Stem cell culture device for rapid detection

Abstract

The utility model discloses a stem cell culture device for rapid detection. The stem cell culture device comprises a culture bin, a partition plate is arranged in the culture bin, a second rotating rod is arranged at the bottom in the partition plate, a rotating disc is arranged at the top of the second rotating rod, a plurality of sets of placing grooves are evenly formed in the outer side of the rotating disc, and culture dishes are arranged in the placing grooves. According to the stem cell culture device, a plurality of cavities can be formed in the culture device under the cooperation of the culture bin and the partition plate, so that the environment can be adjusted according to different stem cells, and then the stem cells can be conveniently cultured in the culture process through the cooperation of the rubber head dropper, the first rotating rod, the placing groove, the rotating disc, the second rotating rod, the inserting rod, the inserting groove, the connecting groove and the spring. A culture solution can be added into the culture dish under the condition that the device is not opened, calibration is not needed in the process of adjusting the position of the culture dish, the interior of the culture dish can be rapidly positioned, and the culture difficulty is reduced.

Description

Technical Field

[0001] This utility model relates to the field of stem cell technology, specifically to a stem cell culture device for rapid detection. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity and can produce at least one type of highly differentiated daughter cells. Stem cell culture is a complex and highly specialized technique that involves the key environment that maintains the self-renewal and multi-lineage differentiation potential of stem cells.

[0003] A stem cell culture device for rapid detection, with existing patent publication number "CN219752329U", includes a cylindrical base with an open top and a closed bottom. An extraction layer and a culture layer are sequentially inserted and connected to the upper part of the base. Both the extraction layer and the culture layer have open tops and are sealed at the bottom by a semi-permeable membrane. The sidewalls of the culture layer have several vent holes, which are sealed by the semi-permeable membrane. A top cover is inserted and connected to the upper part of the culture layer. This invention can perform stem cell culture and pretreatment for detection, meeting the needs of rapid stem cell detection.

[0004] However, this device can only culture a single group of stem cells during the culture process. In contrast, multiple groups of stem cells need to be cultured together during the culture process, and the stem cells need to be replenished quickly in order to conduct experiments. However, this device has low culture efficiency and cannot culture multiple groups of stem cells together. Utility Model Content

[0005] The purpose of this invention is to provide a rapid detection device for stem cell culture, so as to solve the existing problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a stem cell culture device for rapid detection, comprising a culture chamber, an interior partition, a second rotating rod at the bottom of the partition, a turntable at the top of the second rotating rod, a plurality of placement slots evenly distributed on the outer side of the turntable, a culture dish inside each placement slot, a first rotating rod at the top of the turntable, a top cover at the top of the culture chamber, the top of the first rotating rod penetrating the top cover, a connecting tube on the outer side of the first rotating rod connected to the top cover, a plurality of slots evenly distributed on the inner side of the connecting tube, a plurality of connecting slots evenly distributed on the outer side of the first rotating rod, a spring inside each connecting slot, and a insertion rod at each spring.

[0007] The top of the top cover is evenly provided with multiple sets of connecting seats, and the inside of the connecting seats is provided with a dropper. The inside of the culture chamber is evenly provided with multiple sets of timer switches on both sides. The bottom of the top cover is evenly provided with multiple sets of sealing plugs, and the outside of the sealing plugs is provided with grooves. The inside of the culture chamber is provided with multiple sets of ultraviolet lamps in the middle of both sides.

[0008] Preferably, the top of the connector is provided with a sealing groove, and the bottom of the dropper is located inside the sealing groove, which facilitates the replacement of the dropper.

[0009] Preferably, the culture chamber has symmetrical mounting grooves at both ends, and the inside of the mounting grooves is equipped with a light-transmitting plate to facilitate observation of the inside of the culture chamber.

[0010] Preferably, the culture chamber is provided with handles on both sides symmetrically to facilitate moving the culture chamber to change its position.

[0011] Preferably, the top of the turntable is provided with an insertion tube, and the two ends of the insertion tube are symmetrically provided with limiting grooves. The bottom of both ends of the first rotating rod are symmetrically provided with limiting rods, and the limiting rods and limiting grooves are adapted to each other, so that the turntable can be driven to rotate during the rotation of the first rotating rod.

[0012] Preferably, the bottom of the outer side of the groove is provided with a sloping guide plate to facilitate pressing the timer switch.

[0013] Preferably, the bottom of the culture chamber is provided with a connecting bearing, and the second rotating rod is located inside the bearing to reduce friction during rotation.

[0014] Preferably, the top of the first rotating rod is provided with a rotating plate to reduce the difficulty of rotation.

[0015] Compared with the prior art, the beneficial effects of this utility model are: the stem cell culture device for rapid detection;

[0016] The combination of culture chambers and partitions creates multiple cavities within the culture device, allowing for environmental adjustments based on the specific stem cell type. A combination of a dropper, first rotating rod, placement slot, turntable, second rotating rod, insertion rod, slot, connecting slot, and springs enables the addition of culture medium to the culture dish without opening the device. Furthermore, no calibration is required when adjusting the dish's position, allowing for rapid positioning and reducing the complexity of the culture process. Finally, a combination of ultraviolet light, sealing plug, and timer switch ensures the device's interior is sterilized before culture, preventing environmental contamination. Attached Figure Description

[0017] Figure 1 This is a perspective view of the present utility model;

[0018] Figure 2 This is a front sectional view of the present invention;

[0019] Figure 3 This is a top view of the present invention;

[0020] Figure 4 This is a front sectional view of the mounting frame of this utility model;

[0021] Figure 5 This is a top sectional view of the connecting pipe of this utility model.

[0022] In the diagram: 1. Culture chamber; 2. Ultraviolet lamp; 3. Top cover; 4. Sealing groove; 5. Connecting seat; 6. Dropper; 7. First rotating rod; 8. Connecting tube; 9. Sealing plug; 10. Placement groove; 11. Turntable; 12. Second rotating rod; 13. Partition; 14. Insert rod; 15. Slot; 16. Connecting groove; 17. Spring; 18. Timer switch; 19. Groove; 20. Petri dish. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-5The present invention provides an embodiment of a stem cell culture device for rapid detection, comprising a culture chamber 1. The culture chamber 1 has symmetrical handles on both sides for easy movement and repositioning. The culture chamber 1 has symmetrical mounting slots at both ends, with a light-transmitting plate inside each slot for easy observation of the interior. The culture chamber 1 has a partition 13 inside, with a second rotating rod 12 at the bottom of the partition 13. A connecting bearing is located at the bottom of the culture chamber 1, and the second rotating rod 12 is located inside the bearing to reduce friction during rotation. A turntable 11 is located at the top of the second rotating rod 12. Multiple sets of placement slots 10 are evenly distributed on the outer side of the turntable 11, with a culture dish 20 placed inside each slot. The top of the culture chamber 1 is provided with a first rotating rod 7, and the top of the first rotating rod 7 is provided with a rotating plate to reduce the difficulty of rotation. The top of the turntable 11 is provided with an insertion tube, and the two ends of the insertion tube are symmetrically provided with limiting grooves. The bottom of both ends of the first rotating rod 7 are symmetrically provided with limiting rods, and the limiting rods and limiting grooves are adapted to each other. During the rotation of the first rotating rod 7, the turntable 11 can be driven to rotate. The top of the culture chamber 1 is provided with a top cover 3, and the top of the first rotating rod 7 passes through the top cover 3. The outside of the first rotating rod 7 is provided with a connecting tube 8, which is connected to the top cover 3. The inside of the connecting tube 8 is evenly provided with multiple sets of slots 15, and the outside of the first rotating rod 7 is evenly provided with multiple sets of connecting grooves 16. The inside of the connecting groove 16 is provided with a spring 17, and the spring 17 is provided with an insertion rod 14.

[0025] Multiple sets of connecting seats 5 are evenly arranged on the top of the top cover 3. A dropper 6 is provided inside the connecting seat 5. A sealing groove 4 is provided on the top of the connecting seat 5. The bottom of the dropper 6 is located inside the sealing groove 4, which facilitates the replacement of the dropper 6. Multiple sets of timer switches 18 are evenly arranged on both sides inside the culture chamber 1. Multiple sets of sealing plugs 9 are evenly arranged on the bottom of the top cover 3. A groove 19 is provided on the outside of the sealing plug 9. A sloping guide plate is provided at the bottom of the outside of the groove 19, which facilitates pressing the timer switch 18. Multiple sets of ultraviolet lamps 2 are provided in the middle of both sides inside the culture chamber 1.

[0026] Working principle: Before cultivation, first turn on the power, then put on the top cover 3. During the process of putting on the top cover 3, the groove 19 squeezes the timer switch 18, then turn on the ultraviolet lamp 2, and then use the ultraviolet lamp 2 to sterilize the inside of the cultivation chamber 1. After sterilization for 30 minutes, the ultraviolet lamp 2 is turned off. At this time, the power is disconnected and the top cover 3 is opened. At the same time, alcohol is sprayed into the inside of the cultivation chamber 1, and the culture dish 20 is placed inside the placement tank 10. Then the sealing plug 9 is put on.

[0027] During the process of sealing the plug 9, the first rotating rod 7 is also inserted into the turntable 11 and connected to the turntable 11. Then, the stem cells are cultured inside the culture chamber 1. After the culture time is up, the culture medium is added to the culture dish 20 by squeezing the dropper 6. After the culture medium is added to a culture dish 20, the first rotating rod 7 is rotated. The first rotating rod 7 drives the turntable 11 and the placement groove 10 to rotate. At the same time, the insertion rod 14 is squeezed and disengaged from the slot 15, and drives the spring 17 to compress. When it moves to the next culture dish 20, the spring 17 returns to its original position and pushes the insertion rod 14 into the next slot 15, thus completing the switching of the culture dish 20. Then, the culture medium is added to the culture dish 20.

[0028] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0029] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0030] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection or a detachable connection; a mechanical connection or an electrical connection; a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

Claims

1. A rapid detection stem cell culture device comprising a culture chamber (1), characterized in that: The inside of the culture bin (1) is provided with a partition (13), the bottom of the inside of the partition (13) is provided with a second rotating rod (12), the top of the second rotating rod (12) is provided with a rotating disc (11), the outside of the rotating disc (11) is uniformly provided with a plurality of groups of placing grooves (10), the inside of the placing groove (10) is provided with a culture dish (20), the top of the rotating disc (11) is provided with a first rotating rod (7), the top of the culture bin (1) is provided with a top cover (3), the top of the first rotating rod (7) penetrates the top cover (3), the outside of the first rotating rod (7) is provided with a connecting pipe (8), the connecting pipe (8) is connected with the top cover (3), the inside of the connecting pipe (8) is uniformly provided with a plurality of groups of insertion grooves (15), the outside of the first rotating rod (7) is uniformly provided with a plurality of groups of connecting grooves (16), the inside of the connecting groove (16) is provided with a spring (17), the spring (17) is provided with an insertion rod (14); The top of the top cover (3) is uniformly provided with a plurality of groups of connecting seats (5), the inside of the connecting seat (5) is provided with a rubber head dropper (6), the inside of the culture bin (1) is uniformly provided with a plurality of groups of timing switches (18) on both sides, the bottom of the top cover (3) is uniformly provided with a plurality of groups of sealing plugs (9), the outside of the sealing plug (9) is provided with a groove (19), the inside of the culture bin (1) is provided with a plurality of groups of ultraviolet lamps (2) in the middle of both sides.

2. The stem cell culture device for rapid detection according to claim 1, characterized in that: The top of the connecting seat (5) is provided with a sealing groove (4), and the bottom of the rubber head dropper (6) is located in the inside of the sealing groove (4).

3. The stem cell culture device for rapid detection according to claim 1, characterized in that: Both ends of the culture bin (1) are symmetrically provided with mounting grooves, and the inside of the mounting grooves is provided with light transmission plates.

4. The stem cell culture device for rapid detection according to claim 1, characterized in that: Both sides of the culture bin (1) are symmetrically provided with handles.

5. The stem cell culture device for rapid detection according to claim 1, characterized in that: The top of the rotating disc (11) is provided with an insertion pipe, and both ends of the inside of the insertion pipe are symmetrically provided with limiting grooves, the bottoms of both ends of the first rotating rod (7) are symmetrically provided with limiting rods, and the limiting rods and the limiting grooves are mutually matched.

6. The stem cell culture device for rapid detection according to claim 1, wherein: The bottom of the outside of the groove (19) is provided with an inclined guide plate.

7. The stem cell culture device for rapid detection according to claim 1, characterized in that: The bottom of the inside of the culture bin (1) is provided with a connecting bearing, and the second rotating rod (12) is located in the inside of the bearing.

8. The stem cell culture device for rapid detection according to claim 1, characterized in that: The top of the first rotating rod (7) is provided with a rotating plate.

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