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Application of melittin in preparation of drug used for inhibiting invasion metastasis of breast cancer cells

A breast cancer cell, melittin technology, applied in the field of bioengineering, can solve problems such as rarely achieving therapeutic effects

Inactive Publication Date: 2015-07-22
NORTHEAST NORMAL UNIVERSITY
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Problems solved by technology

Rarely achieves perfect treatment results

Method used

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  • Application of melittin in preparation of drug used for inhibiting invasion metastasis of breast cancer cells
  • Application of melittin in preparation of drug used for inhibiting invasion metastasis of breast cancer cells
  • Application of melittin in preparation of drug used for inhibiting invasion metastasis of breast cancer cells

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Embodiment Construction

[0018] 1. MCF-7 cell culture

[0019] Take out the frozen MCF-7 cells from the -80°C refrigerator, quickly transfer to a 37°C water bath to dissolve the cells, centrifuge at 800r for 5 minutes, and discard the supernatant. Suspend the cells in the frozen tube with 1ml of complete medium, centrifuge at 800r for 5 minutes, and discard the supernatant. Then suspend the cells with 1ml of complete medium, suck it into a 10 cm cell culture plate with a gun, add 7ml of complete medium, mix the cells, and place at 37°C, 5% CO 2 In a constant temperature incubator, culture until the cells cover the cell culture plate.

[0020] 2. Detection of CD147 protein by flow cytometry

[0021] Trypsinize the cells and add Staining medium to stop the digestion, centrifuge at 300g at 4°C for 5 minutes, label with IE-labeled anti-human CD147 antibody, and keep on ice for 30 minutes. Add 1ml of ice-cold Staining medium to rinse, centrifuge at 300g at 4°C for 5 minutes, repeat 3 times. Discard the...

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Abstract

The invention specifically relates to application of melittin in preparation of a drug used for inhibiting invasion metastasis of breast cancer cells, which belongs to the field of bioengineering technology. CD147 and MMP9 proteins exert significant effects on invasion and metastasis of breast cancer MCF-7 cells. The invention aims to find a CD147 expression inhibitor which is capable of hindering or reducing secretion of MMP9 and inhibiting invasion metastasis of cancer cells to cancerous peripheral tissue. According to the invention, MCF-7 cells are cultured at first, then different doses of melittin is used, and flow cytometry, ELISA and RT-PCR experimental results prove that expression of CD147 and MMP9 is suppressed in the MCF-7 cells treated by melittin; and then Transwell invasion chamber experiments are conducted to detect the migration rate of the MCF-7 cells treated by melittin. Melittin can inhibit invasion metastasis of the MCF-7 cells. Thus, a novel anti-breast cancer drug can be developed from melittin.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to the application of melittin in the preparation of drugs for inhibiting invasion and metastasis of breast cancer cells. Background technique [0002] Breast cancer is the most common female fatal tumor disease worldwide. In 2012, there were about 227,000 new cases of breast cancer in the United States, and 39,500 deaths, accounting for 17.4% of female cancer deaths. In recent years, the growth rate of the incidence of breast cancer in my country is higher than that of other countries. According to the "China Breast Disease Survey Report" issued by the China Population Association, the incidence of breast cancer ranks first among female tumors in large cities, accounting for 5.387 per 10,000 people. . It has become a major disease that threatens the health of women in our country. [0003] Cancer cell metastasis is the main cause of death in breast cancer patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P35/04
Inventor 霍洪亮王建军彭雪微李凤玉
Owner NORTHEAST NORMAL UNIVERSITY
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