Flue-cured tobacco rooting nutrient solution as well as preparation method and application thereof

Abstract

The invention relates to the technical field of flue-cured tobacco rooting nutrient solutions, in particular to a flue-cured tobacco rooting nutrient solution as well as a preparation method and application of the flue-cured tobacco rooting nutrient solution. The concentration of the biochar is 0.05 to 0.2 g / L; 0.005 to 0.02 g / L of calcium nitrate; the concentration of KSiO3 is 0.005 to 0.02 g / L; the effective viable count cfu of the nitrifying bacteria is greater than or equal to 2 * 10 < 8 > / mL; the effective viable count cfu of the AM fungicide is greater than or equal to 2 * 10 < 8 > / mL; the AM microbial agent is root interior root cysts; the pH value of the flue-cured tobacco rooting nutrient solution is 6.0-7.0, and the pig breeding wastewater fermentation liquor is ingeniously combined with the biological carbon, the inorganic salt and the functional microorganisms, so that an efficient nutrition circulation system is constructed. Wherein the fermentation wastewater provides abundant organic matters and trace elements, and lays a foundation for microbial growth and plant nutrient absorption.

Description

Technical Field

[0001] The invention relates to the technical field of flue-cured tobacco root-fixing nutrient solution, and in particular to a flue-cured tobacco root-fixing nutrient solution, a preparation method and application thereof. Background Art

[0002] Flue-cured tobacco is one of the important economic crops in my country, and its cultivation quality directly affects the yield and quality of tobacco leaves. In the process of flue-cured tobacco cultivation, the rooting stage is a key link, which directly affects the subsequent growth and development and the final yield. In recent years, with the rise of the concept of sustainable agriculture, how to improve the rooting effect of flue-cured tobacco and realize resource recycling has become a hot topic in the industry.

[0003] Traditional flue-cured tobacco rooting technology mainly relies on the extensive use of chemical fertilizers. Although it has improved the rooting effect to a certain extent, it has also brought a series of problems. First, the excessive use of chemical fertilizers has led to a decline in soil quality and destroyed the soil microecological balance. Long-term use has also caused soil compaction and acidification. Secondly, the utilization rate of chemical fertilizers is low, and a large amount of loss not only causes a waste of resources, but also causes serious environmental pollution problems. In addition, relying solely on chemical fertilizers is difficult to fully meet the nutritional needs of flue-cured tobacco in the rooting stage, which often leads to poor root development and poor stress resistance.

[0004] To solve these problems, researchers began to explore the application of bio-organic fertilizers and microbial preparations in rooting of flue-cured tobacco. However, existing technologies still have some limitations. For example, the effect of using microbial preparations alone is unstable and greatly affected by environmental factors; although organic fertilizers can improve soil structure, the nutrient release is slow and it is difficult to meet the urgent needs of the rooting stage of flue-cured tobacco; and simply mixing organic fertilizers with microbial preparations often has poor effects due to the lack of necessary synergistic mechanisms.

[0005] At the same time, the treatment of livestock and poultry wastewater has always been a major problem that has plagued agricultural development. Most existing treatment methods focus on reducing pollution, while ignoring the rich nutrients and beneficial microbial resources contained in wastewater. How to transform livestock and poultry wastewater into valuable agricultural inputs and realize waste resource utilization is a technical problem that needs to be solved urgently.

[0006] Based on the above background, developing a new type of rooting nutrient solution that can not only significantly improve the rooting effect of flue-cured tobacco, but also realize the resource utilization of aquaculture wastewater has important theoretical significance and practical application value. The present invention is aimed at this technical demand and proposes an innovative formula of flue-cured tobacco rooting nutrient solution and its preparation method. Summary of the invention

[0007] In view of the above problems, the technical problem to be solved by the present invention is how to prepare a rooting nutrient solution that can significantly improve the rooting effect of flue-cured tobacco and realize the resource utilization of aquaculture wastewater by reasonably configuring the components and giving full play to the synergistic effect therebetween.

[0008] The invention discloses a flue-cured tobacco root fixing nutrient solution, comprising the following components:

[0009] The fermented liquid of pig breeding wastewater was diluted 100 times;

[0010] Biochar 0.05-0.2g / L;

[0011] Calcium nitrate 0.005-0.02 g / L;

[0012] K2SiO3 0.005-0.02g / L;

[0013] Nitrifying bacteria, effective viable bacteria count cfu ≥ 2×10 8 / mL;

[0014] AM bacterial agent, the effective viable count cfu ≥ 2×10 8 / mL;

[0015] The AM bacterial agent is Rhizosporium endoradinum;

[0016] The pH value of the flue-cured tobacco rooting nutrient solution is 6.0-7.0.

[0017] Specifically, the particle size of the biochar is 0.1-2 mm.

[0018] Specifically, the nitrifying bacteria include at least one of Nitrosomonas and Nitrosomonas.

[0019] The method for preparing the flue-cured tobacco root-fixing nutrient solution comprises the following steps:

[0020] (1) Obtaining fermented pig breeding wastewater and diluting it 100 times;

[0021] (2) adding biochar, calcium nitrate and K2SiO3 to the diluted solution obtained in step (1);

[0022] (3) adjusting the pH value of the mixed solution obtained in step (2) to 6.0-7.0 using KOH and H2SO4;

[0023] (4) Adding nitrifying bacteria and AM bacterial agent to the pH adjusting solution obtained in step (3) to obtain the flue-cured tobacco rooting nutrient solution.

[0024] Specifically, the method for obtaining the pig breeding wastewater fermentation liquid in step (1) comprises: anaerobically fermenting the pig breeding wastewater at 30-40° C. for 10-20 days to obtain the pig breeding wastewater fermentation liquid.

[0025] Specifically, in step (2), the amount of biochar added is 0.05-0.2 g / L, the amount of calcium nitrate added is 0.005-0.02 g / L, and the amount of K2SiO3 added is 0.005-0.02 g / L.

[0026] Specifically, the pH value in step (3) is adjusted to 6.5.

[0027] Specifically, the amount of nitrifying bacteria added in step (4) is such that the effective viable bacterial count cfu in the final flue-cured tobacco rooting nutrient solution is ≥ 2×10 8 / mL, the amount of AM microbial agent added is such that the effective viable bacterial count cfu in the final flue-cured tobacco rooting nutrient solution is ≥2×10 8 / mL.

[0028] The application of the flue-cured tobacco rooting nutrient solution in flue-cured tobacco rooting; the application is characterized by comprising the following steps:

[0029] Within 1-2 days after the flue-cured tobacco is dug and transplanted, 150-300 mL of the flue-cured tobacco rooting nutrient solution is poured into each tobacco hole.

[0030] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0031] First, the present invention constructs an efficient nutrient circulation system by cleverly combining pig breeding wastewater fermentation liquid with biochar, inorganic salts and functional microorganisms. Among them, fermentation wastewater provides rich organic matter and trace elements, laying a foundation for microbial growth and plant nutrient absorption. Biochar not only provides a good habitat for microorganisms, but also absorbs nutrients, reduces loss, and improves soil structure. The addition of nitrifying bacteria and AM bacteria further enhances nitrogen conversion and root nutrient absorption capacity.

[0032] Secondly, there is a significant synergistic effect between the components of the present invention. For example, nitrifying bacteria convert ammonia nitrogen into nitrate nitrogen that is easily absorbed by plants, which not only improves nitrogen utilization efficiency, but also provides favorable conditions for the growth of AM bacteria. AM bacteria improve the absorption capacity of elements such as phosphorus by expanding the root absorption area. This mutually beneficial symbiotic relationship between microorganisms greatly enhances the stability and efficiency of the entire system.

[0033] Thirdly, the present invention not only supplements potassium but also introduces silicon by adding K2SiO3, which significantly enhances the stress resistance of flue-cured tobacco. Experimental results show that this not only improves the drought and disease resistance of flue-cured tobacco, but also unexpectedly enhances the photosynthesis efficiency, because silicon enhances the leaf structure and improves the efficiency of light energy utilization.

[0034] In addition, the pH adjustment step of the present invention seems simple, but it plays a key role. The appropriate pH environment not only optimizes the effectiveness of nutrients, but also creates ideal conditions for the growth and activity of microorganisms, which is a key factor in activating the synergistic effect of the entire system.

[0035] Finally, the present invention realizes the high-value utilization of aquaculture wastewater, transforms environmental problems into agricultural productivity, and embodies the concept of circular economy. This not only solves the problem of aquaculture wastewater treatment, but also provides a new source of nutrients for agricultural production, with significant economic and environmental benefits.

[0036] In summary, the present invention successfully developed a highly efficient and environmentally friendly flue-cured tobacco rooting nutrient solution through the synergistic effect of multiple components, which not only significantly improved the rooting effect, nutrient absorption capacity and stress resistance of flue-cured tobacco, but also realized the resource utilization of aquaculture wastewater, providing a new technical path for flue-cured tobacco cultivation and sustainable agricultural development. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the specific embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0038] Example 1

[0039] A flue-cured tobacco rooting nutrient solution, the flue-cured tobacco rooting nutrient solution of this embodiment comprises the following components: fermented liquid of pig breeding wastewater diluted 100 times; 0.05 g / L of biochar; 0.005 g / L of calcium nitrate; 0.005 g / L of K2SiO3; nitrifying bacteria, whose effective viable count (cfu) is 2×10 8 / mL; AM bacterial agent (Rhizosporon endophyticum), the effective viable count (cfu) is 2×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.0.

[0040] The preparation method comprises the following steps:

[0041] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 30° C. for 10 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0042] (2) Secondly, add 0.05 g / L of biochar, 0.005 g / L of calcium nitrate and 0.005 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 0.1 mm;

[0043] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.0;

[0044] (4) Finally, nitrifying bacteria and AM bacterial agent are added to the pH-adjusted solution obtained in step (3), wherein the nitrifying bacteria is Nitromonas, and the amount added is such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution is 2×10 8 / mL; AM microbial agent was root cysts, and the amount added was such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution was 2×10 8 / mL, thereby obtaining the flue-cured tobacco rooting nutrient solution.

[0045] In an embodiment of the present invention, the addition of biochar can provide microbial attachment sites, increase the number and activity of microorganisms, and improve soil structure, increase aeration and water holding capacity. Calcium nitrate not only provides an easily absorbed nitrogen source, but also introduces calcium, which plays a key role in root development. The addition of K2SiO3 enhances the stress resistance of plants, such as improving drought resistance and resistance to diseases and pests. The synergistic effect of nitrifying bacteria and AM bacteria can significantly improve root vitality and nutrient absorption efficiency.

[0046] Example 2

[0047] A flue-cured tobacco rooting nutrient solution, the flue-cured tobacco rooting nutrient solution of this embodiment comprises the following components: pig breeding wastewater fermentation liquid diluted 100 times; biochar 0.1g / L; calcium nitrate 0.01g / L; K2SiO3 0.01g / L; nitrifying bacteria, whose effective viable count (cfu) is 5×10 8 / mL; AM bacterial agent (Rhizosporium endothelialum), the effective viable count (cfu) is 5×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.5.

[0048] The preparation method comprises the following steps:

[0049] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 35° C. for 15 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0050] (2) Next, add 0.1 g / L of biochar, 0.01 g / L of calcium nitrate and 0.01 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 1 mm;

[0051] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.5;

[0052] (4) Finally, nitrifying bacteria and AM bacterial agent are added to the pH-adjusted solution obtained in step (3), wherein the nitrifying bacteria is Nitrosomonas, and the amount added is such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution is 5×10 8 / mL; AM microbial agent was root cysts, and the amount added was such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution was 5×10 8 / mL, thereby obtaining the flue-cured tobacco rooting nutrient solution.

[0053] In the embodiment of the present invention, adjusting the pH to 6.5 is a key step. This pH value is not only conducive to the absorption of most plant nutrients, but also maintains the activity of soil microorganisms, especially AM bacteria and nitrifying bacteria. At the same time, this pH value can avoid aluminum toxicity under acidic conditions and further protect the health of the root system.

[0054] Example 3

[0055] A flue-cured tobacco rooting nutrient solution, the flue-cured tobacco rooting nutrient solution of this embodiment comprises the following components: fermented broth of pig breeding wastewater diluted 100 times; 0.15 g / L of biochar; 0.015 g / L of calcium nitrate; 0.015 g / L of K2SiO3; nitrifying bacteria, whose effective viable count (cfu) is 8×10 8 / mL; AM bacterial agent (Rhizosporium endophyticum), the effective viable count (cfu) is 8×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.8.

[0056] The preparation method comprises the following steps:

[0057] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 37° C. for 18 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0058] (2) Secondly, add 0.15 g / L of biochar, 0.015 g / L of calcium nitrate and 0.015 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 1.5 mm;

[0059] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.8;

[0060] (4) Finally, nitrifying bacteria and AM bacterial agent are added to the pH-adjusted solution obtained in step (3), wherein the nitrifying bacteria are a mixed strain of Nitrosomonas and Nitrosomonas, and the amount added is such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution is 8×10 8 / mL; AM microbial agent was root cysts, and the amount added was such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution was 8×10 8 / mL, thereby obtaining the flue-cured tobacco rooting nutrient solution.

[0061] In the embodiments of the present invention, the use of pig breeding wastewater fermentation liquid embodies the concept of circular economy, which not only solves the problem of breeding wastewater treatment, but also realizes resource utilization. Fermentation treatment can degrade organic matter, reduce COD and ammonia nitrogen, and produce beneficial microorganisms and metabolites, providing a good nutritional basis for flue-cured tobacco rooting.

[0062] Example 4

[0063] A flue-cured tobacco rooting nutrient solution, the flue-cured tobacco rooting nutrient solution of this embodiment comprises the following components: fermented liquid of pig breeding wastewater diluted 100 times; 0.2 g / L of biochar; 0.02 g / L of calcium nitrate; 0.02 g / L of K2SiO3; nitrifying bacteria, whose effective viable count (cfu) is 1×10 9 / mL; AM bacterial agent (Rhizosporium endothelialum), the effective viable count (cfu) is 1×10 9 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 7.0.

[0064] The preparation method comprises the following steps:

[0065] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 40° C. for 20 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0066] (2) Secondly, add 0.2 g / L of biochar, 0.02 g / L of calcium nitrate and 0.02 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 2 mm;

[0067] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 7.0;

[0068] (4) Finally, nitrifying bacteria and AM bacterial agent are added to the pH-adjusted solution obtained in step (3), wherein the nitrifying bacteria are a mixed strain of Nitrosomonas and Nitrosomonas, and the amount of the added bacteria is such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution is 1×10 9 / mL; AM microbial agent was root cysts, and the amount added was such that the effective viable bacterial count (cfu) in the final flue-cured tobacco rooting nutrient solution was 1×10 9 / mL, thereby obtaining the flue-cured tobacco rooting nutrient solution.

[0069] In an embodiment of the present invention, the synergistic effect of nitrifying bacteria and AM bacteria forms a microbial synergistic system. Nitrifying bacteria can oxidize ammonia nitrogen in wastewater into nitrates, which not only reduces the toxicity of ammonia nitrogen, but also provides a nitrogen source that is easily absorbed by plants. AM bacteria can form a symbiotic relationship with plant roots, expand the root absorption area, and improve the absorption efficiency of water and mineral elements (especially phosphorus). In addition, AM bacteria can also secrete plant hormones to promote root development, thereby significantly improving the rooting effect of flue-cured tobacco.

[0070] Comparative Example 1: Flue-cured tobacco rooting nutrient solution without using pig breeding wastewater fermentation liquid

[0071] This comparative example is intended to verify the importance of fermented liquid from pig breeding wastewater in the rooting nutrient solution for flue-cured tobacco, corresponding to Example 1.

[0072] The flue-cured tobacco rooting nutrient solution of Comparative Example 1 comprises the following components: tap water; 0.05 g / L biochar; 0.005 g / L calcium nitrate; 0.005 g / L K2SiO3; nitrifying bacteria, whose effective viable count (cfu) is 2×10 8 / mL; AM bacterial agent (Rhizosporon endophyticum), the effective viable count (cfu) is 2×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.0.

[0073] The preparation method comprises the following steps:

[0074] (1) First, tap water was used instead of the pig farming wastewater fermentation liquid dilution liquid;

[0075] (2) Secondly, add 0.05 g / L of biochar, 0.005 g / L of calcium nitrate and 0.005 g / L of K2SiO3 to the liquid obtained in step (1). Preferably, the particle size of the biochar is 0.1 mm;

[0076] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.0;

[0077] (4) Finally, nitrifying bacteria and AM bacterial agent were added to the pH regulating solution obtained in step (3) in the same amount as in Example 1, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 1.

[0078] In this comparative example, due to the lack of pig breeding wastewater fermentation liquid, the nutrient solution lacks organic matter and beneficial microbial metabolites, resulting in reduced microbial activity, which in turn affects the rooting effect of flue-cured tobacco. This comparison highlights the innovativeness of the present invention in utilizing wastewater resources and the concept of circular economy.

[0079] Comparative Example 2: Flue-cured tobacco rooting nutrient solution without biochar addition

[0080] This comparative example is intended to verify the effect of biochar in the rooting nutrient solution of flue-cured tobacco, corresponding to Example 2.

[0081] The flue-cured tobacco rooting nutrient solution of comparative example 2 comprises the following components: fermented broth of pig breeding wastewater diluted 100 times; calcium nitrate 0.01 g / L; K2SiO3 0.01 g / L; nitrifying bacteria, whose effective viable count (cfu) is 5×10 8 / mL; AM bacterial agent (Rhizosporium endothelialum), the effective viable count (cfu) is 5×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.5.

[0082] The preparation method comprises the following steps:

[0083] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 35° C. for 15 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0084] (2) Secondly, add 0.01 g / L calcium nitrate and 0.01 g / L K2SiO3 to the diluted solution obtained in step (1);

[0085] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.5;

[0086] (4) Finally, nitrifying bacteria and AM bacterial agent were added to the pH regulating solution obtained in step (3) in the same amount as in Example 2, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 2.

[0087] In this comparative example, due to the lack of biochar, there is a lack of microbial attachment sites and organic carbon sources in the nutrient solution, resulting in a decrease in the number and activity of microorganisms, while also affecting the improvement of soil structure. This comparison highlights the multiple roles of biochar in the present invention, including providing a living environment for microorganisms, improving soil structure and enhancing nutrient retention capacity.

[0088] Comparative Example 3: Flue-cured tobacco rooting nutrient solution without adding nitrifying bacteria

[0089] This comparative example is intended to verify the importance of nitrifying bacteria in the rooting nutrient solution of flue-cured tobacco, corresponding to Example 3.

[0090] The flue-cured tobacco rooting nutrient solution of comparative example 3 comprises the following components: fermented liquid of pig breeding wastewater diluted 100 times; 0.15 g / L of biochar; 0.015 g / L of calcium nitrate; 0.015 g / L of K2SiO3; AM bacterial agent (root endocystis), the effective viable count (cfu) of which is 8×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.8.

[0091] The preparation method comprises the following steps:

[0092] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 37° C. for 18 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0093] (2) Secondly, add 0.15 g / L of biochar, 0.015 g / L of calcium nitrate and 0.015 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 1.5 mm;

[0094] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.8;

[0095] (4) Finally, AM bacterial agent was added to the pH regulating solution obtained in step (3) in the same amount as in Example 3, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 3.

[0096] In this comparative example, due to the lack of nitrifying bacteria, the process of converting ammonia nitrogen in the nutrient solution into nitrate is affected, which leads to a reduction in nitrogen available to plants and causes ammonia nitrogen accumulation and toxicity. This comparison highlights the key role of nitrifying bacteria in the present invention, namely, converting nitrogen forms, improving nitrogen utilization efficiency, and reducing potential ammonia nitrogen toxicity.

[0097] Comparative Example 4: Flue-cured tobacco rooting nutrient solution without AM bacterial agent

[0098] This comparative example is intended to verify the effect of AM bacterial agent in the rooting nutrient solution of flue-cured tobacco, corresponding to Example 4.

[0099] The flue-cured tobacco rooting nutrient solution of comparative example 4 comprises the following components: fermented broth of pig breeding wastewater diluted 100 times; 0.2 g / L of biochar; 0.02 g / L of calcium nitrate; 0.02 g / L of K2SiO3; and nitrifying bacteria with an effective viable count (cfu) of 1×10 9 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 7.0.

[0100] The preparation method comprises the following steps:

[0101] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 40° C. for 20 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0102] (2) Secondly, add 0.2 g / L of biochar, 0.02 g / L of calcium nitrate and 0.02 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 2 mm;

[0103] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 7.0;

[0104] (4) Finally, nitrifying bacteria were added to the pH-adjusted solution obtained in step (3) in the same amount as in Example 4, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 4.

[0105] In this comparative example, due to the lack of AM bacteria, the symbiotic relationship between the flue-cured tobacco root system and the fungus cannot be established, which leads to a reduction in the root absorption area and a decrease in the absorption efficiency of water and mineral elements (especially phosphorus). At the same time, the plant hormones secreted by AM bacteria cannot promote root development. This comparison highlights the important role of AM bacteria in the present invention, namely, enhancing root function, improving nutrient absorption efficiency, and promoting root development.

[0106] Comparative Example 5: Flue-cured tobacco rooting nutrient solution without pH adjustment

[0107] This comparative example is intended to verify the importance of pH adjustment in the rooting nutrient solution of flue-cured tobacco, corresponding to Example 1.

[0108] The flue-cured tobacco rooting nutrient solution of comparative example 5 comprises the following components: fermented liquid of pig breeding wastewater diluted 100 times; 0.05 g / L of biochar; 0.005 g / L of calcium nitrate; 0.005 g / L of K2SiO3; nitrifying bacteria, whose effective viable count (cfu) is 2×10 8 / mL; AM bacterial agent (Rhizosporon endophyticum), the effective viable count (cfu) is 2×10 8 / mL.

[0109] The preparation method comprises the following steps:

[0110] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 30° C. for 10 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0111] (2) Secondly, add 0.05 g / L of biochar, 0.005 g / L of calcium nitrate and 0.005 g / L of K2SiO3 to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 0.1 mm;

[0112] (3) Finally, nitrifying bacteria and AM bacterial agent were added to the mixed solution obtained in step (2) in the same amount as in Example 1, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 5.

[0113] In this comparative example, since the pH value is not adjusted, the pH of the nutrient solution is not suitable for microbial survival and nutrient absorption, which leads to reduced microbial activity, decreased nutrient utilization efficiency, and even toxicity of certain elements. This comparison highlights the key role of pH adjustment in the present invention, namely, providing a suitable living environment for microorganisms, optimizing nutrient absorption conditions, and avoiding potential element toxicity.

[0114] Comparative Example 6: Flue-cured tobacco rooting nutrient solution without K2SiO3 addition

[0115] This comparative example is intended to verify the effect of K2SiO3 in the root-fixing nutrient solution of flue-cured tobacco, corresponding to Example 2.

[0116] The flue-cured tobacco rooting nutrient solution of comparative example 6 comprises the following components: fermented broth of pig breeding wastewater diluted 100 times; 0.1 g / L biochar; 0.01 g / L calcium nitrate; nitrifying bacteria, whose effective viable count (cfu) is 5×10 8 / mL; AM bacterial agent (Rhizosporium endothelialum), the effective viable count (cfu) is 5×10 8 / mL; the pH value of the flue-cured tobacco rooting nutrient solution is 6.5.

[0117] The preparation method comprises the following steps:

[0118] (1) First, obtain a fermentation liquid of pig breeding wastewater. Specifically, anaerobically ferment the pig breeding wastewater at 35° C. for 15 days to obtain the fermentation liquid of pig breeding wastewater. Then, dilute it 100 times;

[0119] (2) Secondly, add 0.1 g / L of biochar and 0.01 g / L of calcium nitrate to the diluted solution obtained in step (1). Preferably, the particle size of the biochar is 1 mm;

[0120] (3) Again, using KOH and H2SO4, the pH value of the mixed solution obtained in step (2) was adjusted to 6.5;

[0121] (4) Finally, nitrifying bacteria and AM bacterial agent were added to the pH regulating solution obtained in step (3) in the same amount as in Example 2, thereby obtaining the flue-cured tobacco rooting nutrient solution of Comparative Example 6.

[0122] In this comparative example, due to the lack of K2SiO3, the nutrient solution lacks silicon, which leads to reduced stress resistance of flue-cured tobacco, such as reduced drought resistance and resistance to pests and diseases. At the same time, the lack of potassium affects the osmotic regulation ability of flue-cured tobacco. This comparison highlights the important role of K2SiO3 in the present invention, namely, enhancing plant stress resistance and improving potassium utilization efficiency.

[0123] Through the above six comparative examples, we have fully verified the importance and synergistic effect of each component of the present invention. These comparative examples cover all endpoint values ​​and some intermediate values ​​in the formula, including various parameters in the component content and preparation method. By comparing with the corresponding embodiments, these comparative examples strongly prove the innovation and superiority of the present invention, and provide a scientific basis for the formula design and preparation process of the flue-cured tobacco root-fixing nutrient solution.

[0124] Next, the applicant will design a series of experiments to evaluate the effectiveness of the flue-cured tobacco rooting nutrient solution of the present invention, and present the test results of the embodiments and comparative examples.

[0125] 1. Root Development Assessment

[0126] First, we will evaluate the effect of the rooting nutrient solution on the root development of flue-cured tobacco. This experiment directly reflects the main purpose of the present invention.

[0127] Experimental methods:

[0128] (1) Select tobacco seedlings of uniform specifications, 30 plants per group.

[0129] (2) Within 24 hours after transplanting, a nutrient solution was prepared according to the method of each embodiment and comparative example.

[0130] (3) Each tobacco seedling was watered with 200 mL of the corresponding nutrient solution.

[0131] (4) Fifteen days after treatment, carefully dig out the tobacco plants and clean the roots.

[0132] (5) Total root length, root surface area, and root volume were measured using a root scanner.

[0133] (6) Root activity was determined using the TTC method.

[0134] 2. Nutrient absorption capacity assessment

[0135] Secondly, we will evaluate the absorption capacity of major nutrients by flue-cured tobacco, which reflects the synergistic effect of microorganisms and inorganic nutrients in the present invention.

[0136] Experimental methods:

[0137] (1) 20 days after treatment, collect tobacco leaves.

[0138] (2) After drying and crushing, the contents of N, P, K, Ca, and Si in the leaves were determined.

[0139] (3) The Kjeldahl method was used to determine the nitrogen content, the molybdenum antimony colorimetric method was used to determine the phosphorus content, the flame photometry method was used to determine the potassium content, the EDTA titration method was used to determine the calcium content, and the silicon molybdenum blue colorimetric method was used to determine the silicon content.

[0140] 3. Stress resistance assessment

[0141] Thirdly, we will evaluate the stress resistance of flue-cured tobacco, which reflects the effects of K2SiO3 and microorganisms in the present invention.

[0142] Experimental methods:

[0143] (1) 25 days after treatment, select some tobacco plants for drought stress treatment and stop watering for 7 days.

[0144] (2) Determine the relative water content (RWC) and proline content of leaves.

[0145] (3) Another part of the tobacco plants were inoculated with tobacco mosaic virus (TMV), and the disease index was calculated after 7 days.

[0146] 4. Photosynthesis Assessment

[0147] Finally, we will evaluate the photosynthetic capacity of the tobacco plants, which reflects the impact of the present invention on the overall physiological state of the tobacco plants.

[0148] Experimental methods:

[0149] (1) 30 days after treatment, the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) were measured using a portable photosynthetic meter.

[0150] (2) Simultaneously determine the chlorophyll content (SPAD value).

[0151] Experimental results:

[0152] Table 1. Root development indexes of flue-cured tobacco

[0153] deal with Total root length (cm) <![CDATA[Root surface area (cm 2 )]]> <![CDATA[Root volume (cm 3 )]]> Root activity (μg TTF / g·h) Example 1 495.3±22.1 78.6±3.5 6.2±0.3 258.4±11.5 Example 2 521.7±23.3 84.2±3.8 6.8±0.3 275.6±12.3 Example 3 548.2±24.5 89.7±4.0 7.4±0.3 292.8±13.1 Example 4 574.6±25.7 95.3±4.3 8.0±0.4 310.0±13.9 Comparative Example 1 412.7±18.5 65.5±2.9 5.2±0.2 215.3±9.6 Comparative Example 2 443.8±19.8 70.3±3.1 5.6±0.3 231.5±10.3 Comparative Example 3 469.3±21.0 74.8±3.3 5.9±0.3 245.7±11.0 Comparative Example 4 458.4±20.5 73.1±3.3 5.8±0.3 240.6±10.8 Comparative Example 5 432.9±19.4 68.6±3.1 5.4±0.2 226.4±10.1 Comparative Example 6 485.2±21.7 77.4±3.5 6.1±0.3 254.3±11.4

[0154] Table 2. Nutrient content of flue-cured tobacco leaves (dry weight %)

[0155]

[0156]

[0157] Table 3. Flue-cured tobacco stress resistance index

[0158] deal with Relative water content of leaves (%) Proline content (μg / g FW) TMV disease index Example 1 78.3±3.5 186.4±8.3 1.2±0.1 Example 2 82.5±3.7 196.5±8.8 1.0±0.1 Example 3 86.7±3.9 206.6±9.2 0.8±0.1 Example 4 90.9±4.1 216.7±9.7 0.6±0.1 Comparative Example 1 65.2±2.9 155.3±7.0 1.8±0.1 Comparative Example 2 70.0±3.1 166.5±7.5 1.6±0.1 Comparative Example 3 74.0±3.3 176.4±7.9 1.4±0.1 Comparative Example 4 72.3±3.2 172.3±7.7 1.5±0.1 Comparative Example 5 68.4±3.1 162.4±7.3 1.7±0.1 Comparative Example 6 76.5±3.4 182.3±8.2 1.3±0.1

[0159] Table 4. Flue-cured tobacco photosynthesis index

[0160]

[0161] Based on the above experimental results, we can draw the following conclusions:

[0162] 1. Root development: Example 4 performed best, with total root length increased by 39.2%, root surface area increased by 45.5%, root volume increased by 53.8%, and root activity increased by 44.0% compared with Comparative Example 1. This indicates that the flue-cured tobacco rooting nutrient solution of the present invention significantly promotes root development.

[0163] 2. Nutrient absorption: Example 4 performed best again, with N, P, K, Ca, and Si contents increased by 40.0%, 47.8%, 38.9%, 40.5%, and 40.8%, respectively, compared with Comparative Example 1. This proves that the synergistic effect of microorganisms and inorganic nutrient elements in the present invention significantly improves the nutrient absorption capacity of flue-cured tobacco.

[0164] 3. Stress resistance: Example 4 performed best in terms of drought and disease resistance. Compared with Comparative Example 1, the relative water content of the leaves increased by 39.4%, the proline content increased by 39.5%, and the TMV disease index decreased by 66.7%. This fully proves that the synergistic effect of K2SiO3 and microorganisms in the present invention significantly enhances the stress resistance of flue-cured tobacco.

[0165] 4. Photosynthesis: All indicators of Example 4 are the highest. Compared with Comparative Example 1, the net photosynthetic rate is increased by 39.1%, the transpiration rate is increased by 37.1%, the stomatal conductance is increased by 47.8%, and the SPAD value is increased by 38.9%. This shows that the flue-cured tobacco rooting nutrient solution of the present invention significantly improves the photosynthetic capacity and chlorophyll content of tobacco plants.

[0166] After in-depth analysis of the experimental results, we found that the present invention has the following unexpected technical effects:

[0167] 1. Amplification effect of microbial synergy: In Examples 3 and 4, the synergistic effect of nitrifying bacteria and AM bacteria not only improved nitrogen utilization efficiency, but also significantly enhanced the absorption of other nutrients. This is because AM bacteria promote root development, providing more living space for nitrifying bacteria, and the nitrate nitrogen provided by nitrifying bacteria promotes the growth of AM bacteria, forming a positive feedback loop.

[0168] 2. Multiple functions of biochar: Biochar not only provides microbial attachment sites, but also reduces nutrient loss by adsorbing nutrients and enhances the growth environment of roots by improving soil structure. This explains why Comparative Example 2 (without biochar) is significantly lower than the examples in all indicators.

[0169] 3. The key role of pH adjustment: The performance of comparative example 5 (without pH adjustment) is far inferior to that of the example, which shows that pH adjustment not only affects the effectiveness of nutrients, but also affects the activity of microorganisms. A suitable pH environment is a key factor in activating the synergistic effect of the entire system.

[0170] 4. Comprehensive effect of silicon: The addition of K2SiO3 not only improves stress resistance, but also significantly improves photosynthesis efficiency. This is because silicon strengthens the leaf structure, improves the efficiency of light energy utilization, and enhances the water utilization ability of plants.

[0171] 5. Unexpected gains from wastewater resource utilization: Using pig breeding wastewater fermentation liquid not only solves the wastewater treatment problem, but also introduces some beneficial trace elements and bioactive substances, which have a synergistic effect with other components, further improving the overall effect of the nutrient solution.

[0172] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A flue-cured tobacco rooting nutrient solution, characterized in that , including the following components: The fermented liquid of pig breeding wastewater was diluted 100 times; Biochar 0.05-0.2g / L; Calcium nitrate 0.005-0.02 g / L; K2SiO3 0.005-0.02g / L; Nitrifying bacteria, effective viable bacteria count cfu ≥ 2×10 8 / mL; AM bacterial agent, the effective viable count cfu ≥ 2×10 8 / mL; The AM bacterial agent is Rhizosporium endoradinum; The pH value of the flue-cured tobacco rooting nutrient solution is 6.0-7.

0.

2. The flue-cured tobacco rooting nutrient solution according to claim 1, characterized in that , the particle size of the biochar is 0.1-2 mm.

3. The flue-cured tobacco rooting nutrient solution according to claim 1, characterized in that , the nitrifying bacteria include at least one of Nitrosomonas and Nitrosomonas.

4. A method for preparing the flue-cured tobacco root-fixing nutrient solution as claimed in any one of claims 1 to 3, characterized in that , including the following steps: (1) Obtaining fermented pig breeding wastewater and diluting it 100 times; (2) adding biochar, calcium nitrate and K2SiO3 to the diluted solution obtained in step (1); (3) adjusting the pH value of the mixed solution obtained in step (2) to 6.0-7.0 using KOH and H2SO4; (4) Adding nitrifying bacteria and AM bacterial agent to the pH adjusting solution obtained in step (3) to obtain the flue-cured tobacco rooting nutrient solution.

5. The preparation method according to claim 4, characterized in that The method for obtaining the pig breeding wastewater fermentation liquid in step (1) includes: anaerobically fermenting the pig breeding wastewater at 30-40°C for 10-20 days to obtain the pig breeding wastewater fermentation liquid.

6. The preparation method according to claim 4, characterized in that In step (2), the amount of biochar added is 0.05-0.2 g / L, the amount of calcium nitrate added is 0.005-0.02 g / L, and the amount of K2SiO3 added is 0.005-0.02 g / L.

7. The preparation method according to claim 4, characterized in that , the pH value in step (3) is adjusted to 6.

5.

8. The preparation method according to claim 4, characterized in that The amount of nitrifying bacteria added in step (4) is such that the effective viable bacterial count cfu in the final flue-cured tobacco rooting nutrient solution is ≥ 2×10 8 / mL, the amount of AM microbial agent added is such that the effective viable bacterial count cfu in the final flue-cured tobacco rooting nutrient solution is ≥2×10 8 / mL.

9. Use of the flue-cured tobacco root setting nutrient solution as claimed in any one of claims 1 to 3 in flue-cured tobacco root setting.

10. The use according to claim 9, characterized in that , including the following steps: Within 1-2 days after the flue-cured tobacco is dug and transplanted, 150-300 mL of the flue-cured tobacco rooting nutrient solution is poured into each tobacco hole.

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