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Alternaria alternata inoculating liquid and preparation method thereof

A technology of tobacco Alternaria inoculum and inoculum, applied in the field of tobacco Alternaria inoculum and its preparation, capable of solving the problems of low incidence of tobacco plants and the like

Inactive Publication Date: 2014-04-30
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a tobacco Alternaria inoculum and its preparation method, aiming to solve the problem of low incidence of tobacco plants caused by the existing field inoculum

Method used

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Examples

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Embodiment 1

[0010] A method for preparing an inoculum of Tobacco scab bacteria, comprising the following steps: after culturing the tobacco scab bacteria on a PDA plate at 28 DEG C for 7 days, using 0.5% (v / v) glucose solution, 1.5% (v / v) Tween- 20 into 0.5×10 6 The spore suspension of cfu / ml is the inoculum 1 of Tobacco scab.

Embodiment 2

[0012] A method for preparing an inoculum of Tobacco scab, comprising the following steps: after culturing the Tobacco scab on a PDA plate at 28 DEG C for 6 days, using 1.5% (v / v) glucose solution, 0.5% (v / v) Tween- 20 into 1.5×10 6 The spore suspension of cfu / ml is the inoculum 2 of Tobacco scab.

Embodiment 3

[0014] A method for preparing an inoculum of Tobacco scab, comprising the following steps: after culturing the Tobacco scab on a PDA plate at 28°C for 7 days, using 1% (v / v) glucose solution, 1% (v / v) Tween- 20 into 1×10 6 The spore suspension of cfu / ml is the inoculum 3 of Tobacco scab.

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PUM

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Abstract

The invention provides an alternaria alternata inoculating liquid and a preparation method thereof. The preparation method comprises the following steps: culturing the alternaria alternata on a PDA (Potato Dextrose Agar) panel for 6-7 days at 28 DEG C; preparing a 1*10<6>cfu / ml spore suspension liquid by a 0.5-1.5% (v / v) glucose liquid and 0.5-1.5% (v / v) tween-20 to obtain the alternaria alternata inoculating liquid. The alternaria alternata inoculating liquid provided by the invention is directly sprayed in the field to inoculate so as to effectively improve the morbidity of tobacco strains. In addition, the preparation method provided by the invention is simple and practical and easy to operate and popularize.

Description

technical field [0001] The invention belongs to the field of tobacco brown spot bacteria inoculation, in particular to a tobacco brown spot bacteria inoculation solution and a preparation method thereof. Background technique [0002] The field inoculation method of existing Tobacco scab pathogens is usually directly inoculated by spraying the spore suspension of Tobacco scab on tobacco strains. This inoculation method has low adhesion of spores on tobacco strains and is susceptible to weather restrictions. For example, in rainy days, the spores are easily washed away by the rain, resulting in a low incidence of tobacco plants. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to provide an inoculum of Tobacco scab and a preparation method thereof, aiming to solve the problem of the low incidence of tobacco strains caused by the inoculum in the field. [0004] The present invention is achieved in this way, a tobacco scab bacteria inoculation solutio...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 于海芹
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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