The invention discloses a transfer
plasmid for transforming
Acinetobacter baumannii, which comprises, in
clockwise order, a
replicon ori, an
ampicillin resistance
gene AmpR, a transposon sequence, a junction transfer
initiation site oriT, the transposon sequence contains a
base sequence capable of autonomously emitting
Acinetobacter baumannii and a resistance
gene, and both ends of the resistancegene are provided with DifR and DifL sequences; also disclosed is an autonomously illuminated
Acinetobacter baumannii without a resistance marker, the
gene of
Acinetobacter baumannii containing a genecapable of expressing autonomous light-emitting
protein, and no resistance screening gene is in the
genome of
Acinetobacter baumannii. In the present invention, the autonomously illuminated
Acinetobacter baumannii, which is constructed by the transfer
plasmid pUC18T-mini-Tn7T-
lux-Ab-dif-Apr and the auxiliary
plasmid pTNS3, can emit light without adding any substrate; and luminescent colonies canbe seen with the
naked eye in a dark environment.