Processing method of characteristic Chinese gall leaven of fructus evodiae

Abstract

The invention belongs to the technical field of Chinese gall leaven, and particularly relates to a processing method of Wu characteristic Chinese gall leaven. The processing method comprises the following steps: dispersing nano-particles in a coating material to obtain a nano-material coating solution; putting the new tea into the nano-material coating solution, fully soaking, and drying after coating to obtain coated tea leaves; the coated tea leaves are subjected to illumination, and the illuminated tea leaves are obtained; aging the illuminated tea leaves to obtain aged tea leaves; taking the aged tea leaves, adding water to decoct for several times, after decoction is completed, combining filtrate, filtering to obtain clear filtrate, cooling, adding vinasse and gallnut powder to obtain a mixture, adding water, stirring with the mixture to form soft blocks, fermenting, taking out when white hair is spread, drying, and cutting into blocks to obtain the Chinese gall leaven. The method can improve the content of index components of the Chinese gall leaven.

Description

Technical Field

[0001] The invention belongs to the technical field of Baiyaojian, and particularly relates to a preparation method of Wumen characteristic Baiyaojian. Background Art

[0002] Ye Tianshi of the Wumen School of Medicine proposed for the first time in "Treatise on Warm and Hot Diseases" the etiology of warm diseases that "warm evil is received from above, first attacks the lungs and then spreads to the pericardium". He also pioneered the "Wei, Qi, Ying, and Blood" syndrome differentiation outline for warm diseases and the treatment methods such as relieving exterior symptoms with pungent and cool herbs, nourishing yin with sweet and cold herbs, clearing away heat and detoxifying, and nourishing yin and protecting body fluids.

[0003] Baiyaojian can be used to treat febrile diseases. Baiyaojian is sour, sweet and neutral. It enters the lung and stomach meridians. It clears away heat and resolves phlegm, promotes the production of body fluids and quenches thirst. It is mainly used to treat and regulate the respiratory and digestive systems, such as cough due to lung heat, toothache due to wind and fire, oral erosion, and rectal prolapse due to chronic dysentery.

[0004] Baiyaojian is a dried block made by fermenting gallnut, tea, distiller's grains or distiller's yeast. The main indicator component of Baiyaojian is gallic acid, which is converted from the hydrolyzable tannin in gallnut through microbial fermentation. Therefore, by measuring the gallic acid content in Baiyaojian, the quality of the drug efficacy can be preliminarily judged.

[0005] Therefore, the research on Baiyaojian usually focuses on how to increase the gallic acid content of Baiyaojian. For example, the patent publication number CN103893392B discloses a method for increasing the gallic acid content in Baiyaojian, which includes the following steps: mixing tea juice and crushed distiller's grains (distiller's yeast) into a solution, adding gallnut powder to the solution, and mixing to obtain a solution; culturing the solution to obtain a microbial seed solution; mixing the microbial seed solution and gallnut powder, granulating, culturing, and drying after uniform mixing. It utilizes all the beneficial microorganisms in the distiller's grains for synergistic fermentation, and preserves the medicinal properties of traditional Baiyaojian to the greatest extent. The gallnut added to the culture medium can inhibit the growth of non-effective microorganisms and effectively increase the content of gallic acid.

[0006] In the prior art, including the aforementioned patent, scholars have always studied the technical problem of "how to increase the content of the index components of Baiyaojian" along the route of optimizing the fermentation of koji and gallnut. Different from the prior art, this application attempts to provide a new route to solve the aforementioned technical problem. Summary of the invention

[0007] The purpose of the present invention is to provide a preparation method of Wumen characteristic Baiyaojian, and the present invention improves the content of index components of Baiyaojian from different routes.

[0008] In order to achieve the above-mentioned object and other related objects, the present invention provides the following technical solutions:

[0009] In a first aspect, the present invention provides a method for preparing Wumen Characteristic Baiyao Decoction, comprising the following steps:

[0010] dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution;

[0011] Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves;

[0012] Exposing the coated tea leaves to light to obtain illuminated tea leaves;

[0013] Aging the tea leaves after being exposed to light to obtain aged tea leaves;

[0014] Take aged tea leaves, add water to them in batches and boil them. After boiling, combine the filtrates, filter them to obtain clarified filtrates, cool them, add distiller's grains and gallnut powder to obtain a mixture, then add water to the mixture and stir it into soft blocks. Ferment the mixture until it is covered with white hairs, take it out, dry it and cut it into blocks to obtain Baiyaojian.

[0015] In one embodiment of the present invention, the nanoparticles include at least one of nano zinc oxide and nano titanium dioxide.

[0016] In one embodiment of the present invention, the coating material includes chitosan solution.

[0017] In one embodiment of the present invention, the steps of preparing the nanomaterial coating solution include:

[0018] Dissolve chitosan in acetic acid solution with a concentration of 2% to 5%, and stir until completely dissolved to obtain a chitosan solution with a concentration of 2% to 5%;

[0019] The nanoparticles are placed in a chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency is set to 10 to 30 kHz and the dispersion time is 10 to 50 minutes to obtain a nanomaterial coating solution with a concentration of 0.01% to 1%.

[0020] It should be noted that chitosan forms a thin film after dissolving, which can effectively coat nanoparticles and improve the stability and dispersibility of nanoparticles. This film not only protects the nanoparticles, but also prevents the agglomeration of nanoparticles, ensuring that the nanoparticles can be evenly distributed on the surface of the tea leaves when coating new tea.

[0021] In one embodiment of the present invention, the steps of preparing the nanomaterial coating solution include:

[0022] Dissolve chitosan in acetic acid solution with a concentration of 2% to 5%, and stir until completely dissolved to obtain a chitosan solution with a concentration of 2% to 5%;

[0023] Nano zinc oxide and nano titanium dioxide are placed in a chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency is set to 10-30 kHz and the dispersion time is 10-50 minutes to obtain a nano material coating solution with a concentration of 0.01%-0.1%, wherein the mass ratio of nano zinc oxide to nano titanium dioxide is 1:1-5.

[0024] In one embodiment of the present invention, the steps of preparing the coated tea leaves include:

[0025] Put the new tea into the nanomaterial coating solution for coating treatment. The soaking time is 30 to 60 minutes at a temperature of 25°C-30°C. During the coating process, a magnetic stirrer is used to continuously stir the solution at a speed of 200rpm.

[0026] It should be noted that the tea leaves are soaked in the coating solution and stirred by a magnetic stirrer to ensure that the nanoparticles are evenly distributed and fully coat the surface of the tea leaves, thereby increasing the activity of the tea leaves surface.

[0027] In one embodiment of the present invention, the step of preparing the irradiated tea leaves comprises: irradiating the coated tea leaves with light, using a light source with a wavelength of 200 to 500 nm, for a irradiation time of 1 to 5 minutes.

[0028] It should be noted that 1) Photocatalysis and antibacterial effects: Nano zinc oxide and nano titanium dioxide produce reactive oxygen species under ultraviolet light. These reactive oxygen species (ROS) can effectively promote the conversion of polyphenolic compounds in tea leaves, including gallic acid, and increase their content. At the same time, nanoparticles have antibacterial properties, which can inhibit the growth of adverse microorganisms and protect the effective ingredients in tea leaves. 2) Destructive effect: Nanoparticles and photocatalysis can destroy the cell walls of tea leaves, promote the release and conversion of internal components, especially the conversion of polyphenolic substances, including gallic acid.

[0029] In one embodiment of the present invention, the preparation step of the aged tea leaves includes: placing the tea leaves after light exposure in a closed humid and hot environment, the relative humidity of the humid and hot environment is 85%-95% RH, the temperature is 40°C~50°C, and the humid and hot treatment time is 12~24 hours.

[0030] It should be noted that the wet heat treatment provides an environment of high humidity and moderate temperature, which accelerates the reaction in the tea leaves and accelerates the aging process of the tea leaves. High humidity promotes the water activity in the tea leaves and increases the reaction rate; moderate temperature ensures the stability and efficiency of the reaction. This environment can further promote the reaction after photocatalytic treatment and significantly improve the conversion process of polyphenols in tea leaves.

[0031] In one embodiment of the present invention, the preparation method comprises the following steps:

[0032] dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution;

[0033] Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves;

[0034] Exposing the coated tea leaves to light to obtain illuminated tea leaves;

[0035] Aging the tea leaves after being exposed to light to obtain aged tea leaves;

[0036] Take aged tea leaves, clean them, add water and boil them in batches, the first time adding water is 8 to 12 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; the second time adding water is 6 to 10 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; the third time adding water is 4 to 8 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; after boiling is completed, combine the filtrate, filter to obtain a clarified filtrate, cool it, add distiller's grains and gallnut powder to obtain a mixture, add water and the mixture, stir into a soft block, ferment, take out when it is covered with white hairs, dry and cut into blocks to obtain Baiyaojian.

[0037] It should be noted that the fermentation period of Baiyaojian is generally marked by the appearance of "white hair" on the surface. This process usually takes seven days, and the "white hair" is the mycelium of microorganisms that has been fully fermented and grown vigorously.

[0038] In one embodiment of the present invention, the preparation method comprises the following steps:

[0039] dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution;

[0040] Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves;

[0041] Exposing the coated tea leaves to light to obtain illuminated tea leaves;

[0042] Aging the tea leaves after being exposed to light to obtain aged tea leaves;

[0043] Prepare the raw materials according to the ratio of 100:25:6.2 of gallnut: distiller's grains: aged tea leaves, clean and take the aged tea leaves, add water and boil them in batches. After boiling, combine the filtrate, filter to obtain a clarified filtrate, cool, add distiller's grains and gallnut powder to obtain a mixture, add water and stir the mixture into soft blocks, place in a suitable container, and ferment in a closed manner at a fermentation temperature of 25℃-30℃, a humidity of 70%-80%RH, and a fermentation time of 1-7 days. When it is covered with white hairs, take it out, dry it and cut it into blocks to obtain Baiyaojian.

[0044] It should be noted that 1) Gallic acid is rich in polyphenols, such as tannic acid and gallic acid, which have antioxidant and antibacterial effects. During the fermentation process, the components react to increase the conversion of polyphenols, thereby increasing the production of gallic acid. 2) Wine lees are rich in yeast and other beneficial microorganisms. These microorganisms produce a variety of enzymes during the fermentation process, which can catalyze the conversion of components in tea and gallic acid, and further promote the production of polyphenols. At the same time, the metabolites of microorganisms can also enhance the flavor and efficacy of tea. 3) In order to ensure the safety and medicinal purity of the raw materials, the raw materials need to be purified.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] (1) Different from the prior art, the present application uses aged tea leaves as one of the raw materials. The aged tea leaves do not use naturally fermented aged tea leaves. The method of the present application is used to age the tea leaves, which is convenient to select materials, has a wide range of materials, shortens the aging time, and thus shortens the overall process. Specifically, the present application first coats the tea leaves with a nanomaterial coating solution, then irradiates and ages them to obtain aged tea leaves. The nanomaterials are nano zinc oxide and / or nano titanium dioxide, and the coating material is chitosan solution. 1) Chitosan forms a thin film after dissolution, which can effectively coat the nanoparticles and improve the stability and dispersibility of the nanoparticles. This film not only protects the nanoparticles, but also prevents the agglomeration of the nanoparticles, ensuring that the nanoparticles can be evenly distributed on the surface of the tea leaves when coating new tea. 2) Nanoparticles, chitosan solution, and light can work synergistically: On the one hand, nano zinc oxide and nano titanium dioxide produce active oxygen species under ultraviolet light irradiation, and these active oxygen species can effectively promote the conversion of polyphenol compounds in tea leaves, including gallic acid substances, and increase their content. At the same time, nanoparticles have antibacterial properties, which can inhibit the growth of adverse microorganisms and protect the effective ingredients in tea. On the other hand, nanoparticles and photocatalysis can destroy cell structures such as tea cell walls, promote the release and transformation of internal components, especially the transformation of polyphenols, which include gallic acid. At the same time, chitosan solution can homogenize the components in the solution so that the tea leaves are evenly exposed to light. 3) During aging, wet heat treatment provides an environment of high humidity and moderate temperature, which accelerates the reaction in the tea leaves and accelerates the aging process of the tea leaves. High humidity promotes the activity of water in the tea leaves and increases the reaction rate; moderate temperature ensures the stability and efficiency of the reaction. This environment can further promote the reaction after photocatalytic treatment and significantly improve the transformation process of polyphenols in tea leaves. 4) When the aged tea leaves and gallnuts are fermented with lees, the aged tea leaves are more active and respond faster during fermentation. They can quickly "mobilize" gallnuts and lees, provide a better fermentation environment, shorten the dormancy time of microorganisms in the lees, and accelerate the transformation rate of the effective ingredients in gallnuts.

[0047] (2) By setting the examples and comparative examples, the present application found that: 1) The fermentation time of Baiyaojian can be shortened by aging the tea leaves first and then mixing them with gallnut and distiller's grains for fermentation. 2) The gallic acid content of Baiyaojian can be increased by aging the tea leaves first and then mixing them with gallnut and distiller's grains for fermentation. 3) When nano zinc oxide and nano titanium dioxide are used together, a synergistic effect can be produced, which can shorten the fermentation time of Baiyaojian. 4) When nano zinc oxide and nano titanium dioxide are used together, a synergistic effect can be produced, which can increase the gallic acid content of Baiyaojian. 5) The synergistic effect of nano zinc oxide and nano titanium dioxide may be because: the heterojunction structure of nano zinc oxide and nano titanium dioxide can effectively separate photogenerated electrons and holes, reduce their recombination, extend the life of carriers, and improve the photocatalytic efficiency. Nano zinc oxide and nano titanium dioxide can produce more active oxygen species and enhance the antibacterial effect. The reaction sites on the surface of tea leaves are increased, the surface activity is improved, the conversion of effective ingredients on the surface of tea leaves is further promoted, the aging process is accelerated, and the content of effective ingredients is increased. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] Unless otherwise specified, the equipment and materials used in the examples can be easily obtained from commercial companies.

[0050] Wine lees and Chinese gall powder do not affect the final effect or are not key influencing factors, and conventional commercial products are used.

[0051] In order to better reflect the aging and fermentation effects, the new tea / tea leaves in Examples 1 to 3 are green tea, which is not fermented.

[0052] Chitosan, Sigma-Aldrich, C3646;

[0053] Acetic acid, Sigma-Aldrich, A6283;

[0054] Nano zinc oxide, Hangzhou Hengna New Materials Co., Ltd., HN-JH05;

[0055] Nano titanium dioxide, Hangzhou Hengna New Materials Co., Ltd., CAS#:13463-67-7.

[0056] In addition, "cleaning" and "cutting into pieces after drying to obtain Baiyao Decoction" do not affect the final effect or are not key influencing factors, and are well-known means, so they are not elaborated.

[0057] The term "tea leaves a few months after production" used below means the time from tea production to use. It can also be understood as tea leaves a few months after production, where several months means storage time. For example, new tea leaves three months after production means new tea leaves that have been stored for three months after production.

[0058] Example 1

[0059] The preparation method of Wumen's special Baiyaojian is as follows:

[0060] 1. Preparation of Chitosan Solution

[0061] 10 g of chitosan was dissolved in 1 liter of 2% acetic acid solution, and stirred with a magnetic stirrer for 30 minutes until the chitosan was completely dissolved to obtain a chitosan solution.

[0062] 2. Nanomaterial coating treatment

[0063] 0.5 g of nano zinc oxide and 0.5 g of nano titanium dioxide were put into 1 liter of chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency was set to 20 kHz and the dispersion time was 30 minutes to obtain a nano material coating solution.

[0064] 3. Put 100g of new tea produced 3 months after production into 1 liter of nanomaterial coating solution for 45 minutes, and control the temperature at 28°C. During the coating process, use a magnetic stirrer to continuously stir the solution at a speed of 200rpm. After coating, take out the tea leaves and place them on a drying rack to dry to obtain coated tea leaves.

[0065] 4. Expose the coated tea leaves to light using a light source with an ultraviolet wavelength of 365 nm and controlling the exposure time to 3 minutes to obtain illuminated tea leaves.

[0066] 5. Place the tea leaves after light exposure in a wet heat test chamber, and control the relative humidity of the wet heat environment at 95% RH and the temperature at 45° C. The wet heat treatment time is 18 hours to obtain aged tea leaves.

[0067] 6. Prepare the raw materials according to the ratio of 100:25:6.2 of gallnut: distiller's grains: aged tea leaves, and purify the raw materials at the same time.

[0068] Take 6.2g of aged tea leaves, add water and boil them in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the boiling is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into a soft block, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0069] Example 2

[0070] The preparation method of Wumen Special Baiyao Decoction is different from that of Example 1. In this example, in step 2, 2. Nanomaterial coating treatment

[0071] 1 g of nano zinc oxide was placed in 1 liter of chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency was set to 20 kHz and the dispersion time was 30 minutes to obtain a nano material coating solution.

[0072] The rest is the same as Example 1.

[0073] Example 3

[0074] The preparation method of Wumen Special Baiyao Decoction is different from that of Example 1. In this example, in step 2, 2. Nanomaterial coating treatment

[0075] 1 g of nano-titanium dioxide was placed in 1 liter of chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency was set to 20 kHz and the dispersion time was 30 minutes to obtain a nano-material coating solution.

[0076] The rest is the same as Example 1.

[0077] Comparative Example 1

[0078] The preparation method of Baiyaojian is as follows:

[0079] The raw materials are prepared in the ratio of 100:25:6.2 of gallnut: distiller's grains: tea leaves, and the raw materials are purified at the same time. The tea leaves are new tea 3 months after production, and the new tea is green tea.

[0080] Take 6.2g tea leaves, add water and boil in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the decoction is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0081] Comparative Example 2

[0082] The preparation method of Baiyaojian is as follows:

[0083] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0084] The tea leaves are aged 15 months after production. Aged tea is naturally aged green tea.

[0085] Take 6.2g tea leaves, add water and boil in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the decoction is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0086] Comparative Example 3

[0087] The preparation method of Baiyaojian is as follows:

[0088] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0089] The tea leaves are aged 27 months after production. Aged tea is naturally aged green tea.

[0090] Take 6.2g tea leaves, add water and boil in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the decoction is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0091] Comparative Example 4

[0092] The preparation method of Baiyaojian is as follows:

[0093] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0094] The tea leaves are aged 39 months after production. Aged tea is naturally aged green tea.

[0095] Take 6.2g tea leaves, add water and boil in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the decoction is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0096] Comparative Example 5

[0097] The preparation method of Baiyaojian is as follows:

[0098] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0099] The tea leaves are aged 51 months after production. Aged tea is naturally aged green tea.

[0100] Take 6.2g tea leaves, add water and boil for 30 minutes in batches, add 62ml distilled water for the first time, boil for 30 minutes; add 49ml distilled water for the second time, boil for 20 minutes. After boiling, filter, combine the filtrate, obtain a clarified filtrate, cool, add 25g lees and 100g gallnut powder, obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place in a suitable container, ferment in a closed manner, control the fermentation temperature between 28℃, control the humidity at 80%RH, ferment, and when "covered with white hair", record the fermentation time, take out, dry and cut into blocks, and obtain Baiyaojian.

[0101] Comparative Example 6

[0102] The preparation method of Baiyaojian is as follows:

[0103] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0104] The tea leaves are new tea 3 months after production. New tea is black tea.

[0105] Take 6.2g tea leaves, add water and boil for 30 minutes in batches, add 62ml distilled water for the first time, boil for 30 minutes; add 49ml distilled water for the second time, boil for 20 minutes. After boiling, filter, combine the filtrate, obtain a clarified filtrate, cool, add 25g lees and 100g gallnut powder, obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place in a suitable container, ferment in a closed manner, control the fermentation temperature between 28℃, control the humidity at 80%RH, ferment, and when "covered with white hair", record the fermentation time, take out, dry and cut into blocks, and obtain Baiyaojian.

[0106] Comparative Example 7

[0107] The preparation method of Baiyaojian is as follows:

[0108] Prepare the raw materials according to the ratio of gallnut: distiller's grains: tea leaves of 100:25:6.2, and purify the raw materials at the same time.

[0109] The tea leaves are aged 51 months after production. Aged tea is black tea.

[0110] Take 6.2g tea leaves, add water and boil for 30 minutes in batches, add 62ml distilled water for the first time, boil for 30 minutes; add 49ml distilled water for the second time, boil for 20 minutes. After boiling, filter, combine the filtrate, obtain a clarified filtrate, cool, add 25g lees and 100g gallnut powder, obtain a mixture, add appropriate amount of water and stir the mixture into soft blocks, place in a suitable container, ferment in a closed manner, control the fermentation temperature between 28℃, control the humidity at 80%RH, ferment, and when "covered with white hair", record the fermentation time, take out, dry and cut into blocks, and obtain Baiyaojian.

[0111] Comparative Example 8

[0112] The preparation method of Baiyaojian is as follows:

[0113] 1. Nanomaterial coating treatment

[0114] 0.5 g of nano zinc oxide and 0.5 g of nano titanium dioxide were put into 1 liter of distilled water and dispersed using an ultrasonic disperser. The ultrasonic frequency was set to 20 kHz and the dispersion time was 30 minutes to obtain a nano material coating solution.

[0115] 3. Put 100g of new tea produced 3 months after production into 1 liter of nanomaterial coating solution, soak for 45 minutes, and control the temperature at 28°C. During the coating process, use a magnetic stirrer to continuously stir the solution at a speed of 200rpm. After coating, take out the tea leaves and place them on a drying rack to dry to obtain coated tea leaves. Among them, the new tea leaves are green tea.

[0116] 4. Expose the coated tea leaves to light using a light source with an ultraviolet wavelength of 365 nm and controlling the exposure time to 3 minutes to obtain illuminated tea leaves.

[0117] 5. Place the tea leaves after light exposure in a wet heat test chamber, and control the relative humidity of the wet heat environment at 95% RH and the temperature at 45° C. The wet heat treatment time is 18 hours to obtain aged tea leaves.

[0118] 6. Prepare the raw materials according to the ratio of 100:25:6.2 of gallnut: distiller's grains: aged tea leaves, and purify the raw materials at the same time.

[0119] Take 6.2g of aged tea leaves, add water and boil them in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the boiling is completed, filter, combine the filtrate, obtain a clarified filtrate, cool it, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add appropriate amount of water and stir the mixture into a soft block, place it in a suitable container, and ferment it in a closed manner. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment, when it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian.

[0120] Comparative Example 9

[0121] The preparation method of Baiyaojian is as follows:

[0122] 1. 100g of new tea produced 3 months after production is irradiated with light, using a light source with an ultraviolet wavelength of 365nm, and the irradiation time is controlled to be 3 minutes to obtain tea leaves after irradiation. Among them, the tea leaves of the new tea are green tea.

[0123] 2. Place the tea leaves after light exposure in a wet heat test chamber, and control the relative humidity of the wet heat environment at 95% RH and the temperature at 45° C. The wet heat treatment time is 18 hours to obtain aged tea leaves.

[0124] 3. Prepare the raw materials according to the ratio of 100:25:6.2 of gallnut: distiller's grains: aged tea leaves, and purify the raw materials at the same time.

[0125] Take 6.2g of aged tea leaves, add water and boil in batches. For the first time, add 62ml of distilled water and boil for 30 minutes; for the second time, add 49ml of distilled water and boil for 20 minutes; for the third time, add 37ml of distilled water and boil for 15 minutes. After the decoction is completed, filter, combine the filtrate, obtain a clarified filtrate, cool, add 25g of wine lees and 100g of gallnut powder to obtain a mixture, add an appropriate amount of water and stir the mixture into a soft block, place it in a suitable container, and ferment it in a closed container. The fermentation temperature is controlled between 28℃ and the humidity is controlled at 80%RH. Ferment until it is "covered with white hair", record the fermentation time, take it out, dry it and cut it into pieces to obtain Baiyaojian. Samples of Examples 1 to 3 and Comparative Examples 1 to 9 were taken and tested:

[0126] Gallic acid content: Referring to the content determination method in the "Jiangsu Province Traditional Chinese Medicine Decoction Processing Specifications (2020 Edition)", the gallic acid content is required to be no less than 40%.

[0127] Fermentation time: When the dough is covered with white hair, record the fermentation time.

[0128] The results are shown in Table 1.

[0129] Table 1

[0130]

[0131]

[0132] Comment: Referring to Table 1, Examples 1 to 3 of the present application successfully prepared the Wumen Specialty Baiyao Decoction.

[0133] By comparing Examples 1 to 3, it is found that when nano zinc oxide and nano titanium dioxide are added simultaneously, the fermentation time can be further shortened and the gallic acid content can be increased.

[0134] Comparing Comparative Examples 1 to 7, it was found that 1) aging the tea leaves first and then mixing them with gallnut and distiller's grains for fermentation can shorten the fermentation time of Baiyaojian and increase the gallic acid content of Baiyaojian. 2) If unfermented green tea is selected, within a specific aging time, the longer the aging time, the shorter the fermentation time of Baiyaojian and the higher the gallic acid content. 3) If fermented tea leaves, such as black tea, are selected, the fermentation time of Baiyaojian can be shortened and the gallic acid content can be increased.

[0135] By comparing Examples 1 to 3 with Comparative Examples 1 to 7, it was found that 1) compared with naturally aged old tea, tea aged by the aging method of the present application can not only shorten the aging time of tea, but also shorten the fermentation time of Baiyaojian and increase the gallic acid content of Baiyaojian; 2) even if the black tea is 51 months after production and is a fermented tea, its performance (fermentation time and gallic acid content) when used in the preparation of Baiyaojian is not as good as that of the present application.

[0136] Comparing Examples 1 to 3 with Comparative Example 8, it was found that when nanoparticles were dispersed in conventional coating materials, they were not as good as chitosan solution, and chitosan solution could cooperate with other components to improve the test effect. Comparing Examples 1 to 3 with Comparative Example 9, it was found that direct illumination without treating new tea could shorten the fermentation time of Baiyaojian and increase the gallic acid content of Baiyaojian, but it was far inferior to the test effect of Examples 1 to 3. It further proves that the nanoparticles, chitosan solution and illumination in this application can synergistically improve the test effect.

[0137] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. The preparation method of Wumen Special Baiyao Decoction is characterized by: The steps include: dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution; Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves; Exposing the coated tea leaves to light to obtain illuminated tea leaves; Aging the tea leaves after being exposed to light to obtain aged tea leaves; Take aged tea leaves, add water to them in batches and boil them. After boiling, combine the filtrates, filter them to obtain clarified filtrates, cool them, add distiller's grains and gallnut powder to obtain a mixture, then add water to the mixture and stir it into soft blocks. Ferment the mixture until it is covered with white hairs, take it out, dry it and cut it into blocks to obtain Baiyaojian.

2. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The nanoparticles include at least one of nano zinc oxide and nano titanium dioxide.

3. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The coating material includes chitosan solution.

4. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The preparation steps of the nano material coating solution include: Dissolve chitosan in acetic acid solution with a concentration of 2% to 5%, and stir until completely dissolved to obtain a chitosan solution with a concentration of 2% to 5%; The nanoparticles are placed in a chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency is set to 10 to 30 kHz and the dispersion time is 10 to 50 minutes to obtain a nanomaterial coating solution with a concentration of 0.01% to 1%.

5. The method for preparing Wumen Special Baiyao Decoction according to claim 2, characterized in that: The preparation steps of the nano material coating solution include: Dissolve chitosan in acetic acid solution with a concentration of 2% to 5%, and stir until completely dissolved to obtain a chitosan solution with a concentration of 2% to 5%; Nano zinc oxide and nano titanium dioxide are placed in a chitosan solution and dispersed using an ultrasonic disperser. The ultrasonic frequency is set to 10-30 kHz and the dispersion time is 10-50 minutes to obtain a nano material coating solution with a concentration of 0.01%-1%, wherein the mass ratio of nano zinc oxide to nano titanium dioxide is 1:1-5.

6. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The steps of preparing the coated tea leaves include: Put the new tea into the nanomaterial coating solution for coating treatment. The soaking time is 30 to 60 minutes at a temperature of 25°C to 30°C. During the coating process, a magnetic stirrer is used to continuously stir the solution at a speed of 200rpm.

7. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The steps of preparing the tea leaves after illumination include: The coated tea leaves are irradiated with light using a light source with a wavelength of 200 to 500 nm for 1 to 5 minutes.

8. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The preparation step of the aged tea leaves comprises: placing the tea leaves after light exposure in a closed hot and humid environment, wherein the relative humidity of the hot and humid environment is 85% to 95% RH, the temperature is 40° C. to 50° C., and the hot and humid treatment time is 12 to 24 hours.

9. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The steps include: dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution; Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves; Exposing the coated tea leaves to light to obtain illuminated tea leaves; Aging the tea leaves after being exposed to light to obtain aged tea leaves; Take aged tea leaves, add water and boil them in batches, the first time adding water is 8 to 12 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; the second time adding water is 6 to 10 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; the third time adding water is 4 to 8 times the weight of the tea leaves, and boiling is for 10 to 50 minutes; after boiling is completed, combine the filtrates, filter to obtain clarified filtrates, cool, add distiller's grains and gallnut powder to obtain a mixture, add water and the mixture, stir into soft blocks, ferment, take out when covered with white hairs, dry and cut into blocks to obtain Baiyaojian.

10. The method for preparing Wumen Special Baiyao Decoction according to claim 1, characterized in that: The steps include: dispersing the nanoparticles in the coating material to obtain a nanomaterial coating solution; Putting new tea into the nanomaterial coating solution, fully soaking it, and drying it after coating to obtain coated tea leaves; Exposing the coated tea leaves to light to obtain illuminated tea leaves; Aging the tea leaves after being exposed to light to obtain aged tea leaves; Prepare the raw materials according to the ratio of 100:25:6.2 of gallnut: distiller's grains: aged tea leaves, clean and take the aged tea leaves, add water and boil them in batches. After boiling, combine the filtrate, filter to obtain a clarified filtrate, cool, add distiller's grains and gallnut powder to obtain a mixture, add water and stir the mixture into soft blocks, place in a suitable container, and ferment in a closed manner at a fermentation temperature of 25℃~30℃, a relative humidity of 70%~80%RH, and a fermentation time of 1~7 days. When it is covered with white hairs, take it out, dry and cut into blocks to obtain Baiyaojian.

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