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Reagent for detecting tyrosine phenolic metabolites in human urine and preparation method thereof

A technology of tyrosine phenol and detection reagent, which is applied in the field of detection reagent and preparation of tyrosine phenolic metabolites in human urine, and can solve the problems of interfering with the determination of tyrosine and its metabolites and affecting the accuracy

Active Publication Date: 2020-06-16
湖南阿能医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The presence of proteins in biological samples can interfere with the determination of tyrosine and its metabolites, thereby affecting the accuracy of the detection

Method used

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  • Reagent for detecting tyrosine phenolic metabolites in human urine and preparation method thereof
  • Reagent for detecting tyrosine phenolic metabolites in human urine and preparation method thereof
  • Reagent for detecting tyrosine phenolic metabolites in human urine and preparation method thereof

Examples

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Effect test

preparation example Construction

[0043] In a preferred embodiment, the preparation method of the phosphomolybdotungstic acid-chitosan-nano silicon dioxide composite at least comprises the following steps:

[0044](1) Dissolve nano-silica in ethanol solution, ultrasonically disperse at room temperature for 60 minutes, add silane coupling agent K560, and stir at 45°C for 3 hours to obtain nano-silica solution; then dissolve chitosan in acetic acid solution , stirred at room temperature for 3 hours to obtain a chitosan solution; added the chitosan solution to the nano-silica solution, stirred and reacted for 8 hours, centrifuged and washed 3 times with water, washed 2 times with ethanol, and dried in vacuum at 60°C to obtain chitosan Sugar-nano silica complex.

[0045] (2) Dissolve phosphomolybdenum tungstic acid in deionized water first, prepare a solution with a mass fraction of 10% phosphomolybdenum tungstic acid, then add the chitosan-nanometer silicon dioxide complex obtained in step (1), and reflux at a co...

Embodiment 1

[0056] Embodiment 1 provides a detection reagent for tyrosine phenolic metabolites in human urine, including:

[0057]

[0058] The preparation method of described phosphomolybdotungstic acid-chitosan-nano-silicon dioxide composite comprises the following steps:

[0059] (1) Dissolve nano-silica in ethanol solution, ultrasonically disperse at room temperature for 60 minutes, add silane coupling agent K560, and stir at 45°C for 3 hours to obtain nano-silica solution; then dissolve chitosan in acetic acid solution , stirred at room temperature for 3 hours to obtain a chitosan solution; added the chitosan solution to the nano-silica solution, stirred and reacted for 8 hours, centrifuged and washed 3 times with water, washed 2 times with ethanol, and dried in vacuum at 60°C to obtain chitosan Sugar-nano silica complex.

[0060] (2) Dissolve phosphomolybdenum tungstic acid in deionized water first, prepare a solution with a mass fraction of 10% phosphomolybdenum tungstic acid, ...

Embodiment 2

[0068] Embodiment 2 provides a detection reagent for tyrosine phenolic metabolites in human urine, including:

[0069]

[0070] The preparation method of described phosphomolybdotungstic acid-chitosan-nano-silicon dioxide composite comprises the following steps:

[0071] (1) Dissolve nano-silica in ethanol solution, ultrasonically disperse at room temperature for 60 minutes, add silane coupling agent K560, and stir at 45°C for 3 hours to obtain nano-silica solution; then dissolve chitosan in acetic acid solution , stirred at room temperature for 3 hours to obtain a chitosan solution; added the chitosan solution to the nano-silica solution, stirred and reacted for 8 hours, centrifuged and washed 3 times with water, washed 2 times with ethanol, and dried in vacuum at 60°C to obtain chitosan Sugar-nano silica complex.

[0072] (2) Dissolve phosphomolybdenum tungstic acid in deionized water first, prepare a solution with a mass fraction of 10% phosphomolybdenum tungstic acid, ...

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to a detection reagent for phenolic metabolites of tyrosine in human urine and a preparation method of the detection reagent. The detection reagent for the phenolic metabolites of the tyrosine in the human urine at least comprises 1-20 mol / L of mercurous nitrate, 1-5 mol / L of mercury sulfate, 0.1-2 mol / L of nickel nitrate, 3-20 mol / L of sulfuric acid and 0.01-1 g / L of a phosphomolybdium tungstic acid-chitosan-nanometer silicon dioxide composition.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a detection reagent for tyrosine phenolic metabolites in human urine and a preparation method thereof. Background technique [0002] In recent years, the incidence of tumor diseases around the world has been on the rise. According to the statistics of cancer worldwide by the American Cancer Society, from 2008 to 2030, the number of new cancers worldwide will increase from 12.7 million per year Increased to 21.4 million, the number of cancer deaths will rise from 7.6 million to 13.2 million. At the same time, my country's statistics in recent years show that it is estimated that in 2020, the number of new cancer cases in China will reach 3.49 million, and the number of deaths due to malignant tumors will reach 2.63 million, an increase of 60% compared with 2002["8]. It can be seen that No matter in developed countries or developing countries, malignant tumors (cancers) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 朱建华
Owner 湖南阿能医疗器械有限公司
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