Application of antrodia camphorata cyclohexenone compound in preparing medicine for inhibiting growth of pancreatic cancer tumor cells

A tumor cell and compound technology, which is applied in the application field of Antrodia cinnamomea cyclohexenone compound in the preparation of a drug for inhibiting the growth of pancreatic cancer tumor cells, and can solve unpublished specific problems and the like

A tumor cell and compound technology, which is applied in the application field of Antrodia cinnamomea cyclohexenone compound in the preparation of a drug for inhibiting the growth of pancreatic cancer tumor cells, and can solve unpublished specific problems and the like

CN102000046BActive Publication Date: 2012-05-30GOLDEN BIOTECH
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  • Application of antrodia camphorata cyclohexenone compound in preparing medicine for inhibiting growth of pancreatic cancer tumor cells
  • Application of antrodia camphorata cyclohexenone compound in preparing medicine for inhibiting growth of pancreatic cancer tumor cells
  • Application of antrodia camphorata cyclohexenone compound in preparing medicine for inhibiting growth of pancreatic cancer tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separation

[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.

[0025] The Antrodia camphorata extract collected above was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 column (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) As mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is 10% % to 90%; 35...

Embodiment 2

[0028] Anti-pancreatic cancer tumor cell activity test in vitro

[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone Compounds were added to the culture medium of human pancreatic cancer tumor cells BxPC-3 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the conventional MTT assay method, and the pancreatic cancer tumor cell BxPC-3 is a human pancreatic adenocarcinoma cell line (ductal adenocarcinoma cell line).

[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide...

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Abstract

The invention relates to a new purpose of a compound. In the invention, antrodia camphorata extract is separated and purified to obtain 4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone. The cyclohexenone compound can be used for inhibiting growth of pancreatic cancer tumor cells and simultaneously can be used for pharmaceutical compositions for inhibiting growth of pancreatic cancer tumor cells.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from antrodia cinnamomea (Antrodia cinnamomea) extract to inhibit the growth of pancreatic cancer tumor cells. Background technique [0002] Pancreatic cancer is the fourth leading cause of cancer death in Western countries, and it is also the tenth among the top ten causes of cancer death in Taiwan. About 60% of pancreatic cancers occur in the head of the pancreas, and about 21% may invade the entire pancreas. Among exocrine pancreatic tumors, pancreatic ductal adenocarcinoma (pancreatic ductal adenocarcinoma) is The most common type, accounting for 85-90% of all pancreatic cancers. [0003] Pancreatic cancer is a malignant disease with strong aggressiveness and high fatality. The treatment of this kind of cancer mainly includes surgical treatment, chemotherapy and radiotherapy. % of patients have the opportunity to undergo su...

Claims

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Application Information

Patent Timeline
30 May 2012
Publication
CN102000046B
IPC
A61K31/122; A61P35/00
Inventors
刘胜勇; 温武哲