Soil organic carbon mineralization incubation bottle

Abstract

The utility model discloses a soil organic carbon mineralization culture bottle of soil ecological research equipment technical field, including bottle body, the surface middle part of bottle body is equipped with the rotary seal of limit of carousel, the top of bottle body is equipped with the connecting end, the top annular of connecting end is equipped with the threaded column, the top surface embedding of threaded column is equipped with the sealing ring, the top of connecting end is equipped with the sealing cover, the bottom between sealing cover and threaded column is the screw rotation connection, the inside middle part of bottle body is equipped with the container, the outside surface of container is equipped with the sleeve piece of limit of rotary sleeve, through the radial rotation of multiple groups of lye absorption cup that set up in the inside annular of bottle body, the target rotation is adjusted to the just below of sampling port, and the sampling ware tip is inserted to lye absorption cup from the surface middle part of silica gel valve and carries out sampling, and the needle -pricking silica gel valve that sets up can be healed after the sampling ware is pulled out, effectively blocks the entry of outside mixed impurity oxygen, and does not need to open the sealing cover.

Description

Technical Field

[0001] This utility model belongs to the technical field of soil ecological research equipment, specifically relating to a soil organic carbon mineralization culture bottle. Background Technology

[0002] Soil carbon includes inorganic carbon and organic carbon. Soil organic carbon is the general term for all carbon-containing organic matter in the soil, including plant and animal remains, microbial metabolites, etc. It is not only closely related to soil fertility, but also has a huge impact on the Earth's carbon cycle. The mineralization of soil organic carbon affects not only soil fertility and the carbon cycle of ecosystems, but is also closely related to climate change. Traditional soil organic carbon mineralization incubation experiments involve placing a certain mass of treated soil sample into a culture container, spreading the soil evenly at the bottom of the container, and then placing the culture container in a constant temperature incubator. During the incubation process, the absorbent absorbs carbon dioxide released by soil organisms. Subsequently, a standard hydrochloric acid solution is used for titration, and the volume of hydrochloric acid consumed characterizes the rate of soil organic carbon mineralization.

[0003] Existing soil organic carbon mineralization culture bottles require opening for sampling during use, which can easily introduce oxygen containing mixed impurities from the outside into the bottle, thereby altering the anaerobic metabolic pathway, damaging the internal environment, causing contamination during operation, and causing inconvenience to subsequent work. To address this, we propose a new soil organic carbon mineralization culture bottle. Utility Model Content

[0004] The purpose of this invention is to provide a soil organic carbon mineralization culture bottle to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a soil organic carbon mineralization culture bottle, comprising a bottle body, a turntable rotatably and sealingly installed at the center of the bottle body surface, a connecting end at the top of the bottle body, a threaded post annularly installed at the top of the connecting end, a sealing ring embedded in the top surface of the threaded post, a sealing cap above the connecting end, the bottom of the sealing cap being threadedly and rotatably connected to the threaded post, a container installed at the center of the inner side of the bottle body, a sleeve block rotatably and limitingly fitted on the outer surface of the container, multiple sets of connecting rods fixedly and annularly connected to the outer surface of the sleeve block, the ends of the connecting rods being fixedly connected to the inner wall of the turntable, an alkali absorption cup fixedly installed above the ends of the connecting rods, a sampling port penetrating one side of the surface of the sealing cap, and a needle-pierced silicone valve embedded and sealed inside the sampling port.

[0006] Preferably, an observation window is provided in the center of the surface of the sealing cover, and the membrane inside the observation window is composed of a PTFE membrane.

[0007] Preferably, a first probe interface is installed on one side of the surface of the sealing cover.

[0008] Preferably, a second probe interface is installed on one side of the surface of the sealing cover.

[0009] Preferably, the outer surface of the turntable is provided with annular anti-slip texture.

[0010] Preferably, handles are symmetrically fixedly installed on both outer surfaces of the bottle.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. Multiple sets of alkaline absorption cups are arranged in a circular radial rotation inside the bottle. The target is rotated and adjusted to be directly below the sampling port. The sampler tip is inserted into the alkaline absorption cup from the middle of the surface of the silicone valve to take a sample. The needle-pierced silicone valve can automatically heal after the sampler is pulled out, effectively blocking the entry of oxygen mixed with external impurities, and there is no need to open the sealing cap.

[0013] 2. The first and second probe interfaces facilitate the connection of oxygen and carbon dioxide sensors, enabling real-time monitoring of oxygen and carbon dioxide levels inside the bottle. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the sealing cap and bottle structure of this utility model;

[0015] Figure 2 This is a top view of the internal structure of the bottle body of this utility model;

[0016] Figure 3 This is a schematic diagram of the upper and lower connection structure of the turntable of this utility model;

[0017] Figure 4 This is a front structural diagram of the present invention.

[0018] In the diagram: 1. Bottle body; 2. Turntable; 3. Anti-slip texture; 4. Connecting end; 5. Threaded post; 501. Sealing ring; 6. Sealing cap; 7. Observation window; 8. Sampling port; 9. Silicone valve; 10. First probe interface; 11. Second probe interface; 12. Container; 13. Sleeve block; 14. Connecting rod; 15. Alkali absorption cup; 16. Handle. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figure 1-4 This utility model provides a technical solution: a soil organic carbon mineralization culture bottle, including a bottle body 1, a turntable 2 is installed in the middle of the surface of the bottle body 1 for limiting rotation and sealing, a connecting end 4 is provided at the top of the bottle body 1, a threaded post 5 is installed in a ring on the top of the connecting end 4, a sealing ring 501 is embedded in the top surface of the threaded post 5, a sealing cover 6 is provided above the connecting end 4, the bottom of the sealing cover 6 is threadedly rotatably connected to the threaded post 5, a container 12 is installed in the middle of the inner side of the bottle body 1, a sleeve block 13 is fitted in a limiting rotation on the outer surface of the container 12, a plurality of connecting rods 14 are fixedly connected in a ring on the outer surface of the sleeve block 13, the end of the connecting rod 14 is fixedly connected to the inner wall of the turntable 2, an alkaline absorption cup 15 is fixedly installed above the end of the connecting rod 14, a sampling port 8 is opened through one side of the surface of the sealing cover 6, and a needle-punched silicone valve 9 is embedded and sealed inside the sampling port 8.

[0021] Specifically, an observation window 7 is provided in the middle of the surface of the sealing cover 6, and the membrane inside the observation window 7 is composed of a PTFE membrane.

[0022] Specifically, a first probe interface 10 is installed on one side of the surface of the sealing cover 6.

[0023] Specifically, a second probe interface 11 is installed on one side of the surface of the sealing cover 6.

[0024] Specifically, the outer surface of the turntable 2 is provided with an anti-slip texture 3 in a ring shape. The anti-slip texture 3 increases the friction between the user and the turntable 2, making it easier to use.

[0025] Specifically, handles 16 are symmetrically fixedly installed on both outer surfaces of the bottle body 1, which facilitates the movement and use of the bottle body 1.

[0026] In this embodiment, the sealing cap 6 is separated from the connecting end 4. The pretreated soil sample is placed into the container 12 in the bottle body 1, and an appropriate amount of water is added to adjust it to 60% of the maximum water holding capacity. Multiple sets of alkaline absorption cups 15 are used to fill the CO2 absorption solution. Then, the sealing cap 6 and the threaded post 5 above the connecting end 4 are connected by a threaded rotation. The sealing ring 501 increases the sealing effect between the sealing cap 6 and the bottle body 1. Finally, the bottle body 1 is placed under constant temperature conditions for cultivation. When it is necessary to take a sample from the inside of the bottle body 1, the turntable 2 drives the connecting rod 14 to rotate. The connecting rod 14 rotates the target alkaline absorption cup 15 to the direct below the sampling port 8. Then, the sampler tip is inserted from the middle of the surface of the silicone valve 9 into the alkaline absorption cup 15 for aspiration. Finally, the sample is titrated and quantitatively calculated to obtain the experimental results. The set needle-pierced silicone valve 9 can automatically heal after the sampler is pulled out, effectively blocking the entry of oxygen from external mixed impurities.

[0027] Through the first probe interface 10 and the second probe interface 11, the required gas can be connected and input into the bottle as needed. After the input is completed, the oxygen sensor and carbon dioxide sensor can be connected as needed to achieve real-time monitoring of oxygen and carbon dioxide inside the bottle 1.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A soil organic carbon mineralization culture bottle, comprising a bottle body (1), characterized in that: A turntable (2) is installed in the middle of the surface of the bottle body (1) for limiting rotation and sealing. A connecting end (4) is provided at the top of the bottle body (1). A threaded column (5) is installed in a ring at the top of the connecting end (4). A sealing ring (501) is embedded in the top surface of the threaded column (5). A sealing cover (6) is provided above the connecting end (4). The bottom of the sealing cover (6) is connected to the threaded column (5) by a threaded rotation. A container (12) is installed in the middle of the inner side of the bottle body (1). A sleeve block (13) is fitted in a limiting rotation on the outer surface of the container (12). Multiple sets of connecting rods (14) are fixedly connected in a ring on the outer surface of the sleeve block (13). The end of the connecting rod (14) is fixedly connected to the inner wall of the turntable (2). An alkaline absorption cup (15) is fixedly installed above the end of the connecting rod (14). A sampling port (8) is opened through one side of the surface of the sealing cover (6). A needle-punched silicone valve (9) is embedded in the inner side of the sampling port (8) for sealing.

2. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: An observation window (7) is provided in the middle of the surface of the sealing cover (6), and the membrane inside the observation window (7) is composed of a PTFE membrane.

3. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: The first probe interface (10) is installed on one side of the surface of the sealing cover (6).

4. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: A second probe interface (11) is installed on one side of the surface of the sealing cover (6).

5. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: The outer surface of the turntable (2) is provided with anti-slip texture (3).

6. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: Handles (16) are symmetrically fixed on both sides of the outer surface of the bottle body (1).

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