Antigen emulsifying device

Abstract

The present invention relates to an antigen emulsification apparatus, which comprises: a housing in which a cavity is formed; the power assembly is arranged in the cavity and located on the upper portion of the cavity. The test tube is arranged in the cavity and located at the lower part of the cavity, and a sample containing an antigen and an immunologic adjuvant is preset in the test tube; the injector comprises a barrel, an injection part, a piston and a push rod, the injection part is connected to the barrel and inserted into the test tube, the piston is arranged in the barrel and connected to the push rod, and the push rod is connected to the power assembly and is configured to drive the piston to reciprocate under the driving of the power assembly; according to the device, the power assembly is used for automatically injecting and pushing the antigen and the immunologic adjuvant in the test tube, so that the automation degree is high, manpower is saved, the emulsification efficiency is greatly improved, the injection and pushing operation is more standard, the uniformity is good, and the efficiency is high. The antigen emulsification effect is improved, and the retention time of the antigen in the body is prolonged.

Description

Technical Field

[0001] The invention relates to the technical field of immunology, and in particular to an antigen emulsification device. Background Art

[0002] The development of immunology has greatly promoted the development of modern biology and medicine, and promoted the development of biological high-tech industries. The first step in antibody preparation - antigen immunization is a key step in immunological detection technology. Before antigen immunization, the antigen generally needs to be emulsified with an immune adjuvant, such as Freund's adjuvant, to make an "oil-in-water" emulsion, and then injected for immunization. A good emulsification effect can delay the release of the antigen, prolong the retention time of the antigen in the body, avoid frequent injections, and is conducive to the production of high-affinity antibodies. In some related technologies, antigen emulsification is performed manually, which is time-consuming and labor-intensive, inefficient, and may result in poor uniformity of antigen emulsification effects in different batches due to non-standard operations. Summary of the invention

[0003] Some embodiments of the present invention provide an antigen emulsification device for alleviating the problem of low efficiency of antigen emulsification operation.

[0004] In one aspect of the present invention, there is provided an antigen emulsification device, comprising:

[0005] a housing having a cavity formed therein;

[0006] A power assembly is disposed in the cavity and located at an upper portion of the cavity;

[0007] a test tube, disposed in the cavity and located at the lower part of the cavity, wherein a sample including an antigen and an immune adjuvant is pre-placed in the test tube; and

[0008] The syringe comprises a barrel, an injection part, a piston and a push rod, wherein the injection part is connected to the barrel and inserted into the test tube, the piston is arranged in the barrel and connected to the push rod, the push rod is connected to the power assembly and is configured to drive the piston to reciprocate under the drive of the power assembly to suck the sample in the test tube into the barrel or push the sample in the barrel back into the test tube.

[0009] In some embodiments, the antigen emulsification device further comprises a temperature regulating component disposed in the cavity, wherein the temperature regulating component is disposed around the test tube to regulate the temperature of the sample in the test tube.

[0010] In some embodiments, the temperature control component includes a heat conductive member and a semiconductor refrigeration sheet. The heat conductive member is provided with a groove for accommodating the test tube. The groove is configured to be pre-filled with a refrigerant. The semiconductor refrigeration sheet is disposed at the bottom of the heat conductive member and is configured to lower the temperature of the refrigerant.

[0011] In some embodiments, the temperature control component further includes a heat sink, and the heat sink is disposed on a side of the semiconductor refrigeration plate away from the heat conductive element.

[0012] In some embodiments, the power assembly includes a power member and a telescopic member, the telescopic member connects the power member and the push rod, and the power member drives the telescopic member to extend and retract, so that the telescopic member drives the push rod to reciprocate.

[0013] In some embodiments, the power assembly further comprises:

[0014] A shell is hollow inside, the top of the shell is connected to the top of the cavity, the bottom of the shell is open, and a notch is provided at the lower part of the shell, a first limiting portion is provided inside the notch, and the first limiting portion limits the end edge of the cylinder; and

[0015] The connecting member is arranged in the shell and connected to the telescopic member. The connecting member is provided with a second limiting portion, and the second limiting portion limits the end edge of the push rod.

[0016] In some embodiments, the first limiting portion includes two circles of bosses arranged at intervals, the bosses extend from the inner wall of the outer shell to the inside of the outer shell, and the end edge of the cylinder is arranged between the two circles of bosses.

[0017] In some embodiments, the connecting member includes a semi-ring portion, a closed portion is provided at one end of the semi-ring portion, the closed portion is connected to the telescopic member, the second limiting portion is provided at the second end of the semi-ring portion and extends toward the interior of the semi-ring portion, and the end edge of the push rod is provided between the second limiting portion and the closed portion.

[0018] In some embodiments, the antigen emulsification device further comprises a control component, wherein the control component comprises:

[0019] a controller electrically connected to the power assembly; and

[0020] A display screen is electrically connected to the controller, the display screen is arranged on the housing, and buttons for adjusting the emulsification frequency and time are arranged on the display screen.

[0021] In some embodiments, the housing includes an openable and closable cover plate, and the cover plate is provided with an observation window.

[0022] Based on the above technical solution, the present invention has at least the following beneficial effects:

[0023] In some embodiments, the antigen emulsification device drives the push rod to reciprocate through a power component, so that the push rod drives the piston to reciprocate to draw the sample in the test tube into the barrel or push the sample in the barrel back into the test tube, so that the antigen and immune adjuvant in the test tube are fully emulsified. The power component automatically injects and pushes the antigen and immune adjuvant in the test tube, with a high degree of automation, saving manpower, and greatly improving the emulsification efficiency. The power component can provide uniform power, making the injection and pumping actions more standardized and uniform, thereby improving the antigen emulsification effect, delaying the release of the antigen, and prolonging the residence time of the antigen in the body. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0025] Figure 1 A schematic diagram of an antigen emulsification device provided according to some embodiments of the present invention with a cover plate and a housing of a part of a power assembly removed;

[0026] Figure 2 This is a schematic diagram of an antigen emulsification device provided according to some embodiments of the present invention after a cover plate is installed;

[0027] Figure 3 A schematic diagram of removing a cover plate of an antigen emulsification device according to some embodiments of the present invention;

[0028] Figure 4 A schematic diagram of the connection state between a power assembly and a syringe according to some embodiments of the present invention;

[0029] Figure 5 It is a cross-sectional schematic diagram of the connection state between the power assembly and the syringe provided according to some embodiments of the present invention.

[0030] The reference numerals in the accompanying drawings are described as follows:

[0031] 1-shell; 11-cavity; 12-cover plate;

[0032] 2-power assembly; 21-power member; 22-telescopic member; 23-housing; 231-notch; 24-first limiting portion; 241-boss; 25-connecting member; 251-semi-ring portion; 252-closing portion; 253-second limiting portion;

[0033] 3- Test tube;

[0034] 4-syringe; 41-barrel; 42-injection part; 43-piston; 44-push rod;

[0035] 5-temperature regulating component; 51-semiconductor cooling sheet; 52-heat conducting member; 521-groove; 53-heat dissipating member;

[0036] 6-control component; 61-display screen;

[0037] 7- Bracket.

[0038] It should be understood that the size of each part shown in the accompanying drawings is not drawn according to the actual proportional relationship. In addition, the same or similar reference numerals represent the same or similar components. DETAILED DESCRIPTION

[0039] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the present invention and its application or use. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. These embodiments are provided to make the present invention thorough and complete and to fully express the scope of the present invention to those skilled in the art. It should be noted that unless otherwise specifically stated, the relative arrangement of the parts and steps, the composition of the materials, the numerical expressions and the numerical values ​​set forth in these embodiments should be interpreted as being merely exemplary, rather than as limitations.

[0040] The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different parts. The words "include" or "comprise" and similar words mean that the elements before the word include the elements listed after the word, and do not exclude the possibility of including other elements. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0041] In the present invention, when a specific device is described as being located between a first device and a second device, there may or may not be an intermediate device between the specific device and the first device or the second device. When a specific device is described as being connected to other devices, the specific device may be directly connected to the other devices without an intermediate device, or may not be directly connected to the other devices but have an intermediate device.

[0042] All terms (including technical terms or scientific terms) used in the present invention have the same meanings as those understood by ordinary technicians in the field to which the present invention belongs, unless otherwise specifically defined. It should also be understood that terms defined in general dictionaries should be interpreted as having meanings consistent with their meanings in the context of the relevant technology, and should not be interpreted in an idealized or extremely formal sense, unless explicitly defined herein.

[0043] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered as part of the specification.

[0044] Figure 1 Schematic diagram of the structure of some embodiments of the antigen emulsification device according to the present invention.

[0045] refer to Figure 1 In some embodiments, the antigen emulsification device includes a shell 1 , and a cavity 11 is formed in the shell 1 .

[0046] The antigen emulsification device further includes a power assembly 2 , which is disposed in the cavity 11 and located at the upper portion of the cavity 11 .

[0047] The antigen emulsification device further comprises a test tube 3 , which is arranged in the cavity 11 and located at the lower part of the cavity 11 . A sample comprising an antigen and an immune adjuvant is pre-placed in the test tube 3 .

[0048] refer to Figure 1 and Figure 5 The antigen emulsification device further includes a syringe 4 , which includes a barrel 41 , an injection portion 42 , a piston 43 and a push rod 44 .

[0049] The injection part 42 is connected to the barrel 41 and inserted into the test tube 3. The piston 43 is disposed in the barrel 41, and the piston 43 is connected to the push rod 44. The push rod 44 is connected to the power assembly 2, and the push rod 44 is configured to drive the piston 43 to reciprocate under the drive of the power assembly 2, so as to suck the sample in the test tube 3 into the barrel 41 or push the sample in the barrel 41 back into the test tube 3.

[0050] The antigen emulsification device provided in the embodiment of the present disclosure drives the push rod 44 to reciprocate through the power component 2, so that the push rod 44 drives the piston 43 to reciprocate, so as to suck the sample in the test tube 3 into the cylinder 41 or push the sample in the cylinder 41 back into the test tube 3, so that the antigen and immune adjuvant in the test tube 3 are fully emulsified, and the antigen and immune adjuvant in the test tube 3 are automatically injected and pushed by the power component 2, with a high degree of automation, saving labor, and greatly improving the emulsification efficiency. In addition, the power component 2 can provide uniform power, so that the injection and pushing action is more standardized and uniform, thereby improving the antigen emulsification effect, delaying the release of the antigen, prolonging the retention time of the antigen in the body, avoiding frequent injections, and facilitating the production of high-affinity antibodies.

[0051] refer to Figure 1 In some embodiments, the antigen emulsification device further includes a temperature regulating component 5 disposed in the cavity 11 . The temperature regulating component 5 is disposed around the test tube 3 to regulate the temperature of the sample in the test tube 3 .

[0052] The temperature regulating component 5 is arranged around the test tube 3 and can regulate the temperature of the sample in the test tube 3, so as to keep the sample in a low temperature state during the emulsification process, thereby improving the emulsification effect.

[0053] In some embodiments, the temperature adjustment component 5 includes a heat conducting member 52. The heat conducting member 52 is provided with a groove 521 for accommodating the test tube 3, and the groove 521 is configured to be pre-placed with a refrigerant.

[0054] The temperature regulating assembly 5 further includes a semiconductor cooling sheet 51. The semiconductor cooling sheet 51 is disposed at the bottom of the heat conducting member 52 and is configured to reduce the temperature of the refrigerant.

[0055] The semiconductor refrigeration sheet 52 is used to provide cooling, reduce the temperature of the heat conductor 52, and further reduce the temperature of the refrigerant in the groove 521. The test tube 3 is inserted into the groove 521. The temperature of the sample in the test tube 3 is reduced by the refrigerant to provide a low-temperature environment for the sample in the test tube 3, so as to keep the sample in a low-temperature state during the emulsification process and improve the emulsification effect.

[0056] Optionally, the heat conductor 52 includes a copper block.

[0057] In some embodiments, the temperature control component 5 further includes a heat sink 53 , and the heat sink 53 is disposed on a side of the semiconductor cooling sheet 51 away from the heat conducting element 52 .

[0058] In some embodiments, the heat conducting member 52 is located at the lower portion of the cavity 11 in the housing 1 , a semiconductor cooling sheet 51 is disposed at the bottom of the heat conducting member 52 , and a heat dissipating member 53 is disposed on a side of the semiconductor cooling sheet 51 away from the heat conducting member 52 .

[0059] In some embodiments, the power assembly 2 includes a power member 21 and a telescopic member 22. The telescopic member 22 connects the power member 21 and the push rod 44. The power member 21 drives the telescopic member 22 to extend and retract, so that the telescopic member 22 drives the push rod 44 to reciprocate. The push rod 44 drives the piston 43 to reciprocate.

[0060] In some embodiments, the power element 21 includes an electric element.

[0061] In some embodiments, the housing 1 is provided with a connector for charging the electric component or a connector for connecting to a power source.

[0062] refer to Figure 1 , Figure 4 and Figure 5 In some embodiments, the power assembly 2 further includes a housing 23. The housing 23 is hollow, the top of the housing 23 is connected to the top of the cavity 11, the bottom of the housing 23 is open, and a notch 231 is provided at the lower part of the housing 23, the notch 231 is connected to the bottom of the housing 23. A first limiting portion 24 is provided inside the notch 231, and the first limiting portion 24 limits the end edge of the cylinder 41.

[0063] The end edge of the cylinder 41 includes an end outer edge which is arranged at the end of the cylinder 41 and extends outward, and the first limiting portion 24 limits the end outer edge of the cylinder 41 .

[0064] The outer edge of the end of the cylinder 41 and the injection portion 42 are located at two opposite ends of the cylinder 41 respectively.

[0065] In some embodiments, the power assembly 2 further includes a connecting member 25, which is disposed in the housing 23 and connected to the telescopic member 22. The connecting member 25 is provided with a second limiting portion 253, and the second limiting portion 253 limits the end edge of the push rod 44.

[0066] The first end of the push rod 44 is connected to the piston 43 , the end outer edge of the second end of the push rod 44 includes an end outer edge arranged at the second end of the push rod 44 and extending outward, and the second limiting portion 253 limits the end outer edge of the push rod 44 .

[0067] In some embodiments, the first limiting portion 24 includes two circles of bosses 241 that are spaced apart from each other. The bosses 241 extend from the inner wall of the outer shell 23 toward the inside of the outer shell 23 , and the end edge of the cylinder 41 is disposed between the two circles of bosses 241 .

[0068] A clamping groove for clamping the end edge of the cylinder body 41 is formed between the two circles of bosses 241 .

[0069] The end edge of the barrel 41 of the syringe 4 enters into the notch 231 of the housing 23 and is clamped between the two circles of bosses 241 .

[0070] In some embodiments, the connecting member 25 includes a semi-ring portion 251, a closed portion 252 is provided at one end of the semi-ring portion 251, the closed portion 252 is connected to the telescopic member 22, the second limiting portion 253 is provided at the second end of the semi-ring portion 251 and extends toward the interior of the semi-ring portion 251, and the end edge of the push rod 44 is provided between the second limiting portion 253 and the closed portion 252.

[0071] A slot is formed between the second limiting portion 253 and the closing portion 252 , and the outer edge of the end of the push rod 44 enters the slot between the second limiting portion 253 and the closing portion 252 from the semi-annular portion 21 .

[0072] In some embodiments, a partition is further provided in the housing 23, one end of the telescopic member 22 passes through the partition and is connected to the connecting member 25, and the other end of the telescopic member 22 is connected to the power member 21. The partition is used to separate the connecting member 25 from the power member 21.

[0073] In some embodiments, the telescopic member 22 includes a telescopic rod.

[0074] refer to Figure 1 and Figure 3In some embodiments, the antigen emulsification device further includes a control component 6.

[0075] The control component 6 includes a controller, and the controller is electrically connected to the power component 2 .

[0076] The control component 6 includes a display screen 61 , which is electrically connected to the controller and is disposed on the housing 1 . The display screen 61 is provided with buttons for adjusting the emulsification frequency and time.

[0077] In some embodiments, the display screen 61 is located on the top of the housing 1. The bottom of the housing 1 is provided with a plurality of heat dissipation holes.

[0078] refer to Figure 2 In some embodiments, the housing 1 includes a cover plate 12 that can be opened and closed, and an observation window is provided on the cover plate 12.

[0079] During the emulsification process, the cover plate 12 of the housing 1 can maintain the ambient temperature of the test tube 3 .

[0080] In some embodiments, the antigen emulsification device further includes a bracket 7 , which is disposed above the groove 521 and is used to fix the test tube 3 .

[0081] In some embodiments, one, two, three, four, five or more power assemblies 2 are arranged in the housing 1, and each power assembly 2 provides power to a corresponding syringe 4. Each power assembly 2 can work simultaneously or partially. Correspondingly, one, two, three, four, five or more test tubes 3 can be placed in the housing 1. In some embodiments, the antigen emulsification device can emulsify antigens in multiple test tubes 3 at the same time.

[0082] The operation method of the antigen emulsification device provided in the embodiment of the present disclosure is:

[0083] Inject refrigerant into the groove 521 of the heat-conducting member 52 of the temperature-regulating device 5, click the display screen 61 to set the required temperature, and the semiconductor refrigeration plate 51 starts to work. After the temperature of the refrigerant drops to the required temperature, place the test tube 3 pre-filled with antigens and immune adjuvants in the groove 521, and fix the test tube 3 by the bracket 7. Insert the injection part 42 of the syringe 4 into the test tube 3, insert the outer edge of the end of the barrel 41 between the two bosses 214 of the first limit part 24, and insert the outer edge of the end of the push rod 44 between the closing part 252 and the second limit part 253. Click the button on the display screen 61 to set the frequency and time of emulsification. Click the start button on the display screen 61, and drive the push rod 44 to reciprocate through the power assembly 2, so that the push rod 44 drives the piston 43 to reciprocate, so as to suck the sample (antigen and immune adjuvant) in the test tube 3 into the barrel 41 or push the sample in the barrel 41 back into the test tube 3, so that the antigen and immune adjuvant in the test tube 3 are fully emulsified.

[0084] The disclosed embodiment uses the power component 2 to automatically inject and pump the antigen and immune adjuvant in the test tube 3, which has a high degree of automation, saves labor, and greatly improves the emulsification efficiency. The power component 2 can provide uniform power to make the injection and pumping actions more standardized and uniform, which can improve the antigen emulsification effect, delay the release of the antigen, and prolong the retention time of the antigen in the body.

[0085] Based on the above-mentioned embodiments of the present invention, in the absence of explicit negation or conflict, the technical features of one embodiment may be beneficially combined with one or more other embodiments.

[0086] Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It should be understood by those skilled in the art that the above embodiments may be modified or some technical features may be replaced by equivalents without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. An antigen emulsification device, characterized in that: include: A housing (1) having a cavity (11) formed therein; A power assembly (2) is disposed in the cavity (11) and is located at an upper portion of the cavity (11); A test tube (3) is arranged in the cavity (11) and located at the lower part of the cavity (11), wherein a sample including an antigen and an immune adjuvant is pre-placed in the test tube (3); and The syringe (4) comprises a barrel (41), an injection portion (42), a piston (43) and a push rod (44); the injection portion (42) is connected to the barrel (41) and inserted into the test tube (3); the piston (43) is arranged in the barrel (41) and connected to the push rod (44); the push rod (44) is connected to the power assembly (2) and is configured to drive the piston (43) to reciprocate under the drive of the power assembly (2) so as to draw the sample in the test tube (3) into the barrel (41) or push the sample in the barrel (41) back into the test tube (3).

2. The antigen emulsification device according to claim 1, characterized in that: It also comprises a temperature regulating component (5) arranged in the cavity (11); the temperature regulating component (5) is arranged around the test tube (3) to regulate the temperature of the sample in the test tube (3).

3. The antigen emulsification device according to claim 2, characterized in that: The temperature control component (5) comprises a heat conducting member (52) and a semiconductor cooling plate (51); the heat conducting member (52) is provided with a groove (521) for accommodating the test tube (3); the groove (521) is configured to be pre-filled with a refrigerant; the semiconductor cooling plate (51) is provided at the bottom of the heat conducting member (52) and is configured to lower the temperature of the refrigerant.

4. The antigen emulsification device according to claim 3, characterized in that: The temperature adjustment component (5) further comprises a heat sink (53), wherein the heat sink (53) is arranged on a side of the semiconductor cooling plate (51) away from the heat conducting element (52).

5. The antigen emulsification device according to claim 1, characterized in that: The power assembly (2) comprises a power member (21) and a telescopic member (22); the telescopic member (22) connects the power member (21) and the push rod (44); the power member (21) drives the telescopic member (22) to extend and retract, so that the telescopic member (22) drives the push rod (44) to reciprocate.

6. The antigen emulsification device according to claim 5, characterized in that: The power assembly (2) further comprises: A shell (23) is hollow inside, the top of the shell (23) is connected to the top of the cavity (11), the bottom of the shell (23) is open, and a notch (231) is provided at the lower part of the shell (23), a first limiting portion (24) is provided inside the notch (231), and the first limiting portion (24) limits the end edge of the cylinder (41); and A connecting member (25) is disposed in the housing (23) and connected to the telescopic member (22). The connecting member (25) is provided with a second limiting portion (253), and the second limiting portion (253) limits the end edge of the push rod (44).

7. The antigen emulsification device according to claim 6, characterized in that: The first limiting portion (24) comprises two circles of bosses (241) arranged at intervals, the bosses (241) extending from the inner wall of the outer shell (23) toward the inside of the outer shell (23), and the end edge of the cylinder (41) is arranged between the two circles of bosses (241).

8. The antigen emulsification device according to claim 6, characterized in that: The connecting member (25) comprises a semi-ring portion (251), one end of the semi-ring portion (251) is provided with a closed portion (252), the closed portion (252) is connected to the telescopic member (22), the second limiting portion (253) is provided at the second end of the semi-ring portion (251) and extends toward the interior of the semi-ring portion (251), and the end edge of the push rod (44) is provided between the second limiting portion (253) and the closed portion (252).

9. The antigen emulsification device according to claim 1, characterized in that: The device also comprises a control component (6), wherein the control component (6) comprises: A controller electrically connected to the power assembly (2); and A display screen (61) is electrically connected to the controller. The display screen (61) is arranged on the housing (1). The display screen (61) is provided with buttons for adjusting the emulsification frequency and time.

10. The antigen emulsification device according to claim 1, characterized in that: The housing (1) comprises a cover plate (12) which can be opened and closed, and an observation window is provided on the cover plate (12).

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