Composition with whitening and moisturizing effects

Abstract

The invention provides a composition with whitening and moisturizing effects, and relates to the technical field of cosmetics. The composition with the whitening and moisturizing effects comprises 50%-70% of a compound traditional Chinese medicine extract and 30%-50% of a hericium erinaceus mycelium extract, the compound traditional Chinese medicine extract comprises one or more of a liquorice extract, an angelica sinensis extract, a bletilla striata extract and an aloe extract. The product with good whitening and moisturizing effects is obtained by utilizing the synergistic effect of different raw materials, and the product is healthy and safe in raw material source, low in allergy risk and safer to use.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a composition with whitening and moisturizing effects. Background Art

[0002] Cosmetics refer to chemical industrial products or fine chemical products that are spread on any part of the human body surface, such as skin, hair, nails, lips and teeth, by smearing, spraying or other similar methods, for the purpose of cleaning, maintenance, beautification, modification and change of appearance, or correction of body odor and maintenance of good condition.

[0003] The Chinese beauty cosmetics market, known as the "beauty economy", has achieved unprecedented success after more than 20 years of rapid development. China's cosmetics market is the world's largest emerging market. In just over 20 years, China's cosmetics industry has grown from small to large, from weak to strong, from simple and extensive to leading technology and group management. The entire industry has formed a large and vibrant industrial army.

[0004] According to the usage, cosmetics are divided into skin cosmetics, hair cosmetics, beauty cosmetics and special functional cosmetics. Among them, skin cosmetics refer to cosmetics for face and skin. Such cosmetics include various creams, bath agents, etc.; hair cosmetics refer to special cosmetics for hair. Such cosmetics include shampoo, mousse, spray hair spray, etc.; beauty cosmetics refer to facial beauty products, including beauty products for nails and hair; special functional cosmetics refer to cosmetics with special drugs added. Skin cosmetics include skin care products, and their functions mainly include cleaning, moisturizing, oil control and acne removal, whitening and lightening, firming and anti-wrinkle, removing red blood streaks, repairing after sun exposure, anti-allergy, shrinking pores and repairing barriers. Among them, whitening and moisturizing are one of the skin care needs that consumers pay the most attention to, especially in the Asian market, the demand for whitening products is very high. Moisturizing is the basis for maintaining skin health. Lack of water can lead to problems such as dry skin and peeling, while whitening products can help even out skin tone and reduce spots.

[0005] As people pay more and more attention to skin care, the market demand for safe and effective whitening and moisturizing products is also increasing. Many traditional whitening ingredients and the use of various additives can have a negative impact on skin and overall health.

[0006] Therefore, developing green, healthy, safe and effective whitening and moisturizing products can meet consumer needs, reduce the occurrence of these risks and protect consumer health. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a composition with whitening and moisturizing effects, which utilizes the synergistic effect between different raw materials to obtain a product with good whitening and moisturizing effects. The raw materials of the product are healthy and safe, with low allergy risk, and safer to use.

[0008] The composition with whitening and moisturizing effects of the present invention comprises the following raw materials in percentage by weight:

[0009] 50% to 70% of compound Chinese herbal medicine extract and 30% to 50% of Hericium erinaceus mycelium extract.

[0010] Furthermore, the composite Chinese medicine extract includes one or more of licorice extract, angelica extract, bletilla striata extract, and aloe extract.

[0011] Furthermore, the composite Chinese medicine extract is composed of licorice extract, angelica extract, bletilla striata extract, and aloe extract in a mass ratio of (1-5):(1-3):(1-3):(1-5). Preferably, the composite Chinese medicine extract is composed of licorice extract, angelica extract, bletilla striata extract, and aloe extract in a mass ratio of 3:2:2:4.

[0012] The preparation methods of the licorice extract and the angelica extract are as follows:

[0013] Glycyrrhiza and Angelica sinensis are crushed respectively, and ethanol solution is used for reflux extraction. After the extraction is completed, the filter is filtered, and the filter residue is subjected to secondary extraction. The extracts are combined, concentrated, and dried to obtain licorice extract and angelica extract.

[0014] Furthermore, the concentration of the ethanol solution is 50% to 70%, the reflux extraction temperature is 30° C. to 65° C., and the extraction time is 1 to 3 hours. Preferably, the concentration of the ethanol solution is 60%, the reflux extraction temperature is 55° C., and the extraction time is 2 hours.

[0015] Furthermore, when the extraction raw material is liquorice, the ethanol solution contains 0.5%-2% ammonia water.

[0016] The preparation method of the bletilla striata extract is as follows: the bletilla striata tuber is crushed, then water extracted, filtered, the extract is concentrated, then ethanol is added for alcohol precipitation, and dried to obtain the bletilla striata extract.

[0017] Furthermore, the water extraction temperature is 60°C to 80°C, and the water extraction time is 1 to 4 hours. Preferably, the water extraction temperature is 70°C, and the water extraction time is 2.5 hours.

[0018] Further, the concentrated Bletilla striata extract is equivalent to 1-2g medicinal material / mL, and the ethanol concentration of the extract during the alcohol precipitation process is 60%-75%. Preferably, the concentrated Bletilla striata extract is equivalent to 2g medicinal material / mL, and the ethanol concentration of the extract during the alcohol precipitation process is 70%.

[0019] The preparation method of the aloe extract is as follows: peeling the aloe, cutting into pieces, adding water to boil, filtering, concentrating the filtrate, and drying to obtain the aloe extract.

[0020] Furthermore, the solid-liquid ratio of the aloe vera block to water is 1 g: (10-20) mL, and the boiling time is 15-45 min. Preferably, the solid-liquid ratio of the aloe vera block to water is 1 g: 15 mL, and the boiling time is 30 min.

[0021] The preparation method of the Hericium erinaceus mycelium extract is as follows:

[0022] S1, mycelium culture: activating and culturing the Hericium erinaceus strain, then inoculating it into a liquid culture medium for expansion culture to obtain a fermentation seed liquid, inoculating the fermentation seed liquid into a fermentation culture medium for fermentation, separating the culture medium after the fermentation is completed, and obtaining Hericium erinaceus mycelium;

[0023] S2, drying the mycelium of Hericium erinaceus and mixing it with an ethanol solution for extraction, filtering after the extraction is completed, repeating 2 to 3 times, combining the filtrate, removing the ethanol to obtain an extract;

[0024] S3, shaking and mixing the extract and petroleum ether for 10 minutes, and then standing for extraction, repeating 2 to 3 times, recovering the petroleum ether, concentrating and drying the extract, and obtaining the Hericium erinaceus extract.

[0025] Furthermore, the activation culture is carried out in an activation culture medium, which is composed of the following raw materials in percentage by mass: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, 2% agar, and 10% potato.

[0026] Furthermore, the activation temperature is 28°C to 35°C, and the activation time is 3 to 7 days. Preferably, the activation temperature is 32°C, and the activation time is 5 days.

[0027] Furthermore, the liquid culture medium is composed of the following raw materials in percentage by mass: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, and 10% potato.

[0028] Furthermore, the expansion culture temperature is 28°C to 35°C, and the expansion culture time is 2 to 4 days. Preferably, the expansion culture temperature is 32°C, and the expansion culture time is 3 days.

[0029] Furthermore, the fermentation medium is composed of the following raw materials in percentage by mass: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, 1% casein hydrolyzate, and 10% potato.

[0030] Furthermore, the fermentation temperature is 25°C to 35°C, the fermentation time is 5 to 10 days, and the inoculation amount of the fermentation seed liquid is 8% to 12%. Preferably, the fermentation temperature is 30°C, the fermentation time is 7 days, and the inoculation amount of the fermentation seed liquid is 10%.

[0031] Furthermore, the preparation method of the casein hydrolysate is as follows: casein is enzymatically hydrolyzed with trypsin at a pH of 7.4 to obtain the casein hydrolysate.

[0032] Further, the enzymatic hydrolysis temperature is 40°C to 45°C, the enzymatic hydrolysis time is 1 to 2 hours, the substrate concentration is 35 to 65 g / L, the enzyme activity of trypsin is 1.0 to 1.2 IU / mg, and the amount of trypsin used is 0.5 to 1.5‰ of casein. Preferably, the enzymatic hydrolysis temperature is 42°C, the enzymatic hydrolysis time is 1.5 hours, the substrate concentration is 50 g / L, the enzyme activity of trypsin is 1.1 IU / mg, and the amount of enzyme used is 1.0‰ of casein.

[0033] Further, the concentration of the ethanol solution is 70% to 80%, the extraction temperature is 30°C to 40°C, the extraction time is 1 to 3 hours, and the weight-volume ratio of Hericium erinaceus dry powder to the ethanol solution is 1g:(10-20)mL. Preferably, the concentration of the ethanol solution is 75%, the extraction temperature is 35°C, the extraction time is 2 hours, and the weight-volume ratio of Hericium erinaceus dry powder to the ethanol solution is 1g:15mL.

[0034] The composition with whitening and moisturizing effects of the present invention is used for preparing cosmetics with whitening and moisturizing effects.

[0035] Compared with the prior art, the beneficial technical effects of the present invention are:

[0036] The present invention utilizes the synergistic effect of the compound Chinese medicine extract and the Hericium erinaceus mycelium extract to effectively inhibit the generation of melanin and achieve a significant whitening effect.

[0037] The composition of the present invention not only has whitening and moisturizing effects, but also has multiple functions such as anti-inflammatory and antibacterial effects.

[0038] The raw materials used in the present invention are all natural plant extracts, which are highly safe and suitable for long-term use. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The present invention will be further described below in conjunction with the accompanying drawings.

[0040] Figure 1 The effect of the dosage of Hericium erinaceus mycelium extract on the whitening effect of the composition;

[0041] Figure 2 The invention discloses the influence of the dosage of Hericium erinaceus mycelium extract on the moisturizing effect of the composition. DETAILED DESCRIPTION

[0042] The technical solution provided by the present invention is further described below in conjunction with embodiments.

[0043] Unless otherwise specified, all experiments were repeated three times. SPSS21.0 was used for analysis of variance (ANOVA) and Duncan multiple comparison analysis, and the results were expressed as mean ± standard deviation. P < 0.05 indicated significant differences, and origin 8.0 was used for drawing.

[0044] Example 1 Preparation of composite Chinese herbal medicine extract

[0045] S1. Preparation of licorice extract: Grind licorice, use 60% ethanol solution containing 1.0% ammonia water to reflux extract at 55° C. for 2 hours, filter, perform secondary extraction on the residue, combine the extracts, concentrate, and dry to obtain licorice extract and angelica extract;

[0046] S2. Preparation of angelica extract: crush the angelica, use 60% ethanol solution to reflux extract at 55° C. for 2 hours, filter, perform secondary extraction on the residue, combine the extracts, concentrate, and dry to obtain licorice extract and angelica extract;

[0047] S3. Preparation of Bletilla striata extract: crush the tubers of Bletilla striata and extract them with water at 70°C for 2.5h, filter, concentrate the extract until the extract is equivalent to 2g medicinal material / mL, precipitate with 70% ethanol solution, and dry.

[0048] obtaining a bletilla striata extract;

[0049] S4. Preparation of aloe extract: peel the aloe vera, cut into blocks of 2 cm×2 cm×2 cm, add water at a ratio of 1 g:15 mL of aloe vera block to water, boil for 30 min, filter, concentrate the filtrate, and dry to obtain the aloe vera extract;

[0050] S5. Mix the liquorice extract, angelica extract, bletilla striata extract and aloe extract in a mass ratio of 3:2:2:4 to obtain a composite Chinese medicine extract.

[0051] Example 2 Preparation of Hericium erinaceus mycelium extract

[0052] S1, mycelium culture: the Hericium erinaceus strain was activated and cultured at 32°C for 5 days, and then inoculated into a liquid culture medium at 32°C for expansion culture for 3 days to obtain a fermentation seed liquid, and the fermentation seed liquid was inoculated into a fermentation medium at a seed amount of 10%, and fermented at 30°C for 7 days. After the fermentation was completed, the culture medium was separated to obtain Hericium erinaceus mycelium;

[0053] The activation medium is composed of the following raw materials in mass percentage: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, 2% agar, and 10% potato; the liquid medium is composed of the following raw materials in mass percentage: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, and 10% potato; the fermentation medium is composed of the following raw materials in mass percentage: 0.01% KH2PO4, 0.02% CaCl2, 0.1% MgSO4, 5% glucose, 1% casein hydrolysate, and 10% potato; the preparation method of the casein hydrolysate is as follows: trypsin with an enzyme activity of 1.1 IU / mg is inoculated into casein at an inoculation amount of 1.0‰, and enzymolysis is performed for 1.5 hours at a pH of 7.4 and 42°C to obtain casein hydrolysate;

[0054] S2, drying the mycelium of Hericium erinaceus and mixing it with a 75% ethanol solution at a weight volume ratio of 1 g: 15 mL, extracting at 35° C. for 2 h, filtering after the extraction, repeating 3 times, combining the filtrate, removing the ethanol to obtain an extract;

[0055] S3, shaking and mixing the extract and petroleum ether for 10 minutes, and then standing for extraction, repeating for 3 times, recovering the petroleum ether, concentrating and drying the extract, and obtaining the Hericium erinaceus extract.

[0056] Example 3 Effect of Hericium erinaceus mycelium extract on the effect of the composition

[0057] A composition with whitening and moisturizing effects was prepared using the composite Chinese medicine extract and Hericium erinaceus mycelium extract prepared in Example 1 and Example 2 as raw materials, and the mass percentage of Hericium erinaceus mycelium extract in the composition was controlled to be 10%, 20%, 30%, 40%, 50%, 60%, and 70%, respectively, and the whitening and moisturizing effects of the composition were measured.

[0058] (1) Tyrosinase inhibition rate

[0059] Prepare 476μg / mL tyrosinase solution, then dilute to 95.2μg / mL tyrosinase working solution, prepare 1mmol / L levodopa solution. Take 80μL of each composition sample (the composition is dissolved in 20 times deionized water to obtain the composition sample) into a 96-well plate, then add 120μL of 1mmo / L levodopa solution, and finally add 50μL of 95.2μg / mL tyrosinase solution, with deionized water as a control. React at 37℃ in the dark, and use an enzyme marker to measure the absorbance value of the sample at 475nm when the reaction is carried out for 0.5h, 1h, 2h, 4h, 8h, and 12h.

[0060] Tyrosinase inhibition rate (%) = 1-(sample absorbance value / control group absorbance value) × 100%

[0061] Test results such as Figure 1 shown.

[0062] Depend on Figure 1 It can be seen that the Hericium erinaceus mycelium extract has a significant effect on the tyrosinase inhibition rate of the composition, that is, the whitening effect. Too high or too low a content of Hericium erinaceus mycelium extract will affect the tyrosinase inhibition rate of the composition. This may be related to the ratio between the Hericium erinaceus mycelium extract and the composite Chinese medicine extract. Therefore, the present invention strictly limits the proportion of Hericium erinaceus mycelium extract in the composition with whitening and moisturizing effects of the present invention to 30% to 50%.

[0063] (2) Moisturizing effect

[0064] 80 healthy female subjects aged 35 to 40 years old with similar weight and skin quality were recruited on a voluntary basis. The subjects had healthy skin and no history of skin allergies, meeting the voluntary inclusion criteria. The subjects agreed to this trial and signed the informed consent form. The subjects had no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, and no history of severe allergies to skin care cosmetics. During the trial, the subjects were not allowed to apply any drugs or cosmetics not related to the trial.

[0065] The subjects were randomly divided into 8 groups, 10 people in each group, and used the hydrating gel prepared by each composition, once in the morning and once in the evening, with the same dosage, for 2 consecutive weeks. The content of the composition with whitening and moisturizing effects in each gel sample was 15%, and the hydrating gel without the composition was used as a control. After use, the skin moisture content was measured, and the change rate of skin moisture content before and after use was calculated.

[0066] Moisture content change rate (%) = (A2-A1) / A0

[0067] Among them: A2 is the skin moisture content of the experimental group; A1 is the skin moisture content of the control group; A0 is the skin moisture content before the experiment.

[0068] Test results such as Figure 2 shown.

[0069] Depend on Figure 2 It can be seen that the Hericium erinaceus mycelium extract has a significant effect on the moisturizing effect of the composition. Too high or too low a content of Hericium erinaceus mycelium extract will affect the moisturizing effect of the composition. However, unlike the whitening effect, when the amount of Hericium erinaceus mycelium extract is in the range of 30% to 60%, the composition with whitening and moisturizing effects has a good moisturizing effect. Moreover, during the use, the subjects did not have an allergic reaction, indicating that the composition of the present invention has a lower risk of allergies and is safer to use.

[0070] In summary, in order to ensure that the composition with whitening and moisturizing effects of the present invention has a good comprehensive effect, the present invention limits the amount of Hericium erinaceus mycelium extract to 30% to 50%.

[0071] Embodiment 4 A composition with whitening and moisturizing effects is composed of the following raw materials in percentage by mass:

[0072] Compound Chinese herbal medicine extract 70%, Hericium erinaceus mycelium extract 30%.

[0073] The composite Chinese medicine extract is prepared by the method described in Example 1, and the Hericium erinaceus mycelium extract is prepared by the method described in Example 2. Figure 1 , Figure 2 It can be seen that the tyrosinase inhibition rate of the composition is (82.5±1.3)%, and the moisturizing effect (expressed as the water content change rate) is (30.5±0.3)%.

[0074] Comparative Example 1

[0075] The same as Example 1, except that the fermentation medium does not contain casein hydrolyzate. After testing, the tyrosinase inhibition rate of the composition is (74.3±0.7)%, and the moisturizing effect (expressed by the water content change rate) is (28.1±0.5)%.

[0076] Comparative Example 2

[0077] The same as Example 1, except that: petroleum ether is not used to extract the extract in the preparation process of the Hericium erinaceus mycelium extract. After testing, the tyrosinase inhibition rate of the composition is (72.6±1.1)%, and the moisturizing effect (expressed by the water content change rate) is (28.9±0.6)%.

[0078] Comparative Example 3

[0079] The same as Example 1, except that no Hericium erinaceus mycelium extract was added. After testing, the tyrosinase inhibition rate of the composition was (53.6±1.6)%, and the moisturizing effect (expressed by the water content change rate) was (23.8±1.1)%.

[0080] Example 5

[0081] The same as Example 4, except that: the composite Chinese medicine extract is prepared by mixing licorice extract, angelica extract, bletilla striata extract, and aloe extract in a mass ratio of 1:1:1:1, and the preparation method of the licorice extract, angelica extract, bletilla striata extract, and aloe extract is the same as Example 1.

[0082] Example 6

[0083] Same as Example 4, except that: the composite Chinese medicine extract is prepared by mixing licorice extract, angelica extract, bletilla striata extract, and aloe extract in a mass ratio of 5:3:3:5, and the preparation method of the licorice extract, angelica extract, bletilla striata extract, and aloe extract is the same as Example 1.

[0084] Example 7

[0085] Same as Example 4, except that: during the preparation of the Hericium erinaceus mycelium extract, the extraction temperature was 30° C. and the extraction time was 3 h.

[0086] Example 8

[0087] Same as Example 4, except that: during the preparation of the Hericium erinaceus mycelium extract, the extraction temperature is 40° C. and the extraction time is 1 hour.

[0088] Example 9

[0089] The same as Example 4, except that: in the preparation process of the Hericium erinaceus mycelium extract, the weight volume ratio of Hericium erinaceus dry powder and ethanol solution is 1g:10mL.

[0090] Example 10

[0091] The same as Example 4, except that: in the preparation process of the Hericium erinaceus mycelium extract, the weight volume ratio of Hericium erinaceus dry powder and ethanol solution is 1g:20mL.

[0092] After testing, the whitening and moisturizing effects of the compositions with whitening and moisturizing effects prepared in Examples 5-10 were not significantly different from those of the product in Example 4.

[0093] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A composition with whitening and moisturizing effects, It is characterized in that The following raw materials are included in mass percentage: 50% to 70% of compound Chinese herbal medicine extract, 30% to 50% of Hericium erinaceus mycelium extract; The composite Chinese medicine extract comprises one or more of liquorice extract, angelica extract, bletilla striata extract and aloe extract.

2. The composition according to claim 1, It is characterized in that The composite Chinese medicine extract is composed of liquorice extract, angelica extract, bletilla striata extract and aloe extract in a mass ratio of (1-5):(1-3):(1-3):(1-5).

3. The composition according to claim 1, It is characterized in that The preparation method of the Hericium erinaceus mycelium extract is as follows: S1, mycelium culture: activating and culturing the Hericium erinaceus strain, then inoculating it into a liquid culture medium for expansion culture to obtain a fermentation seed liquid, inoculating the fermentation seed liquid into a fermentation culture medium for fermentation, separating the culture medium after the fermentation is completed, and obtaining Hericium erinaceus mycelium; S2, drying the mycelium of Hericium erinaceus and mixing it with an ethanol solution for extraction, filtering after the extraction is completed, repeating 2 to 3 times, combining the filtrate, removing the ethanol to obtain an extract; S3, mixing the extract and petroleum ether by oscillation for 10 minutes and then standing for extraction, repeating 2 to 3 times, recovering the petroleum ether, concentrating and drying the extract, and obtaining the Hericium erinaceus extract.

4. The composition according to claim 3, It is characterized in that The activation temperature is 28° C. to 35° C., and the activation time is 3 to 7 days.

5. The composition according to claim 3, It is characterized in that The expansion culture temperature is 28° C. to 35° C., and the expansion culture time is 2 to 4 days.

6. The composition according to claim 3, It is characterized in that The fermentation medium is composed of the following raw materials in percentage by mass: 0.01% KH 2 PO 4 , 0.02% CaCl 2 , 0.1% MgSO 4 , 5% glucose, 1% casein hydrolysate, 10% potato.

7. The composition according to claim 3, It is characterized in that The fermentation temperature is 25° C. to 35° C., the fermentation time is 5 to 10 days, and the inoculation amount of the fermentation seed liquid is 8% to 12%.

8. The composition according to claim 6, It is characterized in that The preparation method of the casein hydrolysate is as follows: casein is enzymatically hydrolyzed with trypsin at a pH of 7.4 to obtain the casein hydrolysate.

9. The composition according to claim 8, It is characterized in that The enzymatic hydrolysis temperature is 40°C to 45°C, the enzymatic hydrolysis time is 1 to 2 hours, the substrate concentration is 35 to 65 g / L, the enzyme activity of trypsin is 1.0 to 1.2 IU / mg, and the amount of trypsin used is 0.5 to 1.5‰ of casein.

10. The composition according to claim 3, It is characterized in that The concentration of the ethanol solution is 70% to 80%, the extraction temperature is 30° C. to 40° C., the extraction time is 1 to 3 hours, and the weight-to-volume ratio of Hericium erinaceus dry powder to the ethanol solution is 1 g: (10 to 20) mL.

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