Field automatic photosynthetic instrument
By designing an automatic field photosynthesis instrument, unattended long-term monitoring and simultaneous measurement of gas exchange and chlorophyll fluorescence are achieved, which solves the problem that unattended and medium-scale plant measurements in the existing technology is unable to achieve measurement efficiency and accuracy in the measurement , suitable for measurement of single leaves and entire branches.
Patent Information
- Application Number
- CN202411413626.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-11
- Publication Date
- 2024-12-06
AI Technical Summary
The prior art cannot realize unattended automatic long-term monitoring in the field, and lacks photosynthesis measurement methods for medium-scale plants such as individual branches, so it is impossible to measure gas exchange and chlorophyll fluorescence simultaneously.
An automatic field photosynthesiser is designed, including a base, chlorophyll fluorescence detection assembly, gas exchange detection assembly and mobile mechanism, which can automatically perform measurements without human intervention and supports individual or simultaneous gas exchange and chlorophyll fluorescence measurements, maintaining The natural light state of the plant reduces the impact on plant growth.
It realizes automatic monitoring without guarding in the field for a long time, adds measurement parameters, improves measurement efficiency and accuracy, is suitable for the measurement of individual leaves and entire branches, reduces the impact on plant growth, and saves manpower, material resources and financial resources.
Smart Images

Figure CN119086839A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of plant physiological measurement, and further to a field automatic photosynthesis instrument. Background Art
[0002] Photosynthesis is the most important life process on Earth and has always been a hot topic in scientific research. The main methods for measuring photosynthesis are gas exchange method and chlorophyll fluorescence method, and both methods can also be used for simultaneous measurement. However, in the prior art, simultaneous measurement of both usually requires manual intervention, which is time-consuming and labor-intensive, and cannot achieve unmanned automatic long-term monitoring in the field. In addition, the prior art has measurements on smaller scales such as single leaves, and also on larger scales such as whole plants or groups, but there is no measurement on medium scales such as single branches.
[0003] Therefore, there is an urgent need to develop an automatic photosynthetic instrument that can not only meet the requirements of long-term unattended monitoring in the field, but also simultaneously measure gas exchange and chlorophyll fluorescence. Summary of the invention
[0004] In view of the above technical problems, the purpose of the present invention is to provide a field automatic photosynthesis instrument that can not only meet the needs of long-term unattended monitoring in the field, but also measure gas exchange and chlorophyll fluorescence, and the two measurements can be performed separately or simultaneously, which is convenient for comparison and analysis of different measurement results.
[0005] In order to achieve the above object, the present invention provides a field automatic photosynthesis instrument, comprising:
[0006] A base, wherein the base is provided with a fixing clamp and a sample port, a sealing member is provided at the sample port, the sealing member is used to seal the sample port, and the fixing clamp is used to fix the plant to be tested;
[0007] A chlorophyll fluorescence detection component is disposed on the base, and the chlorophyll fluorescence detection component is used to measure photosynthesis of the plant to be tested;
[0008] A gas exchange detection component is disposed on the base, and the gas exchange detection component is used to measure photosynthesis of the plant to be tested;
[0009] A box body, wherein the box body is a transparent structure and an opening is provided on one side of the box body;
[0010] A moving mechanism, the moving mechanism is connected to the base and the box respectively, and the moving mechanism can drive the box to move towards or away from the base;
[0011] When the moving mechanism drives the box to move a preset distance in a direction close to the base, the base is adapted to be connected with the opening, so that the box forms a closed structure, and the fixing clamp, the detection part of the chlorophyll fluorescence detection component, and the collection part of the gas exchange detection component are all located in the box;
[0012] When the moving mechanism drives the box to move a preset distance in a direction away from the base, the base is separated from the opening, so that the box forms an open structure.
[0013] In some embodiments, the sealing member comprises a soft film, a slit is provided in the middle of the soft film, the plant to be tested can pass through the slit and extend into the box, and the slit can remain sealed.
[0014] In some embodiments, the fixing clamp includes a first clamping portion and a second clamping portion, the first clamping portion and the second clamping portion are hollow structures that can transmit light, and the first clamping portion and the second clamping portion can be opened and closed to clamp and fix the plant to be tested.
[0015] In some embodiments, the fixing clamp also includes a fixing rod and a hinge, one end of the fixing rod is fixed to the base, the other end of the fixing rod is fixedly connected to the first clamping part, and the hinge is respectively connected to the first clamping part and the second clamping part, so that the second clamping part can be opened or closed relative to the first clamping part.
[0016] In some embodiments, the chlorophyll fluorescence detection component includes a chlorophyll fluorescence probe and an optical fiber, the chlorophyll fluorescence probe is disposed on the base, the optical fiber is connected to the chlorophyll fluorescence probe, and the optical fiber and the fixing clamp are disposed at a preset angle, so that the optical fiber can be close to the light-facing surface of the leaves of the plant to be tested to detect its chlorophyll fluorescence signal under natural light.
[0017] In some embodiments, the gas exchange detection assembly includes a gas sampling tube, which passes through the base, one end of which is used to extend into the box, and the other end of which is used to connect to the photosynthesis instrument host.
[0018] In some embodiments, it further comprises:
[0019] A fan is disposed in the box and is used to mix the gas concentration in the box.
[0020] In some embodiments, the box body is a square structure, a fixing portion is disposed at the bottom of the box body, the moving mechanism is mounted on the base, and a moving end of the moving mechanism is connected to the fixing portion.
[0021] In some embodiments, the moving mechanism is an electric guide rail, which includes an electric guide rail body and a moving end. The electric guide rail body is fixed on the base, and the moving end is fixed on the fixed part. The electric guide rail body can drive the moving end to reciprocate along the axial direction of the electric guide rail body.
[0022] In some embodiments, it further comprises:
[0023] A control component is connected to the electric guide rail and is used to control the operation of the electric guide rail.
[0024] Compared with the prior art, the field automatic photosynthesis instrument provided by the present invention has at least one of the following beneficial effects:
[0025] 1. The field automatic photosynthesis instrument provided by the present invention can not only meet the requirements of long-term unattended monitoring in the field, but also measure gas exchange and chlorophyll fluorescence. The two measurements can be performed separately or simultaneously, which is convenient for comparison and analysis between different measurement results. Chlorophyll fluorescence can be measured while measuring photosynthesis, which increases the number of measurement parameters and improves measurement efficiency.
[0026] 2. The field automatic photosynthetic instrument provided by the present invention can measure a single leaf of a plant or the entire branch, and has a wider scope of application; the measurement can be carried out in the sun to maintain the natural lighting state of the plant, thereby improving the accuracy and authenticity of the measurement results; in addition, during the measurement gap, the box can be in an open state, so that the plant branches and leaves are in a natural environment, reducing the impact of the measurement on the growth of the plant, and further improving the accuracy and authenticity of the measurement results;
[0027] 3. The field automatic photosynthesis instrument provided by the present invention has a box that can be automatically opened and closed by an electric guide rail according to the set interval, thereby realizing unattended automatic monitoring and can perform automatic measurements in the field for a long time without human intervention, thus saving manpower, material and financial resources. The field automatic photosynthesis instrument has low cost, good effect and high comprehensive benefits. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] The preferred implementation modes will be described below in a clear and understandable manner with reference to the accompanying drawings to further illustrate the above-mentioned characteristics, technical features, advantages and implementation methods of the present invention.
[0029] Figure 1 It is a structural schematic diagram of a field automatic photosynthetic instrument according to a preferred embodiment of the present invention.
[0030] Description of Figure Numbers:
[0031] Base 1, fixing clamp 11, sealing member 12, slit 121, chlorophyll fluorescence detection assembly 2, chlorophyll fluorescence probe 21, optical fiber 22, gas exchange detection assembly 3, gas sampling tube 31, housing 4, fan 41, fixing portion 42, moving mechanism 5, electric guide rail body 51, moving end 52, and plant to be tested 6. DETAILED DESCRIPTION
[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the specific implementation methods of the present invention will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings and other implementation methods can be obtained based on these drawings without creative work.
[0033] In order to simplify the drawings, only the parts related to the invention are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically drawn or marked. In this article, "one" not only means "only one", but also means "more than one".
[0034] It should be further understood that the term “and / or” used in the specification and appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.
[0035] In this document, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0036] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.
[0037] In one embodiment, the reference specification Figure 1The present invention provides an automatic outdoor photosynthesis instrument, comprising: a base 1, a chlorophyll fluorescence detection component 2, a gas exchange detection component 3, a box 4 and a moving mechanism 5, wherein the base 1 is provided with a fixing clamp 11 and a sample port, a sealing member 12 is provided at the sample port, the sealing member 12 is used to seal the sample port, and the fixing clamp 11 is used to fix the plant 6 to be tested; the chlorophyll fluorescence detection component 2 is provided on the base 1, and the chlorophyll fluorescence detection component 2 is used to measure the photosynthesis of the plant 6 to be tested; the gas exchange detection component 3 is provided on the base 1, and the gas exchange detection component 3 is used to measure the photosynthesis of the plant 6 to be tested; the box 4 is a transparent structure, and an opening is provided on one side of the box 4; the moving mechanism 5 is respectively connected to the base 1 and the box 4, and the moving mechanism 5 can drive the box 4 to move in a direction close to or away from the base 1;
[0038] When the moving mechanism 5 drives the box body 4 to move a preset distance in the direction close to the base 1, the base 1 is adapted to be connected with the opening, so that the box body 4 forms a closed structure, and the fixing clamp 11, the detection part of the chlorophyll fluorescence detection component 2, and the collection part of the gas exchange detection component 3 are all located in the box body 4; when the moving mechanism 5 drives the box body 4 to move a preset distance in the direction away from the base 1, the base 1 is separated from the opening, so that the box body 4 forms an open structure.
[0039] In this embodiment, the field automatic photosynthesis instrument can not only meet the needs of long-term unattended monitoring in the field, but also measure gas exchange and chlorophyll fluorescence, and the two measurements can be performed separately or simultaneously, which is convenient for comparison and analysis between different measurement results. Chlorophyll fluorescence can also be measured while measuring photosynthesis, which increases the number of measurement parameters and improves measurement efficiency.
[0040] Specifically, refer to the attached manual Figure 1 The seal 12 includes a soft film, and a slit 121 is provided in the middle of the soft film. The plant 6 to be tested can pass through the slit 121 and extend into the box body 4, and the slit 121 can be kept sealed. The seal 12 allows the plant 6 to pass through while avoiding air leakage. The soft film is a deformable soft material, such as a rubber film. Of course, the seal 12 can also be set to an openable and closable structure, as long as it can achieve a structure or device that can keep the sample port sealed after the plant 6 to be tested passes through.
[0041] Reference Manual Attached Figure 1 The fixing clamp 11 includes a first clamping part and a second clamping part. The first clamping part and the second clamping part are hollow structures that can transmit light. The first clamping part and the second clamping part can be opened and closed, so as to clamp and fix the plant to be tested 6. For example, the first clamping part and the second clamping part are square frame structures, and a transparent net is arranged inside the square frame structure. The transparent net can be made of materials such as transparent fishing line. The first clamping part and the second clamping part can also be set as other hollow structures that can transmit light.
[0042] The fixing clamp 11 further includes a fixing rod and a hinge, one end of the fixing rod is fixed on the base 1, the other end of the fixing rod is fixedly connected to the first clamping part, and the hinge is respectively connected to the first clamping part and the second clamping part, so that the second clamping part can be opened or closed relative to the first clamping part. The hinge can be arranged at one end of the first clamping part away from the fixing rod, and the hinge can also be arranged on both sides of the first clamping part. The fixing clamp 11 is preferably placed horizontally, which is conducive to the branches and leaves of the plant 6 to be tested to receive sunlight and maintain photosynthesis.
[0043] The hollow part of the fixing clip 11 is required to be strong, not easy to be broken, transparent, and not to block light. Nylon material can also be used. The fixing clip 11 can make the leaves of the plant 6 to be measured flat when fixed, which is convenient for measurement. It can also be applicable to curled or uneven leaves, making it more suitable for photosynthesis measurement. The plant 6 to be measured can be a single leaf, a whole branch, or even some medium-sized whole plants.
[0044] It should be pointed out that the specific structure of the fixing clamp 11 is described in accordance with the drawings in the specification. In actual use, other structures may also be used, as long as the structure or device can be used to clamp and fix the plant 6 to be tested and does not affect the photosynthesis of the plant 6 to be tested. This is only for the purpose of better illustrating the present invention and should not constitute a limitation to the present invention.
[0045] Reference Manual Attached Figure 1 The chlorophyll fluorescence detection component 2 includes a chlorophyll fluorescence probe 21 and an optical fiber 22. The chlorophyll fluorescence probe 21 is arranged on the base 1. The optical fiber 22 is connected to the chlorophyll fluorescence probe 21, and the optical fiber 22 and the fixing clamp 11 are arranged at a preset angle, so that the optical fiber 22 can be close to the light-facing surface of the leaves of the plant to be tested 6 to detect its chlorophyll fluorescence signal under natural light. The angle between the optical fiber 22 and the fixing clamp 11 is 30°-60°, such as 30°, 40°, 45°, 50°, 55°, etc. Of course, the chlorophyll fluorescence detection component 2 can also use other existing devices, as long as the device can detect the plant to be tested 6 on the fixing clamp 11.
[0046] Reference Manual Attached Figure 1 The gas exchange detection assembly 3 includes a gas sampling tube 31, which penetrates the base 1, one end of which is used to extend into the box 4, and the other end of which is used to connect to the photosynthetic instrument host. The photosynthetic instrument host extracts gas in the box 4 through the gas collection tube 31, analyzes the change of the carbon dioxide concentration of the gas, and calculates the photosynthetic rate of the plant 6 to be tested.
[0047] Reference Manual Attached Figure 1The field automatic photosynthesis instrument further includes: a fan 41, which is arranged in the box 4 and is used to mix the gas concentration in the box 4. By arranging the fan 41 in the box 4, the air in the box 4 can be made more uniform, further restore the natural conditions of measurement, and improve the accuracy of photosynthesis measurement.
[0048] In one embodiment, the reference specification Figure 1 On the basis of the above embodiment, the box body 4 is a square structure. Of course, the box body 4 can also be set to structures of other shapes, such as a hexagonal structure, a trapezoidal structure, etc. A fixing portion 42 is provided at the bottom of the box body 4, and the moving mechanism 5 is installed on the base 1, and the moving end of the moving mechanism 5 is connected to the fixing portion 42. The fixing portion 42 includes two fixing plates arranged in parallel and spaced apart, and the two fixing plates are fixed to the bottom of the box body 4. Of course, the fixing portion 42 can also be set to other structures, as long as the structure can achieve the fixing of the moving end of the moving mechanism 5.
[0049] Specifically, the moving mechanism 5 is an electric guide rail, which includes an electric guide rail body 51 and a moving end 52. The electric guide rail body 51 is fixed on the base 1, and the moving end 52 is fixed on the fixed portion 42. The electric guide rail body 51 can drive the moving end 52 to reciprocate along the axis direction of the electric guide rail body 51. The moving mechanism 5 can also adopt an electric push rod structure, an electric lead screw nut structure, etc., as long as the structure or device can realize the driving box body 4 to move in the direction close to or away from the base 1.
[0050] Furthermore, the field automatic photosynthetic instrument also includes: a control component, which is connected to the electric guide rail and is used to control the operation of the electric guide rail so that the electric guide rail can automatically open or close the box 4 according to the set interval time, thereby realizing unattended automatic monitoring.
[0051] In this embodiment, the field automatic photosynthetic instrument can measure a single leaf of a plant or an entire branch, and has a wider range of applications. The measurement can be carried out in the sun to maintain the natural lighting state of the plant, thereby improving the accuracy and authenticity of the measurement results. In addition, during the measurement gap, the box 4 can be in an open state to place the plant branches and leaves in a natural environment, thereby reducing the impact of the measurement on plant growth and further improving the accuracy and authenticity of the measurement results.
[0052] The box body 4 can also be automatically opened and closed by the electric guide rail according to the set interval, realizing unattended automatic monitoring, and can be automatically measured in the field for a long time without human intervention, saving manpower, material and financial resources. The field automatic photosynthesis instrument has low cost, good effect and high comprehensive benefits.
[0053] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.
[0054] It should be noted that the above embodiments can be freely combined as needed. The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. A field automatic photosynthetic instrument, characterized in that: include: A base, wherein the base is provided with a fixing clamp and a sample port, a sealing member is provided at the sample port, the sealing member is used to seal the sample port, and the fixing clamp is used to fix the plant to be tested; A chlorophyll fluorescence detection component is disposed on the base, and the chlorophyll fluorescence detection component is used to measure photosynthesis of the plant to be tested; A gas exchange detection component is disposed on the base, and the gas exchange detection component is used to measure photosynthesis of the plant to be tested; A box body, wherein the box body is a transparent structure and an opening is provided on one side of the box body; A moving mechanism, the moving mechanism is connected to the base and the box respectively, and the moving mechanism can drive the box to move towards or away from the base; When the moving mechanism drives the box to move a preset distance in a direction close to the base, the base is adapted to be connected with the opening, so that the box forms a closed structure, and the fixing clamp, the detection part of the chlorophyll fluorescence detection component, and the collection part of the gas exchange detection component are all located in the box; When the moving mechanism drives the box to move a preset distance in a direction away from the base, the base is separated from the opening, so that the box forms an open structure.
2. The field automatic photosynthesis instrument according to claim 1, characterized in that: The sealing member comprises a soft film, a slit is arranged in the middle of the soft film, the plant to be tested can pass through the slit and extend into the box body, and the slit can maintain sealing.
3. The field automatic photosynthesis instrument according to claim 1, characterized in that: The fixing clamp includes a first clamping portion and a second clamping portion. The first clamping portion and the second clamping portion are hollow structures that can transmit light. The first clamping portion and the second clamping portion can be opened and closed to clamp and fix the plant to be tested.
4. The field automatic photosynthesis instrument according to claim 3, characterized in that: The fixing clamp also includes a fixing rod and a hinge, one end of the fixing rod is fixed on the base, the other end of the fixing rod is fixedly connected to the first clamping part, and the hinge is respectively connected to the first clamping part and the second clamping part, so that the second clamping part can be opened or closed relative to the first clamping part.
5. The field automatic photosynthesis instrument according to claim 1, characterized in that: The chlorophyll fluorescence detection component includes a chlorophyll fluorescence probe and an optical fiber. The chlorophyll fluorescence probe is arranged on the base. The optical fiber is connected to the chlorophyll fluorescence probe, and the optical fiber and the fixing clamp are arranged at a preset angle so that the optical fiber can be close to the light-facing surface of the leaves of the plant to be tested to detect its chlorophyll fluorescence signal under natural light.
6. The field automatic photosynthesis instrument according to claim 1, characterized in that: The gas exchange detection component comprises a gas sampling tube, which passes through the base, one end of which is used to extend into the box, and the other end of which is used to be connected to the photosynthesis instrument host.
7. The field automatic photosynthesis instrument according to claim 1, characterized in that: Also includes: A fan is disposed in the box and is used to mix the gas concentration in the box.
8. The field automatic photosynthesis instrument according to any one of claims 1 to 7, characterized in that: The box body is a square structure, a fixing part is arranged at the bottom of the box body, the moving mechanism is installed on the base, and a moving end of the moving mechanism is connected to the fixing part.
9. The field automatic photosynthesis instrument according to claim 8, characterized in that: The moving mechanism is an electric guide rail, which includes an electric guide rail body and a moving end. The electric guide rail body is fixed on the base, and the moving end is fixed on the fixed part. The electric guide rail body can drive the moving end to reciprocate along the axial direction of the electric guide rail body.
10. The field automatic photosynthesis instrument according to claim 9, characterized in that: Also includes: A control component is connected to the electric guide rail and is used to control the operation of the electric guide rail.
