A cell culture automatic operation device

Abstract

The present invention relates to an automatic cell culture operation device, including a mechanical arm module, a clamping and moving module, a cover opening module, a consumable storage module, a liquid processing module, a solid processing module, and a waste liquid processing module; the cover opening module includes a thawing cover opening mechanism and a cover opening and closing mechanism; under the instruction of the control system, the mechanical arm module cooperates with the consumable storage module, the cover opening module and the transmission module through the clamping and moving module, and cooperates with the liquid processing module to complete the automatic culture of cells, and solid or liquid wastes generated during the operation are recovered by the corresponding solid processing module and the waste liquid processing module. The technical solution of the present invention switches the clamping and moving module through the mechanical arm module, and with the cooperation of other functional modules, it well simulates the various operations manually performed by the experimenter, can operate continuously and uninterruptedly, has high efficiency, reduces the dependence on the operation level and experience of the personnel, and reduces the differences in cell products due to different batches.

Description

Technical Field

[0001] The invention relates to the field of biological tissue production, in particular to an automatic operation device for cell culture. Background Art

[0002] In related technologies, cell culture is mostly done manually or semi-automatically with the help of equipment. This biological cell production method that relies heavily on manual labor is inefficient and more dependent on the operator's operating level and experience. There are large differences in cell products produced by different operators or different batches of the same operator. Summary of the invention

[0003] The technical problem to be solved by the present invention is to overcome the defects of low production efficiency and reliance on operator experience in the prior art, and further provide an automatic cell culture operation device, which simulates the manual operation process of the experimenter through an automated device to ensure that the operation process is continuous, stable, uninterrupted and the operation efficiency is high.

[0004] The cell culture automatic operation equipment includes a robotic arm module, a clamping and moving module, a clamping and moving installation module, a cover opening module, a consumable storage module, a liquid processing module, a solid processing module, and a waste liquid processing module; under the instruction of the control system, the robotic arm module cooperates with the consumable storage module, the cover opening module and the transmission module through the clamping and moving module, and cooperates with the liquid processing module to complete the automatic cell culture, and the solid or liquid waste generated during the operation is recovered by the corresponding solid processing module and the waste liquid processing module.

[0005] Wherein, the cover opening module includes a thawing cover opening mechanism and a cover opening and closing mechanism;

[0006] The robotic arm module cooperates with the first switching piece on the robotic arm module and the second switching piece on the clamping and moving module to realize the operation of the robotic arm module on the clamping and moving module; the clamping and moving module includes a batch grabbing mechanism, a clamping mechanism and a pipetting mechanism, wherein the batch grabbing mechanism can grab the freezing components used for freezing cell samples, the clamping mechanism can operate culture bottles, centrifuge tubes, etc., and the pipetting mechanism can be used for operations such as aspirating or spitting liquid.

[0007] Furthermore, the thawing and opening mechanism cooperates with the batch grabbing mechanism to complete the thawing operation on the frozen storage component, and the clamping mechanism, the pipetting mechanism and the centrifuge cooperate with each other to complete the preparation of the suspended cell fluid of the frozen storage component. After the suspended cell fluid is packaged, reagents are added for culture, and the growth status of the cells is observed in real time by a microscope during the culture process.

[0008] Furthermore, the batch grabbing mechanism includes a base shell, a grabbing piece hinged on the base shell, and a plurality of tube grabbing pieces installed on the bottom surface of the base shell, the side of the grabbing piece away from the base shell is clamped on the outer surface of the freezing shell of the freezing assembly, a plurality of freezing tubes are installed in the freezing shell at intervals, and the side of each of the tube grabbing pieces away from the base shell is inserted and fixed in each of the freezing tubes in a one-to-one correspondence; the batch grabbing mechanism also includes a cylinder arranged on the side of the base shell away from the tube grabbing piece, a moving piece fixedly connected to the output end of the cylinder and installed in the base shell, the moving piece and the grabbing piece being arranged to slide relative to each other; the batch grabbing mechanism also includes a guide block fixedly arranged on the side of the grabbing piece close to the moving piece, and a guide shaft slidably arranged on the guide block, the side of the guide shaft away from the guide block is fixed to the grabbing piece.

[0009] Furthermore, the clamping mechanism includes a first driving member, and a plurality of clamping groups fixed to the first driving member, each of the clamping groups is clamped on the outer surface of the centrifuge tube or the culture storage mechanism, and each of the clamping groups includes a plurality of clamping members arranged in a surrounding manner along the side of the first driving member.

[0010] Furthermore, the above-mentioned consumable storage module includes a carrier for carrying pipette tips, and at least one positioning frame installed on the carrier, each of the positioning frames includes a positioning seat fixed on the carrier, a pressure piece rotatably arranged on the positioning seat, and the pressure piece abuts against the consumable shell used to mount the centrifuge tube.

[0011] Furthermore, the above-mentioned clamping and moving installation module includes a mounting frame installed in the shell module, and a mounting piece arranged on the mounting frame. The clamping and moving module is stored on the clamping and moving installation module through the snap-fit ​​cooperation between the mounting piece and the positioning piece on the clamping and moving module.

[0012] Furthermore, the above-mentioned liquid processing module is used to transfer the reagent to the culture bottle or the centrifuge tube, and it includes a pipette line, a heating component and a pump body installed on the pipette line, and a sample adding component connected to one end of the pipette line, and the other end of the pipette line is connected to the culture bottle or the centrifuge tube.

[0013] Furthermore, the solid processing module is used to recycle discarded culture carriers or centrifuge tubes or pipette tips and other waste; the waste liquid processing module is used to process waste such as liquids poured from the centrifuge tubes or culture bottles; the conveying module is used to transport culture samples in and out of the shell module; the opening and closing cover mechanism is used for the opening and closing operations of the covers of culture bottles or centrifuge tubes; the environmental control module is used to maintain the environmental conditions required during the cell culture operation; and each of the above modules is installed in the shell module.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The above technical solution provides an automatic cell culture operation device, which switches various types of clamping and moving mechanisms through a mechanical arm and cooperates with various functional modules to simulate various experimental operations performed manually by experimenters. Compared with pure manual operation, the automatic operation process of the machine is more stable, more continuous, uninterrupted, and has higher operating efficiency, which reduces the dependence on the operation level and experience of personnel and reduces the differences in cell products due to different batches. The dependence on the operation level and experience of personnel reduces the differences in cell products due to different batches. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 This is a schematic diagram of the internal structure of an automatic cell culture operation device according to an embodiment of the present invention;

[0018] Figure 2 for Figure 1 A partial schematic diagram of a robotic arm module from one viewing angle is shown;

[0019] Figure 3 It is a structural schematic diagram of a batch grabbing mechanism and a freezing component in an embodiment of the present invention;

[0020] Figure 4 It is a structural schematic diagram of a batch grabbing mechanism and a cryopreservation tube in an embodiment of the present invention;

[0021] Figure 5 It is a schematic diagram of the structure of the clamping mechanism and the culture bottle in the embodiment of the present invention;

[0022] Figure 6 An exploded schematic diagram of a clamping mechanism, a cryopreservation tube, and a centrifuge tube in an embodiment of the present invention;

[0023] Figure 7 A schematic diagram of the structure of a consumable storage module in an embodiment of the present invention;

[0024] Figure 8 for Figure 1 The structural schematic diagram of the pipetting mechanism shown;

[0025] Fig. 9 This is a structural schematic diagram of a liquid processing module from one viewing angle in an embodiment of the present invention;

[0026] Fig.10 for Figure 1 A schematic structural diagram of the clamping and moving installation module from one viewing angle is shown;

[0027] Description of reference numerals:

[0028] 100, housing module; 10, centrifuge tube; 20, consumable shell; 30, pipette tip; 40, refrigerator; 50, centrifuge;

[0029] 211. Cryopreservation shell; 212. Cryopreservation tube; 220. Culture bottle;

[0030] 300, robot arm module; 310, first switching component; 320, second switching component;

[0031] 400, clamping and moving module; 410, batch grabbing mechanism; 411, base shell; 412, grabbing member; 413, pipe grabbing member; 414, cylinder; 420, clamping mechanism; 421, first driving member; 422, clamping group; 423, clamping member; 430, liquid transfer mechanism;

[0032] 500. Thawing and opening mechanism;

[0033] 600, consumable storage module; 610, carrier; 620, positioning frame;

[0034] 700, solid handling module;

[0035] 800, transmission module;

[0036] 900, cover opening and closing mechanism;

[0037] 1000, liquid handling module; 1001, pipetting pipeline; 1002, pump body; 1100, heating component;

[0038] 2000, waste liquid treatment module;

[0039] 3000, clamping and moving installation module; 3001, installation frame; 3002, installation piece; 3003, positioning piece;

[0040] 4000, environmental control module;

[0041] 5000. Microscope. DETAILED DESCRIPTION

[0042] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0044] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] See also Figure 1-10 The embodiment of the present invention provides an automatic cell culture operation device, which has high operating efficiency, reduces dependence on personnel's operating level and experience, and reduces the differences in cell products due to different batches.

[0046] The cell culture automatic operation device at least includes: a housing module 100; a culture storage mechanism for storing cells, including a cryopreservation assembly, a culture bottle, or a centrifuge tube; a robot module 300 and a clamping module 400, wherein the robot module 300 is installed in the housing module 100, and the clamping module 400 includes a batch grabbing mechanism 410 and a clamping mechanism 420. The batch grabbing mechanism 410 and / or the clamping mechanism 420 are installed on the side of the robot module 300 away from the housing module 100, and the batch grabbing mechanism 410 can clamp the cryopreservation assembly Components; a consumable storage module 600, the consumable storage module 600 includes a carrier 610 installed in the housing module 100 and used to place the centrifuge tube 10; wherein the robotic arm module 300 carries the batch grasping mechanism 410 to clamp the cryopreservation component and moves to the cryopreservation tube thawing and opening mechanism 500 to thaw the cryopreservation component, the clamping mechanism 420 moves the centrifuge tube 10 to the target position, the cell fluid in the cryopreservation component is transferred to the centrifuge tube 10 and processed to obtain a suspended cell fluid, and the suspended cell fluid is cultured after being packaged and filled with reagents.

[0047] It should be noted that the batch grabbing mechanism 410 and the clamping mechanism 420 can be installed on one end of the robot arm module 300 at the same time, or only the batch grabbing mechanism 410 or the clamping mechanism 420 can be installed; the clamping mechanism 420 can be used to clamp the centrifuge tube 10, and of course, can also clamp other components in the cell culture process such as the freezing component; the culture storage mechanism can be transmitted from the outside to the inside of the shell module 100 during the production process, and can also be set inside the shell module 100 before production.

[0048] It can be understood that the joint action of the robot arm module 300 and the clamping and moving module 400 can better replace manual operation to improve work efficiency. The thawing and opening mechanism 500 thaws and opens the frozen storage components, and the clamping mechanism 420 uses the carrier 610 to store the required centrifuge tubes 10. In conjunction with multiple action processes of other modules, cell culture operations are performed in the same shell module 100, which reduces the participation of personnel in the cell culture process, can better eliminate the uncertainty caused by human operation, reduce the dependence on personnel operation level and experience in production, and reduce the differences in cell products due to different batches.

[0049] The cell culture automatic operation device further includes a first switching member 310 fixedly arranged on an end of the robot arm module 300 away from the housing module 100, and a second switching member 320 locked with the first switching member 310, and a side of the second switching member 320 away from the first switching member 310 is fixedly arranged on the clamping module 400. It can be understood that Figure 2-3 As shown, the first switching member 310 and the second switching member 320 can realize the rapid connection or separation of the robot arm module 300 and the clamping and moving module 400.

[0050] In order to realize the clamping effect of the batch grabbing mechanism 410 on the freezing storage component, the freezing storage component includes a freezing storage shell 211 and a freezing tube 212 installed in the freezing storage shell 211. The batch grabbing mechanism 410 includes a base shell 411, a grabbing piece 412 hinged on the base shell 411, and a tube grabbing piece 413 installed on the bottom surface of the base shell 411. The side of the grabbing piece 412 away from the base shell 411 is clamped on the outer surface of the freezing storage shell 211, and the side of the tube grabbing piece 413 away from the base shell 411 is inserted and fixed in the freezing tube 212.

[0051] It is understood that the cryopreservation tube 212 contains cells in a frozen state, and the cryopreservation shell 211 can store one or more cryopreservation tubes 212, such as Figure 3-4As shown, the grasping piece 412 is hinged to the base shell 411 through a hinge axis, and the grasping piece 412 can rotate relative to the base shell 411, which can not only clamp or release the freezing shell 211, but also adapt to clamp freezing shells 211 of different sizes, and has good applicability. Under the action of the robotic arm module 300, the tube grasping piece 413 and the freezing tube 212 are fixed by interference fit, and the freezing tube 212 fixed on the tube grasping piece 413 is transferred to the thawing and opening mechanism 500.

[0052] In one embodiment, the movable part is a plate, and the pushing part can be a push rod. The number and position of the tube grabbing parts 413 correspond to the number and position of the freezing tubes 212. The number of the tube grabbing parts 413 and the pushing parts are the same. The cylinder 414 is used to drive the movable part to move up and down relative to the base shell 411. The pushing part can move axially along the tube grabbing part 413 with the movable part. When the freezing tube 212 needs to be removed from the tube grabbing part 413, the cylinder 414 drives the movable part to move downward, and the movable part drives the pushing part to move downward relative to the tube grabbing part 413 to push the freezing tube 212 away from the pushing part. The movable member 415 moves up and down with the guide shaft, and an inclined guide groove is formed on the guide block. When the guide shaft slides in the guide groove, one side of the grasping member 412 is hinged to rotate relative to the base shell 411, and the other side moves in the direction of approaching or moving away from the movable member, so that the grasping member 412 opens or closes, thereby realizing the clamping or release of the frozen storage shell 211 by the batch grasping mechanism 410.

[0053] In order to achieve the clamping effect of the clamping mechanism 420, as Figure 5-6 As shown, the clamping mechanism 420 includes a first driving member 421, and a plurality of clamping groups 422 fixed on the first driving member 421, each clamping group 422 includes two clamping members 423, wherein one clamping group 422 is used to clamp the culture bottle 220, another clamping group 422 is used to clamp the cryopreservation tube 212, and another clamping group 422 is used to clamp the centrifuge tube 10. Of course, the number, size and shape of the clamping groups 422 can be adjusted as needed. The first driving member 421 can be a cylinder 414, and the first driving member 421 drives the clamping group 422 so that the two clamping members 423 in the clamping group 422 move toward or away from each other to realize the opening or closing of the clamping group 422.

[0054] In order to install the centrifuge tube 10, the consumable storage module 600 also includes at least one positioning frame 620 installed on the carrier 610, each of the positioning frames 620 includes a positioning seat fixed on the carrier 610, and a pressing piece rotatably arranged on the positioning seat, and the pressing piece abuts against the consumable shell 20 for installing the centrifuge tube 10. It can be understood that the carrier 610 is a temporary storage point for various consumables to be taken, and the consumables can be a pipette tip 30, a centrifuge tube 10, a disposable cell counting plate, etc. In this embodiment, Figure 7 As shown, there are multiple positioning racks 620, each of which can hold a corresponding consumable, which can be used to meet the needs of setting up multiple consumables. The pressing piece rotates around a rotating axis to press the consumable shell 20, so that the consumable shell 20 can be stably set on the positioning seat, thereby allowing the centrifuge tube 10 to be stably set on the carrier 610.

[0055] In one embodiment, the positioning frame 620 also includes a positioning switch disposed on the positioning seat and used to detect whether the consumable shell 20 is placed in place. When the consumable shell 20 moves to the positioning seat, if the consumable shell 20 touches the positioning switch, the consumable shell 20 is placed in place.

[0056] In one embodiment, if Figure 8 As shown, the cell culture automatic operation equipment also includes a pipette tip 30 installed on the carrier 610 and used to transfer liquid. The pipette tip 30 is used by the pipette mechanism 430 on the clamping module 400 to realize the function of aspirating or spitting liquid. The specifications of the pipette tip 30 can refer to the existing technology, such as 10ul, 20ul, 500ul, 1ml, 10ml, etc., which can be used to transfer liquids in the cell culture process such as cell fluid in the freezing tube 212 and the centrifuge tube 10. The pipette mechanism 430 and the pipette tip 30 are set to further reduce the participation of personnel in cell production. The aspirated and spitted liquids are measured by the pipette tip 30 of specific specifications, which improves the accuracy of liquid transfer and thus improves the quality of cell products.

[0057] In one embodiment, the cell culture automatic operation equipment further includes a solid processing module 700 for recycling the discarded culture storage mechanism or the centrifuge tube 10 or the pipette tip 30 .

[0058] In one embodiment, the cell culture automatic operation device further includes a conveying module 800 for transporting the culture storage mechanism into and out of the housing module 100. It should be noted that the conveying module 800 is used to transport the cell strain to be amplified into and out of the cell culture automatic operation device. The cell strain to be amplified may be an adherent cell inoculated into a culture bottle 220 or a cell in a frozen state in a cryopreservation tube 212. Therefore, the conveying module 800 may transport a culture storage mechanism such as a culture bottle 220 or a cryopreservation tube 212.

[0059] In one embodiment, the cell culture automatic operation device further includes a cover opening and closing mechanism 900 for opening and closing the cover of the centrifuge tube 10 or the culture storage mechanism.

[0060] In one embodiment, if Fig. 9 As shown, the culture storage mechanism also includes a culture bottle 220 inoculated with cells, and the cell culture automatic operation equipment also includes a liquid processing module 1000 for transferring reagents to the culture bottle 220 or the centrifuge tube 10, and the liquid processing module includes a pipette line 1001, a heating component 1100 and a pump body 1002 both installed on the pipette line 1001, and a sample adding component connected to one end of the pipette line 1001, and the other end of the pipette line 1001 is connected to the culture bottle 220 or the centrifuge tube 10.

[0061] It should be noted that the liquid processing module 1000 is used to heat the biological reagents stored in the refrigerator 40 or in other locations before entering the centrifuge tube 10 or the culture storage mechanism, and the heating component 1100 is used to restore the liquid to room temperature. The heating method of the heating component 1100 includes but is not limited to water bath heating, oil bath heating, metal bath heating, hot air flow heating, etc. The number of the pipette line 1001 and its adapted pump body 1002 and the heating component 1100 corresponds to each other, and can be any number not less than one.

[0062] In one embodiment, the cell culture automatic operation equipment further includes a waste liquid treatment module 2000 for processing the dumping of the centrifuge tube 10 and / or the culture bottle 220 .

[0063] The cell culture automatic operation equipment also includes a clamping and moving installation module 3000 for placing the clamping and moving module 400 to be used, and the clamping and moving installation module 3000 includes a mounting frame 3001 installed in the shell module 100, a mounting member 3002 arranged on the mounting frame 3001, and a positioning member 3003 that is snap-fitted with the mounting member 3002, and the positioning member 3003 is fixedly arranged on the clamping and moving module 400.

[0064] It is understandable that if Fig.10 As shown, in this embodiment, the mounting member 3002 is U-shaped, and the two sides of the mounting member 3002 can be elastically deformed outward under the action of external force and reset after the external force is removed. The mounting member 3002 is fixedly connected to the mounting frame 3001 by bolts. When it is necessary to fix the clamping module 400 on the mounting member 3002, the positioning member 3003 on the clamping module 400 is moved close to the mounting member 3002, so that the positioning member 3003 and the mounting member 3002 cooperate with each other, and the positioning member 3003 is moved along the mounting member 3002. The opening direction of the component 3002 moves inward, and at the same time, the positioning component 3003 squeezes the two sides of the mounting component 3002, and the positioning component 3003 moves to the innermost side of the mounting component 3002, so that the positioning component 3003 is locked by the mounting component 3002, thereby realizing the installation of the clamping and moving module 400 to be used. The clamping and moving mounting module 3000 is set so that the clamping and moving module 400 can be obtained at any time when it is needed, which better ensures the continuity of cell culture, improves work efficiency, and reduces the participation of personnel in the cell culture process.

[0065] In one embodiment, the cell culture automatic operation device further includes an environment control module 4000. It is understandable that the environment control module 4000 can regulate the working environment inside the housing module 100 in real time.

[0066] In one embodiment, the cell culture automatic operation equipment also includes a centrifuge 50 for separating suspended particles and a refrigerator 40 for storing experimental reagents. The centrifuge 50 is used for separating and extracting cells from a cell suspension, or for separating substances of different densities. The refrigerator 40 refrigerates and stores biological reagents, which can better ensure the consistency of automated production and reduce production errors.

[0067] In one embodiment, the cell culture automatic operation equipment further includes a microscope 5000 for observing the cells growing in the culture bottle.

[0068] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A cell culture automatic operation device, characterized in that: The invention comprises a mechanical arm module (300), a clamping and moving module (400), a clamping and moving installation module (3000), a conveying module (800), a cover opening module, a consumable material storage module (600), a liquid processing module (1000), a solid processing module (700), and a waste liquid processing module (2000); wherein the cover opening module comprises a thawing and opening cover mechanism (500) and a cover opening and closing mechanism (900); the mechanical arm module (300) realizes the operation of the clamping and moving module (400) by the mechanical arm module (300) through the cooperation of a first switching member (310) on the mechanical arm module and a second switching member (320) on the clamping and moving module; the clamping and moving module (400) comprises a batch The invention relates to a batch grabbing mechanism (410), a clamping mechanism (420) and a liquid transfer mechanism (430), wherein the batch grabbing mechanism (410) grabs a freezing component for freezing a cell sample, and the clamping mechanism (420) is used to operate a culture bottle (220) and a centrifuge tube (10); under the instruction of the control system, the robot arm module (300) cooperates with the consumable storage module (600), the cover opening module and the transmission module (800) through the clamping and transfer module (400), and cooperates with the liquid processing module (1000) to complete the automatic cell culture, and the waste generated during the operation is recovered by the corresponding solid processing module (700) and the waste liquid processing module (2000); The batch grabbing mechanism (410) includes a base shell (411), a grabbing member (412) hinged on the base shell (411), and a plurality of tube grabbing members (413) installed on the bottom surface of the base shell (411), the grabbing member (412) is clamped on the outer surface of the freezing shell (211) of the freezing assembly at a side away from the base shell (411), a plurality of freezing tubes (212) are installed in the freezing shell (211) at intervals, and the sides of the tube grabbing members (413) away from the base shell (411) are inserted and fixed in the freezing tubes (212) one by one.

2. The cell culture automatic operation equipment according to claim 1, characterized in that: The thawing and opening mechanism (500) cooperates with the batch grabbing mechanism (410) to complete the thawing operation of the frozen storage component; the cell culture automatic operation equipment also includes a centrifuge (50) and a microscope (5000), and the preparation of the suspended cell fluid of the frozen storage component is completed by the mutual cooperation of the clamping mechanism (420), the pipetting mechanism (430) and the centrifuge (50), and the suspended cell fluid is filled with reagents for culture after being divided and the growth state of the cells is observed in real time by the microscope (5000) during the culture process.

3. The cell culture automatic operation equipment according to claim 1, characterized in that: The batch grabbing mechanism (410) further comprises a cylinder (414) arranged on a side of the base shell (411) away from the pipe grabbing member (413), a moving member fixedly connected to an output end of the cylinder (414) and installed in the base shell (411), and the moving member and the grabbing member (412) are arranged to slide relative to each other.

4. The cell culture automatic operation equipment according to claim 1, characterized in that: The clamping mechanism (420) includes a first driving member (421) and a plurality of clamping groups (422) fixed to the first driving member (421), each of the clamping groups (422) being clamped on the outer surface of the centrifuge tube or the culture storage mechanism, and each of the clamping groups (422) including a plurality of clamping members (423) arranged in a surrounding manner along the side of the first driving member (421).

5. The cell culture automatic operation equipment according to claim 1, characterized in that: The consumable storage module (600) includes a carrier (610) for carrying pipette tips, and at least one positioning frame (620) installed on the carrier (610), each of the positioning frames (620) includes a positioning seat fixed on the carrier (610), and a pressure piece rotatably arranged on the positioning seat, and the pressure piece is in contact with a consumable shell (20) for mounting a centrifuge tube (10).

6. The cell culture automatic operation equipment according to claim 1, characterized in that: The clamping and moving installation module (3000) includes a mounting frame (3001) installed in the housing module (100), and a mounting member (3002) arranged on the mounting frame (3001). The mounting member (3002) is engaged with a positioning member (3003) on the clamping and moving module (400), thereby realizing the storage of the clamping and moving installation module (3000).

7. The cell culture automatic operation equipment according to claim 1, characterized in that: The liquid processing module (1000) is used to transfer the reagent into the culture bottle (220) or the centrifuge tube (10), and comprises a pipette line (1001), a heating component (1100) and a pump body (1002) both installed on the pipette line (1001), and a sample adding component connected to one end of the pipette line (1001), and the other end of the pipette line (1001) is connected to the culture bottle (220) or the centrifuge tube (10).

8. The cell culture automatic operation equipment according to claim 1, characterized in that: The solid processing module (700) is used to recycle discarded culture carriers or centrifuge tubes (10) or pipette tips (30); the waste liquid processing module (2000) is used to process waste dumped from the centrifuge tubes (10) or the culture bottles (220); the conveying module (800) is used to transport culture samples in and out of the housing module (100); the opening and closing cover mechanism (900) is used for opening and closing the culture bottles (220) or centrifuge tubes (10); the environmental control module (4000) is used to maintain the environmental state required during the cell culture operation; the solid processing module (700), the waste liquid processing module (2000), the conveying module (800), and the opening and closing cover mechanism (900) are all installed in the housing module (100).

9. The cell culture automatic operation equipment according to claim 8, characterized in that: The cell culture automatic operation equipment also includes a cold storage refrigerator (40) for storing experimental reagents, and an environmental control module (4000) for maintaining the environmental state required during the cell culture operation process.

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