A kind of human fructose diphosphate aldolase b gene detection method and kit

A diphosphate aldolase and gene detection technology, which is applied in the field of genetic engineering, can solve the problems of radiographic imaging, low resolution, and high technical requirements, and achieve the effects of reducing false positives, simplifying the operation process, and reducing operation steps

Active Publication Date: 2019-07-23
广东安科华南生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the operation is complicated, the operator has high technical requirements, radiography is required, the resolution is not high, and it takes a long time

Method used

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  • A kind of human fructose diphosphate aldolase b gene detection method and kit
  • A kind of human fructose diphosphate aldolase b gene detection method and kit
  • A kind of human fructose diphosphate aldolase b gene detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] ①Establishment of standard atlas

[0057] According to the above-mentioned use method, the control gene sequence is detected, and the following results are obtained: figure 1 and 2 The three-site detection map of the normal sequence compared with the wild-type specific primer and the three-site detection map of the mutant sequence with the mutant-type specific primer are shown;

[0058] ②Sample testing and comparison

[0059] Test No. 1 sample according to the above-mentioned method, and obtain the following results: image 3 As shown in the spectrum, it can be seen that sample No. 1 is a single point mutation at the A150P site.

Embodiment 2

[0061] Its overall flow process is identical with embodiment 1, and the testing result of No. 2 sample is as follows Figure 4 As shown, it can be seen that sample No. 2 has a single point mutation at the A175D site.

Embodiment 3

[0063] Its overall flow process is identical with embodiment 1, and the detection result of No. 3 sample is as follows Figure 5 As shown, it can be seen that sample No. 3 has a single point mutation at the N335K site.

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Abstract

Disclosed are a detection method and kit for the human fructose bisphosphate aldolase B gene. The detection kit comprises a primer pair for the specific amplification of three polymorphic sites on the human fructose bisphosphate aldolase B gene. The three polymorphic sites are respectively A150P, A175D and N335K. The primer pair comprises a consensus upstream primer and a typing downstream primer, and the typing downstream primer comprises a wild-type specific primer and a mutant specific primer. The kit can rapidly and efficiently detect genotypes of the three polymorphic sites of the human fructose bisphosphate aldolase B gene, and is a rapid, simple and convenient, economical, and efficient kit for screening the human fructose bisphosphate aldolase B gene.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a human fructose diphosphate aldolase B gene detection kit and a detection method thereof. Background technique [0002] Hereditary Fructose Intolerance (HFI) is a kind of fructose metabolism disease. It is an autosomal recessive genetic disease. Deficiency or reduced activity, resulting in abnormal fructose metabolism in patients. The incidence rate in Europe is about 1:26100, while the incidence rate in my country is still unclear. With the improvement of people's living standards, the consumption of sugar is increasing, and the incidence of HFI will gradually increase. [0003] Clinically, children with hereditary fructose intolerance are rare. Since most formulas contain sucrose, newborns who are fed artificially after birth can experience vomiting, diarrhea, dehydration, and shock within 2-3 days and bleeding tendency and other symptoms of acute liver failure....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/68
Inventor 杜蔚安黄源坚郑文彦高静王邦超彭百华周玉吴智锋
Owner 广东安科华南生物科技有限公司
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