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Peptides and nanoparticles for intracellular delivery of mRNA

A nanoparticle, cell-based technology applied in the field of peptide-containing complexes/nanoparticles

Pending Publication Date: 2020-08-07
AADIGEN LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their delivery properties can be challenging to engineer and improve

Method used

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  • Peptides and nanoparticles for intracellular delivery of mRNA
  • Peptides and nanoparticles for intracellular delivery of mRNA
  • Peptides and nanoparticles for intracellular delivery of mRNA

Examples

Experimental program
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Effect test

Embodiment approach 1

[0678] Embodiment 1. An mRNA delivery complex for intracellular delivery of mRNA comprising a cell penetrating peptide (CPP) and mRNA, wherein the cell penetrating peptide is selected from the group consisting of VEPEP-3 peptide, VEPEP-6 peptide, VEPEP-9 peptide and ADGN-100 peptide.

Embodiment approach 2

[0679] Embodiment 2. An mRNA delivery complex for intracellular delivery of mRNA comprising a cell penetrating peptide (CPP) and mRNA prepared by a method comprising: a) combining a first solution comprising mRNA with a solution comprising CPP The second solution is mixed to form a third solution, wherein the third solution comprises or is adjusted to comprise i) about 0-5% sucrose, ii) about 0-5% glucose, iii) about 0-50% DMEM, iv) about 0-80 mM NaCl, or v) about 0-20% PBS; and b) incubating the third solution to allow formation of the mRNA delivery complex.

Embodiment approach 3

[0680] Embodiment 3. The mRNA delivery complex of embodiment 2, wherein the first solution comprises mRNA in sterile water and / or wherein the second solution comprises CPP in sterile water.

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Abstract

The present invention pertains to peptide-containing complexes / nanoparticles that are useful for delivering into a cell one or more mRNA (such as therapeutic mRNA, e.g., mRNA encoding a tumor suppressor protein).

Description

[0001] related application [0002] This application claims the benefit of priority of French Application No. 1759645, filed October 16, 2017, and French Application No. 1853370, filed April 17, 2018, all of which are hereby incorporated by reference in their entirety for all purposes. [0003] Submit sequence listing on ASCII text file [0004] The following submissions on ASCII text files are incorporated herein by reference in their entirety: Computer Readable Form of the Sequence Listing (CRF) (File Name: 737372001042SEQLIST.txt, Date of Record: October 15, 2018, Size : 37KB). technical field [0005] The present invention relates to peptide-containing complexes / nanoparticles useful for delivering mRNA into cells. Background technique [0006] The disclosures of all publications, patents, patent applications, and published patent applications cited herein are hereby incorporated by reference in their entirety. [0007] In order for exogenous mRNA or RNAi to be therape...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/66C07K7/02A61K48/00C12N15/87C07K7/08
CPCC07K7/08C07K7/02C07K1/02C07K19/00C12N15/87A61K38/10A61K48/005A61K2121/00A61P35/00C07K14/003A61K47/64A61K48/0008A61K48/0033A61K9/51A61K38/465C07K14/00C12N15/1135C12Y301/03067A61K47/549A61K38/00
Inventor N·德赛G·迪维塔
Owner AADIGEN LLC
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