Ovarian cancer multi-drug resistant cell line induced by triptolide and its application

A technology of triptolide and multidrug resistance, which is applied in the field of tumor biology and can solve problems affecting the degree of drug resistance of tumor cells
CN104805057BActive Publication Date: 2018-03-09HARBIN MEDICAL UNIVERSITY
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
HARBIN MEDICAL UNIVERSITY
Publication Date
2018-03-09

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a multidrug-resistant ovarian cancer cell line induced and established by triptolide and use thereof. The cell line is named SKOV3 / TPL, and the microorganism preservation number is CGMCC No. 10306. In the parental cells, the concentration of Triptolide (TPL), a monomeric extract of the traditional Chinese medicine Triptolide, was gradually increased from 10nM to 400nM, and the multidrug-resistant ovarian cancer cell line SKOV3 / TPL was established. The multidrug-resistant ovarian cancer cell line SKOV3 / TPL can stably grow, passage and recover in 400nM TPL, and its resistance index (RI) to TPL is 50.95. ‑FU, Ara‑C, GEM, VP‑16, MMC, ADM, MIT, TAX, VCR have cross-resistance. The establishment of the multidrug-resistant ovarian cancer cell line of the present invention provides a drug-resistant tumor cell model for tumor-related research, and the action mechanism of the drug can be studied by comparing its genetic difference with the parental cell.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a drug-resistant human ovarian cancer cell line and use thereof, in particular to the establishment of multi-drug resistance of ovarian cancer by gradually increasing the action concentration of a chemotherapeutic drug triptolide (TPL) by using human ovarian cancer SKOV3 as a parental cell. The cell line SKOV3 / TPL belongs to the field of tumor biology. Background technique

[0002] In the latest worldwide statistics on the incidence of tumors, the incidence of ovarian cancer ranks third among the malignant tumors of female reproductive organs, and the mortality rate ranks first, which seriously endangers women's health and life. Chemotherapy is one of the important means of ovarian cancer treatment and plays an important role in the comprehensive treatment of ovarian cancer, but clinical chemotherapy resistance is the main reason for the failure of ovarian cancer chemotherapy. The development of drug resistance is multifactori...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More