Marker for detecting pancreatic cancer

A pancreatic cancer and marker technology, applied in the field of pancreatic cancer detection, can solve the problems of sufficient sensitivity or specificity

Inactive Publication Date: 2015-03-04
NITTO BOSEIKI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these markers are not necessarily sufficient in terms of sensitivity or specificity, and furthermore, the development of new pancreatic cancer markers is required.

Method used

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  • Marker for detecting pancreatic cancer
  • Marker for detecting pancreatic cancer
  • Marker for detecting pancreatic cancer

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0031] Next, the present invention will be described in detail.

[0032] It is known from the present invention that for the serum test samples collected from healthy persons, chronic pancreatitis patients and pancreatic cancer patients (before and after surgery), the specificity in the serum of pancreatic cancer is carried out by using glycoprotein extraction and TMT reagent quantification. As a result of the identification of the sex glycoprotein, compared with the sera from healthy persons, patients with chronic pancreatitis, and pancreatic cancer patients (after surgery), there is a high level of C4BPA as a glycoprotein in the serum from pancreatic cancer patients (before surgery) or PIGR, thus finding that C4BPA and PIGR can be markers of pancreatic cancer.

[0033] The markers for detecting pancreatic cancer of the present invention include C4BPA or PIGR. C4BPA, the C4b-binding protein alpha chain, is a glycoprotein represented by the UniProt accession number P04003 (ht...

Embodiment 1

[0060] 【Identification of pancreatic cancer markers】

[0061] The serum test samples collected from 45 healthy persons, 25 patients with chronic pancreatitis, and 57 patients with pancreatic cancer were used to explore and identify pancreatic cancer markers.

[0062] 【A. Method】

[0063] [1. Subject's serum]

[0064] Serum test samples collected from 45 healthy subjects, 25 patients with chronic pancreatitis, and 57 patients with pancreatic cancer were used as serum for the exploration and identification of pancreatic cancer markers.

[0065] [2. Extraction of glycoproteins from each pooled serum]

[0066] The sera of 5 healthy persons, patients with chronic pancreatitis, and patients with pancreatic cancer (before operation-after operation) were randomly extracted and combined to obtain 3 types of combined sera.

[0067] Using a fucose-specific lectin, VECTREX AAL binding and elution kit, which is a retrieval kit for glycoproteins utilizing A. aurantia aurantia lectin (AAL...

Embodiment 3

[0089] [Usefulness of C4BPA and PIGR as pancreatic cancer markers]

[0090] The usefulness of C4BPA and PIGR as pancreatic cancer markers was verified by measuring C4BPA or PIGR in the test sample by ELISA using anti-C4BPA antibody or anti-PIGR antibody. In addition, the usefulness of C4BPA and PIGR as pancreatic cancer markers was also verified by evaluation using ROC curves, which are generally performed, for diagnostic tests or screening tests and comparisons with precision evaluation or conventional methods.

[0091] [1. Measurement of C4BPA or PIGR by ELISA]

[0092] 【(1) Method】

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Abstract

A marker for detecting pancreatic cancer, said marker comprising a glycoprotein C4BPA or PIGR, whereby a pancreatic cancer patient can be distinguished from a chronic pancreatitis patient or a normal subject and thus specifically diagnosed. Also, the marker is usable in monitoring the postoperative prognosis of a pancreatic cancer patient. When the aforesaid marker is used in combination with another pancreatic cancer marker such as CA19-9, furthermore, a pancreatic cancer patient can be distinguished from a chronic pancreatitis patient or a normal subject and diagnosed more specifically.

Description

【Technical field】 [0001] The present invention relates to markers for detecting pancreatic cancer. More specifically, the present invention relates to a marker for detecting pancreatic cancer for distinguishing it from chronic pancreatitis or a healthy person, a method for measuring a specific glycoprotein for detecting pancreatic cancer, a method for detecting pancreatic cancer, a specific Use of glycoprotein as a marker for detection of pancreatic cancer, a kit for detection of pancreatic cancer, etc. 【Background technique】 [0002] Malignant tumors in the hepatobiliary and pancreatic regions, especially pancreatic cancer, are known to have the poorest prognosis among digestive organ cancers. The main reason for this is that they are easy to infiltrate and metastasize to surrounding organs, so they are already diagnosed. Sexual carcinoma, so the resection rate is low, moreover, effective adjuvant therapy has not been established. Therefore, in order to increase the effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574C07K14/47
CPCC07K14/4748G01N33/57438G01N33/68
Inventor 野田健太三浦俊英野村文夫曾川一幸佐藤守吉富秀幸宫崎胜
Owner NITTO BOSEIKI CO LTD
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