Gene Chip, Molecular Probe Combination, Kit and Application for Analyzing Sheep Wool Color

A technology of molecular probe and gene chip, which is applied in the field of gene chip for analyzing sheep wool color, can solve the problems of insufficient uniformity of sites, insufficient expression of functional sites and regions, etc., and achieve low cost, saving breeding costs, and universality Good results

Active Publication Date: 2021-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sheep SNP chips are mainly based on the data of western breeds of sheep, lacking the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, and there are insufficient site uniformity, insufficient reflection of functional sites and regions, and more than 10% There are many problems such as extremely low-frequency loci in Chinese sheep populations. It is of great significance to design a SNP chip suitable for Chinese sheep populations that can quickly and effectively detect coat color.

Method used

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  • Gene Chip, Molecular Probe Combination, Kit and Application for Analyzing Sheep Wool Color
  • Gene Chip, Molecular Probe Combination, Kit and Application for Analyzing Sheep Wool Color
  • Gene Chip, Molecular Probe Combination, Kit and Application for Analyzing Sheep Wool Color

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Obtaining the Combination of Coat Color Trait SNP Loci

[0039] 1. Selection of individual sheep

[0040]In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the applicant collected genetic information from 248 sheep individuals in Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Celler black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Yankasa Sheep in Nigeria, West Af...

Embodiment 2

[0053] Example 2 Combination of hair color SNP sites for preparation of primer combination and probe combination

[0054] Those skilled in the art design primers according to the sequence information of each site in the fur color SNP site combination provided by the present invention, and perform secondary structure evaluation and Tm value evaluation on the designed primers to finally obtain good specificity and sensitivity. Primers that are high and can achieve detection purposes under the same reaction conditions.

[0055] Wherein, the evaluation of secondary structure and Tm value can be carried out by any method commonly used in the art, for example, using DNA folding form to evaluate its secondary structure, see (http: / / unafold.rna.albany.edu / ? q=mfold / DNA-Folding-Form), and then use the software RaW-Probe to evaluate its Tm value.

[0056] The above methods are conventional methods. According to the site information in the fur color SNP locus combination provided by thi...

Embodiment 3

[0058] Example 3 will analyze sheep wool color SNP site combination for the preparation of gene chips

[0059] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.

[0060] It should be noted that those skilled in the art can use any method to prepare the SNP gene chip for detecting sheep wool color, and can also entrust a biological company to prepare it, but the SNP gene chip prepared based on the combination of wool color SNP loci provided by this application is all Belong to the protection scope of the present inven...

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Abstract

The invention discloses a gene chip, molecular probe combination, kit and application for analyzing wool color of sheep, and relates to the field of biotechnology. The invention provides a combination of 1941 SNP sites for analyzing sheep wool color traits at the genetic level, and its physical location The information is determined based on the comparison of the sheep v4.0 genome sequence. The combination of molecular probes, gene chips, and kits obtained by using the 1941 SNP sites provided by the present invention can carry out genetic evaluation on the coat color traits of sheep. It can carry out individual selection, shorten the generation interval, speed up the breeding process, save breeding costs, realize the screening and identification of varieties based on sheep wool color, and help the reconstruction of sheep pedigree, the protection and improvement of germplasm resources.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of biodetection technology, and more specifically to a gene chip for analyzing wool color of sheep, a molecular probe combination, a kit and its application. Background technique [0002] There are many kinds of sheep coat colors, and their genetic phenomena are very complicated. At the same time, the coat color of sheep has important economic significance. For example, the price of white cashmere is higher than that of other colors. Moreover, the coat color of some varieties plays an important role in adapting to the natural environment. In the breeding work of sheep, the coat color trait is an important breed characteristic and production trait, which is of great significance in determining the hybrid combination, breed purity and kinship, especially in obtaining the required coat color type through cross breeding In addition, sheep wool color is also of great significanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner CHINA AGRI UNIV
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