calibration inclined plane (7631) faces the first direction (X), and the first calibration inclined plane (7631) is connected to the first calibration movable block (72); and a second calibration inclined plane (7632), the second calibration inclined plane (7632) facing the second direction (Y), the second calibration inclined plane (7632) being connected to the second calibration movable block (73); Wherein, the lifting platform (763) has a high position and a low position; Wherein, when the lifting platform (763) is located at the low position, the first calibration movable block (72) is connected to the upper part of the first calibration inclined surface (7631), and the second calibration movable block (73) is connected to the upper part of the second calibration inclined surface (7632), and the first calibration movable block (72) and the second calibration movable block (73) are both located at a calibration position relatively close to the placement area; Wherein, when the lifting platform (763) is in the high position, the first calibration movable block (72) is connected to the lower part of the first calibration inclined surface (7631), and the second calibration movable block (73) is connected to the lower part of the second calibration inclined surface (7632), and the first calibration movable block (72) and the second calibration movable block (73) are both located in a non-calibration position relatively far away from the placement area.

8. The gene sequencing library preparation instrument according to claim 5, characterized in that: The gene sequencing library preparation instrument further comprises an anti-lifting device (2), the cooling device (5), the heating device (1) and / or the normal temperature plate position module are connected with the anti-lifting device (2), and the anti-lifting device (2) is used to fix the consumable box (200) located on the cooling device (5), the heating device (1) or the normal temperature plate position module; The anti-lifting device (2) comprises: A clamping and fixing component (21) which is fixedly arranged relative to the cooling device (5), the heating device (1) or the normal temperature plate module; A clamping and lifting assembly (23), comprising a clamping and lifting seat (231), wherein the clamping and lifting seat (231) is arranged to be lifted and lowered relative to the clamping and fixing assembly (21); A clamping linkage assembly (24), comprising a clamping abutment (241), a clamping linkage (242) and a clamping elastic member (243), wherein the clamping abutment (241) abuts against the clamping lifting seat (231), the clamping abutment (241) is connected to the clamping lifting seat (231) via the clamping elastic member (243), and one end of the clamping linkage (242) is rotatably connected to the clamping abutment (241); A clamping assembly (22), comprising a clamping jaw (221), wherein the clamping jaws (221) are symmetrically arranged on opposite sides of the consumable box (200) along the second direction (Y), and the clamping jaws (221) are arranged in a one-to-one correspondence with the clamping linkage assembly (24), and the other end of the clamping linkage (242) is coaxially fixedly connected to the clamping jaw (221), and each of the clamping jaws (221) can be rotatably arranged on the cooling device (5), the heating device (1) or the constant temperature plate module, so as to be able to clamp the consumable box (200) so that the consumable box (200) is pressed against the cooling device (5), the heating device (1) or the constant temperature plate module.

9. The gene sequencing library preparation instrument according to claim 5, characterized in that: The normal temperature panel module comprises a first normal temperature panel module (91), a plurality of second normal temperature panel modules (92) and a third normal temperature panel module (93); The cooling device (5), the first constant temperature panel module (91), the heating device (1) and at least one of the second constant temperature panel modules (92) are arranged in sequence along a second direction (Y) to form a first row of functional areas; The PCR amplification device (500), the magnetic frame device (400) and the recovery module (300) are arranged in sequence along the second direction (Y) to form a second column of functional areas; The fluorescence detection device (600) and at least one of the second constant temperature plate modules (92) are arranged in sequence along the second direction (Y) to form a third column of functional areas; At least one of the second constant temperature plate position modules (92), the oscillation device (8) and the calibration device (7) are arranged in sequence along the second direction (Y) to form a fourth column of functional areas; The pushing device (4) and the third normal temperature plate position module (93) are arranged in sequence along the second direction (Y) to form a fifth row of functional areas; The first column of functional areas, the second column of functional areas, the third column of functional areas, the fourth column of functional areas and the fifth column of functional areas are sequentially arranged along a first direction (X).

10. The gene sequencing library preparation instrument according to claim 1, characterized in that: The transfer device (6) comprises: A clamping and placing mechanism (62) capable of clamping and placing the consumables box (200); A liquid transfer mechanism (63) capable of transferring the solution in the consumable box (200); A three-way driving mechanism (61) is connected to the top of the first housing (100), and the three-way driving mechanism (61) is used to drive the clamping mechanism (62) and the liquid transfer mechanism (63) to move in the horizontal direction and the vertical direction.

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