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Chryseobacterium rhizosphaerae and application thereof

A technology of Chryseobacterium aureus and rhizosphere, applied in the field of microorganisms, can solve the problem of low activity

Active Publication Date: 2012-07-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on the research on bacterial cellulase in recent years, but it is generally believed that the activity of bacterial cellulase is lower than that of fungal cellulase

Method used

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  • Chryseobacterium rhizosphaerae and application thereof
  • Chryseobacterium rhizosphaerae and application thereof
  • Chryseobacterium rhizosphaerae and application thereof

Examples

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Embodiment 1

[0023] 1. Isolation of Chryseobacterium rhizosphaerae GIMN 1.005.

[0024] From Vietnam rhizosphere 0-30cm soil sample, take 10g of soil and put it into a triangular flask (with glass beads) filled with 90mL sterile water, shake it at 180rpm for 30 minutes, let it stand for 10 minutes, take the upper layer and dilute it 10 times Afterwards, 0.1 mL was evenly spread on the NA agar medium plate, and the plate was turned over and placed in an incubator at 30° C. for 7 days to obtain the Chryseobacterium rhizosphaerae GIMN1.005 of the present invention.

[0025] The formula of the nutrient agar medium is: 10.0g of peptone, 5g of yeast extract, 5g of sodium chloride, 20.0g of agar, 1000ml of water; pH 6.8-7.0.

[0026] Rhizobacterium rhizobacterium (Chryseobacterium rhizosphaerae) GIMN1.005 of the present invention has the following characteristics:

[0027] 1. Morphological characteristics

[0028] The morphology of Chryseobacterium rhizosphere under the electron microscope was ...

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Abstract

The present invention discloses a Chryseobacterium rhizosphaerae and application thereof. A new bacterium capable of producing cellulase, namely Chryseobacterium rhizosphaerae GIMN1.005 collected in China Center for Type Culture Collection (CCTCC) on Oct 20th, 2009 (address: Wuhan University, Wuhan City, China, and collection number: CCTCC NO: M209230) is obtained by separation. Because the Chryseobacterium rhizosphaerae, which is capable of producing cellulase, can be used in production of cellulase, and has an important value in production of cellulase, research on cellulose degradation by utilizing cellulase, and application thereof.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, in particular to a new bacterium capable of producing cellulase-chryseobacterium rhizosphere and its application. Background technique: [0002] Since the discovery of cellulase in the digestive tract of snails, the microbial degradation of cellulose has attracted enough attention in the industry. Now the application of cellulase has been extended to various fields such as food fermentation, medicine, textile, daily chemical industry, papermaking, industrial washing, tobacco, oil extraction, wastewater treatment and feed, and its application prospect is very broad. [0003] With the increasingly prominent energy problems, countries gradually regard the degradation of cellulose as an important research topic. The enzymatic degradation of cellulose is an effective way to utilize cellulose. At present, the research on fungal cellulase has been very mature, and the activity of fungal cellulase is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/42C12R1/01
Inventor 朱红惠黎志坤羊宋贞冯广达王永红陈美标
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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