Quick detection method and detection kit for hydrazoate in feed

A technology of azide and nitride, which is applied in the field of rapid detection of azide in feed and detection kits, can solve the problems of no detection method for azide in feed, and achieve the convenience of visual determination and on-site rapid determination, Brilliant results with high sensitivity

Inactive Publication Date: 2014-05-07
谱尼测试集团股份有限公司
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows quick and accurate testing of nitrogen compounds (NCS). It can be used with both fastest methods such as gas chromatography or liquid phase extraction but also faster methods like spectrophotometry that are more reliable than these techniques alone.

Problems solved by technology

The technical problem addressed by this patented research relates to improving animal husbandry and meat production without causing environmental concerns due to excessive use of pesticides like synthetic fertilizers. Existing monitoring systems require expensive equipment which may be difficult to install at remote locations where these substances could pose hazards during manufacturing processes. Therefore, an urgent need exists for fast and effective ways to identify any impurities added into feeds used for human consumption.

Method used

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  • Quick detection method and detection kit for hydrazoate in feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of standard color chart

[0045] Analysis steps: Weigh 1g of negative compound feed (accurate to 0.01g) into seven 100mL colorimetric tubes, then weigh sodium azide 0mg, 0.31mg, 1.55mg, 3.10mg, 7.74mg, 15.48mg, Add 30.95mg into a 100mL colorimetric tube, add 50mL of distilled water respectively, shake and stand still, take the supernatant and filter it through a 0.45μm water filter membrane, collect the filtrate; take 7-8mL of the filtrate into a 25mL colorimetric tube, add 5mol / L Adjust the pH value to 4 with hydrochloric acid; add 1mL of 1mol / L ferric chloride color reagent, dilute to 10mL with the filtrate, mix well, and develop color at room temperature for 10min, corresponding to 0.0g / kg azide root content in the feed , 0.2g / kg, 1.0g / kg, 2.0g / kg, 5.0g / kg, 10.0g / kg, 20.0g / kg. Take a picture of the color-developed solution, collect the color and make it into a standard color card, see figure 1 .

Embodiment 2

[0046] Embodiment 2: Azide Rapid Determination Kit

[0047] The kit includes: plastic sample spoons, 2 pieces, and the feed weight of one level spoon is about 1g. 3mL plastic dropper with graduation, 100 pcs; 25mL colorimetric tube, 30 pcs; 100mL colorimetric tube, 30 pcs; 50mL graduated cylinder, 1 pc; ; 0.45μm water filter membrane, 100 pieces; 5mL syringe, 100 pieces; precision pH test paper (0.5-5.0), 2 boxes; standard color card, 1 piece, a series of color cards with gradient changes from light yellow to blood red, A total of 7 items, the content of azide radicals corresponding to each item; reaction solution: 5mol / L hydrochloric acid solution (500mL, glass bottle), 1mol / L ferric chloride solution (500mL, glass bottle), 1000μg / mL nitrous acid Standard solution (20mL, plastic bottle); 500mL washing bottle, 1 piece.

Embodiment 3

[0049]Analysis steps: Take a spoonful of a certain brand of milk cow concentrate feed supplement in a 100mL volumetric flask, add 50mL of distilled water, shake and stand still, take the supernatant and filter it through a 0.45μm water filter membrane, and collect the filtrate; take 7-8mL of the filtrate to 25mL Color tube, adjust the pH value to 4 with 5mol / L hydrochloric acid solution, add 1mL of 1mol / L ferric chloride color reagent, dilute the filtrate to 10mL, mix well, develop color at room temperature for 10min, and appear light orange-red. Take another 7-8mL filtrate, add 1mL each of 5mol / L hydrochloric acid solution and 1000μg / mL nitrous acid standard reagent, if no color occurs, it means that there is no interference of thiocyanate in the sample, and the sample contains azide radical qualitatively. The color of the sample developed by ferric chloride is compared with the standard color card, which is close to the color card of 2.0g / kg. The azide root in the sample is e...

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Abstract

The invention discloses a quick detection method and detection kit for hydrazoate in a feed. The detection method comprises the following steps: adding standard hydrazoate into the blank substrate feed, adding distilled water, oscillating and performing still standing, and taking supernatant fluid to pass through a film; adding filtrate in a color comparison tube, adjusting pH value to be acidic, and adding a color developing reagent for color development; according to the obtained color developing photos, preparing a series of standard colorimetric cards with different hydrazoate concentrations. The detection kit comprises a plastic sample spoon, a plastic burette, a color comparison tube, a measuring tube, a test tube shelf, a 0.45-[mu]m water system filtration film, an injection syringe, pH test paper, standard colorimetric cards, a hydrochloric acid solution, an iron trichloride color developing reagent, a nitrous acid standard solution and a wash bottle. According to the invention, a test is carried out on a sample, the observed color is compared with the standard colorimetric cards for qualitative and semi-quantitative detection, if the color is straw yellow, no hydrazoate is doped in the sample; if the color is orange or sanguine, a verification test is required to be carried out; if the color remains the same, the hydrazoate is doped in the feed. The method and the kit are quick in color development reaction, not strict in measuring conditions, high in sensitivity and low in cost, and are particularly suitable for field test.

Description

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Claims

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Application Information

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Owner 谱尼测试集团股份有限公司
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