Methods and compositions related to recombinant neil2
a technology of recombinant neil and composition, applied in the field of recombinant neil2, can solve the problems of affecting the ability of the host, causing genome damage due to oxygen free radicals, and accumulation of damage in the transcribed sequence can be lethal to the host cell, so as to increase the ros-generation, prevent the effect of mortality, and effective therapeutic intervention in bacterial infection
Patent Information
- Authority / Receiving Office
- US · United States
- Patent Type
- Applications(United States)
- Current Assignee / Owner
- Publication Date
- 2020-04-02
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application claims priority to U.S. Provisional Application No. 62 / 738,605 filed Sep. 28, 2019, which is incorporated by reference herein in its entirety.STATEMENT REGARDING FEDERALLY FUNDED RESEARCH
[0002] This invention was made with government support under NS073976 awarded by the National Institute of Neurological Disorders and Stroke (NINDS). The government has certain rights in the invention.BACKGROUND OF THE INVENTIONI. Field of the Invention
[0003] The embodiments described herein generally concern the field of medicine and pathological inflammation. Particular embodiments are directed to compositions and methods for administering rNEIL2 to a subject in need.II. Description of Related Art
[0004] Allergic asthma (AA) and allergic rhinitis (AR) are highly prevalent diseases of the airways, which are characterized by the presence of mucosal inflammation, reversible obstruction and airway hyperreactivity. These diseases have a s...
Examples
Embodiment Construction
[0043]Ragweed pollen (RWP) and cat dander are major allergens associated with AA and AR. The innate pathways that provide protection against innate (IIR) and allergic (AIR) immune / inflammatory airway responses induced by these and other allergens are of great scientific interest. The inventors made a surprising observation that Neil-like 2 (NEIL2), which the inventors cloned and characterized, stably associates with the cytosolic DNA sensor Stimulator of Interferon Genes complex (STING) only in allergic mice and human subjects, and intrapulmonary administration of recombinant (r)NEIL2 markedly decreases cat dander extract (CDE)-induced AIR. The central hypothesis is that NEIL2 protects against allergen-induced gene transcription, IIR and AIR in sensitized mice and humans by: (i) binding to the promoter region of specific genes in the nucleus and (ii) complexing with STING in the cytosol. Binding of NEIL2, STAT6- and NFkB to the promoter regions of genes and the type of oxidatively d...