Construction method of Kras mutation related oral mucosa malignant animal model

A construction method and technology of oral mucosa, applied in the field of life medicine, can solve problems such as relatively expensive, difficult to rule out carcinogenicity, and long time-consuming reproduction

Active Publication Date: 2020-10-23
HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV
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Problems solved by technology

Although this method can quickly form cancer, this model requires the use of three transgenic lines (K14-CreERTAM, LSL-K-ras G12D , p53flox/flox), not only the price is more expensive and t...

Method used

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  • Construction method of Kras mutation related oral mucosa malignant animal model
  • Construction method of Kras mutation related oral mucosa malignant animal model
  • Construction method of Kras mutation related oral mucosa malignant animal model

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Experimental program
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Embodiment 1

[0043] Select 6-8 weeks K14-Cre ERTAM / LSL-K-ras G12D There were 16 mice, including 11 in the experimental group and 5 in the control group.

[0044] (1) Control group: mice were given normal drinking water 1-9 weeks after the start of the experiment, and 1 mg / mouse of tamoxifen (TAM) was injected intraperitoneally at the 9th week, single injection. Afterwards, the mice were fed with normal drinking water for 3 weeks, and the tumors in the lips and cheeks and the tongue tissues of the mice were collected.

[0045] (2) Experimental group: after the start of the experiment, the mice were fed with 4NQO drinking water with a concentration of 100ug / ml in the dark from the 1st to 9th weeks, and tamoxifen (TAM) 1mg / ml was intraperitoneally injected at the end of the 9th week. Only, administered as a single injection. Afterwards, the mice were fed with normal drinking water for 3 weeks, and the tumors in the lips and cheeks and the tongue tissues of the mice were collected.

[004...

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Abstract

The invention relates to the field of life medicine, and particularly relates to a construction method of a Kras mutation related oral mucosa malignant animal model. The construction method of the mouse oral mucosa malignant animal model comprises the steps that the model is constructed by adopting inducible Kras genetic engineering mice in combination with 4-nitroquinoline 1-oxide (4NQO) chemicalinduction, and on the premise of achieving the cancer formation target, the modeling time is shortened as much as possible. A traditional 4NQO chemical induction oral squamous carcinoma animal modelhas the defect of being long in consumed time (larger than or equal to 20 weeks). According to the method, oral squamous carcinoma is successfully induced by combining Kras mutation with 4NQO, only 12weeks are needed from induction to cancer formation, and compared with a traditional animal model, the time is saved by 2 months for the whole experiment.

Description

technical field [0001] The invention relates to the field of life medicine, in particular to a method for constructing an animal model of oral mucosal malignant transformation related to Kras mutation. Background technique [0002] Ras gene mutations have been reported in about 30% of human tumors, and in human malignant tumors such as pancreatic cancer, colon cancer, lung cancer, gallbladder cancer, and liver cancer, Ras gene mutations are as high as 30% to 90%. After the mutation of Ras gene, the Ras protein is continuously activated, which leads to the continuous proliferation of cells and the occurrence of tumors. The most characteristic proteins of the Ras family mainly include three kinds of H-Ras, K-Ras and N-Ras, and most of the mutations in this family occur in the K-Ras gene. The common mutation sites are codon 12 and codon 13 of exon 2 of K-Ras gene, and codon 61 of exon 3 of K-Ras gene, including the G12D mutation. Currently, Kras G12D There are not many resea...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K31/47
CPCA01K67/027A61K31/47A01K2227/105A01K2267/03
Inventor 夏娟程斌邓淼范招纳吴桐陶小安
Owner HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV
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