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Gene chips, molecular probe combinations, kits and applications for analyzing whether sheep have horns

A technology of molecular probe and gene chip, which is applied in the field of gene chip for analyzing whether sheep have horns, can solve the problems of insufficient expression of functional sites and regions, insufficient uniformity of sites, etc., and achieve shortened generation interval, low cost, The effect of small flux

Active Publication Date: 2021-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the existing sheep SNP chip is mainly based on the data of western sheep breeds, and lacks the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, there are still insufficient site uniformity, insufficient reflection of functional sites and regions, and more than 10 % of the loci are very low frequency loci in Chinese sheep populations and many other problems, so it is of great significance to design a SNP chip that is suitable for Chinese sheep populations and can quickly and effectively detect whether sheep have horns

Method used

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  • Gene chips, molecular probe combinations, kits and applications for analyzing whether sheep have horns
  • Gene chips, molecular probe combinations, kits and applications for analyzing whether sheep have horns
  • Gene chips, molecular probe combinations, kits and applications for analyzing whether sheep have horns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Obtaining whether there is a combination of SNP loci for angular traits

[0030] 1. Selection of individual sheep

[0031]In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the present invention collects the genetic information of 248 sheep individuals all over Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Zeller black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Y...

Embodiment 2

[0044] Embodiment 2 is used to judge whether the combination of SNP sites with angles is used to prepare primer combinations and probe combinations

[0045] Those skilled in the art design primers according to the sequence information of each site in the site combination provided by the present invention, and evaluate the secondary structure and Tm value of the designed primers to finally obtain good specificity and high sensitivity. And the primers for the purpose of detection can be realized under the same reaction conditions.

[0046] Wherein, the evaluation of the secondary structure and the Tm value can be carried out by any method commonly used in the art, for example, the evaluation of the secondary structure by DNA folding form, see ( ), and then use the software RaW-Probe to evaluate its Tm value.

[0047] The above methods are conventional methods. According to the site information in the combination of angled SNP sites provided by this application, it can be obtai...

Embodiment 3

[0049] Example 3 Combining SNP sites for the preparation of gene chips

[0050] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.

[0051] It should be noted that those skilled in the art can use any method to prepare a SNP gene chip for detecting whether a sheep has horns, or entrust a biological company to prepare it, but the SNP gene chip prepared based on the combination of whether there are horns or not provided by this application All SNP gene chips belong to the protection scope of the present invention.

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Abstract

The invention discloses a gene chip, molecular probe combination, kit and application for analyzing whether sheep have horns, and relates to the field of biotechnology. The invention provides 514 SNP sites that reflect whether sheep have horns as shown in Table 1 Combinations and molecular probe combinations, gene chips, and kits formed based on the combination of sites can be used to carry out genetic evaluation of individual sheep by using the site combinations, molecular probe combinations, gene chips, and kits provided by the present invention. Early detection of whether a sheep individual has horns and evaluation of its genetic information can also detect sheep phenotypes early in the breeding process, control the breeding process, and save breeding costs. It can also be applied to sheep pedigree reconstruction, germplasm resource protection, Improvement, variety identification and selection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of biodetection technology, and more specifically to a gene chip for analyzing whether a sheep has horns, a molecular probe combination, a kit and an application thereof. Background technique [0002] Sheep are one of the most economically important animals in the world. Among the many sheep breeds, some sheep have no horns, and some sheep have horns, but with the development of animal husbandry production and changes in market demand, some sheep breeds have begun to disappear or are in a state of danger, and because sheep with big horns are aggressive , often destroy facilities and fight with each other, causing farmers to be reluctant to raise ewes with big horns. Therefore, it is imminent to study whether sheep have horns. It will be determined between half a year old and one year old. Therefore, the indirect determination of polyhedral phenotypes by means of DNA-based...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner CHINA AGRI UNIV
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