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Antigen epitope for exciting protective immunity against tubercle bacillus of human body and uses thereof

A technology of protective immunity and antigenic epitope, applied in the field of molecular immunology, can solve the problems of serious disease and even death, loss of diagnostic significance of Mycobacterium tuberculosis infection, inability to produce such a protective response, etc. Effects of the drug resistance puzzle

Inactive Publication Date: 2008-11-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, multiple antibiotics need to be used in combination for at least 6 months, and due to the widespread emergence of Mtb drug-resistant strains, antibiotic treatment is often ineffective
The tuberculosis vaccine commonly used today, Bacillus Calmette-Guerin (BCG), has significant limitations: (1) Its effect on adult tuberculosis is not satisfactory; (2) It can cause severe disease and even death in immunocompromised individuals (3) It can cause positive skin test, making it lose its diagnostic significance in Mycobacterium tuberculosis infection
While vaccine research has focused on immunodominant epitopes, studies of minor determinants may focus the immune response on epitopes that cannot escape immune recognition
It has been reported that immunization of mice with a minor determinant of ESAT-6 can induce a significant protective immune response against TB, while some immunodominant epitopes cannot produce such a protective response

Method used

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  • Antigen epitope for exciting protective immunity against tubercle bacillus of human body and uses thereof
  • Antigen epitope for exciting protective immunity against tubercle bacillus of human body and uses thereof
  • Antigen epitope for exciting protective immunity against tubercle bacillus of human body and uses thereof

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Experimental program
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Embodiment 1

[0030] 1 Materials and methods

[0031] 1.1 Research object

[0032] 200 cases of pulmonary tuberculosis were diagnosed, of which 100 were HLA-A*0201 positive and 100 were HLA-A*0201 negative, all of which were tested by radiology and clinical sputum culture. 100 HLA-A*0201 positive patients with other chronic lung diseases were the disease control group, and 100 HLA-A*0201 positive healthy volunteers were the normal control group.

[0033] 1.2 Reagents and kits:

[0034] Lymphoprep TM Purchased from Norway AXIS-SHIELD company;

[0035] LPS, human recombinant IL-2, DMSO, BFA, β 2 -mg were all purchased from Sigma Company;

[0036] Human recombinant GM-CSF and human recombinant IL-4 were purchased from R&D Company;

[0037] RPMI1640 medium and fetal bovine serum were purchased from Gibco; (Sigma);

[0038] FITC-labeled anti-CD3, anti-CD8, anti-HLA.A2 flow cytometric monoclonal antibodies and FITC-labeled antibodies for detecting intracellular factors (IFN-γ, TNF-α, IL-10...

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Abstract

The invention relates to the field of molecular immunology. In recent years, tuberculosis revives in the global range. The emergence of drug-resistant strain always invalidates antibiotic therapy and the normally used tuberculosis vaccine-BCG vaccine also has limitations. The invention aims at screening the molecular simulation peptide of epitope which can activate the protective immune response of human anti-mycobacterium tuberculosis, studying the protective immune response mechanism of the tuberculosis and further developing a novel concatenated poly epitope tuberculosis vaccine. The invention provides the molecular simulation peptide of the epitope which can activate the protective immune response of human anti-mycobacterium tuberculosis; the amino acid sequence of the peptide is shown as SEQ ID NO:14. The invention further provides a screening method and the function of the peptide. Therefore, the molecular simulation peptide of the invention can more effectively prevent and control the generation and the development of the tuberculosis.

Description

[0001] This application is a divisional application of the patent application filed on June 6, 2006 with the application number 200610027311.2 and the title of the invention is "antigen epitope for stimulating human body's protective immune response against Mycobacterium tuberculosis and its use". Technical field: [0002] The invention relates to the field of molecular immunology, in particular to a molecular mimetic peptide capable of stimulating a human body's protective immune response against Mycobacterium tuberculosis and its screening method and application. Background technique: [0003] In recent years, tuberculosis (Tuberculosis, TB) has resurged worldwide. According to the statistics of the World Health Organization, about 1 / 3 of the world's population and about 2 billion people are infected with Mycobacterium tuberculosis (Mtb). There are about 9 million new cases every year, and the annual death toll is as high as 3 million. The World Health Organization has pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K39/04A61P31/06A61P37/04
Inventor 邓安梅仲人前娄加陶吴传勇陈孙孝周晔陈燕陈波钱蒋廷旺
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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