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Antigen epitope for exciting human anti-tubercle bacillus protective immunoreaction and its use

A technology of protective immunity and antigenic epitope, applied in the field of molecular immunology, can solve the problems of serious disease and even death, loss of diagnostic significance of Mycobacterium tuberculosis infection, ineffectiveness of antibiotic treatment, etc., to promote the solution and promotion of multi-drug resistance problems Effect

Inactive Publication Date: 2008-09-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Application Information

AI Technical Summary

Problems solved by technology

However, multiple antibiotics need to be used in combination for at least 6 months, and due to the widespread emergence of Mtb drug-resistant strains, antibiotic treatment is often ineffective
The tuberculosis vaccine commonly used today, Bacillus Calmette-Guerin (BCG), has significant limitations: (1) Its effect on adult tuberculosis is not satisfactory; (2) It can cause severe disease and even death in immunocompromised individuals (3) It can cause positive skin test, making it lose its diagnostic significance in Mycobacterium tuberculosis infection
While vaccine research has focused on immunodominant epitopes, studies of minor determinants may focus the immune response on epitopes that cannot escape immune recognition
It has been reported that immunization of mice with a minor determinant of ESAT-6 can induce a significant protective immune response against TB, while some immunodominant epitopes cannot produce such a protective response

Method used

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  • Antigen epitope for exciting human anti-tubercle bacillus protective immunoreaction and its use
  • Antigen epitope for exciting human anti-tubercle bacillus protective immunoreaction and its use
  • Antigen epitope for exciting human anti-tubercle bacillus protective immunoreaction and its use

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Experimental program
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Effect test

Embodiment 1

[0029] 1 Materials and methods

[0030] 1.1 Research object

[0031] 200 cases of pulmonary tuberculosis were diagnosed, of which 100 were HLA-A*0201 positive and 100 were HLA-A*0201 negative, all of which were tested by radiology and clinical sputum culture. 100 HLA-A*0201 positive patients with other chronic lung diseases were the disease control group, and 100 HLA-A*0201 positive healthy volunteers were the normal control group.

[0032] 1.2 Reagents and kits:

[0033] Lymphoprep TM Purchased from Norway AXIS-SHIELD company;

[0034] LPS, human recombinant IL-2, DMSO, BFA, and β2-mg were all purchased from Sigma;

[0035] Human recombinant GM-CSF and human recombinant IL-4 were purchased from R&D Company;

[0036] RPMI1640 medium and fetal bovine serum were purchased from Gibco; (Sigma);

[0037] FITC-labeled anti-CD3, anti-CD8, anti-HLA.A2 flow cytometric monoclonal antibodies and FITC-labeled antibodies for detecting intracellular factors (IFN-γ, TNF-α, IL-10) are a...

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Abstract

The present invention relates to molecular immunology technology, and aims at screening out antigen epitope molecular simulation peptide capable of exciting human body's protective immunoreaction against tubercle bacillus, researching protective immunoreaction mechanism against tuberculosis, and further developing new type of concatenate polyepitope tuberculosis vaccine. The present invention provides one kind of antigen epitope molecular simulation peptide capable of exciting human body's protective immunoreaction against tubercle bacillus, and the peptide contains the amino acid sequence selected from SEQ ID Nos. 2, 5, 10, 12, 14 and 15. The present invention also provides the screening process and use of the peptide. The present invention may be used in preventing and controlling tuberculosis.

Description

Technical field: [0001] The invention relates to the field of molecular immunology, in particular to a molecular mimetic peptide capable of stimulating a human body's protective immune response against Mycobacterium tuberculosis and its screening method and application. Background technique: [0002] In recent years, tuberculosis (Tuberculosis, TB) has resurged worldwide. According to the statistics of the World Health Organization, about 1 / 3 of the world's population and about 2 billion people are infected with Mycobacterium tuberculosis (Mtb). There are about 9 million new cases every year, and the annual death toll is as high as 3 million. The World Health Organization has proposed a "global tuberculosis emergency" (global emergency). It can be seen that the impact and damage caused by tuberculosis on human health and economy is difficult to simply assess. The characteristics of tuberculosis epidemic in my country are: high prevalence rate, high drug resistance rate, hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K39/04A61P31/06
Inventor 邓安梅仲人前娄加陶吴传勇陈孙孝周晔陈燕陈波钱琤蒋廷旺
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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