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Gene chips, molecular probe combinations, kits and applications for the analysis of suet tail

A technology of molecular probe and gene chip, which is applied in the field of molecular probe combination for the analysis of suet tail, can solve the problems of insufficient expression of functional sites and regions, insufficient uniformity of sites, etc., and achieve breeding cost saving and low cost , universal effect

Active Publication Date: 2021-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sheep SNP chip is mainly based on the data of western sheep breeds, lacks the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, and has insufficient locus uniformity, insufficient reflection of functional loci and regions, and more than 10% There are many problems such as extremely low-frequency loci in Chinese sheep populations. It is of great significance to design a SNP chip suitable for Chinese sheep populations that can quickly and effectively detect lipid tails.

Method used

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  • Gene chips, molecular probe combinations, kits and applications for the analysis of suet tail
  • Gene chips, molecular probe combinations, kits and applications for the analysis of suet tail
  • Gene chips, molecular probe combinations, kits and applications for the analysis of suet tail

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Obtaining the Combination of SNP Sites for Fatty Tail Characters

[0045] 1. Selection of individual sheep

[0046] In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the applicant collected genetic information from 248 sheep individuals in Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Celler black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Yankasa Sheep in Niger...

Embodiment 2

[0059] Example 2 Use the combination of lipid-tailed SNP sites to prepare primer combinations and probe combinations. Those skilled in the art design primers according to the sequence information of each site in the combination of lipid-tailed SNP sites provided by the present invention, and design The primers were evaluated for secondary structure and Tm value, and finally obtained primers with good specificity, high sensitivity, and detection purposes under the same reaction conditions.

[0060] Wherein, the evaluation of secondary structure and Tm value can be carried out by any method commonly used in this field, for example, using DNA folding form to evaluate its secondary structure, see (http: / / unafold.ma.albany.edu / ? =mfold / DNA-Folding-Form), and then use the software RaW-Probe to evaluate its Tm value.

[0061] The above methods are conventional methods. According to the site information in the combination of lipid-tailed SNP sites provided by this application, it can ...

Embodiment 3

[0063] Example 3 Combination of Lipid Tail SNP Sites for Preparation of Gene Chips

[0064] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.

[0065] It should be noted that those skilled in the art can use any method to prepare the SNP gene chip for detecting sheep fat tail, and can also entrust a biological company to prepare it, but the SNP gene prepared based on the combination of fat tail SNP sites provided by this application Chips all belong to the protection scope of the present invention.

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Abstract

The invention discloses a gene chip for analyzing sheep fat tail, a combination of molecular probes, a kit and its application, and relates to the field of biotechnology. The invention firstly provides 3000 combinations of SNP sites for analyzing the traits of sheep fat tail, and the sites The combinations are shown in Table 1; and biological products such as molecular probe combinations, gene chips, and kits prepared based on the above-mentioned site combinations; using the site combinations and related biological products provided by the present invention, sheep individuals can be tested from the genetic level. Genetic evaluation, variety screening and identification, individual selection of sheep fat tail traits can be carried out in the early difficult-to-measure stage, control the breeding process, save breeding costs, and can also be used for sheep breed traceability, sheep pedigree reconstruction, and germplasm resource protection and germplasm resource improvement.

Description

technical field [0001] The present invention relates to the field of biological technology, specifically to the technical field of biological detection, and more specifically to biological products such as molecular probe combinations, gene chips, and kits for analyzing suet tail, and their applications. Background technique [0002] Sheep tail fat can be used as a form of energy storage, which can provide energy for sheep to migrate or survive the severe winter of lack of forage, so as to maintain their survival needs; on the other hand, it can be used as a high-energy food for human use. However, with the continuous improvement of sheep breeding conditions in pastoral or agricultural areas or the continuous improvement of human living standards, the effect of sheep tail fat on sheep itself or human beings gradually becomes smaller, and people's demand for high-fat mutton decreases. And some studies have shown that the feed required by livestock to deposit 1kg of fat is equ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6837C12N15/11C40B40/06
CPCC12Q1/6888C12Q1/6837C40B40/06C12Q2600/156C12Q2600/124
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner CHINA AGRI UNIV
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