Method for preparing cinnamomum cassia presl crude polysaccharides

A technology for crude polysaccharide and gold is applied in the field of preparation of crude polysaccharide from golden pear residue, which can solve the problems of unsatisfactory extraction rate, influence on product yield, low economic benefit, etc. Effect

Inactive Publication Date: 2017-06-27
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing polysaccharide preparation methods include water extraction, alkali extraction and enzymatic hydrolysis, etc., each has its advantages and disadvantages. Although the process conditio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] ⑴Preparation of raw materials: After extracting the golden pear flavor, the clean golden pear dregs are dried at low temperature, crushed and passed through a 50-mesh sieve, and used as raw materials;

[0022] ⑵Hot water treatment: Put the raw materials directly into the multi-functional extractor, add 15 times the amount of distilled water, stir evenly, extract at 85°C for 2.0 hours, filter and separate to obtain the first extraction filtrate, and add the residue 10 times the volume of distilled water, stirred

[0023] Evenly, extract at 95°C for 1.5h, filter and separate to obtain the second extraction filtrate; combine the two extraction filtrates;

[0024] (3) Enzyme hydrolysis treatment: Add 15 times the amount of residue to distilled water, stir evenly, add compound enzyme with 3% amount of golden pear residue (complex enzyme includes 1.5% serrapeptase and 1.5% papain), at a temperature of 55 ℃, pH 7, enzymatic hydrolysis for 1.5h, then rapidly heated to 98°C to ...

Embodiment 2

[0030] ⑴Prepare raw materials: after extracting golden pear flavor, clean golden pear dregs, dry at low temperature, crush and pass through a 60-mesh sieve, and use it as raw material;

[0031] (2) Hot water treatment: Put the raw materials directly into the multifunctional extractor, add 15 times the amount of distilled water, stir evenly, extract at 85°C for 1.5 hours, filter and separate to obtain the first extraction filtrate, and add the residue 10 times the amount of distilled water, stir evenly, extract at 95°C for 1 hour, filter and separate to obtain the second extraction filtrate; combine the two extraction filtrates;

[0032] (3) Enzymolysis treatment: add the residue to 15 times the amount of distilled water, stir evenly, add 4% compound enzyme of golden pear residue (complex enzyme includes 2% serrapeptase and 2% papain), at 50 ℃, pH 6.8, enzymatic hydrolysis for 1.5h, then rapidly heated to 98°C to inactivate the enzyme, and kept for leaching for 0.5h, centrifuge...

Embodiment 3

[0039] A kind of preparation method of golden pear dregs crude polysaccharide, its specific steps are:

[0040] ⑴Preparation of raw materials: After extracting the golden pear flavor, the clean golden pear dregs are dried at low temperature, crushed and passed through an 80-mesh sieve, and used as raw materials;

[0041] (2) Hot water treatment: Put the raw materials directly into the multifunctional extractor, add 15 times the amount of distilled water, stir evenly, extract at 85°C for 1.5 hours, filter and separate to obtain the first extraction filtrate, and add the residue 10 times the volume of distilled water, stirred

[0042] Evenly, extract at 95°C for 1 hour, filter and separate to obtain the second extraction filtrate; combine the two extraction filtrates;

[0043] (3) Enzyme hydrolysis treatment: add the residue to 15 times the amount of distilled water, stir evenly, add 5% compound enzyme of golden pear dregs (complex enzyme includes 2.5% serrapeptase and 2.5% pap...

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Abstract

The invention discloses a preparation method of golden pear dregs crude polysaccharide. The preparation method of golden pear dregs crude polysaccharide includes the following steps: preparing raw materials, hot water treatment twice, compound enzymatic hydrolysis treatment, concentration treatment, decolorization treatment, alcohol analysis Handling and freeze-drying. The present invention properly combines the traditional water extraction method and the new technology compound enzymatic hydrolysis method, and makes reasonable improvements, which not only reduces the loss of polysaccharides, correspondingly improves the extraction rate, but also improves the purity of polysaccharides, facilitates the subsequent polysaccharide purification process, and has mild process conditions, Features of low energy consumption and high efficiency.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a preparation method of golden pear dregs crude polysaccharide. Background technique [0002] Golden pear (Cinnamomum cassia Presl), also known as cinnamomum cassia, is a natural Chinese medicinal material. It has been used in traditional Chinese medicine and spices since ancient times. Golden pear skin and leaves can be used to extract golden pear oil. Golden pear oil has a unique aroma and smell, and can sterilize and deodorize. It has been widely used in daily chemicals, medicine, spices, food, pesticide chemicals and other fields; it is used as a condiment and food additive, and has the functions of strengthening the spleen and stomach and promoting appetite. [0003] my country's golden pear resources are very rich, with an annual output of more than 2,000 tons of osmanthus oil, and about 200,000 tons of golden pear residues (golden pear dregs)...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/00
Inventor宋容
Owner宋容