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A seborrhea measurement kit and its application

An adiponectin and kit technology, which is applied in the measurement of color/spectral properties, etc., can solve the problems of kit stability, repeatability and accuracy can not meet the requirements, etc., to improve anti-interference ability, improve stability, make low cost effect

Active Publication Date: 2022-06-21
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stability, repeatability and accuracy of the kit cannot meet the needs

Method used

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  • A seborrhea measurement kit and its application
  • A seborrhea measurement kit and its application
  • A seborrhea measurement kit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 A kind of adiponectin assay kit

[0064] The components and their concentrations are as follows:

[0065] R1 reagent includes: pH=7 Tris 90mmol / L, Sodium Chloride 3g / L, Mannitol 2g / L, Chitosan 1g / L, Sophorolipid 10g / L, Dodecylbenzene Sodium sulfonate 20g / L and polyethylene glycol 6000 50g / L;

[0066] R2 reagents include: anti-human adiponectin antibody latex particles 8 mg / L, pH=7 tris 90 mmol / L, Tween-80 60 g / L and Proclin-300 0.1 g / L.

Embodiment 2

[0067] Embodiment 2 A kind of adiponectin assay kit

[0068] The components and their concentrations are as follows:

[0069] R1 reagent includes: pH=7 Tris 120mmol / L, Sodium Chloride 8g / L, Mannitol 5g / L, Chitosan 3g / L, Sophorolipid 15g / L, Dodecylbenzene Sodium sulfonate 40g / L and polyethylene glycol 6000 80g / L;

[0070] R2 reagents include: anti-human adiponectin antibody latex particles 12 mg / L, pH=7 tris 120 mmol / L, Tween-80 100 g / L and Proclin-300 0.5 g / L.

Embodiment 3

[0071] Embodiment 3 A kind of adiponectin assay kit

[0072] The components and their concentrations are as follows:

[0073] R1 reagent includes: pH=7 Tris 110mmol / L, Sodium Chloride 6g / L, Mannitol 4g / L, Chitosan 2g / L, Sophorolipid 13g / L, Dodecylbenzene Sodium sulfonate 30g / L and polyethylene glycol 600075g / L;

[0074] R2 reagents include: anti-human adiponectin antibody latex particles 9mg / L, pH=7 Tris 110mmol / L, Tween-80 90g / L and Proclin-300 0.3g / L

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Abstract

The invention provides an adiponectin assay kit and application thereof, and relates to the technical field of biological detection. In the present invention, by adding a stabilizer and a surfactant to the reagent R1, the stabilizer is controlled to be mannitol and chitosan with a mass ratio of 1-5:1, and the surfactant is a sophorolipid with a mass ratio of 1:1-3:5 , sodium dodecylbenzenesulfonate and polyethylene glycol 6000, and control the specific components and concentration ratio of the two, so that the sensitivity and accuracy of the kit are significantly increased; by controlling the sophorolipid and chitosan The mass ratio can significantly improve the stability of the kit. Even in the case of reagent R1 without preservatives, the kit still has good stability at 2-8°C for 16 months in the dark and airtight storage.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an adiponectin assay kit and application thereof. Background technique [0002] Adiponectin is an adipocytokine that can exist stably in human plasma with a concentration range of about 3.0-30.0 mg / L, accounting for about 0.01% of plasma protein. Human adiponectin contains 244 amino acids and consists of 3 regions: an amino-terminal signal sequence, a collagen domain, and a carboxy-terminal globular domain. Adiponectin exists in blood as three isoforms: trimers (65kb), hexamers (150kb) and high molecular weight polymers (18-36 multimers, >280kb). Clinical studies have shown that adiponectin is closely related to obesity, type II diabetes, coronary heart disease, and insulin resistance, and has anti-atherosclerosis, anti-inflammatory and anti-intimal hyperplasia properties after vascular injury. [0003] There are various assay methods for adiponectin, such as ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 林耀文王志
Owner 浙江夸克生物科技有限公司
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