Application of antrodia camphorata cyclohexenone compound in preparing medicine for inhibiting growth of pancreatic cancer tumor cells
A tumor cell and compound technology, which is applied in the application field of Antrodia cinnamomea cyclohexenone compound in the preparation of a drug for inhibiting the growth of pancreatic cancer tumor cells, and can solve unpublished specific problems and the like
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Embodiment 1
[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separation
[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.
[0025] The Antrodia camphorata extract collected above was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 column (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) As mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is 10% % to 90%; 35...
Embodiment 2
[0028] Anti-pancreatic cancer tumor cell activity test in vitro
[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone Compounds were added to the culture medium of human pancreatic cancer tumor cells BxPC-3 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the conventional MTT assay method, and the pancreatic cancer tumor cell BxPC-3 is a human pancreatic adenocarcinoma cell line (ductal adenocarcinoma cell line).
[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide...
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