A method for constructing an animal model of kras mutation-associated malignant transformation of oral mucosa

Abstract

The invention relates to the field of life medicine, in particular to a method for constructing an animal model of oral mucosal malignant transformation related to Kras mutation. The method for constructing the mouse oral mucosal malignant transformation animal model of the present invention is as follows: using inducible Kras genetically engineered mice combined with 4-nitroquinoline 1-oxide (4NQO) chemical induction to construct, and taking the target that can form cancer as Under the premise, the modeling time should be shortened as much as possible. The traditional 4NQO chemically induced oral squamous cell carcinoma animal model has the disadvantage of taking a long time (≥20 weeks). The present invention uses Kras mutation combined with 4NQO to successfully induce oral squamous cell carcinoma, and it only takes 12 weeks from induction to cancer formation, which saves 2 months for the whole experiment compared with traditional animal models.

Description

technical field [0001] The invention relates to the field of life medicine, in particular to a method for constructing a Kras mutation-related oral mucosal malignant transformation animal model. Background technique [0002] About 30% of human tumors have reported Ras gene mutations, and in human pancreatic cancer, colon cancer, lung cancer, gallbladder cancer and liver cancer and other malignant tumors, the Ras gene mutation is as high as 30% to 90%. After the Ras gene mutation, the Ras protein is continuously activated, which leads to the continuous proliferation of cells and the occurrence of tumors. The most characteristic proteins of the Ras family mainly include three kinds of H-Ras, K-Ras and N-Ras. Most of the mutations in this family occur in the K-Ras gene. The common mutation sites are codon 12 and 13 of exon 2 of K-Ras gene, and codon 61 of exon 3, including G12D mutation. Currently, Kras G12D There are not many reports on the role of oral squamous cell carcin...

AI Technical Summary

Problems solved by technology

Although this method can quickly form cancer, this model requires the use of three transgenic lines (K14-CreERTAM, LSL-K-ras G12D , p53flox / flox), not only the price is more expensive and the reproduction time is relatively long, but also if this model is used to study the role of Kras in oral cancer, it is difficult to rule out the carcinogenic effect of the joint deletion gene P53

Images