Construction method of Kras mutation related oral mucosa malignant animal model

Abstract

The invention relates to the field of life medicine, and particularly relates to a construction method of a Kras mutation related oral mucosa malignant animal model. The construction method of the mouse oral mucosa malignant animal model comprises the steps that the model is constructed by adopting inducible Kras genetic engineering mice in combination with 4-nitroquinoline 1-oxide (4NQO) chemicalinduction, and on the premise of achieving the cancer formation target, the modeling time is shortened as much as possible. A traditional 4NQO chemical induction oral squamous carcinoma animal modelhas the defect of being long in consumed time (larger than or equal to 20 weeks). According to the method, oral squamous carcinoma is successfully induced by combining Kras mutation with 4NQO, only 12weeks are needed from induction to cancer formation, and compared with a traditional animal model, the time is saved by 2 months for the whole experiment.

Description

technical field [0001] The invention relates to the field of life medicine, in particular to a method for constructing an animal model of oral mucosal malignant transformation related to Kras mutation. Background technique [0002] Ras gene mutations have been reported in about 30% of human tumors, and in human malignant tumors such as pancreatic cancer, colon cancer, lung cancer, gallbladder cancer, and liver cancer, Ras gene mutations are as high as 30% to 90%. After the mutation of Ras gene, the Ras protein is continuously activated, which leads to the continuous proliferation of cells and the occurrence of tumors. The most characteristic proteins of the Ras family mainly include three kinds of H-Ras, K-Ras and N-Ras, and most of the mutations in this family occur in the K-Ras gene. The common mutation sites are codon 12 and codon 13 of exon 2 of K-Ras gene, and codon 61 of exon 3 of K-Ras gene, including the G12D mutation. Currently, Kras G12D There are not many resea...

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Problems solved by technology

Although this method can quickly form cancer, this model requires the use of three transgenic lines (K14-CreERTAM, LSL-K-ras G12D , p53flox / flox), not only the price is more expensive and the reproduction time is relatively long, but also if this model is used to study the role of Kras in oral cancer, it is difficult to rule out the carcinogenic effect of the joint deletion gene P53

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