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An adiponectin and kit technology, which is applied in the measurement of color/spectral properties, etc., can solve the problems of kit stability, repeatability and accuracy can not meet the requirements, etc., to improve anti-interference ability, improve stability, make low cost effect
Active Publication Date: 2022-06-21
浙江夸克生物科技有限公司
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However, the stability, repeatability and accuracy of the kit cannot meet the needs
Method used
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Embodiment 1
[0063] Embodiment 1 A kind of adiponectin assay kit
[0064] The components and their concentrations are as follows:
[0066] R2 reagents include: anti-human adiponectin antibody latex particles 8 mg / L, pH=7 tris 90 mmol / L, Tween-80 60 g / L and Proclin-300 0.1 g / L.
Embodiment 2
[0067] Embodiment 2 A kind of adiponectin assay kit
[0068] The components and their concentrations are as follows:
[0070] R2 reagents include: anti-human adiponectin antibody latex particles 12 mg / L, pH=7 tris 120 mmol / L, Tween-80 100 g / L and Proclin-300 0.5 g / L.
Embodiment 3
[0071] Embodiment 3 A kind of adiponectin assay kit
[0072] The components and their concentrations are as follows:
[0074] R2 reagents include: anti-human adiponectin antibody latex particles 9mg / L, pH=7 Tris 110mmol / L, Tween-80 90g / L and Proclin-300 0.3g / L
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Abstract
The invention provides an adiponectin assay kit and application thereof, and relates to the technical field of biological detection. In the present invention, by adding a stabilizer and a surfactant to the reagent R1, the stabilizer is controlled to be mannitol and chitosan with a mass ratio of 1-5:1, and the surfactant is a sophorolipid with a mass ratio of 1:1-3:5 , sodium dodecylbenzenesulfonate and polyethylene glycol 6000, and control the specific components and concentration ratio of the two, so that the sensitivity and accuracy of the kit are significantly increased; by controlling the sophorolipid and chitosan The mass ratio can significantly improve the stability of the kit. Even in the case of reagent R1 without preservatives, the kit still has good stability at 2-8°C for 16 months in the dark and airtight storage.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to an adiponectin assay kit and application thereof. Background technique [0002] Adiponectin is an adipocytokine that can exist stably in human plasma with a concentration range of about 3.0-30.0 mg / L, accounting for about 0.01% of plasma protein. Human adiponectin contains 244 amino acids and consists of 3 regions: an amino-terminal signal sequence, a collagen domain, and a carboxy-terminal globular domain. Adiponectin exists in blood as three isoforms: trimers (65kb), hexamers (150kb) and high molecular weight polymers (18-36 multimers, >280kb). Clinical studies have shown that adiponectin is closely related to obesity, type II diabetes, coronary heart disease, and insulin resistance, and has anti-atherosclerosis, anti-inflammatory and anti-intimal hyperplasia properties after vascular injury. [0003] There are various assay methods for adiponectin, such as ra...
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