A kind of antigen detection material and its preparation method and application

Abstract

The invention provides a preparation method of an antigen detection material. The preparation method comprises the following steps: firstly, preparing a micron porous silicon substrate by using an electrochemical etching method, then performing surface modification on the porous silicon substrate, so that a composite single-layer film of two silanes containing hydrophobic long carbon and a reactive end group respectively is assembled on the porous silicon substrate, and finally grafting a specific antibody to the surface of the modified porous silicon substrate to generate a composite monomolecular layer, so that the surface becomes a superhydrophobic biological functional surface combined with the specific antibody. The superhydrophobic biological functional porous silicon material prepared by the preparation method provided by the invention can be used for detecting antigenic diseases, the principle is that the surface of the material containing the antibody can react with liquid containing the antigen to generate an adhesion force, and whether a to-be-tested sample contains a specific disease antigen is judged by detecting the presence and size of the adhesion force of the liquid drop of the to-be-tested sample. The detection material involved in the invention is simple in preparation process, convenient in detection process and high in actual application value.

Description

technical field

[0001] The invention relates to a method for modifying the surface of a porous silicon substrate, specifically, modifying the surface of the porous silicon substrate to make the surface a superhydrophobic biological functional surface bound with specific disease antibodies, utilizing its superhydrophobicity and Biological function The material can be used to quickly detect whether a specific disease antigen is contained in a test sample. Background technique

[0002] Silicon materials are widely used in biosensors, biochips, etc., and can be used as biosensors. In terms of antigen or antibody detection, a common method is to graft antigens / antibodies on the surface of porous silicon substrates. When detecting When the antibody / antigen in the sample binds to it, it will change the refractive index of the porous silicon film, thereby generating a response signal to sense the antibody. Detection methods based on porous silicon include: detection of reflection s...

Examples

Embodiment 1

[0029] A porous silicon-based material capable of detecting HIV-1 antigen in a blood sample is prepared and used to detect the blood sample to be tested.

[0030] A. Preparation of porous silicon-based materials that can detect hepatitis B surface antigen in blood samples:

[0031] (1) Preparation of microporous silicon substrate by electrochemical etching method:

[0032] Clean the silicon wafers: ultrasonically clean the single-sided polished silicon wafers with acetone and deionized water for 3 minutes, then dry them in the air; then put them into a mixed solution of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1 until No air bubbles are generated on the surface of the silicon wafer, the silicon wafer is taken out, rinsed with deionized water, soaked in 5% hydrofluoric acid aqueous solution for 1 min, then rinsed with deionized water, and blown dry with nitrogen.

[0033] Preparation of porous silicon by electrochemical etching method: use etha...

Embodiment 2

[0043] A porous silicon-based material capable of detecting hepatitis B surface antigen in blood samples is prepared and used to detect artificially prepared buffer solution samples containing hepatitis B surface antigen.

[0044] A. Preparation of porous silicon-based materials that can detect hepatitis B surface antigen in blood samples:

[0045] (1) Preparation of microporous silicon substrate by electrochemical etching method:

[0046] Clean the silicon wafers: ultrasonically clean the single-sided polished silicon wafers with acetone and deionized water for 3 minutes, then dry them in the air; then put them into a mixed solution of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1 until No air bubbles are generated on the surface of the silicon wafer, the silicon wafer is taken out, rinsed with deionized water, soaked in 5% hydrofluoric acid aqueous solution for 1 min, then rinsed with deionized water, and blown dry with nitrogen.

[0047] Prepa...

technical field

[0001] The invention relates to a method for modifying the surface of a porous silicon substrate, specifically, modifying the surface of the porous silicon substrate to make the surface a superhydrophobic biological functional surface bound with specific disease antibodies, utilizing its superhydrophobicity and Biological function The material can be used to quickly detect whether a specific disease antigen is contained in a test sample. Background technique

[0002] Silicon materials are widely used in biosensors, biochips, etc., and can be used as biosensors. In terms of antigen or antibody detection, a common method is to graft antigens / antibodies on the surface of porous silicon substrates. When detecting When the antibody / antigen in the sample binds to it, it will change the refractive index of the porous silicon film, thereby generating a response signal to sense the antibody. Detection methods based on porous silicon include: detection of reflection s...

Examples

Embodiment 1

[0029] A porous silicon-based material capable of detecting HIV-1 antigen in a blood sample is prepared and used to detect the blood sample to be tested.

[0030] A. Preparation of porous silicon-based materials that can detect hepatitis B surface antigen in blood samples:

[0031] (1) Preparation of microporous silicon substrate by electrochemical etching method:

[0032] Clean the silicon wafers: ultrasonically clean the single-sided polished silicon wafers with acetone and deionized water for 3 minutes, then dry them in the air; then put them into a mixed solution of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1 until No air bubbles are generated on the surface of the silicon wafer, the silicon wafer is taken out, rinsed with deionized water, soaked in 5% hydrofluoric acid aqueous solution for 1 min, then rinsed with deionized water, and blown dry with nitrogen.

[0033] Preparation of porous silicon by electrochemical etching method: use etha...

Embodiment 2

[0043] A porous silicon-based material capable of detecting hepatitis B surface antigen in blood samples is prepared and used to detect artificially prepared buffer solution samples containing hepatitis B surface antigen.

[0044] A. Preparation of porous silicon-based materials that can detect hepatitis B surface antigen in blood samples:

[0045] (1) Preparation of microporous silicon substrate by electrochemical etching method:

[0046] Clean the silicon wafers: ultrasonically clean the single-sided polished silicon wafers with acetone and deionized water for 3 minutes, then dry them in the air; then put them into a mixed solution of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1 until No air bubbles are generated on the surface of the silicon wafer, the silicon wafer is taken out, rinsed with deionized water, soaked in 5% hydrofluoric acid aqueous solution for 1 min, then rinsed with deionized water, and blown dry with nitrogen.

[0047] Prepa...