A kind of preparation method and application of rice bran polysaccharide with improved antioxidant activity
A rice bran polysaccharide and anti-oxidation technology, which is applied in the field of preparing fermented beverages, can solve the problems of interfering with the separation and purification of rice bran polysaccharides, long growth cycle, etc., and achieve the goal of improving the utilization rate of rice bran, increasing the antioxidant activity, and improving the properties of polysaccharides Effect
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Embodiment 1
[0022] Example 1 Preparation of rice bran polysaccharide with improved antioxidant activity
[0023] 1. The preparation of rice bran meal aqueous extract, concrete steps are as follows:
[0024] Take 20g of rice bran meal, add 200mL of distilled water and mix well, irradiate at a microwave frequency of 400W for 2 minutes, then boil and extract at 100°C for 20 minutes, and cool to 60°C. Add 0.1g amylase (3000U / mg) and 0.04mL glucoamylase (100000U / mL) to treat for 4 hours, boil at 100°C for 10 minutes to inactivate, centrifuge at 4000 rpm for 20 minutes, take the supernatant, and sterilize at 115°C for 30 minutes spare.
[0025] 2. Lactobacillus plantarum and Lactobacillus casei respectively ferment and treat the water extract of rice bran meal, the steps are as follows:
[0026] The Lactobacillus plantarum and Lactobacillus casei used were derived from purchased commercial strains, the number of the Lactobacillus plantarum strain was LP-Z01, and the number of the Lactobacillu...
Embodiment 2
[0036] Example 2 Fermentation of Lactobacillus plantarum LP-Z01 and Lactobacillus casei LC-G01 in MRS medium and analysis of antioxidant activity of polysaccharides before and after fermentation
[0037] 1. Inoculate Lactobacillus plantarum LP-Z01 and Lactobacillus casei LC-G01 into fresh solid MRS medium for activation for 12 hours, the activation temperature is 37°C, and the shaker speed is 200 rpm. The cultivated seed culture liquid was inoculated into 120 mL of MRS liquid medium according to the inoculation amount of 2% by volume, and cultivated for 72 hours at a culture temperature of 37° C. and a shaker speed of 120 rpm.
[0038] 2. Take 100mL of MRS medium treated with the above two kinds of Lactobacillus, add 400mL of absolute ethanol, precipitate at 4°C for 24 hours, centrifuge at 4000 rpm for 20 minutes to collect the precipitate, freeze-dry, and the obtained solid is polysaccharide.
[0039] 3. Take the polysaccharide prepared in step 2, and prepare the solution to ...
Embodiment 3
[0044] Example 3 Preparation of Fermented Beverage from Lactobacillus plantarum LP-Z01 Rice Bran Dregs Fermentation Liquid
[0045] 1. The preparation of rice bran meal aqueous extract, concrete steps are as follows:
[0046] Take 15g of rice bran meal, add 200mL of distilled water and mix well, irradiate at a microwave frequency of 300W for 6 minutes, then boil and extract at 100°C for 40 minutes, and cool to 60°C. Add 0.1g amylase (3000U / mg) and 0.04mL glucoamylase (100000U / mL) to treat for 6 hours, boil at 100°C for 10 minutes to inactivate, centrifuge at 4000 rpm for 20 minutes, take the supernatant, and sterilize at 115°C for 30 minutes spare.
[0047] 2. Inoculate the activated Lactobacillus plantarum LP-Z01 seed culture solution into 100mL of the above-mentioned rice bran meal water extract at 37°C for 72 hours at an inoculum volume of 3% by volume, then take it out and cool it, and post-ripen it at 4°C for 48 hours , and then centrifuge at 3000 rpm for 15 minutes to ...
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