Selection method of brassica napus with glyphosate resistance and high linolenic acid content

Abstract

The invention discloses a selection method of brassica napus with glyphosate resistance and high linolenic acid content. The selection method of the brassica napus with the glyphosate resistance and the high linolenic acid content comprises the following steps: hybridizing brassica napus with glyphosate resistance with brassica napus with high linolenic acid content, and performing double screening for the glyphosate resistance and the high linolenic acid content on a large number of derived individual plants of progenies, namely selecting glyphosate-resistant strains with high linolenic acidcontent; and then, allowing several generations of selection so as to make related genotypes homozygous, so that the glyphosate resistance is consistent and stable among individual plants of the samefamily and the linolenic acid contents are stably higher than 15%, thereby obtaining the brassica napus with the glyphosate resistance and the high linolenic acid content. The brassica napus with thehigh linolenic acid content is adaptive to chemical weeding in virtue of the glyphosate resistance, so that lightened and simplified cultivation requirements can be adapted with cultivation benefits increased; and thus, immeasurable market application prospects are broadened.

Description

technical field [0001] The invention belongs to the field of cultivating new varieties of crops, in particular to a method for breeding high linolenic acid Brassica napus with resistance to the herbicide glyphosate. Background technique [0002] Human needs for a better life are increasingly demanding edible oils rich in omega-3 fatty acids. α-linolenic acid (symbol C18:3, English abbreviation LNA or ALA) is a representative plant source ω-3 fatty acid. Animals (such as deep-sea fish and shrimp) and humans can synthesize two other omega-3 fatty acids, EPA and DHA, when they ingest α-linolenic acid. EPA and DHA are respectively known as blood vessel scavenger and brain gold, and have very important health care functions for maintaining human health. α-linolenic acid is an essential fatty acid that cannot be synthesized by the human body but must be obtained from food. It is an indispensable component in the process of human metabolism and plays an important role in the grow...

AI Technical Summary

Problems solved by technology

The mEPSPS gene can be correctly expressed in each transgenic plant, but ther