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Epitope capable of exciting human body anti-mycobacterium tuberculosis protective immunity reaction and use thereof

A technology of protective immunity and antigenic epitopes, applied in the field of molecular immunology, can solve the problems of serious diseases and even death, loss of diagnostic significance of Mycobacterium tuberculosis infection, inability to escape immune recognition, etc., to promote the resolution and promotion of multi-drug resistance puzzle effect

Inactive Publication Date: 2011-01-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, multiple antibiotics need to be used in combination for at least 6 months, and due to the widespread emergence of Mtb drug-resistant strains, antibiotic treatment is often ineffective
The tuberculosis vaccine commonly used today, Bacillus Calmette-Guerin (BCG), has significant limitations: (1) Its effect on adult tuberculosis is not satisfactory; (2) It can cause severe disease and even death in immunocompromised individuals (3) It can cause positive skin test, making it lose its diagnostic significance in Mycobacterium tuberculosis infection
While vaccine research has focused on immunodominant epitopes, studies of minor determinants may focus the immune response on epitopes that cannot escape immune recognition
It has been reported that immunization of mice with a minor determinant of ESAT-6 can induce a significant protective immune response against TB, while some immunodominant epitopes cannot produce such a protective response

Method used

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  • Epitope capable of exciting human body anti-mycobacterium tuberculosis protective immunity reaction and use thereof
  • Epitope capable of exciting human body anti-mycobacterium tuberculosis protective immunity reaction and use thereof
  • Epitope capable of exciting human body anti-mycobacterium tuberculosis protective immunity reaction and use thereof

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Experimental program
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Embodiment 1

[0030] 1Materials and methods

[0031] 1.1 Research object

[0032] There were 200 confirmed pulmonary tuberculosis patients, of which 100 were HLA-A*0201 positive and 100 were HLA-A*0201 negative, all of which were examined by radiology and clinical sputum culture. 100 HLA-A*0201 positive patients with other chronic lung diseases were the disease control group, and 100 HLA-A*0201 positive healthy volunteers were the normal control group.

[0033] 1.2 Reagents and kits:

[0034] Lymphocyte separation solution LymphoprepTM was purchased from AXIS-SHIELD, Norway;

[0035] LPS, human recombinant IL-2, DMSO, BFA, β 2-mg were all purchased from Sigma;

[0036] Human recombinant GM-CSF and human recombinant IL-4 were purchased from R&D company;

[0037] RPMI1640 medium and fetal bovine serum were purchased from Gibco; (Sigma);

[0038] FITC-labeled anti-CD3, anti-CD8, anti-HLA.A2 flow cytometric monoclonal antibodies and FITC-labeled antibodies for detecting intracellular factors (IFN-γ, TNF-Q,...

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Abstract

The invention relates to the field of molecular immunology. In recent years, tuberculosis begins to resume throughout the world and antibiotic treatment often fails because of the appearance of drug-resistant strain; the tuberculosis vaccines, namely, bacillus calmette-guerin vaccines, which are commonly used, are also limited. The purpose of the invention is to screen out the antigen epitope molecular simulating peptide which can stimulate the protective immune reaction of anti-mycobacterium tuberculosis in human bodies, study the protective immune reaction mechanism and further develop novel concatenated polyepitope tuberculosis vaccines. The invention provides an antigen epitope molecular simulating peptide which can stimulate the protective immune reaction of anti-mycobacterium tuberculosis in human bodies, the amino acid sequence of the peptide is shown in SEQ ID NO: 10. The invention also provides a screening method and usage of the peptide. The invention prevents and controls the occurrence and development of tuberculosis more effectively.

Description

[0001] This application is a divisional application of the patent application with the application number 200610027311.2 filed on June 6, 2006, and the invention title is "Antigenic epitopes that stimulate the human body's protective immune response against Mycobacterium tuberculosis and their use". Technical field: [0002] The present invention relates to the field of molecular immunology, in particular to molecular mimic peptides capable of stimulating the human body's protective immune response against tuberculosis bacilli and their screening methods and uses. Background technique: [0003] In recent years, tuberculosis (Tuberculosis, TB) has revived on a global scale. According to statistics from the World Health Organization, about one-third of the world's population and about 2 billion people are infected with Mycobacterium tuberculosis (Mtb), with about 9 million new cases each year, and up to 3 million deaths each year. The World Health Organization proposed a "global eme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K39/04A61P31/06A61P37/04
Inventor 邓安梅仲人前娄加陶吴传勇陈孙孝周晔陈燕陈波钱蒋廷旺
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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