Chest and ascites cell frozen section and fluorescent probe dyeing integrated device
By combining low-temperature collection and P80 fluorescent probe recognition technology with an automated processing system, the problems of cell degeneration and low sensitivity of HE staining in pleural and peritoneal fluid cell detection have been solved, achieving efficient and accurate detection of malignant tumor cells.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-21
- Publication Date
- 2026-03-31
AI Technical Summary
Existing technologies for detecting cells in pleural and peritoneal fluid are hampered by the complexity of cell composition, the time-consuming collection and processing leading to cell degeneration and death, and the low sensitivity of HE staining methods, resulting in a high risk of misdiagnosis and missed diagnosis.
The system uses low-temperature collection bottles to preserve pleural and peritoneal fluid samples and utilizes P80 fluorescent probes to identify misfolded proteins. It integrates components such as an automated conveyor system, a cooling module, and an electric push rod to achieve automated sample processing and highly sensitive staining.
It maintains cell viability for 24 hours, improves detection coverage and accuracy, reduces the rate of missed diagnoses, enables the processing of large numbers of samples at once, and enhances the ability to identify malignant tumor cells.
Smart Images
Figure CN224066472U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of medical device technology, and in particular to an integrated device for frozen sectioning of pleural and peritoneal cells and staining with fluorescent probes. Background Technology
[0002] Clinically, pleural and peritoneal effusions can be classified as benign or malignant. Malignant pleural and peritoneal effusions are a common complication of advanced malignant tumors. They are caused by the distant metastasis of malignant tumors to the pleura and peritoneum, leading to diffuse lesions of the pleura and peritoneum and an abnormal increase in body cavity fluid. Pleural and peritoneal cytological smears or pleural and peritoneal biopsies are the standard methods for diagnosing malignant pleural and peritoneal effusions. Pleural and peritoneal biopsies require laparoscopic surgery under local anesthesia, which is not widely accepted in clinical practice. Therefore, multiple pleural and peritoneal cytological examinations are considered the gold standard for diagnosis in clinical practice.
[0003] Meanwhile, while routine HE staining of cell smears has some value in determining the nature of cells in pleural effusions, the complex cellular composition and similar morphological characteristics of various cells in pleural and peritoneal fluid can easily lead to misdiagnosis. Current techniques typically require the collection of large amounts of pleural and peritoneal fluid, which is not only time-consuming but also prone to cell degeneration and death during the collection process, leading to missed diagnoses. Secondly, after the specimens are delivered to the pathology department, the cell sedimentation method is often used to collect the cell sediment, which takes a long time and further aggravates cell death, increasing the rate of missed diagnoses. In addition, the cytological detection methods currently used, which rely on HE staining, have low sensitivity and require repeated testing to increase the detection rate. Utility Model Content
[0004] To overcome the problems of conventional cell smear HE staining in pleural and peritoneal fluid cell detection, such as complex cell composition, time-consuming collection and processing leading to cell degeneration and death, low method sensitivity requiring multiple tests, and high risk of misdiagnosis and missed diagnosis, this utility model provides an integrated device for pleural and peritoneal fluid cell frozen section and fluorescent probe staining.
[0005] The technical solution is as follows: A device integrating frozen sectioning and fluorescent probe staining of pleural and peritoneal fluid cells includes a mounting frame, a support pad, a detection frame, a storage tank, an inlet, a slicing blade, an electric push rod, a detection module, a mounting bracket, a control valve, a probe staining solution output tube, a reaction termination solution output tube, and a control board; a support pad is mounted around the outer side of the upper surface of the mounting frame, and a detection frame is located at the center of the upper end of the mounting frame. An electric push rod for slicing pleural and peritoneal fluid cells is mounted inside the upper rear end of the detection frame, and a slicing blade is located at the piston end of the electric push rod. Mounting brackets are located at the center and front end of the inner side of the detection frame, and a control module is located at the center and front end of the mounting bracket for controlling... The system has two sets of flow control valves, each with a reservoir at its upper end. The reservoir has an inlet at the center of its upper surface. The lower surface of the control valve inside the reservoir has a probe staining solution output tube for binding to proteins, locating and visualizing biomolecules in the sample. The lower surface of the control valve on the inner front end has a reaction termination solution output tube for stopping specific biochemical reactions to prevent distorted results due to over-reaction. The front end of the detection frame has a control board electrically connected to the control valves. The upper inside of the detection frame, at the front end of the reaction termination solution output tube, the probe staining solution output tube, and the electric push rod, are all equipped with detection modules electrically connected to the control board.
[0006] Furthermore, a cold stage is provided on the upper surface of the mounting frame at the rear end of the detection frame, an insulated container is provided in the center of the cold stage, and a third magnetic block is provided inside the lower surface of the insulated container.
[0007] Furthermore, a sliding groove is provided on the side of the cold stage near the detection frame, and a cooling module electrically connected to the control board is installed at the center of the lower surface of the cold stage. A heat dissipation groove corresponding to the cooling module is provided on the side surface of the mounting frame.
[0008] Furthermore, a conveyor belt is provided in the cavity on the upper surface of the mounting frame. A rotating column connected to the mounting frame is provided inside the end of the conveyor belt near the cold table. A transmission column is provided on the outer side of the rear end of the conveyor belt. A transmission module electrically connected to the control board is provided on the lower surface of the conveyor belt.
[0009] Furthermore, a support frame is installed at the lower rear end of the conveyor belt, and a motor is installed at the upper end of the support frame. The output end of the motor is equipped with a transmission belt that meshes with the transmission column.
[0010] Furthermore, a second magnetic block corresponding to the third magnetic block is provided at the center of the upper surface of the conveyor belt, and a placement plate is fixedly connected to the center of the front end of the support pad.
[0011] Furthermore, a first magnetic block corresponding to the second magnetic block is provided at the center of the upper surface of the placement plate, and a push-pull plate is provided on the side surface of the mounting frame, with a handle installed at the center of the surface of the push-pull plate.
[0012] Furthermore, a fixing plate is provided on one side of the upper surface of the mounting frame located on the cold table. A robotic arm electrically connected to the control board is installed at the center of the upper surface of the fixing plate. A vacuum pump is clamped and installed at the piston end of the robotic arm, and a negative pressure suction head is installed at the output end of the vacuum pump.
[0013] The beneficial effects are as follows: This invention enables the identification of misfolded proteins in malignant tumor cells using a P80 fluorescent probe. Unlike traditional methods that rely on differences in cell morphology for identification, the P80 probe emits strong fluorescence specifically targeting misfolded, denatured, and aggregated proteins, exhibiting high sensitivity and specificity. This method can more accurately distinguish between malignant tumor cells and normal cells. By using a low-temperature collection bottle that can be stored at 0-4℃, cell viability is maintained within 24 hours after puncture, ensuring analysis within the optimal detection period. It can process large quantities (600-1000ml) of pleural and peritoneal fluid samples at once, compared to existing technologies that can only collect small quantities (50-100ml) of samples, greatly increasing the detection coverage, helping to discover more potential problems, and reducing the rate of missed diagnoses. Attached Figure Description
[0014] Figure 1 This is a three-dimensional structural diagram of an integrated device for frozen sectioning and fluorescent probe staining of pleural and peritoneal fluid cells according to the present invention.
[0015] Figure 2 This is a schematic diagram of the liquid storage tank structure of this utility model;
[0016] Figure 3 This is a schematic diagram of the conveyor belt structure of this utility model;
[0017] Figure 4 This is a schematic diagram of the slicing blade structure of this utility model;
[0018] Figure 5 This is a schematic diagram of the refrigeration module structure of this utility model.
[0019] In the attached diagram, the following are the reference numerals: 1. Mounting frame; 2. Support pad; 3. Heat dissipation groove; 4. Fixing plate; 5. Robotic arm; 6. Vacuum pump; 7. Negative pressure suction head; 8. Cold stage; 9. Push-pull plate; 10. Handle; 11. Detection frame; 12. Storage tank; 13. Inlet; 14. Placement plate; 15. First magnetic block; 16. Conveyor belt; 17. Second magnetic block; 18. Rotating column; 19. Transmission column; 20. Transmission belt; 21. Motor; 22. Transmission module; 23. Support frame; 24. Slicing blade; 25. Electric push rod; 26. Detection module; 27. Mounting frame; 28. Control valve; 29. Probe staining solution output tube; 30. Reaction termination solution output tube; 31. Sliding groove; 32. Insulated container; 33. Third magnetic block; 34. Refrigeration module; 35. Control board. Detailed Implementation
[0020] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.
[0021] Among the currently discovered feasible technologies, the following are described:
[0022] Clinically, pleural effusion and ascites are mainly classified based on their causes and properties, and can be divided into benign and malignant pleural effusions. Malignant pleural effusion, as a common complication of advanced malignant tumors, is usually caused by the spread of primary or metastatic malignant tumor cells to the pleural or peritoneal region, leading to diffuse lesions in these areas and resulting in abnormal accumulation of fluid in the body cavities. This pathological state not only seriously affects the patient's quality of life but may also be an important marker of disease progression. In terms of diagnosis, the main methods currently used to differentiate between benign and malignant pleural effusion and ascites include pleural and peritoneal cytological smear examination and pleural and peritoneal biopsy. The former involves aspirating fluid from the pleural or peritoneal cavity and performing detailed cytological analysis to look for the presence of malignant tumor cells. This method is widely used in clinical practice due to its simplicity, speed, and minimal invasiveness. In contrast, while pleural and peritoneal biopsy has higher accuracy, it requires laparoscopic techniques and surgery under local anesthesia to obtain tissue samples. Therefore, its acceptance is relatively low for elderly patients or individuals in poor physical condition who cannot tolerate surgery. With limitations, repeated pleural and peritoneal effusion cytology examinations have gradually become the "gold standard" for diagnosing malignant pleural and peritoneal effusions. This non-invasive testing method, through repeated collection of pleural and peritoneal fluid samples and meticulous cytological analysis, can significantly improve the accuracy and reliability of diagnosis. Furthermore, it allows doctors to monitor disease progression based on dynamic changes in the condition, adjust treatment plans in a timely manner, and provide patients with more personalized and precise treatment strategies. This method not only helps in the early detection of the disease but also promotes the implementation of early intervention measures, thereby improving patient prognosis and quality of life. With the development of medical technology, such as the application of integrated devices mentioned earlier, the efficiency and accuracy of pleural and peritoneal effusion cytology examinations will be further improved. These devices integrate multiple functions, from automated sample processing to advanced fluorescent probe staining technology, which not only improves the detection speed but also enhances the accuracy of results. This opens up new avenues for the diagnosis of malignant pleural and peritoneal effusions, and is expected to bring higher diagnostic value and play an important role in future clinical practice. Through these advancements, medical professionals will be able to manage such conditions more effectively and provide patients with more optimized treatment plans.
[0023] While routine hematoxylin and eosin (HE) staining of cell smears does have some value in determining the cellular composition of pleural effusions, its limitations cannot be ignored. The cellular components in pleural and peritoneal effusions are highly complex, and different cell types may exhibit similar morphological characteristics, making differentiation based solely on HE staining difficult and prone to misdiagnosis. Furthermore, current techniques require the collection of large amounts of pleural and peritoneal fluid during sample collection, a process that is not only time-consuming but also susceptible to cell degeneration or even death due to operational conditions and time constraints. This further increases the risk of missed diagnoses. Specifically, in practice, to ensure sufficient cell quantity for analysis, a relatively large amount of pleural and peritoneal fluid is typically collected; however, this practice is often accompanied by cell degeneration, especially during prolonged operation. During the process, cell viability is difficult to maintain, thus affecting the accuracy of the final diagnosis. After the specimen is sent to the pathology department, the traditional processing method is to collect cell pellets by static precipitation. Although this method is simple and easy to implement, it takes a long time to complete. This waiting process also leads to an increase in cell death rate, thereby increasing the possibility of missed diagnosis. Furthermore, current cytological testing mainly relies on HE staining. Although this method can provide basic cell structure information, it is not very sensitive. This means that HE staining may not be able to effectively identify some subtle changes or low concentrations of target cells. Therefore, it is often necessary to repeat the test to improve the detection rate. However, this approach not only increases the workload, but may also lead to a decline in sample quality due to repeated operations, affecting the final diagnostic results.
[0024] This device uses cryogenic collection bottles to preserve pleural and peritoneal fluid samples, maintaining sample freshness at 0-4°C and preserving cell viability for up to 24 hours after thoracentesis. This significantly reduces the risk of cell degeneration and death due to prolonged processing. Unlike traditional HE staining methods, this device utilizes P80 fluorescent probes to identify misfolded proteins in malignant tumor cells. This method not only more accurately distinguishes malignant tumor cells from normal cells but also exhibits high sensitivity and specificity, accurately detecting target molecules even at low concentrations. The device integrates an automated conveyor system, cooling module, and electric actuators, achieving one-stop automated processing from sample collection, cooling, and transfer to sectioning and staining. This not only improves work efficiency and reduces errors that may be caused by human operation but also ensures that each step is performed under optimal conditions, further enhancing the accuracy of the results.
[0025] like Figure 1 - Figure 5As shown, an integrated device for frozen sectioning and fluorescent probe staining of pleural and peritoneal fluid cells includes a mounting frame 1, a support pad 2, a detection frame 11, a storage tank 12, an inlet 13, a slicing blade 24, an electric push rod 25, a detection module 26, a mounting bracket 27, a control valve 28, a probe staining solution output tube 29, a reaction termination solution output tube 30, and a control plate 35. The support pad 2 is mounted around the outer side of the upper surface of the mounting frame 1. The detection frame 11 is located at the center of the upper end of the mounting frame 1. An electric push rod 25 for slicing pleural and peritoneal fluid cells is mounted inside the upper rear end of the detection frame 11. A slicing blade 24 is located at the piston end of the electric push rod 25. A mounting bracket 27 is located at both the center and front end of the inner side of the detection frame 11. A slicing blade 24 is located at the center and front end of the inner side of the mounting bracket 27. Two sets of control valves 28 for controlling flow are provided. Each control valve 28 is equipped with a storage tank 12 at its upper end. The storage tank 12 has an inlet 13 at the center of its upper surface. The control valve 28 at its inner center has a probe staining solution output tube 29 at its lower surface center for binding with proteins, locating and visualizing biomolecules in the sample. The control valve 28 at its inner front end has a reaction termination solution output tube 30 at its lower surface center for stopping specific biochemical reactions to prevent result distortion due to over-reaction. The detection frame 11 has a control plate 35 at its front end that is electrically connected to the control valves 28. The detection module 26, which is electrically connected to the control plate 35, is installed on the upper side of the detection frame 11 at the front end of the reaction termination solution output tube 30, the probe staining solution output tube 29 and the electric push rod 25.
[0026] A cold stage 8 is located on the upper surface of the mounting frame 1 at the rear end of the detection frame 11. An insulated container 32 is located at the center of the cold stage 8. A third magnetic block 33 is located inside the lower surface of the insulated container 32. The presence of the insulated container 32 and the third magnetic block 33 inside the cold stage 8 ensures that the sample maintains the required low temperature environment during the detection process, thereby improving the cell viability maintenance effect. A sliding groove 31 is provided on the side of the cold stage 8 near the detection frame 11. A cooling module 34 electrically connected to the control board 35 is installed at the center of the lower surface of the cold stage 8. A heat dissipation groove 3 corresponding to the cooling module 34 is provided on the side surface of the mounting frame 1. By installing the cooling module 34 electrically connected to the control board 35 and the corresponding heat dissipation groove 3, the temperature of the cold stage 8 is effectively maintained, ensuring that the sample quality is not affected by the external temperature.
[0027] During operation, a low-temperature high-speed centrifuge is first used to centrifuge at 0-5000 rpm in an environment of 0-4℃. The cell pellet adsorption probe then uses negative pressure to transfer the cell pellet from the bottom of the centrifuge tube to the cold stage 8 of the frozen section preparation system. The cold stage 8 first provides continuous cooling to the insulated container 32 through its internal cooling module 34, ensuring that the temperature remains stable within the set range. The insulated container 32 maintains the low temperature of the sample, which helps to improve the maintenance of cell viability. When the sample needs to be processed, the sliding groove 31 on the side of the cold stage 8 near the detection frame 11 allows the sample container to be smoothly moved to the conveyor belt 16 via the third magnetic block 33. To ensure that the cooling module 34 can work continuously and effectively, the side surface of the mounting frame 1 is provided with heat dissipation grooves 3 corresponding to the cooling module 34. These heat dissipation grooves 3 help dissipate the heat generated during the cooling process, avoiding overheating that affects the cooling efficiency, thereby effectively maintaining the temperature of the cold stage 8 and ensuring that the sample quality is not affected by changes in external temperature. Throughout the process, the control board 35 is responsible for monitoring and adjusting the working status of the cooling module 34 to ensure that all operations are performed under optimal conditions.
[0028] Please see Figure 1 - Figure 4A conveyor belt 16 is installed in the cavity on the upper surface of the mounting frame 1. A rotating column 18 connected to the mounting frame 1 is located inside the end of the conveyor belt 16 near the cold table 8. A transmission column 19 is located on the outer rear end of the conveyor belt 16. A transmission module 22 electrically connected to the control board 35 is located on the lower surface of the conveyor belt 16. The design of the conveyor belt 16 system, including components such as the rotating column 18 and the transmission column 19, realizes automated sample transfer, improving processing efficiency and ease of operation. A support frame 23 is installed at the lower rear end of the conveyor belt 16. A motor 21 is installed on the upper end of the support frame 23. A transmission belt 20 meshes with the transmission column 19 at the output end of the motor 21. The motor 21 is installed on the support frame 23 and meshes with the transmission column 19 through the transmission belt 20, providing a stable power source for the conveyor belt 16 and ensuring the smoothness and accuracy of the sample transfer process. A second magnetic block 17 corresponding to the third magnetic block 33 is located at the center of the upper surface of the conveyor belt 16. A support pad 2 is fixedly connected to the center of its front end. The corresponding design of the placement plate 14, the second magnetic block 17, and the third magnetic block 33 ensures the precise positioning of the sample on the conveyor belt 16, enhancing the accuracy and reliability of the device operation. The center of the upper surface of the placement plate 14 is provided with a first magnetic block 15 corresponding to the second magnetic block 17. The side surface of the mounting frame 1 is provided with a push-pull plate 9, and the center of the surface of the push-pull plate 9 is provided with a handle 10. The first magnetic block 15 on the placement plate 14 works in conjunction with the second magnetic block 17 to facilitate the quick loading and unloading of samples. The push-pull plate 9 is equipped with a handle 10 for user operation. The upper surface of the mounting frame 1 is provided with a fixing plate 4 on one side of the cold stage 8. The center of the upper surface of the fixing plate 4 is provided with a robotic arm 5 electrically connected to the control board 35. The piston end of the robotic arm 5 clamps and installs a vacuum pump 6. The output end of the vacuum pump 6 is installed with a negative pressure suction head 7. The robotic arm 5, which is electrically connected to the control board 35, is mounted on the fixing plate 4. The vacuum pump 6 it clamps processes the sample through the negative pressure suction head 7, achieving precise and efficient sample collection.
[0029] A drive column 19 is provided on the outer rear end of the conveyor belt 16, while a drive module 22 electrically connected to the control board 35 is mounted on the lower surface of the conveyor belt 16. When samples need to be transferred, the drive module 22 is activated, driving the conveyor belt 16 to rotate. Automated sample transfer is achieved through the coordinated action of components such as the rotating column 18 and the drive column 19. The support frame 23 is located at the lower rear end of the conveyor belt 16, and a motor 21 is mounted on its upper end. The output end of the motor 21 provides a stable power source to the conveyor belt 16 through a drive belt 20 that meshes with the drive column 19. The motor 21 drives the drive belt 20 to rotate, thereby driving the drive column 19 and the entire conveyor belt 16 system to operate, ensuring the stability and accuracy of the samples throughout the transfer process. A second magnetic block 17 is provided at the center of the upper surface of the conveyor belt 16. Corresponding to the third magnetic block 33 under the insulated container 32 of the cold stage 8, the sample is accurately positioned on the conveyor belt 16. The support pad 2 is fixedly connected to the center of the front end of the placement plate 14. The center of the upper surface of the placement plate 14 is provided with a first magnetic block 15, which works in conjunction with the second magnetic block 17 on the conveyor belt 16. This design facilitates the rapid loading and unloading of samples and ensures that the sample can be stably and quickly positioned when entering or leaving the conveyor belt 16. A fixing plate 4 is provided on the upper surface of the mounting frame 1 on one side of the cold stage 8. A robotic arm 5 electrically connected to the control board 35 is installed at the center of the upper surface of the fixing plate 4. A vacuum pump 6 is clamped and installed at the piston end of the robotic arm 5. A negative pressure suction head 7 is installed at the output end of the vacuum pump 6. When it is necessary to process the sample, the robotic arm 5 can be accurately positioned to the designated position.
[0030] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A pleural effusion cell freezing section and fluorescent probe dyeing integrated device, characterized in that, The utility model provides a chest and abdominal cavity liquid cell detection device, including installation frame (1), still including support pad (2), detection frame (11), liquid storage tank (12), access (13), slicing knife (24), electric push rod (25), detection module (26), mounting bracket (27), control valve (28), probe dye output pipe (29), reaction termination liquid output pipe (30) and control panel (35), the outer side of installation frame (1) upper surface is surrounded and is installed support pad (2), and the center of installation frame (1) upper end is equipped with detection frame (11), and the rear end of detection frame (11) inside upper side is installed electric push rod (25) for the slicing of chest and abdominal cavity liquid cell, and the piston end of electric push rod (25) is equipped with slicing knife (24), and the inside center of detection frame (11) and the inside front end are equipped with mounting bracket (27), and the inside center and the front end inside of mounting bracket (27) are equipped with two groups of control valve (28) for controlling flow, and the upper end of control valve (28) is equipped with liquid storage tank (12), and the center of liquid storage tank (12) upper surface is equipped with access (13), and the center of control valve (28) inside center lower surface is equipped with probe dye output pipe (29) for being combined with protein, positioning in sample and visualizing biomolecule, and the center of control valve (28) front end inside lower surface is equipped with reaction termination liquid output pipe (30) for stopping specific biochemical reaction, to prevent the result distortion caused by excessive reaction, and the front end of detection frame (11) side surface is equipped with control panel (35) and is electrically connected with control valve (28), and the inside upper side of detection frame (11) is installed detection module (26) and is electrically connected with control panel (35) before reaction termination liquid output pipe (30), probe dye output pipe (29) and electric push rod (25).
2. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 1, characterized in that, The upper surface of the installation frame (1) is provided with a cold table (8) at the rear end of the detection frame (11), and the inner center of the cold table (8) is provided with a heat preservation container (32), and the inner lower surface of the heat preservation container (32) is provided with a third magnetic block (33).
3. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 2, characterized in that, The side of the cold table (8) close to the detection frame (11) is provided with a sliding groove (31), and the lower center of the cold table (8) is provided with a refrigeration module (34) electrically connected with the control panel (35), and the side surface of the installation frame (1) is provided with a heat dissipation groove (3) corresponding to the refrigeration module (34).
4. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 1, characterized in that, The upper surface of the installation frame (1) is provided with a conveyor belt (16), and the inner end of the conveyor belt (16) close to the cold table (8) is provided with a rotating column (18) connected with the installation frame (1), and the outer end of the conveyor belt (16) is provided with a transmission column (19), and the lower surface of the conveyor belt (16) is provided with a transmission module (22) electrically connected with the control panel (35).
5. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 4, characterized in that, The lower end of the rear side of the conveyor belt (16) is provided with a support frame (23), and the upper end of the support frame (23) is provided with a motor (21), and the output end of the motor (21) is provided with a transmission belt (20) meshed with the transmission column (19).
6. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 4, characterized in that, The upper center of the conveyor belt (16) is provided with a second magnetic block (17) corresponding to the third magnetic block (33), and the front center of the support pad (2) is fixedly connected with a placing plate (14).
7. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 6, characterized in that, The center of the upper surface of the placing plate (14) is provided with a first magnetic attraction block (15) corresponding to the second magnetic attraction block (17), and the side surface of the mounting frame (1) is provided with a push-pull plate (9), and the surface center of the push-pull plate (9) is provided with a handle (10).
8. The pleural effusion cell freezing section and fluorescent probe dyeing integrated device according to claim 1, characterized in that, The upper surface of the mounting frame (1) located on one side of the cooling table (8) is provided with a fixed plate (4), the upper surface center of the fixed plate (4) is provided with a mechanical arm (5) electrically connected with the control panel (35), the piston end of the mechanical arm (5) is clamped and mounted with a vacuum pump (6), and the output end of the vacuum pump (6) is mounted with a negative pressure suction head (7).