The invention provides a biological environment-friendly spraying emulsion for branches and trunks of fruit trees. The spraying emulsion is prepared by the following steps: crushing attapulgite, diatomite and a biomass material into 100-to-200-mesh fine powder, and then adding the fine powder, water, amino acid, a compound microbial agent, a chelating liquid nitrogen fertilizer, potassium phosphite and mineral-derived potassium fulvate into a colloid mill for grinding, adding the ground materials, Vitacat, orange peel essential oil and organic silicon into a homogenizer, and carrying out homogenizing and dispersing to obtain the product. The orange peel essential oil, the organic silicon and a polyacrylamide water-retaining agent are selected as coupling agents, and secondary grinding and dispersion are carried out through the colloid mill and the homogenizer, so a part of ions can reach a nanoscale level, and the adhesiveness, the permeability, the moisture retention and the anti-freezing performance of the product are greatly improved; and according to the invention, the biomass material and the compound microbial agent are employed and can prevent and treat diseases, prevent insects and expel insects, and the chelating liquid nitrogen fertilizer, the potassium phosphite, the mineral-derived potassium fulvic acid and the Vitacat are selected and used, so nutrients can be efficiently supplied to fruit trees through branches and trunks.