Non-invasive markers, screening methods and applications of cholangiocarcinoma based on the relative abundance of gut microbes

A technology of gut microbes and relative abundance, applied in the field of non-invasive markers of cholangiocarcinoma, can solve the problem of lack of effective non-invasive diagnostic markers for cholangiocarcinoma, and achieve the effect of improving the accuracy of early diagnosis

Active Publication Date: 2022-06-28
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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Problems solved by technology

Lack of clinically effective non-invasive diagnostic markers for cholangiocarcinoma

Method used

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  • Non-invasive markers, screening methods and applications of cholangiocarcinoma based on the relative abundance of gut microbes
  • Non-invasive markers, screening methods and applications of cholangiocarcinoma based on the relative abundance of gut microbes
  • Non-invasive markers, screening methods and applications of cholangiocarcinoma based on the relative abundance of gut microbes

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Experimental program
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Embodiment Construction

[0027] Specific embodiments of the present invention such as Figure 1-6 As shown, the stool samples of 33 patients with cholangiocarcinoma and 47 subjects without cancer were collected, and 16S rRNA gene detection was performed on the stool samples. The survey records demographic baseline data, including age, sex, weight, height, smoking and drinking habits, etc., and tracks serum tumor markers and liver function indicators. Among the clinical variables, sex, smoking and drinking habits, cirrhosis and history of HBV infection, were comparable between CCA and CF.

[0028] DNA extraction and 16S rRNA gene sequencing:

[0029] First, use the E.Z.N.A. StoolDNAKit (D4015, Omega, Inc., USA) extracted bacterial genomes. Amplifies the V3-V4 region of the prokaryotic (bacterial and archaeal) small subunit (16S) rRNA gene. Throughout the DNA extraction process, ultrapure water was used to rule out the possibility of false positive PCR results as a negative control. PCR products w...

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Abstract

The invention relates to the technical field of genetic engineering, and in particular discloses a non-invasive marker, screening method and application of cholangiocarcinoma based on the relative abundance of intestinal microorganisms, including 12 genera of intestinal flora, the 12 intestinal flora The flora genera were g__Agathobacter, g__Anaerostipes, g__Cetobacterium, g__Clostridium, g__Clostridium_sensu_stricto_1, g__Coprococcus_3, g__Faecalibacterium, g__Intestinimonas, g__Muribaculaceae_unclassified, g__Muribaculum, g__Rothia and g__Turicibacter.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a non-invasive marker, screening method and application of cholangiocarcinoma based on the relative abundance of intestinal microorganisms. Background technique [0002] At present, cholangiocarcinoma accounts for 10%-20% of primary liver cancer, and is the second most common malignant liver tumor. In recent years, the incidence rate has been on the rise worldwide. Cholangiocarcinoma is one of the most aggressive and destructive malignant subtypes. It has the characteristics of insidious onset, rapid growth, easy early metastasis through lymphatic and blood circulation, and poor prognosis. The survival rate of patients with advanced cholangiocarcinoma is less than 5%. Cholangiocarcinoma poses a heavy burden to public health and is a serious global public health problem. [0003] To date, cholangiocarcinoma has been thought to be possibly related to genetic and enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/06G16B40/00
CPCC12Q1/689C12Q1/06G16B40/00G01N2800/7028
Inventor 陈钢李佳靓王怡张斯娜金晨
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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