Klebsiella pneumoniae from mangrove forest and application thereof in producing 1,3-propylene glycol

A technology for Klebsiella and pneumonia, applied in the field of Klebsiella mangrove and its production 1, can solve the problems of fermentation and production, and achieve the effect of strong tolerance

Active Publication Date: 2012-07-25
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented bacteria called Klebsiela Pneumonias (KP) can produce high amounts of propane-1-one that are resistant against certain substances found naturally throughout ocean waters worldwide. These unique properties make them useful in various industries such as medicine or biotechnology researches where they could be used to study natural processes like nutrients transportation within sea water systems.

Problems solved by technology

This patents discusses different methods for making certain types of compounds called diols like 3-, 4'-dimmers of hexacycloheptane through metabolism chemistry processes involving various yeast generators. However, these techniques require expensive starting materials and complex operations. Therefore, they limit their use because of economic limitations associated with large amounts of waste generated during manufacturing. To address these issues, researchers developed genetically engineered crops containing genome sequences coding specific components instead of randomly distributed sources. These crop plants could potentially provide valuable biofuels and pharmaceuticals while reducing greenhouse gas emissions compared to fossil fuel oil. Additionally, the technical problem addressed in this patent is how efficiently convert crude hydroxyaccharides into industrially useful molecules via beta cycling reactions under controlled environments overcomes challenges related to conventional methods including laborious procedures and poor product quality caused by volatile impurities.

Method used

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  • Klebsiella pneumoniae from mangrove forest and application thereof in producing 1,3-propylene glycol
  • Klebsiella pneumoniae from mangrove forest and application thereof in producing 1,3-propylene glycol
  • Klebsiella pneumoniae from mangrove forest and application thereof in producing 1,3-propylene glycol

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Embodiment 1

[0020] 1. Isolation and identification of bacterial species

[0021] 1. Sampling: The samples were collected from the surface sediment of the mangrove forests in the mangrove park in Shenzhen City, Guangdong Province after the tide water receded. After the samples were collected, they were put into sterilized polyethylene tubes and brought back to the laboratory for immediate processing.

[0022] 2. Strain isolation and screening:

[0023] (1) Bacterial isolation: mix the sample with phosphate buffered saline (PBS) at a mass ratio of 1:50, let it stand for 30 minutes, discard the lower precipitate, collect the supernatant, centrifuge at 5000g for 10 minutes, take the precipitate, and rewet it with 1ml PBS. suspended sediment. Take 100 μl of the liquid and spread it on MIAC, a high-efficiency Klebsiella isolation medium, and incubate at 37°C for 18-24 hours. The preparation method of MIAC medium is as follows: peptone 20.0g, inositol 5.0g, adonol 5.0g, sodium chloride 5.0g, p...

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Abstract

The invention discloses a Klebsiella pneumoniae from mangrove forest and application of the Klebsiella pneumoniae in producing 1,3-propylene glycol. The Klebsiella pneumoniae HSL4 is reserved in the China Center for Type Culture Collection (CCTCC) on March 21, 2011 in Wuhan University in China and the preservation number is CCTCC NO: M 2011075. The Klebsiella pneumoniae HSL4 separated and screened from the ocean mangrove forest ecological system is a new strain of the Klebsiella pneumoniae and has relatively strong production capacity of 1,3-propylene glycol; and the strain is separated from the ocean mangrove forest ecological system and has relatively strong tolerance to the organic acid. Thus, the Klebsiella pneumoniae HSL4 has potential utilization value in the industry production.

Description

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Claims

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Application Information

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Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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