Acinetobacter baumannii enriched culture medium

An Acinetobacter baumannii, enrichment culture technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as long cycle, and achieve the effect of fast growth and fast cultivation.

Active Publication Date: 2014-03-26
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The key to the promotion and application of Acinetobacter baumannii is the need for a suitable culture medium. The current classic conventional medium for the enrichment of Acinetobacter baumannii is used. The average enrichment time is 35-45h, and the cycle is long.

Method used

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  • Acinetobacter baumannii enriched culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of medium enriched for Acinetobacter baumannii

[0019] Basal medium: 3.8g dipotassium hydrogen phosphate, 1g potassium dihydrogen phosphate, 1g sodium chloride, 0.1g ammonium chloride, 0.2g magnesium sulfate, 2g glucose, made into 1000ml with distilled water.

[0020] In the above basal medium, add 2.5g of yeast extract and 1.5g of hydroxysuccinic acid.

[0021] (2) Culture of Acinetobacter baumannii

[0022] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Acinetobacter baumannii was inoculated in the culture medium at an inoculation amount of 5%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0023] Enrichment culture results: After 28 hours of constant temperature culture, the OD of the bacterial solution 600 1.8, after 30 hours of constant temperature cultivation, the maximum growth amount was reached, and the OD of the bacterial solution 600 1.9, the maximum growt...

Embodiment 2

[0025] (1) Preparation of medium enriched for Acinetobacter baumannii

[0026] Basal medium: 3.8g dipotassium hydrogen phosphate, 1g potassium dihydrogen phosphate, 1g sodium chloride, 0.1g ammonium chloride, 0.2g magnesium sulfate, 2g glucose, made into 1000ml with distilled water.

[0027] In the above basal medium, add 0.5g yeast extract and 0.5g hydroxysuccinic acid.

[0028] (2) Culture of Acinetobacter baumannii

[0029] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Acinetobacter baumannii was inoculated in the culture medium at an inoculation amount of 5%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0030] Enrichment culture results: After 28 hours of light culture, the bacterial solution OD 600 1.75, after 31 hours of light culture, the maximum growth amount was reached, and the OD of the bacterial solution 600 1.9, the maximum growth of Acinetobacter baumannii was advanced by 5...

Embodiment 3

[0032] (1) Preparation of medium enriched for Acinetobacter baumannii

[0033] Basal medium: 3.8g dipotassium hydrogen phosphate, 1g potassium dihydrogen phosphate, 1g sodium chloride, 0.1g ammonium chloride, 0.2g magnesium sulfate, 2g glucose, made into 1000ml with distilled water.

[0034] In the above basal medium, add 4.0 g of yeast extract and 2.0 g of hydroxysuccinic acid.

[0035] (2) Culture of Acinetobacter baumannii

[0036] Adjust the pH of the above medium to 7, and sterilize at 121°C for 30 minutes. Acinetobacter baumannii was inoculated in the culture medium at an inoculation amount of 5%, oscillated and mixed, and cultured in a constant temperature incubator at a temperature of 30°C.

[0037] Enrichment culture results: After 28 hours of light culture, the maximum growth amount was reached, and the OD of the bacterial solution 600 1.9, the maximum growth of Acinetobacter baumannii was advanced by 5h to 7h.

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Abstract

The invention relates to an Acinetobacter baumannii enriched culture medium, comprising a basic medium, extract yeast and hydroxy succinic acid, wherein 0.5-4.0 g of extract yeast and 0.5-2.0 g of hydroxy succinic acid are added in every 1000 ml basic medium. The application of the culture medium in culturing Acinetobacter baumannii has quick culture speed and low cost. According to the invention, the shortages of slow growth of Acinetobacter baumannii and the like are made up for, the growth speed is raised, and the time of getting the maximum quantity is shortened by 5-7 h.

Description

technical field [0001] The invention relates to a culture medium for enriching bacteria, in particular to a culture medium for enriching Acinetobacter baumannii. Background technique [0002] Acinetobacter baumannii is a kind of non-fermentative conditional pathogenic bacteria widely distributed in the natural environment and hospitals, and exists in the skin, oral cavity, respiratory tract, gastrointestinal tract and urinary tract of the human body. Because the bacterium generally only causes illness in people with weakened immune function, it is often considered a non-pathogenic bacterium or an opportunistic pathogenic bacterium. In recent years, the number of infections caused by it has been increasing, and the drug resistance has become increasingly serious, which has attracted serious attention from clinical and microbiologists. [0003] Acinetobacter baumannii is a species belonging to the genus Acinetobacter, which is a non-saccharide-fermenting, oxidase-negative, no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 徐成斌马溪平李瑶瑶邵亮孟雪莲包坤牟安逸
Owner LIAONING UNIVERSITY
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