Alternaria alternata inoculation liquid and preparation method thereof

A technology of tobacco Alternaria inoculum and inoculum, which is applied in the field of tobacco Alternaria inoculum and its preparation, and can solve the problems of low incidence of tobacco plants and the like

Inactive Publication Date: 2014-04-16
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a tobacco Alternaria inoculum and its preparation method, aiming to solve the problem of low incidence of tobacco plants caused by the existing field inoculum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] A kind of preparation method of tobacco Alternaria inoculum, comprising the following steps: After culturing tobacco Alternaria on a PDA plate at 28°C for 7 days, use 0.5% (v / v) glucose solution, 1.5% (v / v) spit Wen-20 dubbed O.5×10 6 The spore suspension of cfu / ml is the inoculum of Alternaria Alternaria.

Embodiment 2

[0012] A preparation method for an inoculum of tobacco red spot bacteria, comprising the following steps: after culturing tobacco red spot bacteria on a PDA plate at 28°C for 6 days, using 1.5% (v / v) glucose solution, 0.5% (v / v) spit Wen-20 dubbed 1.5×10 6 The spore suspension in cfu / ml is the inoculum 2 of Alternaria tabacum.

Embodiment 3

[0014] A method for preparing an inoculum of Alternaria tabacum, comprising the following steps: after culturing Alternaria tabacum on a PDA plate at 28° C. for 7 days, use 1% (v / v) glucose solution, 1% (v / v) Tween- 20 dubbed into 1×10 6 The cfu / ml spore suspension is the tobacco Alternaria inoculum 3.

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Abstract

The invention provides alternaria alternata inoculation liquid and a preparation method thereof. The preparation method comprises the following steps: culturing alternaria alternata on a PDA (potato dextrose agar) plate at 28 DEG C for 6-7 hours, and preparing 1*10<6>cfu/ml of spore suspension liquid, namely the alternaria alternata inoculation liquid, from 0.5%-1.5% (v/v) of glucose liquid and 0.5%-1.5% (v/v) of tween-20. According to the alternaria alternata inoculation liquid, the incidence rate of tobacco plants can be effectively increased through direct field spraying inoculation; in addition, the preparation method is simple, practical, and easy to operate and popularize.

Description

technical field [0001] The invention belongs to the field of tobacco Alternaria inoculation, in particular to a tobacco Alternaria inoculum and a preparation method thereof. Background technique [0002] The field inoculation method of existing tobacco brown spot fungus usually all directly inoculates by spraying the spore suspension of tobacco brown spot fungus on the tobacco plants. This inoculation method has low adhesion of the spores on the tobacco plants and is easily restricted by the weather. For example, in rainy days, the spores are easily washed away by rainwater, resulting in a low incidence of tobacco plants. Contents of the invention [0003] The object of the present invention is to provide a tobacco Alternaria inoculum and a preparation method thereof, aiming at solving the problem of low disease incidence of tobacco plants caused by the existing field inoculum. [0004] The present invention is achieved in this way, a kind of tobacco Alternaria inoculum, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 于海芹
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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