Biliary duct cancer non-invasive marker based on relative abundance of intestinal microorganisms, screening method and application

A technology of intestinal microbes and relative abundance, applied in the field of non-invasive markers of cholangiocarcinoma, can solve the problem of lack of effective non-invasive diagnostic markers of cholangiocarcinoma, and achieve the effect of improving the accuracy of early diagnosis

Active Publication Date: 2021-03-26
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

Lack of clinically effective non-invasive diagnostic markers for cholangiocarcinoma

Method used

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  • Biliary duct cancer non-invasive marker based on relative abundance of intestinal microorganisms, screening method and application
  • Biliary duct cancer non-invasive marker based on relative abundance of intestinal microorganisms, screening method and application
  • Biliary duct cancer non-invasive marker based on relative abundance of intestinal microorganisms, screening method and application

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Embodiment Construction

[0027] Specific implementation of the present invention, for example Figure 1-6 As shown, 33 feces samples of 33 and non-cancer 47 subjects were collected, and 16S rRNA gene was tested for 16S rRNA gene. Investigation records of demographics baseline data, including age, gender, weight, height, smoking and drinking habits, etc., trace serum tumor markers, liver function indicators. In clinical variables, gender, smoking and drinking habits, cirrhosis and HBV infection, comparable between CCA and CF.

[0028] DNA extraction and 16S rRNA sequencing:

[0029] First, use E.z.n.a. StoolDnakit (D4015, Omega, Inc., USA) extracts the bacterial genome. Amplifies the V3-V4 region of the prokaryic (bacteria and ancient bacteria) subunit (16S) rRNA gene. During the entire DNA extraction, ultrapure water is used to exclude the likelihood of false positive polymerase chain reaction as a negative control. The polymerase chain reaction product was purified with Ampure XT BEADS (Beckman Coulter G...

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Abstract

The invention relates to the technical field of gene engineering, particiculary discloses a biliary duct cancer non-invasive marker based on relative abundance of intestinal microorganisms, screeningmethod and application. The biliary duct cancer non-invasive marker comprises 12intestinal flora, wherein the 12 intestinal flora are g-Agathobacter, g-Anaerostipes, g-Cetobacterium, g-Clostridium, g-Clostridium-sensu_stricto-1, g-Coprococcus-3, g-Faecalibacterium, g-Intestinimonas, g-Muribaculaceae-unclassified, g-Muribaculum, g-Rothia and g-Turicibacter.

Description

Technical field [0001] The present invention relates to the field of genetic engineering technology, in particular, a non-invasive marker, screening method, and application based on intestinal microorganism. Background technique [0002] At present, cholangiocarcinoma accounts for 10% -20% of primary liver cancer, which is the second common malignant liver tumor. In recent years, the incidence rate in the world has been rising. Choloreal cancer is one of the most invasive and destructive malignant subtypes, with hidden growth, rapid growth, easy to transfer early transition, prenose, adverse prognosis, late cholangiocarcinoma patient survival is less than 5%. Bile duct cancer brings a heavy burden to the public, it is a serious global public health problem. [0003] To date, biliary tube cancer is considered to be related to genetic and environmental factors, and its specific pathology is still unable to explain. The study found that the human intestinal tract has a wide variety ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/06G16B40/00
CPCC12Q1/689C12Q1/06G16B40/00G01N2800/7028
Inventor 陈钢李佳靓王怡张斯娜金晨
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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