Gene chips, molecular probe combinations, kits and applications for the analysis of suet tail
A technology of molecular probe and gene chip, which is applied in the field of molecular probe combination for the analysis of suet tail, can solve the problems of insufficient expression of functional sites and regions, insufficient uniformity of sites, etc., and achieve breeding cost saving and low cost , universal effect
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Embodiment 1
[0044] Example 1 Obtaining the Combination of SNP Sites for Fatty Tail Characters
[0045] 1. Selection of individual sheep
[0046] In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the applicant collected genetic information from 248 sheep individuals in Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Celler black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Yankasa Sheep in Niger...
Embodiment 2
[0059] Example 2 Use the combination of lipid-tailed SNP sites to prepare primer combinations and probe combinations. Those skilled in the art design primers according to the sequence information of each site in the combination of lipid-tailed SNP sites provided by the present invention, and design The primers were evaluated for secondary structure and Tm value, and finally obtained primers with good specificity, high sensitivity, and detection purposes under the same reaction conditions.
[0060] Wherein, the evaluation of secondary structure and Tm value can be carried out by any method commonly used in this field, for example, using DNA folding form to evaluate its secondary structure, see (http: / / unafold.ma.albany.edu / ? =mfold / DNA-Folding-Form), and then use the software RaW-Probe to evaluate its Tm value.
[0061] The above methods are conventional methods. According to the site information in the combination of lipid-tailed SNP sites provided by this application, it can ...
Embodiment 3
[0063] Example 3 Combination of Lipid Tail SNP Sites for Preparation of Gene Chips
[0064] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.
[0065] It should be noted that those skilled in the art can use any method to prepare the SNP gene chip for detecting sheep fat tail, and can also entrust a biological company to prepare it, but the SNP gene prepared based on the combination of fat tail SNP sites provided by this application Chips all belong to the protection scope of the present invention.
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